Out in @narjournal.bsky.social! DNA defenses can destroy incoming plasmids, leaving cells vulnerable to repeated conjugation and cell death (lethal zygosis).
With @therubinlab.bsky.social @leosong.bsky.social
doi.org/10.1093/nar/...
We made a short video explainer 👇
#plasmids #microsky
With @therubinlab.bsky.social @leosong.bsky.social
doi.org/10.1093/nar/...
We made a short video explainer 👇
#plasmids #microsky
October 29, 2026 at 3:55 PM
Out in @narjournal.bsky.social! DNA defenses can destroy incoming plasmids, leaving cells vulnerable to repeated conjugation and cell death (lethal zygosis).
With @therubinlab.bsky.social @leosong.bsky.social
doi.org/10.1093/nar/...
We made a short video explainer 👇
#plasmids #microsky
With @therubinlab.bsky.social @leosong.bsky.social
doi.org/10.1093/nar/...
We made a short video explainer 👇
#plasmids #microsky
IGI's Ben Rubin (Rubin Lab), Jonathan Martinson, and Leo Song reveal that plasmids can actively kill bacteria that resist taking them in, driving their own spread throughout bacterial populations. Learn more in this short explainer: https://www.youtube.com/watch?v=4vzOoFFj3cE
#microbiology
#microbiology
“Join or Die”: DNA Plasmids Kill Bacteria that Resist Them
“Join or Die”: DNA Plasmids Kill Bacteria that Resist Them - Innovative Genomics Institute (IGI)
innovativegenomics.org
November 2, 2026 at 5:40 PM
IGI's Ben Rubin (Rubin Lab), Jonathan Martinson, and Leo Song reveal that plasmids can actively kill bacteria that resist taking them in, driving their own spread throughout bacterial populations. Learn more in this short explainer: https://www.youtube.com/watch?v=4vzOoFFj3cE
#microbiology
#microbiology
Out now in @natcomms.nature.com: We found a plasmid in a preterm infant’s gut microbiome that’s 1.58 million base pairs long, about 30X the size of a typical E. coli plasmid. Then we found its relatives in hundreds of microbiomes worldwide. Read on to learn more about the elusive pMEGAs! 🧵
Identification and genetic characterization of megabase-scale plasmids of Enterobacteriaceae - Nature Communications
Here, the authors report the identification and characterization of large, up to 1.58 million base-pair-long plasmids in gut microbiomes worldwide, showing that while most genes remain uncharacterized...
www.nature.com
October 27, 2026 at 2:52 PM
Out now in @natcomms.nature.com: We found a plasmid in a preterm infant’s gut microbiome that’s 1.58 million base pairs long, about 30X the size of a typical E. coli plasmid. Then we found its relatives in hundreds of microbiomes worldwide. Read on to learn more about the elusive pMEGAs! 🧵
New Rubin Lab paper! 🎉 Amazing work by @jnvmartinson.bsky.social and @leosong.bsky.social.
🔗 doi.org/10.1093/nar/...
🔗 doi.org/10.1093/nar/...
October 29, 2026 at 4:17 PM
New Rubin Lab paper! 🎉 Amazing work by @jnvmartinson.bsky.social and @leosong.bsky.social.
🔗 doi.org/10.1093/nar/...
🔗 doi.org/10.1093/nar/...
🎉New paper in FEMS Microbiology Ecology!
Plasmid–plasmid interactions reshape extracellular vesicle cargo and antimicrobial resistance gene transfer potential
We (led by F. Tskhay) show that plasmid coexistence reverses which plasmid dominates EV cargo!
🔗 doi.org/10.1093/fems...
Plasmid–plasmid interactions reshape extracellular vesicle cargo and antimicrobial resistance gene transfer potential
We (led by F. Tskhay) show that plasmid coexistence reverses which plasmid dominates EV cargo!
🔗 doi.org/10.1093/fems...
Plasmid–plasmid interactions reshape extracellular vesicle cargo and antimicrobial resistance gene transfer potential
Abstract. Plasmids are major drivers of bacterial horizontal gene transfer, including transfer of antimicrobial resistance. Plasmid dissemination extends b
doi.org
November 2, 2026 at 10:50 AM
🎉New paper in FEMS Microbiology Ecology!
