Absolute Quantification of Lysosomal Proteins by Multiple Reaction Monitoring Mass Spectrometry and QconCAT Protein Standards www.biorxiv.org/cont...
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#proteomics #prot-preprint
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#proteomics #prot-preprint
January 11, 2025 at 4:20 PM
Absolute Quantification of Lysosomal Proteins by Multiple Reaction Monitoring Mass Spectrometry and QconCAT Protein Standards www.biorxiv.org/cont...
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#proteomics #prot-preprint
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#proteomics #prot-preprint
5 years ago: Reichermeier et al., present a proteomics platform (PIKES), enabling them to study the dynamics of interchangeable multiprotein complexes by combining the #QconCAT technology with chemical freezing. pubmed.ncbi.nlm.nih.gov/31973889/.
#proteomics #massspectrometry #quantitativeproteomics
#proteomics #massspectrometry #quantitativeproteomics
March 3, 2025 at 11:21 AM
5 years ago: Reichermeier et al., present a proteomics platform (PIKES), enabling them to study the dynamics of interchangeable multiprotein complexes by combining the #QconCAT technology with chemical freezing. pubmed.ncbi.nlm.nih.gov/31973889/.
#proteomics #massspectrometry #quantitativeproteomics
#proteomics #massspectrometry #quantitativeproteomics
Pfft, this is easy! you just need to encode the Epstein list using peptides in a QconCAT like protein . Express and analyze using LCMS and then put the results in your jupyter QC notebooks. Easy peasy . Oops was I not supposed to say that?
July 17, 2025 at 7:59 PM
Pfft, this is easy! you just need to encode the Epstein list using peptides in a QconCAT like protein . Express and analyze using LCMS and then put the results in your jupyter QC notebooks. Easy peasy . Oops was I not supposed to say that?
(BioRxiv All) Absolute Quantification of Lysosomal Proteins by Multiple Reaction Monitoring Mass Spectrometry and QconCAT Protein Standards: Lysosomes are membrane-enclosed organelles that play a crucial role in the degradation of intra- and extracellular… http://dlvr.it/THH8D6 #BioRxiv #MassSpecRSS
January 10, 2025 at 12:04 PM
(BioRxiv All) Absolute Quantification of Lysosomal Proteins by Multiple Reaction Monitoring Mass Spectrometry and QconCAT Protein Standards: Lysosomes are membrane-enclosed organelles that play a crucial role in the degradation of intra- and extracellular… http://dlvr.it/THH8D6 #BioRxiv #MassSpecRSS
Our fourth quarterly Newsletter is officially online. As usual, we summarize research publications employing our #QconCAT technology. Have a look: www.polyquant.de/_files/ugd/3.... And watch out for 2025 as we will celebrate the 20th birthday of QconCATs with special editions, promotions etc.🎂🎉🥳
www.polyquant.de
December 18, 2024 at 11:53 AM
Our fourth quarterly Newsletter is officially online. As usual, we summarize research publications employing our #QconCAT technology. Have a look: www.polyquant.de/_files/ugd/3.... And watch out for 2025 as we will celebrate the 20th birthday of QconCATs with special editions, promotions etc.🎂🎉🥳
We combined this and the QConCat concept to create a plasmid that was cut open with a 3 base pair overlap using AdeI.
We encoded the peptide sequence of interest in DNA with a 3 base pair overlap on each end that matched the overhang in the plasmid, typically a lysine.
We encoded the peptide sequence of interest in DNA with a 3 base pair overlap on each end that matched the overhang in the plasmid, typically a lysine.
November 16, 2024 at 12:21 AM
We combined this and the QConCat concept to create a plasmid that was cut open with a 3 base pair overlap using AdeI.
We encoded the peptide sequence of interest in DNA with a 3 base pair overlap on each end that matched the overhang in the plasmid, typically a lysine.
We encoded the peptide sequence of interest in DNA with a 3 base pair overlap on each end that matched the overhang in the plasmid, typically a lysine.
Myself and Hamid Mirzaei experimented with QConCat constructs but we struggled with the differences in response from each peptide that pushed the dynamic range of the MS systems at the time.
