#sptPALM
LightNews LightNews
luiselotterausch.bsky.social
NEW RESULTS 🔬📢

Here's the latest version of our preprint, now with a whole new dataset on the role of BAK1 in nanoscale regulation of rapid hormonal responses.
Check out this interdisciplinary project using #AFM , #CarboTag based FLIM and #sptPALM superresolution!☺️

www.biorxiv.org/content/10.6...
Distinct nanoscale dynamics of growth receptor complexes link hormone perception to rapid cell wall remodeling
Cell elongation is a fundamental process allowing plants to change size and shape, a process governed by several growth promoting hormones. While hormones such as brassinosteroids (BRs) and phytosulfo...
www.biorxiv.org
October 27, 2026 at 11:46 AM
luiselotterausch.bsky.social
New Preprint!

We use #AFM and CarboTag-based #FLIM to describe rapid cell wall mechanisms of Phytosulfokines & Brassinosteroids and investigate how these processes are initiated on the nanoscale by using #sptPALM!

PS: This is also the main story of my PhD project😊✌🏻

www.biorxiv.org/content/10.6...
Distinct nanoscale dynamics of growth receptor complexes link hormone perception to rapid cell wall remodeling
Cell elongation is a fundamental process allowing plants to change size and shape, a process governed by several growth promoting hormones. While hormones such as brassinosteroids (BRs) and phytosulfo...
www.biorxiv.org
September 11, 2026 at 8:27 AM
hohlbeinlab.bsky.social
(LinkedIn cross post) 0.11.3 of #webSMLM released. Since 0.11.0 we added: headless spectral SMLM runs, *.ND2 loading, single particle tracking (sptPALM, Nile red PAINT, etc), light (see below), dark or high contrast mode; *.svg export of plots, change to MIT license,... (1/2)
User interface of webSMLM, center window histogram of diffusion coefficients of Nile Red diffusing in bacterial cell wall, right window bacterial cell outlines of L. actis stained with Nile Red.
August 21, 2026 at 7:40 AM
hohlbeinlab.bsky.social
Opus Magnum by @loreoliv.bsky.social on the role of DnaA in bacterial DNA replication initiation building on fancy combinations of #sptPALM, proteomics, flow cytometry, genetics, and other techniques. Great collab with the Staals lab and many others. Long 🧵👇
August 22, 2025 at 9:03 AM
hohlbeinlab.bsky.social
#Python, #Seaborn & #Pandas is growing on me. Some synthetic data to plot average diffusion coefficients etc of tracks in individual bacterial cells (independent repeats of different conditions). #sptPALM is hard. Getting there @jmakela.bsky.social + @loreoliv.bsky.social
Four different figures showcasing strip plots, scatter plots, and box plots of synthetic #sptPALM data
December 7, 2024 at 8:21 PM
hohlbeinlab.bsky.social
This week will hopefully bring the answer to the very important question whether mMaple3 is more photostable than PAmCherry2 in E. coli #sptPALM. Can't wait. @loreoliv.bsky.social
November 18, 2024 at 2:48 PM
hohlbeinlab.bsky.social
Fast dynamics in #sptPALM? Log-scale to the rescue. Three state model for Cas9 diffusion in .
October 24, 2024 at 10:19 AM
hohlbeinlab.bsky.social
Playing with a GUI for #sptPALM simulations and #Python #Seaborn
graphical user interface for a simulation nice plots
October 23, 2024 at 9:09 AM
hohlbeinlab.bsky.social
Some colleagues bought some #pioreactors for our next set of #sptPALM experiments. Stable for 100+ hours and you can exchange as much buffer as you like after reaching threshold (saw tooth). Excellent value for money it looks like.
Reading of a turbidostat showing stability over 100 hours. 8+ turbidostats in a row.
October 21, 2024 at 8:07 AM
hohlbeinlab.bsky.social
New preprint spear headed by the amazing #LorenzoOlivi: "The Escherichia coli replication initiator DnaA is titrated on the chromosome" using, among many other techniques, #sptPALM to determine growth dependent mobilities of DnaA diffusing in live cells. doi.org/10.1101/2024.10.07.617004 1/n
The Escherichia coli replication initiator DnaA is titrated on the chromosome
DNA replication initiation is orchestrated in many prokaryotes by the replication initiator DnaA. Two models for regulation of DnaA activity in Escherichia coli have been proposed: the switch between ...
doi.org
October 12, 2024 at 1:37 PM
luiselotterausch.bsky.social
Check out our updated preprint where we introduce dual-color #sptpalm #superresolution microscopy 🔬 for tracking membrane proteins in live plant cells 🌱

Lead by @plant-cyclist.bsky.social at @zmbp-tuebingen.bsky.social
plant-cyclist.bsky.social Leander Rohr, PhD @plant-cyclist.bsky.social · Jun 3
📢 Preprint Update 📢

New Title 📰
More Data 📊
New Co-Author: @luiselotterausch.bsky.social 👋

We show simultaneous dual-color #sptPALM in live #Arabidopsis hypocotyl cells using codon-optimized #PA-GFP and #PATagRFP. 🌱🔬

Check it out👉 t1p.de/6cybn

@biorxiv-plants.bsky.social
t1p.de
June 3, 2026 at 2:03 PM
plant-cyclist.bsky.social
📢 Preprint Update 📢