Plasmid–plasmid interactions reshape extracellular vesicle cargo and antimicrobial resistance gene transfer potential
We (led by F. Tskhay) show that plasmid coexistence reverses which plasmid dominates EV cargo!
🔗 doi.org/10.1093/fems...
Plasmid–plasmid interactions reshape extracellular vesicle cargo and antimicrobial resistance gene transfer potential
We (led by F. Tskhay) show that plasmid coexistence reverses which plasmid dominates EV cargo!
🔗 doi.org/10.1093/fems...
Of the two plasmids within their host, the more chromosome-like symbiosis plasmid showed extensive regulatory and metabolic interdependence with the chromosome and significant fitness contribution in-planta. This is cool for flagellar genes and in-planta association of motility with the plasmids.
November 1, 2026 at 11:11 AM
Of the two plasmids within their host, the more chromosome-like symbiosis plasmid showed extensive regulatory and metabolic interdependence with the chromosome and significant fitness contribution in-planta. This is cool for flagellar genes and in-planta association of motility with the plasmids.
A plasmid-encoded H-NS protein selectively binds its own plasmid | mSystems
A plasmid-encoded H-NS protein selectively binds its own plasmid | mSystems
Many bacteria carry conjugative plasmids, mobile DNA molecules that spread traits
such as antibiotic resistance. Some conjugative plasmids encode proteins related to
the bacterial DNA-binding protein H-NS. We show that an H-NS-like protein from the
IncX4 plasmid pCROD2 binds almost exclusively to the plasmid from which it originates,
while largely ignoring the host chromosome. Our findings reveal a previously unrecognized
mechanism that allows plasmids to regulate their own genes with high specificity while
minimizing interference with host gene expression.
journals.asm.org
October 28, 2026 at 1:57 AM
A plasmid-encoded H-NS protein selectively binds its own plasmid | mSystems
PlasmidNL lets users contextualize their own plasmid data against a Dutch national surveillance collection of 4,596 plasmids from carbapenemase-producing organisms.
November 2, 2026 at 1:03 PM
PlasmidNL lets users contextualize their own plasmid data against a Dutch national surveillance collection of 4,596 plasmids from carbapenemase-producing organisms.
Are peptides plasmids?
October 29, 2026 at 4:56 AM
Are peptides plasmids?
Estou em 58% de "Bioshock: Rapture" - John Shirley
★ meu deus os drogados por plasmids já estão tacando o terror e como ver a evolução disso é horrível como a humanidade vai sendo tirada deles
(eu tenho muito medo deles nos jogos)
🟠 aflita | 🔴 amedrontada
💜📚 → maratona.social/att/cmvwxgvh...
★ meu deus os drogados por plasmids já estão tacando o terror e como ver a evolução disso é horrível como a humanidade vai sendo tirada deles
(eu tenho muito medo deles nos jogos)
🟠 aflita | 🔴 amedrontada
💜📚 → maratona.social/att/cmvwxgvh...
Noma Dumezweni
ALT: Noma Dumezweni
static.klipy.com
October 31, 2026 at 9:44 PM
Estou em 58% de "Bioshock: Rapture" - John Shirley
★ meu deus os drogados por plasmids já estão tacando o terror e como ver a evolução disso é horrível como a humanidade vai sendo tirada deles
(eu tenho muito medo deles nos jogos)
🟠 aflita | 🔴 amedrontada
💜📚 → maratona.social/att/cmvwxgvh...
★ meu deus os drogados por plasmids já estão tacando o terror e como ver a evolução disso é horrível como a humanidade vai sendo tirada deles
(eu tenho muito medo deles nos jogos)
🟠 aflita | 🔴 amedrontada
💜📚 → maratona.social/att/cmvwxgvh...
The Tribolium Brainbow plasmids are available here: www.addgene.org/browse/artic...
October 27, 2026 at 8:54 PM
The Tribolium Brainbow plasmids are available here: www.addgene.org/browse/artic...
Many thanks to the entire @banfieldlab.bsky.social lab, the team at @innovativegenomics.bsky.social, Dr. Michael Morowitz, and our collaborators across the US, Switzerland, the UK, Finland, Singapore, and Vietnam. www.nature.com/articles/s41...
