Our openSPIM is officially up and running to image the latest catch of the day! Fueled by pymmcore-plus & pymmcore-gui! 💪⚡🔬 pymmcore-plus.github.io/pymmcore-plus/ github.com/pymmcore-plu...

@fedegasparoli.bsky.social is setting up our openSPIM in preparation for the light sheet lab next week. Pro tip: Stouts block light more effectively than lagers.

Endre Joachim Lerheim Mossige, et al.: From OpenSPIM to FlowSPIM: Enhancing the versatility of a light sheet microscope through an iterative design process https://arxiv.org/abs/2609.31115 https://arxiv.org/pdf/2609.31115 https://arxiv.org/html/2609.31115

A DIY high-resolution light-sheet microscope! Congrats to @john-haug.bsky.social, who simulated, designed, built, and validated the system!

Resolution: 235 nm (x), 233 nm (y), 350 nm (z). On par with LLSM, but easier to build. 🔥

www.biorxiv.org/content/10.1...
#LightSheet #Microscopy

@mpi-cbg.de in #Dresden has one of the most comprehensive line-ups of lightsheet microscopes in daily use. This will be enhanced by the few commercial systems that we are missing, plus the various open access set-ups such as #OpenSPIM and #Flamingo.

Average acquisition time of our #lightsheet microscopes: 18h/d over 8d. Data loss: zero #openspim #zeiss #EMBOplant live imaging course

[ATTENZIONE!]

The grand opening of "The United Forums of Bioimage Analysis"

https://forum.image.sc/

BoneJ
CellProfiler
Cytomine
Fiji
Icy
Ilastik
ImageJ
OpenSPIM
scikit-image
SLIM Curve
... but not limited to these tools.

For ANY questions! #NEUBIAS

openSPIM: 'IKEA/Lego style assembly in 19steps' @PavelTomancak #EuBIAS2013

Currently listening to the webinar. From Q&A: -30°C cooling, 4.6 µm pixel, ~1000e- full-well capacity (sounds a bit low?), no word on pixel gain homogeneity/correction https://twitter.com/openspim/status/1395045417503666180

Good point for @AndorTechnology that uses proper 0-100% Y axis on the QE graph. I have an @HamamatsuPhoton V2 though https://twitter.com/openspim/status/653853528914591744

After spectrally-resolved STORM, here comes spectrally-resolved LSM https://twitter.com/openspim/status/639090801742626816

Reminds me of Erik Manders' CLEM strategy (http://www.imaging-git.com/science/light-microscopy/controlled-light-exposure-microscopy-clem) https://twitter.com/openspim/status/634278820569313280