November 16, 2024 at 12:21 AM
Myself and Hamid Mirzaei experimented with QConCat constructs but we struggled with the differences in response from each peptide that pushed the dynamic range of the MS systems at the time.
Question for #teammassspec here. Are internal standard peptides like AQUA or QConCat still in use commonly? I haven’t worked in this space for a number of years but worked on a project that never saw the light of day that I may just open source the concept here.
November 15, 2024 at 11:35 PM
Question for #teammassspec here. Are internal standard peptides like AQUA or QConCat still in use commonly? I haven’t worked in this space for a number of years but worked on a project that never saw the light of day that I may just open source the concept here.
📣 💡 New publication employing our #QconCAT technology: 𝗙𝗚𝗙𝟭 𝗮𝗺𝗲𝗹𝗶𝗼𝗿𝗮𝘁𝗲𝘀 𝗵𝗲𝗽𝗮𝘁𝗶𝗰 𝘀𝘁𝗲𝗮𝘁𝗼𝘀𝗶𝘀 𝘁𝗵𝗿𝗼𝘂𝗴𝗵 𝗮𝗰𝘂𝘁𝗲 𝗮𝗰𝘁𝗶𝘃𝗮𝘁𝗶𝗼𝗻 𝗼𝗳 𝘁𝗵𝗲 𝘂𝗻𝗳𝗼𝗹𝗱𝗲𝗱 𝗽𝗿𝗼𝘁𝗲𝗶𝗻 𝗿𝗲𝘀𝗽𝗼𝗻𝘀𝗲 𝗮𝗻𝗱 𝗩𝗟𝗗𝗟 𝗽𝗿𝗼𝗱𝘂𝗰𝘁𝗶𝗼𝗻
pubmed.ncbi.nlm.nih.gov/41541501/
pubmed.ncbi.nlm.nih.gov/41541501/
February 5, 2026 at 10:19 AM
📣 💡 New publication employing our #QconCAT technology: 𝗙𝗚𝗙𝟭 𝗮𝗺𝗲𝗹𝗶𝗼𝗿𝗮𝘁𝗲𝘀 𝗵𝗲𝗽𝗮𝘁𝗶𝗰 𝘀𝘁𝗲𝗮𝘁𝗼𝘀𝗶𝘀 𝘁𝗵𝗿𝗼𝘂𝗴𝗵 𝗮𝗰𝘂𝘁𝗲 𝗮𝗰𝘁𝗶𝘃𝗮𝘁𝗶𝗼𝗻 𝗼𝗳 𝘁𝗵𝗲 𝘂𝗻𝗳𝗼𝗹𝗱𝗲𝗱 𝗽𝗿𝗼𝘁𝗲𝗶𝗻 𝗿𝗲𝘀𝗽𝗼𝗻𝘀𝗲 𝗮𝗻𝗱 𝗩𝗟𝗗𝗟 𝗽𝗿𝗼𝗱𝘂𝗰𝘁𝗶𝗼𝗻
pubmed.ncbi.nlm.nih.gov/41541501/
pubmed.ncbi.nlm.nih.gov/41541501/
New product! 📢 CHOcoCAT
A QconCAT Kit for absolute protein quantification of 29 common and critical CHO-specific host cell proteins (HCP) using LC-MS/MS.
Visit our website (lnkd.in/dyWUJrgA) for more information and save -50% 🎉 with the promo code "MyFirstCHOcoCAT" (until July 31st, 2025).
A QconCAT Kit for absolute protein quantification of 29 common and critical CHO-specific host cell proteins (HCP) using LC-MS/MS.
Visit our website (lnkd.in/dyWUJrgA) for more information and save -50% 🎉 with the promo code "MyFirstCHOcoCAT" (until July 31st, 2025).
CHOcoCAT | PolyQuant
www.polyquant.de
July 3, 2025 at 7:25 AM
New product! 📢 CHOcoCAT
A QconCAT Kit for absolute protein quantification of 29 common and critical CHO-specific host cell proteins (HCP) using LC-MS/MS.