Identification and genetic characterization of megabase-scale plasmids of Enterobacteriaceae - Nature Communications
Here, the authors report the identification and characterization of large, up to 1.58 million base-pair-long plasmids in gut microbiomes worldwide, showing that while most genes remain uncharacterized...
www.nature.com
October 27, 2026 at 2:52 PM
Many thanks to the entire @banfieldlab.bsky.social lab, the team at @innovativegenomics.bsky.social, Dr. Michael Morowitz, and our collaborators across the US, Switzerland, the UK, Finland, Singapore, and Vietnam. www.nature.com/articles/s41...
Halfway through they finally got to ancap Epcot crack (plasmids)
October 29, 2026 at 12:05 PM
Halfway through they finally got to ancap Epcot crack (plasmids)
Oh pancakes are good. But could I interest you in another P word: Plasmids? Amazing powers and absolutely no downsides, nope, not a one.
Bioshock 1 Inject
ALT: Bioshock 1 Inject
static.klipy.com
October 29, 2026 at 12:29 PM
Oh pancakes are good. But could I interest you in another P word: Plasmids? Amazing powers and absolutely no downsides, nope, not a one.
Bioshock 1 Inject
Alt: Bioshock 1 Injection of plasmids
static.klipy.com
October 29, 2026 at 4:57 AM
Meet JEDI3sub and JEDI3hyp, the latest voltage indicators from François St-Pierre's lab at @bcmhouston.bsky.social. Built for recording of subthreshold voltage dynamics, so you can image deeper and capture more. 🔬⚡
Get the plasmids (or AAV Packaged on Request!) here:
Get the plasmids (or AAV Packaged on Request!) here:
www.addgene.org
Addgene
twp.ai
October 30, 2026 at 5:00 PM
Meet JEDI3sub and JEDI3hyp, the latest voltage indicators from François St-Pierre's lab at @bcmhouston.bsky.social. Built for recording of subthreshold voltage dynamics, so you can image deeper and capture more. 🔬⚡
Get the plasmids (or AAV Packaged on Request!) here:
Get the plasmids (or AAV Packaged on Request!) here:
The Netherlands is transforming the genomic antimicrobial resistance (AMR) surveillance by integrating resistance plasmid analysis using “PlasmidNL” to monitor plasmid outbreaks and spread. AND YOU CAN USE IT TOO!
#AMR #Genomics #surveillance #Nanopore #plasmids #CPE #MRSA #PlasmidOutbreaks #ONT
#AMR #Genomics #surveillance #Nanopore #plasmids #CPE #MRSA #PlasmidOutbreaks #ONT
October 30, 2026 at 6:54 AM
The Netherlands is transforming the genomic antimicrobial resistance (AMR) surveillance by integrating resistance plasmid analysis using “PlasmidNL” to monitor plasmid outbreaks and spread. AND YOU CAN USE IT TOO!
#AMR #Genomics #surveillance #Nanopore #plasmids #CPE #MRSA #PlasmidOutbreaks #ONT
#AMR #Genomics #surveillance #Nanopore #plasmids #CPE #MRSA #PlasmidOutbreaks #ONT
A big thanks to BBSRC, EPSRC, @royalsociety.org and others for funding and support. This is the second of numerous strains (SHARK was our first doi.org/10.1021/acss...) we are looking to release soon to help accelerate all sorts of synthetic and engineering biology!
SHARK: A Specialized Host for Assembling R6K Plasmids
Abstract. R6K plasmids are
commonly used for a wide range of genome engineering
applications due to their ability to support transient delivery of
genetic
doi.org
October 28, 2026 at 11:12 AM
A big thanks to BBSRC, EPSRC, @royalsociety.org and others for funding and support. This is the second of numerous strains (SHARK was our first doi.org/10.1021/acss...) we are looking to release soon to help accelerate all sorts of synthetic and engineering biology!
