#PA-GFP
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plant-cyclist.bsky.social
📢 Preprint Update 📢

New Title 📰
More Data 📊
New Co-Author: @luiselotterausch.bsky.social 👋

We show simultaneous dual-color #sptPALM in live #Arabidopsis hypocotyl cells using codon-optimized #PA-GFP and #PATagRFP. 🌱🔬

Check it out👉 t1p.de/6cybn

@biorxiv-plants.bsky.social
t1p.de
June 3, 2026 at 1:25 PM
plant-cyclist.bsky.social
Presenting our research to the Bsky Community – Part II! 🌱🔬

Check out our #bioRxiv #preprint on #Dual-Color #sptPALM in #Arabidopsis 🌱! Using #PA-GFP & #PATagRFP, we enable simultaneous tracking of two proteins in living plant cells! 🎨🎉

👉 Read more: tinyurl.com/2vcry74m
Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM
Super-resolution microscopy techniques have revolutionized cell biology by providing insights into the dynamics of single molecules and nanoscale organization within living cells. However, the applica...
tinyurl.com
February 8, 2025 at 1:37 PM
psalmotoxin.bsky.social
Taking advantage of GFP as a marker, we recorded from knee-innervating neurons post-mortem: CFA-induced depolarisation, & ⬇️ rheobase did not occur in Prdm12-AAV 🐁
Prdm12 overexpression prevents CFA-induced neuronal hyperexcitability of knee-innervating afferent DRG neurons Analysis of action potential properties of knee-innervating DRG neurons from female mice injected with Control-AAV or Prdm12-AAV in both knees, and subsequent unilateral CFA injection, comparing neurons from the CFA-injected side (Ipsi) with those innervating the non-injected side (Contra): (A) Representative action potential traces in response to current injection, (B) Rheobase (pA), (C) Resting membrane potential (mV), (D) Action potential amplitude (mV), (E) Representative suprathreshold (2x rheobase) action potential traces, and (F) Number of action potentials generated in neurons stimulated at suprathreshold stimulation (2x rheobase). Data were collected from 4 female mice per group over 3 independent experiments involving AAV injections followed by DRG neuronal dissociation. Sample sizes: Control-AAV n=4; Prdm12-AAV n=5. Statistical analysis: One-way ANOVA followed by Tukey's multiple comparison test. Data are presented as mean ± SEM. Abbreviations: Ipsi = Ipsilateral (CFA-injected side); Contra = Contralateral (non-injected side)
October 16, 2025 at 6:29 AM
vesiclesmasher.bsky.social
A smaller protein, GPI-GFP, doesn’t move much under 2 Pascale of shear stress. But we can convert it from immobile to mobile under 2 Pa by allowing a GFP antibody to bind, increasing its size and the hydrodynamic force applied to it (Sreeja measured the force).
August 30, 2025 at 3:17 AM
christletx.bsky.social
pa-GFP article (Patterson Science 2002): https://science.sciencemag.org/content/297/5588/1873
January 2, 2025 at 4:02 PM
plosbiology.org
How do #neurons target multiple postsynaptic partners? This study shows that #Drosophila #KenyonCells flexibly distribute their synaptic scaffolds across brain sub-compartments; this #plasticity is regulated by #octopaminergic signaling & physiological stress @plosbiology.org 🧪 plos.io/4o5XsPN
Top left: Schematic of split-GFP tagging system. GFP11 was inserted prior to the stop codon of brp, while GFP1–10 was expressed in specific cell types using the GAL4-UAS system. GFP11 and GFP1–10 reconstitute to emit fluorescence. Top right: Long-term live imaging of Brp::rGFP (green) in a growing motor neuron (magenta, CD4::tdTomato) from 48 to 96 hAPF. OK371-GAL4 was used to express GFP1–10 and CD4::tdTomato. White boxes indicate zoomed-in areas shown in the lower panels. Scale bars: upper panels, 10 μm; lower panels, 5 μm. Bottom: Internal states adjust the Brp compartmental heterogeneity through octopamine. Graphical summary of the working model. Localized innervation of OANs onto γ KCs regulates the Brp accumulation and forms the Brp compartmental heterogeneity via Octβ2R and cAMP pathway. Nutritional states may modulate OAN activity, thereby adjusting the Brp heterogeneity.
October 27, 2025 at 9:03 AM
plosbiology.org
How do #neurons target multiple postsynaptic partners? This study shows that #Drosophila #KenyonCells flexibly distribute their synaptic scaffolds across brain sub-compartments; this #plasticity is regulated by #octopaminergic signaling & physiological stress @plosbiology.org 🧪 plos.io/4o5XsPN
Top left: Schematic of split-GFP tagging system. GFP11 was inserted prior to the stop codon of brp, while GFP1–10 was expressed in specific cell types using the GAL4-UAS system. GFP11 and GFP1–10 reconstitute to emit fluorescence. Top right: Long-term live imaging of Brp::rGFP (green) in a growing motor neuron (magenta, CD4::tdTomato) from 48 to 96 hAPF. OK371-GAL4 was used to express GFP1–10 and CD4::tdTomato. White boxes indicate zoomed-in areas shown in the lower panels. Scale bars: upper panels, 10 μm; lower panels, 5 μm. Bottom: Internal states adjust the Brp compartmental heterogeneity through octopamine. Graphical summary of the working model. Localized innervation of OANs onto γ KCs regulates the Brp accumulation and forms the Brp compartmental heterogeneity via Octβ2R and cAMP pathway. Nutritional states may modulate OAN activity, thereby adjusting the Brp heterogeneity.
October 24, 2025 at 4:25 PM
plosbiology.org
How do #neurons target multiple postsynaptic partners? This study shows that #Drosophila #KenyonCells flexibly distribute their synaptic scaffolds across brain sub-compartments; this #plasticity is regulated by #octopaminergic signaling & physiological stress @plosbiology.org 🧪 plos.io/4o5XsPN
Top left: Schematic of split-GFP tagging system. GFP11 was inserted prior to the stop codon of brp, while GFP1–10 was expressed in specific cell types using the GAL4-UAS system. GFP11 and GFP1–10 reconstitute to emit fluorescence. Top right: Long-term live imaging of Brp::rGFP (green) in a growing motor neuron (magenta, CD4::tdTomato) from 48 to 96 hAPF. OK371-GAL4 was used to express GFP1–10 and CD4::tdTomato. White boxes indicate zoomed-in areas shown in the lower panels. Scale bars: upper panels, 10 μm; lower panels, 5 μm. Bottom: Internal states adjust the Brp compartmental heterogeneity through octopamine. Graphical summary of the working model. Localized innervation of OANs onto γ KCs regulates the Brp accumulation and forms the Brp compartmental heterogeneity via Octβ2R and cAMP pathway. Nutritional states may modulate OAN activity, thereby adjusting the Brp heterogeneity.
October 24, 2025 at 12:52 PM