Antibiotics can be administered via multiple routes in the clinic and in experiments. Yet, whether the administration route affects treatment outcomes is unclear. To test this, we treated mice reared in a specific pathogen-free (SPF) barrier facility with four commonly used antibiotics (that is, vancomycin, ampicillin, metronidazole and neomycin), either separately or combined (a four-antibiotic mix). Antibiotics were administered orally via gavage or systemically via intraperitoneal (i.p.) or intravenous (i.v.) injection. Mimicking real-world conditions, mice were treated twice a day for 3.5 days (to reflect real-world observations and the shift towards shorter regimen periods22,23,24,25,26), after which stool and colonic tissue samples were collected (Fig. 1a). Faecal samples were also collected before treatment, to establish a baseline. We first analysed the bacterial composition of faecal samples using 16S ribosomal RNA (rRNA) amplicon sequencing. We calculated the effective number of genera (using the Shannon diversity index27) and performed a pairwise community-level comparison of the effect of the antibiotic treatment using Adonis2 (ref. 28) (Fig. 1b,c). We detail our observations on the effects of ampicillin, metronidazole, neomycin and a mix of these three antibiotics on microbial composition in Extended Data Fig. 1. Herein, we focus on the results relating to vancomycin. Contrary to...

Abstract. Hypothyroidism decreases the basal metabolic rate. Using three models of peritonitis, intraperitoneal LPS plus Zymosan challenge, cecal slurry-in

Nelfbfl/fl mice61 were a gift from R. Li. AID-cre mice62 were provided by M. Muschen and were used as heterozygotes. Experimental mice were generated by intercrossing AID-cre and Nelfbfl/fl mice to generate Nelfbfl/fl;AID-cre and control Nelfbfl/+;AID-cre mice. Both male and female mice were used in experiments. Splenic GCB cells were sorted as the B220+PNAhiCD95hi population 7 days after intraperitoneal injection of sheep red blood cells followed by mutation sequencing analysis. Animal studies were performed without randomization, investigator blinding or previous analyses of sample size. All animal studies were conducted in accordance with national guidelines and were approved by the Yale Institutional Animal Care and Use Committee. Cell culture Ramos cells and cell lines derived from Ramos cells were grown in RPMI-1640 (Gibco, 11875) supplemented with 10% FBS (Gemini) and 0.5 mg ml–1 penicillin–streptomycin–glutamine (Gibco, 10378016) at 37 °C, 5% CO2. Ramos cells have been used extensively for the study of SHM factors and mechanisms60,63,64,65,66,67,68,69,70,71,72,73,74,75,76. 293T cells were grown at 37 °C, 5% CO2 in DMEM (Gibco, 10566) supplemented in the same manner as Ramos cells. CH12F3 cells were cultured in RPMI 1640 medium supplemented with 10% FBS (Gibco), 1× MEM non-essential amino acids, 10 mM HEPES, 2 mM Glutamax, 1...

To define the transcriptional and epigenetic landscape of the adult cardiac injury response to SDH inhibition, we used our established pharmacological model of malonate treatment after MI6. Adult male mice (8 weeks old) underwent sham or MI surgery followed by 14 daily intraperitoneal (i.p.) injections of saline or dimethyl malonate (Fig. 1a; treatment groups: saline sham, malonate sham, saline MI and malonate MI; four pooled hearts per group). We performed integrated snRNA-seq and snATAC-seq on these hearts and, following quality control, we jointly embedded both modalities to resolve cellular states across conditions (Fig. 1b,c and Extended Data Fig. 1a–d). Cell types were annotated using established markers in combination with differential gene and peak analysis, before subclustering major populations with >800 nuclei (CMs, cardiac fibroblasts (CFs), endothelial cells (ECs), mural cells and myeloid cells) to resolve cellular states17,18,19,20,21,22,23 (Fig. 1d, Extended Data Fig. 1e–g and Supplementary Table 1). Each major cell type showed treatment condition-predominant subclusters (>50% of population; Fig. 1e). Saline MI versus malonate MI differential expression and Gene Ontology (GO) revealed enrichment of profibrotic and extracellular matrix programs in saline MI populations, with the strongest gene expression and GO q values being in CFs, whereas malonate MI populations were...

Lantern Pharma (LTRN) received a USPTO Notice of Allowance for a U.S. patent on LP‑284 treatment methods for mantle cell lymphoma (MCL), double‑hit lymphoma (DHL), and other blood cancers, with expected protection into 2042 once issued.The allowed claims cover LP‑284 as a monotherapy and in combination with specified anti‑cancer agents, and include intravenous and intraperitoneal administration routes. This method‑of‑treatment patent will layer on top of existing LP‑284 composition‑of‑matter patents that protect the drug into 2039 across major markets including the United States, European Union, Japan, China, India, Mexico, Korea, and Australia. LP‑284 holds three FDA Orphan Drug Designations and is in an ongoing Phase 1a trial (NCT06132503) in relapsed or refractory B‑cell non‑Hodgkin's lymphomas and solid tumors, supported by preclinical potency data and an estimated $4 billion annual global blood cancer market opportunity.

HIPEC é a quimioterapia aquecida que combina cirurgia e tratamento intraperitoneal. Veja como salvou enfermeira com câncer avançado

Author summary Alveolar echinococcosis (AE) is a severe disease caused by the larval stage of the fox tapeworm Echinococcus multilocularis. The parasite forms tumor-like lesions in the liver and can s...

YouTube video by Mercy Medical Center - Baltimore

Echinococcosis is a zoonotic disease caused by cestodes of the genus Echinococcus. Alveolar echinococcosis (AE), caused by E. multilocularis, primarily affects the liver and shows infiltrative, tum...

The global aging trend highlights the critical challenge of sarcopenia, which severely compromises both quality of life and overall survival time. This study aimed to investigate the effects of Antarctic krill oil (KO) on accelerated aging-induced skeletal muscle atrophy and its underlying mechanisms. An accelerated aging mouse model was established via daily intraperitoneal injection of 400 mg/kg D-galactose (D-gal) for 12 consecutive weeks. Concurrently, mice received oral gavage of KO or fish oil (FO) at 400 mg/kg once daily. KO treatment mitigated D-gal-induced skeletal muscle atrophy and accelerated aging in mice, as evidenced by the attenuation of D-gal-induced declines in skeletal muscle mass, function, and muscle fiber cross-sectional area, as well as the downregulation of senescence markers. Notably, KO showed superior efficacy to FO. Moreover, KO treatment directly alleviated the D-gal-induced increase in senescence markers in C2C12 myotubes and mitigated the decrease in myotube diameter. Integrated transcriptomic and functional analyses identified AMP-activated protein kinase (AMPK), a master regulator of mitochondrial homeostasis, as a key target of KO in preventing accelerated aging-induced skeletal muscle atrophy. Molecular docking simulations further suggested plausible direct interactions between major KO components and AMPK subunits. Critically, genetic silencing of AMPK abolished the protective effects of KO...