New moment of “WTF”:
-pt with rare infection (so rare will leave unnamed, as could reveal identity)
-nl times: reach out to CDC for testing
-now: “they confirmed they are not currently accepting specimens for serology…they suggested we could reach out to Canada National Reference Center”
😳😳😳
-pt with rare infection (so rare will leave unnamed, as could reveal identity)
-nl times: reach out to CDC for testing
-now: “they confirmed they are not currently accepting specimens for serology…they suggested we could reach out to Canada National Reference Center”
😳😳😳
June 9, 2025 at 11:29 PM
New moment of “WTF”:
-pt with rare infection (so rare will leave unnamed, as could reveal identity)
-nl times: reach out to CDC for testing
-now: “they confirmed they are not currently accepting specimens for serology…they suggested we could reach out to Canada National Reference Center”
😳😳😳
-pt with rare infection (so rare will leave unnamed, as could reveal identity)
-nl times: reach out to CDC for testing
-now: “they confirmed they are not currently accepting specimens for serology…they suggested we could reach out to Canada National Reference Center”
😳😳😳
Species agnostic serology for H5: MESO SCALE DISCOVERY® (MSD) platform - a sandwich immunoassay for NP + a bridging serology assay for influenza A/H5 specific antibodies. Sucess in positive milk, and negative for naive cattle herds.
👉 www.researchsquare.com/article/rs-1...
👉 www.researchsquare.com/article/rs-1...
September 26, 2026 at 3:31 AM
Species agnostic serology for H5: MESO SCALE DISCOVERY® (MSD) platform - a sandwich immunoassay for NP + a bridging serology assay for influenza A/H5 specific antibodies. Sucess in positive milk, and negative for naive cattle herds.
👉 www.researchsquare.com/article/rs-1...
👉 www.researchsquare.com/article/rs-1...
1. We have already established that serology does not automatically imply protection or lack thereof for HBV vaccines
2. The key point with HBV protection is memory cells, which serology does not address. It is not known how forgoing recommended doses of HBV vaccine would affect the durability of...
2. The key point with HBV protection is memory cells, which serology does not address. It is not known how forgoing recommended doses of HBV vaccine would affect the durability of...
December 4, 2025 at 5:26 PM
1. We have already established that serology does not automatically imply protection or lack thereof for HBV vaccines
2. The key point with HBV protection is memory cells, which serology does not address. It is not known how forgoing recommended doses of HBV vaccine would affect the durability of...
2. The key point with HBV protection is memory cells, which serology does not address. It is not known how forgoing recommended doses of HBV vaccine would affect the durability of...
What to watch…
Waste water.
Sero-sampling (like blood bank samples or serology of close contacts).
Waste water.
Sero-sampling (like blood bank samples or serology of close contacts).
November 23, 2024 at 9:16 AM
What to watch…
Waste water.
Sero-sampling (like blood bank samples or serology of close contacts).
Waste water.
Sero-sampling (like blood bank samples or serology of close contacts).
To their credit, they did include serology to try and sort this out, but we know that serology is not very accurate, especially as there may be persistent positivity from past infection or no seroconversion after acute infection.
January 28, 2026 at 1:54 PM
To their credit, they did include serology to try and sort this out, but we know that serology is not very accurate, especially as there may be persistent positivity from past infection or no seroconversion after acute infection.
Vamos contar direito essa história: em agosto de 2024, profissionais da saúde no Missouri receberam um paciente que testou positivo para Influenza A H5, e ele não tinha histórico de contato com animais ou outras pessoas que sabidamente estavam com o vírus. Posteriormente, se confirmou o H5N1
CDC Report on Missouri H5N1 Serology Testing
CDC provides update on H5 serology testing from MO patient and contacts
www.cdc.gov
October 25, 2024 at 8:56 PM
Vamos contar direito essa história: em agosto de 2024, profissionais da saúde no Missouri receberam um paciente que testou positivo para Influenza A H5, e ele não tinha histórico de contato com animais ou outras pessoas que sabidamente estavam com o vírus. Posteriormente, se confirmou o H5N1
You probably shouldn’t rely on symptoms alone to look for novel virus spread.
We have no idea what the asymptomatic spreader potential is… although… until it adapts more… it may be low.
I would do PCR tests a few days apart, and serology at day 10-14.
We have no idea what the asymptomatic spreader potential is… although… until it adapts more… it may be low.
I would do PCR tests a few days apart, and serology at day 10-14.
This was the H5N5 patient.
