Wie süß ispt der den bitte
July 31, 2025 at 5:34 AM
Wie süß ispt der den bitte
The Uracil-DNA Glycosylase2 UNG2 is degraded via a novel N-degron | CTLH-MKLN1-FAM72A E3 ligase recognizes the N-terminally acetylated UNG2 | @ispt-proteinterm.bsky.social
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
September 22, 2026 at 11:41 AM
The Uracil-DNA Glycosylase2 UNG2 is degraded via a novel N-degron | CTLH-MKLN1-FAM72A E3 ligase recognizes the N-terminally acetylated UNG2 | @ispt-proteinterm.bsky.social
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
A whole universe of protein N-terminal modifications and their biological impact! Thanks to all co-authors! @cp-trendsbiochem.bsky.social @ispt-proteinterm.bsky.social #oxidation #acetylation #methylation #myristoylation #arginylation #ubiquitylation www.sciencedirect.com/science/arti...
January 20, 2025 at 2:03 PM
A whole universe of protein N-terminal modifications and their biological impact! Thanks to all co-authors! @cp-trendsbiochem.bsky.social @ispt-proteinterm.bsky.social #oxidation #acetylation #methylation #myristoylation #arginylation #ubiquitylation www.sciencedirect.com/science/arti...
Protein N-terminal modifications - Feature Review and cover in the recent issue of @cp-trendsbiochem.bsky.social Thanks to all authors, Editor @sanniejculbertson.bsky.social and reviewers for great work and support! @ispt-proteinterm.bsky.social www.cell.com/trends/bioch...
April 3, 2025 at 2:54 PM
Protein N-terminal modifications - Feature Review and cover in the recent issue of @cp-trendsbiochem.bsky.social Thanks to all authors, Editor @sanniejculbertson.bsky.social and reviewers for great work and support! @ispt-proteinterm.bsky.social www.cell.com/trends/bioch...
Structural and mechanistic insights into the UBR4-KCMF1-Calmodulin complex @ispt-proteinterm.bsky.social academic.oup.com/proteincell/...
Structural and mechanistic insights into the UBR4-KCMF1-Calmodulin complex
Dear Editor,
academic.oup.com
June 23, 2026 at 9:20 AM
Structural and mechanistic insights into the UBR4-KCMF1-Calmodulin complex @ispt-proteinterm.bsky.social academic.oup.com/proteincell/...
DEGRONOPEDIA - a web server for identifying and predicting protein degradation signals across eukaryotic proteomes. Detailed guide by Natalia Szulc @lumirare.com and Wojciech Pokrzywa @wpokrzywa.bsky.social in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
July 9, 2025 at 8:32 AM
DEGRONOPEDIA - a web server for identifying and predicting protein degradation signals across eukaryotic proteomes. Detailed guide by Natalia Szulc @lumirare.com and Wojciech Pokrzywa @wpokrzywa.bsky.social in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
On the degradation of damaged cellular proteins via C-terminal amidation. By Alfred Freeberg & @micharapelab.bsky.social. @ispt-proteinterm.bsky.social www.nature.com/articles/d41...
Signs of damage that drive protein degradation
How the stress-response machinery eliminates damaged proteins.
www.nature.com
January 29, 2025 at 5:34 PM
On the degradation of damaged cellular proteins via C-terminal amidation. By Alfred Freeberg & @micharapelab.bsky.social. @ispt-proteinterm.bsky.social www.nature.com/articles/d41...
Actin N-terminal maturation can be blocked by a butynamide stereoprobe which acts as an ACTMAP inhibitor. Preprint by Xiong et al. Benjamin Cravatt and Bruno Melillo. @ispt-proteinterm.bsky.social
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
February 23, 2026 at 8:32 AM
Actin N-terminal maturation can be blocked by a butynamide stereoprobe which acts as an ACTMAP inhibitor. Preprint by Xiong et al. Benjamin Cravatt and Bruno Melillo. @ispt-proteinterm.bsky.social
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
N-terminal cysteine dependent proteolysis of RGS4/5 is not sensitive to physiological oxidative stress | Preprint by Ya-Min Tian, Haeun Kim, Peter Ratcliffe & Thomas Keeley @ox.ac.uk @ispt-proteinterm.bsky.social #N-degron #cysteine www.biorxiv.org/content/10.6...
www.biorxiv.org
April 22, 2026 at 5:13 PM
N-terminal cysteine dependent proteolysis of RGS4/5 is not sensitive to physiological oxidative stress | Preprint by Ya-Min Tian, Haeun Kim, Peter Ratcliffe & Thomas Keeley @ox.ac.uk @ispt-proteinterm.bsky.social #N-degron #cysteine www.biorxiv.org/content/10.6...
