#ProximityLabeling
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mick-lab.bsky.social
Proud to share our work spear-headed by PhD student Tommy Sroka with major contributions by #xenopus expert Kerstin Feistel.

Our iAPEX #ProximityLabeling method for #MassSpec based subcellular #proteomics works by locally generating H2O2 using a D-amino acid oxidase that activates APEX2 in situ.
iAPEX: Improved APEX-based proximity labeling for subcellular proteomics using an enzymatic reaction cascade
Ascorbate peroxidase (APEX) is a versatile labeling enzyme used for live-cell proteomics at high spatial and temporal resolution. However, toxicity of its substrate hydrogen peroxide and background la...
www.biorxiv.org
January 11, 2025 at 7:51 PM
piamadeleine.bsky.social
Day 3 of #GfV2025 in Hamburg ☀️!
I am looking forward to presenting my PhD project today at Poster #411 and to great discussions and interactions!

#Influenza #HPAIV #PKR #Proximitylabeling #TurboID
March 6, 2025 at 9:57 AM
stephanhacker2.bsky.social
Great talk by Xinyuan Fan at #ISCPDD. He talked about his group’s exciting work on using #photocatalysis to profile the proteins and the RNA in specific compartments in the cell on the example of mitochondria, Golgi, ER and lysosome.
doi.org/10.1038/s415...
#ChemBio #ChemSky #ProximityLabeling
Photocatalytic labelling-enabled subcellular-resolved RNA profiling and synchronous multi-omics investigation
Nature Chemistry - Profiling subcellular multi-omics with spatiotemporal resolution remains a key challenge in cell biology. Now an orthogonal photocatalytic labelling strategy has been developed...
doi.org
November 25, 2025 at 8:00 AM
plosbiology.org
Despite advances in #ProximityLabeling tools, we can't capture intermediary proteins that bridge two associated non-interacting proteins. STUPPIT is a novel method to label intermediary proteins between two non-interacting partners in signaling pathways @plosbiology.org 🧪 plos.io/4ab6YwQ
This artwork was inspired by the ancient Chinese legend of "The Cowherd and the Weaver Girl," where two star-crossed lovers are reunited once a year by a bridge of magpies. Here, the Cowherd and Weaver Girl represent two non-interacting proteins (e.g., actin and YAP1), separated by the Milky Way—symbolizing the complex cellular environment. The magpie bridge, formed by STUPPIT components with Pup(E) as the body and Split-TurboID fragments as glowing wings, enables their connection. Soaring lanterns released from the bridge represent biotin-labeled proximal proteins (e.g., AMOT), illuminating the functional interactome between the once-distant proteins. Xie et al. demonstrate how the STUPPIT system acts as a molecular "magpie bridge," enabling proximity labeling and revealing functional interactomes between proteins that do not directly bind—just as the magpie bridge reunites the star-crossed lovers. Artwork by graduate student Xin Li.
November 26, 2025 at 5:35 PM
plosbiology.org
Despite advances in #ProximityLabeling tools, we can't capture intermediary proteins that bridge two associated non-interacting proteins. STUPPIT is a novel method to label intermediary proteins between two non-interacting partners in signaling pathways @plosbiology.org 🧪 plos.io/4ab6YwQ
This artwork was inspired by the ancient Chinese legend of "The Cowherd and the Weaver Girl," where two star-crossed lovers are reunited once a year by a bridge of magpies. Here, the Cowherd and Weaver Girl represent two non-interacting proteins (e.g., actin and YAP1), separated by the Milky Way—symbolizing the complex cellular environment. The magpie bridge, formed by STUPPIT components with Pup(E) as the body and Split-TurboID fragments as glowing wings, enables their connection. Soaring lanterns released from the bridge represent biotin-labeled proximal proteins (e.g., AMOT), illuminating the functional interactome between the once-distant proteins. Xie et al. demonstrate how the STUPPIT system acts as a molecular "magpie bridge," enabling proximity labeling and revealing functional interactomes between proteins that do not directly bind—just as the magpie bridge reunites the star-crossed lovers. Artwork by graduate student Xin Li.
November 26, 2025 at 1:58 PM
plosbiology.org
Despite advances in #ProximityLabeling tools, we can't capture intermediary proteins that bridge two associated non-interacting proteins. STUPPIT is a novel method to label intermediary proteins between two non-interacting partners in signaling pathways @plosbiology.org 🧪 plos.io/4ab6YwQ
This artwork was inspired by the ancient Chinese legend of "The Cowherd and the Weaver Girl," where two star-crossed lovers are reunited once a year by a bridge of magpies. Here, the Cowherd and Weaver Girl represent two non-interacting proteins (e.g., actin and YAP1), separated by the Milky Way—symbolizing the complex cellular environment. The magpie bridge, formed by STUPPIT components with Pup(E) as the body and Split-TurboID fragments as glowing wings, enables their connection. Soaring lanterns released from the bridge represent biotin-labeled proximal proteins (e.g., AMOT), illuminating the functional interactome between the once-distant proteins. Xie et al. demonstrate how the STUPPIT system acts as a molecular "magpie bridge," enabling proximity labeling and revealing functional interactomes between proteins that do not directly bind—just as the magpie bridge reunites the star-crossed lovers. Artwork by graduate student Xin Li.
November 27, 2025 at 8:55 AM
febsj.bsky.social
☕ For a perfect weekend read, explore our Research Highlights for a glimpse into the brilliant work in Issue 8, featured by our Editorial Office.

Discover the #ResearchHighlights, here ➡️ buff.ly/0hKsR7s

#proximitylabeling #antifreeze #autophagy #Catel–Manzkesyndrome #chronickidneydisease
May 1, 2026 at 9:00 AM