"Public health officials are continuing to monitor anyone who was in contact with the patient — including more than 100 health care workers — for symptoms to ensure that human-to-human spread has not occurred."
www.washingtonpost.com/health/2025/...
"Public health officials are continuing to monitor anyone who was in contact with the patient — including more than 100 health care workers — for symptoms to ensure that human-to-human spread has not occurred."
www.washingtonpost.com/health/2025/...
Bird flu patient dies, marking second U.S. fatality in 2025
The Washington state resident, an older adult with underlying conditions, was infected with a strain that has previously been reported in animals but never before in humans.
www.washingtonpost.com
November 23, 2025 at 2:52 AM
You probably shouldn’t rely on symptoms alone to look for novel virus spread.
We have no idea what the asymptomatic spreader potential is… although… until it adapts more… it may be low.
I would do PCR tests a few days apart, and serology at day 10-14.
We have no idea what the asymptomatic spreader potential is… although… until it adapts more… it may be low.
I would do PCR tests a few days apart, and serology at day 10-14.
In a sample of around 140 LC patients and pre-pandemic controls, 83% of Long Covid patients revealed tissue-specific autoantibody signatures and immune alterations, which are not detected by routine serology in long COVID
#LongCovid
link.springer.com/article/10.1...
#LongCovid
link.springer.com/article/10.1...
Tissue-specific autoantibody signatures reveal immune alterations undetected by routine serology in long COVID - GeroScience
Long COVID affects a substantial proportion of individuals recovering from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, yet its underlying pathophysiology remains poorly und...
link.springer.com
May 13, 2026 at 11:12 PM
In a sample of around 140 LC patients and pre-pandemic controls, 83% of Long Covid patients revealed tissue-specific autoantibody signatures and immune alterations, which are not detected by routine serology in long COVID
#LongCovid
link.springer.com/article/10.1...
#LongCovid
link.springer.com/article/10.1...
The ACIP Committee which has exactly 0 general pediatricians just voted 6-4 to recommend that we do lab draws on babies to determine if they need subsequent doses of Hepatitis B vaccine. There are multiple problems with this:
December 5, 2025 at 4:03 PM
The ACIP Committee which has exactly 0 general pediatricians just voted 6-4 to recommend that we do lab draws on babies to determine if they need subsequent doses of Hepatitis B vaccine. There are multiple problems with this:
.
Amazed that people cannot learn.
Immunizations prevent sickness and death.
One college course in Immunology & Serology and you would know why.
Amazed that people cannot learn.
Immunizations prevent sickness and death.
One college course in Immunology & Serology and you would know why.
January 2, 2026 at 9:50 PM
.
Amazed that people cannot learn.
Immunizations prevent sickness and death.
One college course in Immunology & Serology and you would know why.
Amazed that people cannot learn.
Immunizations prevent sickness and death.
One college course in Immunology & Serology and you would know why.
Yup. Already had them decline to help with serology testing for a patient with an unusual infection. We were ultimately not able to confirm a suspected diagnosis, and had to wing it. Not good.
I hate to be the bearer of bad news, but my fellow Americans, we essentially no longer have a CDC.
It exists for emergency responses, but mostly in name only now.
We are on our own.
(This is information a very close, longstanding-like, 23 years long-friend passed onto me).
It exists for emergency responses, but mostly in name only now.
We are on our own.
(This is information a very close, longstanding-like, 23 years long-friend passed onto me).
August 25, 2025 at 2:37 PM
Yup. Already had them decline to help with serology testing for a patient with an unusual infection. We were ultimately not able to confirm a suspected diagnosis, and had to wing it. Not good.
Are you interested in using serology to detect hidden infections, model immune responses, and estimate epidemic dynamics? You may be interested in the new 1.2.0 release of the R package, serosolver: seroanalytics.org/serosolver/i...
September 18, 2026 at 7:47 PM
Are you interested in using serology to detect hidden infections, model immune responses, and estimate epidemic dynamics? You may be interested in the new 1.2.0 release of the R package, serosolver: seroanalytics.org/serosolver/i...
People started looking into different things the govt wasn’t working on. H5N1 in retail milk, basic virology/pathogenesis, serology, phylogenetics, preclinical vaccine studies, antiviral drug resistance, etc. It improved our understanding of the current H5N1 situation. Guys, it’s really fucking bad.
February 9, 2025 at 3:37 PM
People started looking into different things the govt wasn’t working on. H5N1 in retail milk, basic virology/pathogenesis, serology, phylogenetics, preclinical vaccine studies, antiviral drug resistance, etc. It improved our understanding of the current H5N1 situation. Guys, it’s really fucking bad.