New Title 📰
More Data 📊
New Co-Author: @luiselotterausch.bsky.social 👋

We show simultaneous dual-color #sptPALM in live #Arabidopsis hypocotyl cells using codon-optimized #PA-GFP and #PATagRFP. 🌱🔬

Check it out👉 t1p.de/6cybn

@biorxiv-plants.bsky.social
t1p.de
June 3, 2026 at 1:25 PM
plant-cyclist.bsky.social
With Sven zur Oven-Krockhaus, @luiselotterausch.bsky.social & Klaus Harter we wrote an #ExpertView on #sptPALM in #plants 🌱 (focus: #plasmamembrane).
✔️ Our goal: help you get started with this powerful method 🚀
❓ Curious? We’ll be happy to get you ready!
October 2, 2025 at 8:35 AM
plant-cyclist.bsky.social
Presenting our research to the Bsky Community – Part II! 🌱🔬

Check out our #bioRxiv #preprint on #Dual-Color #sptPALM in #Arabidopsis 🌱! Using #PA-GFP & #PATagRFP, we enable simultaneous tracking of two proteins in living plant cells! 🎨🎉

👉 Read more: tinyurl.com/2vcry74m
Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM
Super-resolution microscopy techniques have revolutionized cell biology by providing insights into the dynamics of single molecules and nanoscale organization within living cells. However, the applica...
tinyurl.com
February 8, 2025 at 1:37 PM
loreoliv.bsky.social
The best resource for #microscopy is among us on BlueSky! Savior of my #sptPALM designs
fpbase.org FPbase @fpbase.org · Nov 18
Hello friends! We’re here to celebrate and share all things fluorescent. Tag us as you share findings related to the use or publication of FPs and other probes
November 18, 2024 at 9:01 PM
hannah-web.bsky.social
Excited to share our #bioRxiv #preprint on HIR2, RLKs and membrane organisation in #Arabidopsis. We used many exciting techniques like #sptPALM, #AlphaFold and #BN-PAGE 🌱
biorxiv-plants.bsky.social bioRxiv Plant Bio @biorxiv-plants.bsky.social · Apr 14
Arabidopsis HYPERSENSITIVE INDUCED REACTION 2 affects plasma membrane receptor pathways and organization https://www.biorxiv.org/content/10.1101/2025.04.11.648320v1
April 15, 2025 at 5:24 AM
christletx.bsky.social
PhD defense with an upgraded setup! Listening to @piponunesv from @GiannoneLab presenting his nice work looking at single adhesion protein by sptPALM during stretch. Feat @ManuelTHERY @PlanarPolarityT @S_LevequeFort C. Grashoff and P. Nassoy
January 2, 2025 at 2:56 PM
christletx.bsky.social
Also from prev link: inferenceMAP generates diffusion landscapes from sptPALM data (pdf link http://arxiv.org/pdf/1504.00525.pdf)
December 26, 2024 at 6:16 PM
christletx.bsky.social
XD switches to sptPALM of transcription factors: tracking and using angle correlation between trajectory steps to detect constrains #iphria
December 26, 2024 at 4:52 PM
jxbotany.bsky.social
🔬 EXPERT VIEW 🔬

Oven-Krockhaus et al. introduce single-particle tracking photoactivated localization microscopy for studying plasma membrane proteins in plants, outlining key applications and practical guidance on instrumentation, probes, and analysis.

🔗 doi.org/10.1093/jxb/...
#PlantScience 🧪
Fig. 1 (shortened, full legend in paper): Workflow of single-particle tracking photoactivated localization microscopy (sptPALM) analysis. (A) Localization: single molecule spots are cut out of the raw data image stack, fitted with two-dimensional Gaussian functions and the results (most importantly, their coordinates) stored as lists. (B) Tracking: single-molecule positions are grouped to tracks, based on their relative positions in subsequent frames. (C) Mean square displacement (MSD) analysis: the average squared distance the molecules have traveled in certain time intervals (top left) can be plotted in MSD curves (bottom), showing a straight line for free Brownian motion (dark gray), for which the slope is directly proportional to the diffusion coefficient. Other motion types like superdiffusive (red) or subdiffusive (yellow) show different curve shapes (inset: corresponding single-molecule example tracks).
December 28, 2025 at 9:20 AM
somssich.bsky.social
📜 Simultaneous and Dynamic Super‐Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual‐color sptPALM

🧑‍🔬 Leander Rohr, Sven zur Oven‐Krockhaus, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantMicroscopy #Microscopy #SuperResMicroscopy
Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
www.biorxiv.org
March 18, 2024 at 8:10 AM
biorxivpreprint.bsky.social
Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM https://www.biorxiv.org/content/10.1101/2024.03.13.584811v1
Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM https://www.biorxiv.org/content/10.1101/2024.03.13.584811v1
Super-resolution microscopy techniques have revolutionized cell biology by providing insights into t
www.biorxiv.org
March 16, 2024 at 1:02 AM
plantubiquitin.bsky.social
ALFA tag even allowed us to explore trifunctional fluorescence complementation (TriFC), targeted protein degradation and super-resolution sptPALM imaging for the notoriously difficult to work with Arabidopsis IRT1 root iron transporter.
March 20, 2025 at 2:05 PM
biorxiv-plants.bsky.social
Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM https://www.biorxiv.org/content/10.1101/2024.03.13.584811v1
Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM https://www.biorxiv.org/content/10.1101/2024.03.13.584811v1
Super-resolution microscopy techniques have revolutionized cell biology by providing insights into t
www.biorxiv.org
March 16, 2024 at 1:02 AM