Visit our website (lnkd.in/dyWUJrgA) for more information and save -50% 🎉 with the promo code "MyFirstCHOcoCAT" (until July 31st, 2025).
A QconCAT Kit for absolute protein quantification of 29 common and critical CHO-specific host cell proteins (HCP) using LC-MS/MS.
Visit our website (lnkd.in/dyWUJrgA) for more information and save -50% 🎉 with the promo code "MyFirstCHOcoCAT" (until July 31st, 2025).
20 years of #QconCATs! In our first newsletter of 2025 we dive into the history of QconCAT technology, highlight current #research and applications and offer a special discount on Calibration Standards and QconCAT Kits.
www.polyquant.de/_files/ugd/3...
#Proteomics #MassSpec #ProteinQuantification
www.polyquant.de/_files/ugd/3...
#Proteomics #MassSpec #ProteinQuantification
www.polyquant.de
April 1, 2025 at 7:34 AM
20 years of #QconCATs! In our first newsletter of 2025 we dive into the history of QconCAT technology, highlight current #research and applications and offer a special discount on Calibration Standards and QconCAT Kits.
www.polyquant.de/_files/ugd/3...
#Proteomics #MassSpec #ProteinQuantification
www.polyquant.de/_files/ugd/3...
#Proteomics #MassSpec #ProteinQuantification
20 years of #QconCAT research highlight from February 2018:
“Stoichiometry, Absolute Abundance, and Localization of Proteins in the Bacillus cereus Spore Coat Insoluble Fraction Determined Using a QconCAT Approach“
pubmed.ncbi.nlm.nih.gov/29260567/
#massSpectrometry #proteomics #LC-MSMS
“Stoichiometry, Absolute Abundance, and Localization of Proteins in the Bacillus cereus Spore Coat Insoluble Fraction Determined Using a QconCAT Approach“
pubmed.ncbi.nlm.nih.gov/29260567/
#massSpectrometry #proteomics #LC-MSMS
February 12, 2025 at 10:55 AM
20 years of #QconCAT research highlight from February 2018:
“Stoichiometry, Absolute Abundance, and Localization of Proteins in the Bacillus cereus Spore Coat Insoluble Fraction Determined Using a QconCAT Approach“
pubmed.ncbi.nlm.nih.gov/29260567/
#massSpectrometry #proteomics #LC-MSMS
“Stoichiometry, Absolute Abundance, and Localization of Proteins in the Bacillus cereus Spore Coat Insoluble Fraction Determined Using a QconCAT Approach“
pubmed.ncbi.nlm.nih.gov/29260567/
#massSpectrometry #proteomics #LC-MSMS
Publication alert 🚨: pubmed.ncbi.nlm.nih.gov/39884812/
#QconCAT-based targeted #proteomics to quantify the abundance of 6 transporters and 1 plasma protein marker in 5 placenta samples and associated cell lines and use of the data in a #PBPK model for IVIVE-based prediction of fetal drug exposure.
#QconCAT-based targeted #proteomics to quantify the abundance of 6 transporters and 1 plasma protein marker in 5 placenta samples and associated cell lines and use of the data in a #PBPK model for IVIVE-based prediction of fetal drug exposure.
February 3, 2025 at 11:42 AM
Publication alert 🚨: pubmed.ncbi.nlm.nih.gov/39884812/
#QconCAT-based targeted #proteomics to quantify the abundance of 6 transporters and 1 plasma protein marker in 5 placenta samples and associated cell lines and use of the data in a #PBPK model for IVIVE-based prediction of fetal drug exposure.
#QconCAT-based targeted #proteomics to quantify the abundance of 6 transporters and 1 plasma protein marker in 5 placenta samples and associated cell lines and use of the data in a #PBPK model for IVIVE-based prediction of fetal drug exposure.