Serology tests for everyone! Make sure you are still inoculated. I worked in a hospital and had serology tests and required an updated MMR vaccine.
Wow!
So 1+1 really does equal 2.
The Texas measles outbreak is hitting the area with the highest vaccine exemption rates. Shocking—if you ignore science.
So 1+1 really does equal 2.
The Texas measles outbreak is hitting the area with the highest vaccine exemption rates. Shocking—if you ignore science.
February 24, 2025 at 8:56 PM
Serology tests for everyone! Make sure you are still inoculated. I worked in a hospital and had serology tests and required an updated MMR vaccine.
Except the piece doesn't compare apples with apples, no idea who was tested or if any with both methods, doesn't describe the methods used for PCR or serology, claims PCR was wrong when in fact serology may have been insensitive, conducted poorly or used samples that were collected too early.
Only 14% of Positive PCR Tests Meet Study’s Definition of Infection: Journal 'Frontiers in Epidemiology'
open.substack.com/pub/jonfleet...
open.substack.com/pub/jonfleet...
Only 14% of Positive PCR Tests Meet Study’s Definition of Infection: Journal 'Frontiers in Epidemiology'
"A PCR-positive test alone can by no means confirm infection," study authors confirm—yet the test is currently being used to justify government response to bird flu.
open.substack.com
December 27, 2025 at 12:00 PM
Except the piece doesn't compare apples with apples, no idea who was tested or if any with both methods, doesn't describe the methods used for PCR or serology, claims PCR was wrong when in fact serology may have been insensitive, conducted poorly or used samples that were collected too early.
Also to recommend widespread serology instead of PCR or RAT is also really dumb
January 26, 2025 at 12:57 AM
Also to recommend widespread serology instead of PCR or RAT is also really dumb
Yesterday was one of the darkest days in NIH history, all because Jay Bhattacharya was so outraged that he was criticized for a shitty serology study that he went all in on burning it all down.
www.scientificamerican.com/article/nih-...
www.scientificamerican.com/article/nih-...
NIH Director Removes Four Main Scientists amid Massive Staff Purge
The Trump Administration has fired four leaders and thousands of employees at the National Institutes of Health in "one of the darkest days"
www.scientificamerican.com
April 2, 2025 at 11:00 AM
Yesterday was one of the darkest days in NIH history, all because Jay Bhattacharya was so outraged that he was criticized for a shitty serology study that he went all in on burning it all down.
www.scientificamerican.com/article/nih-...
www.scientificamerican.com/article/nih-...
I deleted my previous post. It was poorly worded.
The Nebraska lab has started testing the 16 passengers w/ their own PCR.
CDC guidance is to test once symptomatic because they only have a serology (antibody) test.
My guess is Nebraska is doing PCR to confirm these people don’t have the virus.
The Nebraska lab has started testing the 16 passengers w/ their own PCR.
CDC guidance is to test once symptomatic because they only have a serology (antibody) test.
My guess is Nebraska is doing PCR to confirm these people don’t have the virus.
Remember PCR tests from Covid? The CDC doesn't have one for the Andes virus.
I learned that a lab in Nebraska built and validated an Andes-specific PCR test in literal days in anticipation of receiving the American cruise ship passengers.
My story for @wired.com: www.wired.com/story/race-t...
I learned that a lab in Nebraska built and validated an Andes-specific PCR test in literal days in anticipation of receiving the American cruise ship passengers.
My story for @wired.com: www.wired.com/story/race-t...
Inside the Race to Develop a Test for the Rare Andes Hantavirus
A University of Nebraska lab has developed a test that can detect the virus before symptoms become severe. Now, it's ready to start testing those returning to the US after a cruise outbreak.
www.wired.com
May 14, 2026 at 10:19 PM
I deleted my previous post. It was poorly worded.
The Nebraska lab has started testing the 16 passengers w/ their own PCR.
CDC guidance is to test once symptomatic because they only have a serology (antibody) test.
My guess is Nebraska is doing PCR to confirm these people don’t have the virus.
The Nebraska lab has started testing the 16 passengers w/ their own PCR.
CDC guidance is to test once symptomatic because they only have a serology (antibody) test.
My guess is Nebraska is doing PCR to confirm these people don’t have the virus.
HUGE outbreak of HPAI in Norwegian seabirds, w "environmental contamination" of sheep grazing areas. 1/220 sheep tested positive by serology.
👉 onlinelibrary.wiley.com/doi/10.1111/...
👉 onlinelibrary.wiley.com/doi/10.1111/...