Want to assess N-terminal acetyltransferases without radioactive labelling or mass spec? Look here! @ispt-proteinterm.bsky.social @cellsignal.com
October 27, 2025 at 8:25 PM
Want to assess N-terminal acetyltransferases without radioactive labelling or mass spec? Look here! @ispt-proteinterm.bsky.social @cellsignal.com
Urgent pilot report at 1800Z: ISP UUA /OV ISPT/TM 1800/FL014/TP A320/RM +10 KTS AND LIGHT TURB RWY 06 FINAL
September 25, 2026 at 6:02 PM
Urgent pilot report at 1800Z: ISP UUA /OV ISPT/TM 1800/FL014/TP A320/RM +10 KTS AND LIGHT TURB RWY 06 FINAL
How to define degrons and E3 ligase specificities by peptide pulldowns | Methods in Enzymology | Kwon and Ji labs | #KCMF1 #Cysteine #Arg/N-degron @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
August 7, 2025 at 7:11 AM
How to define degrons and E3 ligase specificities by peptide pulldowns | Methods in Enzymology | Kwon and Ji labs | #KCMF1 #Cysteine #Arg/N-degron @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
Coenzyme A biosynthesis is regulated by two distinct Ubiquitin E3 ligase complexes | UBR4-KCMF1 and MKLN1-CTLH independently converge on a common N-terminal MK Arg/N-degron to promote PANK degradation and restrict CoA biosynthesis | @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.6...
Parallel Arg/N-degron recognition systems regulate coenzyme A biosynthesis
Coenzyme A (CoA) is an essential metabolic cofactor whose biosynthesis is controlled by pantothenate kinases (PANKs), the rate-limiting enzymes in CoA synthesis. Although CoA production is extensively...
www.biorxiv.org
September 21, 2026 at 7:44 AM
Coenzyme A biosynthesis is regulated by two distinct Ubiquitin E3 ligase complexes | UBR4-KCMF1 and MKLN1-CTLH independently converge on a common N-terminal MK Arg/N-degron to promote PANK degradation and restrict CoA biosynthesis | @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.6...
How to detect protein N-terminal arginylation - a key step of protein degradation via the Arg/N-degron pathway. By Jasper Eising and @ndissmeyer.bsky.social in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
July 7, 2025 at 6:50 PM
How to detect protein N-terminal arginylation - a key step of protein degradation via the Arg/N-degron pathway. By Jasper Eising and @ndissmeyer.bsky.social in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
A majority of eukaryotic proteins are N-terminally acetylated. Here is an updated review of the responsible enzymes and the impact of this modification for proteins, cells and organisms @naturecomms.bsky.social @ispt-proteinterm.bsky.social #acetylation #N-termini #N-degron rdcu.be/d6mbd
January 15, 2025 at 4:33 PM
A majority of eukaryotic proteins are N-terminally acetylated. Here is an updated review of the responsible enzymes and the impact of this modification for proteins, cells and organisms @naturecomms.bsky.social @ispt-proteinterm.bsky.social #acetylation #N-termini #N-degron rdcu.be/d6mbd
Tideglusib as a First-in-Class Chemical Probe for the major N-terminal acetyltransferase NatA | By Rong Huang's lab and collaborators @ispt-proteinterm.bsky.social pubs.acs.org/acbcct/artic...
September 13, 2026 at 6:46 AM
Tideglusib as a First-in-Class Chemical Probe for the major N-terminal acetyltransferase NatA | By Rong Huang's lab and collaborators @ispt-proteinterm.bsky.social pubs.acs.org/acbcct/artic...
How to use bacteria for arginylation assessment. Co-expression of ATE1 and substrate outlined by Yi Zhang and colleagues in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
July 8, 2025 at 7:18 AM
How to use bacteria for arginylation assessment. Co-expression of ATE1 and substrate outlined by Yi Zhang and colleagues in Methods in Enzymology. @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
Developing leaves form a spatiotemporal oxygen gradient that is sensed through the oxygen sensing machinery and directs morphogenesis | New preprint from @daanweits.bsky.social and @syno2xis.bsky.social labs #N-degron #Cysteine #hypoxia @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...
September 23, 2025 at 7:38 AM
Developing leaves form a spatiotemporal oxygen gradient that is sensed through the oxygen sensing machinery and directs morphogenesis | New preprint from @daanweits.bsky.social and @syno2xis.bsky.social labs #N-degron #Cysteine #hypoxia @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.1...