pondering the fish
[ #flameshadowart #silksong | wardenfly, lavalarga, flea caravan draftbug, maestro, cogfly, beastfly, kai, plasmid, steel seer zi ]
[ #flameshadowart #silksong | wardenfly, lavalarga, flea caravan draftbug, maestro, cogfly, beastfly, kai, plasmid, steel seer zi ]
October 30, 2026 at 1:03 PM
pondering the fish
[ #flameshadowart #silksong | wardenfly, lavalarga, flea caravan draftbug, maestro, cogfly, beastfly, kai, plasmid, steel seer zi ]
[ #flameshadowart #silksong | wardenfly, lavalarga, flea caravan draftbug, maestro, cogfly, beastfly, kai, plasmid, steel seer zi ]
You know, great response. I had completely forgotten these were called Plasmids 😂. That's an awesome and informative answer. 😊
And definitely no downsides, nope. None at all. 😂
And definitely no downsides, nope. None at all. 😂
Is It Meme
ALT: Is It Meme
static.klipy.com
October 29, 2026 at 12:50 PM
You know, great response. I had completely forgotten these were called Plasmids 😂. That's an awesome and informative answer. 😊
And definitely no downsides, nope. None at all. 😂
And definitely no downsides, nope. None at all. 😂
Searching millions of public sequencing datasets revealed diverse pMEGA relatives in infants, adults, chickens, pandas, and hospital sinks. Given their size, these plasmids were often highly fragmented in assemblies, which may explain why they were missed. #metagenomics
October 27, 2026 at 2:52 PM
Searching millions of public sequencing datasets revealed diverse pMEGA relatives in infants, adults, chickens, pandas, and hospital sinks. Given their size, these plasmids were often highly fragmented in assemblies, which may explain why they were missed. #metagenomics
Type IV-Dominated Plasmid-Encoded CRISPR-Cas Systems Are Enriched in Multireplicon and Multi-Stressor Resistance Plasmids Across the Global Plasmidome pubmed.ncbi.nlm.nih.gov/42947566/
October 30, 2026 at 3:57 AM
Type IV-Dominated Plasmid-Encoded CRISPR-Cas Systems Are Enriched in Multireplicon and Multi-Stressor Resistance Plasmids Across the Global Plasmidome pubmed.ncbi.nlm.nih.gov/42947566/
Earlier, they showed the domestication of accessory plasmids coincided with speciation of plant-associated Pantoea sp. Phylogenetic analysis identified gene gain, differential loss, synchronized replication of plasmids:chromosome, and placed them on a horizontal-vertical inheritance transition.
November 1, 2026 at 11:10 AM
Earlier, they showed the domestication of accessory plasmids coincided with speciation of plant-associated Pantoea sp. Phylogenetic analysis identified gene gain, differential loss, synchronized replication of plasmids:chromosome, and placed them on a horizontal-vertical inheritance transition.
Register: forms.gle/H2sGBBcoy1xm...
11 days to go.
Is your plasmid the one you designed? Prof. Boas Pucker (PuckerLab, Uni Bonn) shows how #NanoPlasmiQC checks whole plasmids with ONT long reads in a day.
SynBio4ALL Africa Monthly Webinar
Fri 13 Nov, 8 pm EAT (5 pm GMT). Free.
11 days to go.
Is your plasmid the one you designed? Prof. Boas Pucker (PuckerLab, Uni Bonn) shows how #NanoPlasmiQC checks whole plasmids with ONT long reads in a day.
SynBio4ALL Africa Monthly Webinar
Fri 13 Nov, 8 pm EAT (5 pm GMT). Free.
November 2, 2026 at 2:29 PM
Register: forms.gle/H2sGBBcoy1xm...
11 days to go.
Is your plasmid the one you designed? Prof. Boas Pucker (PuckerLab, Uni Bonn) shows how #NanoPlasmiQC checks whole plasmids with ONT long reads in a day.
SynBio4ALL Africa Monthly Webinar
Fri 13 Nov, 8 pm EAT (5 pm GMT). Free.
11 days to go.
Is your plasmid the one you designed? Prof. Boas Pucker (PuckerLab, Uni Bonn) shows how #NanoPlasmiQC checks whole plasmids with ONT long reads in a day.
SynBio4ALL Africa Monthly Webinar
Fri 13 Nov, 8 pm EAT (5 pm GMT). Free.