November 27, 2025 at 6:25 AM
HUGE outbreak of HPAI in Norwegian seabirds, w "environmental contamination" of sheep grazing areas. 1/220 sheep tested positive by serology.
👉 onlinelibrary.wiley.com/doi/10.1111/...
👉 onlinelibrary.wiley.com/doi/10.1111/...
Continued spread of #birdflu is concerning.
Critical to understand
1. How the child was exposed
2. Although low levels of virus detection, important to ensure other contacts are followed up and tested (serology and PCR)
3. What the child's symptoms were
Critical to understand
1. How the child was exposed
2. Although low levels of virus detection, important to ensure other contacts are followed up and tested (serology and PCR)
3. What the child's symptoms were
New from California's health department, now probing with CDC first U.S. case of bird flu in a child
- "not likely infectious"
- "also positive for respiratory viruses"
- "a possible exposure to wild birds"
- "attended daycare with mild symptoms"
www.cdph.ca.gov/Programs/OPA...
- "not likely infectious"
- "also positive for respiratory viruses"
- "a possible exposure to wild birds"
- "attended daycare with mild symptoms"
www.cdph.ca.gov/Programs/OPA...
November 19, 2024 at 6:59 PM
Continued spread of #birdflu is concerning.
Critical to understand
1. How the child was exposed
2. Although low levels of virus detection, important to ensure other contacts are followed up and tested (serology and PCR)
3. What the child's symptoms were
Critical to understand
1. How the child was exposed
2. Although low levels of virus detection, important to ensure other contacts are followed up and tested (serology and PCR)
3. What the child's symptoms were
Some really good news from Inside Medicine: the flight attendant who developed symptoms has tested negative for hantavirus on both PCR and serology. Some relevant points here 🧵
Scoop: Dutch flight attendant tests negative for Andes virus.
The WHO confirmed the information to Inside Medicine, saying that the flight attendant's symptoms were due to some other cause.
open.substack.com
May 7, 2026 at 9:32 PM
Some really good news from Inside Medicine: the flight attendant who developed symptoms has tested negative for hantavirus on both PCR and serology. Some relevant points here 🧵
Says here that the replaced studies were about cases involving infected cats, wastewater, & veterinarian serology. What would these studies show?
(I read the cat paper, the others are well-informed guesses)
1. Cats get & die from H5N1
2. Shit ton of H5N1 in wastewater
3. Infected & undetected vets
(I read the cat paper, the others are well-informed guesses)
1. Cats get & die from H5N1
2. Shit ton of H5N1 in wastewater
3. Infected & undetected vets
February 9, 2025 at 3:37 PM
Says here that the replaced studies were about cases involving infected cats, wastewater, & veterinarian serology. What would these studies show?
(I read the cat paper, the others are well-informed guesses)
1. Cats get & die from H5N1
2. Shit ton of H5N1 in wastewater
3. Infected & undetected vets
(I read the cat paper, the others are well-informed guesses)
1. Cats get & die from H5N1
2. Shit ton of H5N1 in wastewater
3. Infected & undetected vets
CDC confirms D1.3 genotype in recent H5N1 case in Ohio
In other updates, the CDC said serology testing ruled out human-to-human spread in a California child's illness earlier this year.
www.cidrap.umn.edu/a...
In other updates, the CDC said serology testing ruled out human-to-human spread in a California child's illness earlier this year.
www.cidrap.umn.edu/a...
March 20, 2025 at 8:55 PM
CDC confirms D1.3 genotype in recent H5N1 case in Ohio
In other updates, the CDC said serology testing ruled out human-to-human spread in a California child's illness earlier this year.
www.cidrap.umn.edu/a...
In other updates, the CDC said serology testing ruled out human-to-human spread in a California child's illness earlier this year.
www.cidrap.umn.edu/a...
Elegant studies using high-throughput serology showed substantial loss (on the order of 10–70%) of pre-existing antibody repertoires after measles infection. Epidemiologically, this maps onto increased non-measles infectious mortality for 2–3 years after outbreaks.
April 11, 2026 at 11:24 AM
Elegant studies using high-throughput serology showed substantial loss (on the order of 10–70%) of pre-existing antibody repertoires after measles infection. Epidemiologically, this maps onto increased non-measles infectious mortality for 2–3 years after outbreaks.
.
All RFK has to do is study one college course,
Immunology & Serology
and he would know.
All RFK has to do is study one college course,
Immunology & Serology
and he would know.
December 1, 2025 at 12:33 AM
.
All RFK has to do is study one college course,
Immunology & Serology
and he would know.
All RFK has to do is study one college course,
Immunology & Serology
and he would know.