Assessing protein N-terminal myristoylation. Detailed protocols by Ed Tate's group in Methods in Enzymology. #lipidation @ispt-proteinterm.bsky.social @imperialcollegeldn.bsky.social www.sciencedirect.com/science/arti...
August 6, 2025 at 7:53 AM
Assessing protein N-terminal myristoylation. Detailed protocols by Ed Tate's group in Methods in Enzymology. #lipidation @ispt-proteinterm.bsky.social @imperialcollegeldn.bsky.social www.sciencedirect.com/science/arti...
Give it to me straight, Footy Prime!
We Do!
Let the Unpacking Begin+Rd. of 32 Beckons!
#CANMNT #CanadaSoccer #LACity
#FIFAWorldCup
GET IN!
Youtu.be/ISpt-cKuIqk💻
Full Paudio:
podcasts.apple.com/ca/podcast/foo…���
We Do!
Let the Unpacking Begin+Rd. of 32 Beckons!
#CANMNT #CanadaSoccer #LACity
#FIFAWorldCup
GET IN!
Youtu.be/ISpt-cKuIqk💻
Full Paudio:
podcasts.apple.com/ca/podcast/foo…���
https://tu.be/ISpt-cKuIqk💻
June 25, 2026 at 3:41 PM
Give it to me straight, Footy Prime!
We Do!
Let the Unpacking Begin+Rd. of 32 Beckons!
#CANMNT #CanadaSoccer #LACity
#FIFAWorldCup
GET IN!
Youtu.be/ISpt-cKuIqk💻
Full Paudio:
podcasts.apple.com/ca/podcast/foo…���
We Do!
Let the Unpacking Begin+Rd. of 32 Beckons!
#CANMNT #CanadaSoccer #LACity
#FIFAWorldCup
GET IN!
Youtu.be/ISpt-cKuIqk💻
Full Paudio:
podcasts.apple.com/ca/podcast/foo…���
Global profiling of N-terminal cysteine–dependent degradation mechanisms | PNAS | @ispt-proteinterm.bsky.social www.pnas.org/doi/10.1073/...
Global profiling of N-terminal cysteine–dependent degradation mechanisms | PNAS
Hypoxia, a condition characterized by insufficient oxygen supply, challenges cellular
homeostasis and energy production, triggering adaptive respon...
www.pnas.org
August 18, 2025 at 1:09 PM
Global profiling of N-terminal cysteine–dependent degradation mechanisms | PNAS | @ispt-proteinterm.bsky.social www.pnas.org/doi/10.1073/...
Naturally: the developer is going to planting some saplings and claiming that 'offsets' the impacts; also they claim to be "carbon neutral"
www.abc.net.au/news/2025-08...
www.abc.net.au/news/2025-08...
September 3, 2025 at 10:39 AM
Naturally: the developer is going to planting some saplings and claiming that 'offsets' the impacts; also they claim to be "carbon neutral"
www.abc.net.au/news/2025-08...
www.abc.net.au/news/2025-08...
Mechanism for voltage-gated channel inactivation uncovered | N-terminal acetylation of the intracellular domain of the Shaker potassium channel is key for plugging the pore | By Kenton Swartz lab in @nature.com @ispt-proteinterm.bsky.social www.nature.com/articles/s41...
August 7, 2025 at 8:11 AM
Mechanism for voltage-gated channel inactivation uncovered | N-terminal acetylation of the intracellular domain of the Shaker potassium channel is key for plugging the pore | By Kenton Swartz lab in @nature.com @ispt-proteinterm.bsky.social www.nature.com/articles/s41...
Expression and purification of ribosome associated factors: Methionine aminopeptidases and N-terminal acetyltransferases | Methods in Enzymology | Marius Klein and Irmgard Sinning @uniheidelberg.bsky.social @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
July 29, 2025 at 7:46 AM
Expression and purification of ribosome associated factors: Methionine aminopeptidases and N-terminal acetyltransferases | Methods in Enzymology | Marius Klein and Irmgard Sinning @uniheidelberg.bsky.social @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
How to express, purify and assess the activity of the ATE1 arginyltransferase | Methods in Enzymology | Yi Zhang lab @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...
July 29, 2025 at 7:38 AM
How to express, purify and assess the activity of the ATE1 arginyltransferase | Methods in Enzymology | Yi Zhang lab @ispt-proteinterm.bsky.social www.sciencedirect.com/science/arti...