We proudly present a brand-new publication, applying our #QconCAT technology in #pharmacokinetic research:
“Transporter Expressions as Part of Required Scaling Factor to Support In vitro In vivo Extrapolation for Blood-Brain Barrier Drug Permeability” pubmed.ncbi.nlm.nih.gov/39826620/ #proteomics
“Transporter Expressions as Part of Required Scaling Factor to Support In vitro In vivo Extrapolation for Blood-Brain Barrier Drug Permeability” pubmed.ncbi.nlm.nih.gov/39826620/ #proteomics
January 28, 2025 at 9:22 AM
We proudly present a brand-new publication, applying our #QconCAT technology in #pharmacokinetic research:
“Transporter Expressions as Part of Required Scaling Factor to Support In vitro In vivo Extrapolation for Blood-Brain Barrier Drug Permeability” pubmed.ncbi.nlm.nih.gov/39826620/ #proteomics
“Transporter Expressions as Part of Required Scaling Factor to Support In vitro In vivo Extrapolation for Blood-Brain Barrier Drug Permeability” pubmed.ncbi.nlm.nih.gov/39826620/ #proteomics
Congratulations on 20 years of #QconCAT technology! We are always happy to read #proteomics research articles employing our QconCATs. Throughout 2025 we will present our favourite articles, starting with the original publication.
Stay tuned for more research highlights, applications and promotions.
Stay tuned for more research highlights, applications and promotions.
Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides - PubMed
Absolute quantification in proteomics usually involves simultaneous determination of representative proteolytic peptides and stable isotope-labeled analogs. The principal limitation to widespread impl...
pubmed.ncbi.nlm.nih.gov
January 16, 2025 at 11:49 AM
Congratulations on 20 years of #QconCAT technology! We are always happy to read #proteomics research articles employing our QconCATs. Throughout 2025 we will present our favourite articles, starting with the original publication.
Stay tuned for more research highlights, applications and promotions.
Stay tuned for more research highlights, applications and promotions.
Do you want to know why we love metabolic 15N labeling for absolute protein quantification? Have a look at our latest poster, presented at this year's HUPO in Dresden. lmy.de/cpwtE
#QconCAT #SIL #SILAC #stableisotopelabeling #massspectrometry #referencestandard
#QconCAT #SIL #SILAC #stableisotopelabeling #massspectrometry #referencestandard
www.polyquant.de
December 4, 2024 at 10:37 AM
Do you want to know why we love metabolic 15N labeling for absolute protein quantification? Have a look at our latest poster, presented at this year's HUPO in Dresden. lmy.de/cpwtE
#QconCAT #SIL #SILAC #stableisotopelabeling #massspectrometry #referencestandard
#QconCAT #SIL #SILAC #stableisotopelabeling #massspectrometry #referencestandard
With Christmas approaching, we are offering our QconCATs, Calibration Standards and QconCAT Kits at special prices. Contact us to benefit from our time-limited offer and receive high quality products at lowest prices. #QconCAT #proteomics #LCMSMS #peptides #referencestandards
November 26, 2024 at 12:02 PM
With Christmas approaching, we are offering our QconCATs, Calibration Standards and QconCAT Kits at special prices. Contact us to benefit from our time-limited offer and receive high quality products at lowest prices. #QconCAT #proteomics #LCMSMS #peptides #referencestandards
Absolute Quantification of Lysosomal Proteins by Multiple Reaction Monitoring Mass Spectrometry and QconCAT Protein Standards https://www.biorxiv.org/content/10.1101/2025.01.09.632238v1
January 10, 2025 at 9:25 AM
Absolute Quantification of Lysosomal Proteins by Multiple Reaction Monitoring Mass Spectrometry and QconCAT Protein Standards https://www.biorxiv.org/content/10.1101/2025.01.09.632238v1
Absolute Quantification of Lysosomal Proteins by Multiple Reaction Monitoring Mass Spectrometry and QconCAT Protein Standards https://www.biorxiv.org/content/10.1101/2025.01.09.632238v1
January 10, 2025 at 9:25 AM
Absolute Quantification of Lysosomal Proteins by Multiple Reaction Monitoring Mass Spectrometry and QconCAT Protein Standards https://www.biorxiv.org/content/10.1101/2025.01.09.632238v1