Excited to see this new #glycotime work from #proudofalumni Simon Wisnovsky's lab at UBC, identification of ST3GAL4 as mediator of Siglec-9 ligand biosynthesis in AML cells, which promotes immune evasion especially of macrophages.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
The glycosyltransferase ST3GAL4 drives immune evasion in acute myeloid leukemia by synthesizing ligands for the glyco-immune checkpoint receptor Siglec-9 - Leukemia
Leukemia - The glycosyltransferase ST3GAL4 drives immune evasion in acute myeloid leukemia by synthesizing ligands for the glyco-immune checkpoint receptor Siglec-9
www.nature.com
November 19, 2024 at 7:37 AM
Excited to see this new #glycotime work from #proudofalumni Simon Wisnovsky's lab at UBC, identification of ST3GAL4 as mediator of Siglec-9 ligand biosynthesis in AML cells, which promotes immune evasion especially of macrophages.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Excited to share our laboratory's research tools to study Siglecs along with some interesting discoveries with these tools. Lot of help from many group, but @zeinab-jc.bsky.social was the major driver of this work. Please reach out if you would like to try them out. www.science.org/doi/10.1126/...
An ultrasensitive and modular platform to detect Siglec ligands and control immune cell function
Siglec-liposomes are a multivalent and modular platform for profiling Siglec ligands and modulating immune cell function.
www.science.org
November 12, 2025 at 9:57 PM
Excited to share our laboratory's research tools to study Siglecs along with some interesting discoveries with these tools. Lot of help from many group, but @zeinab-jc.bsky.social was the major driver of this work. Please reach out if you would like to try them out. www.science.org/doi/10.1126/...
Our latest work on Siglec binding to sulfated glycans. Great help: @khookh.bsky.social, @pengwu.bsky.social ,@raflynn5.bsky.social. Fom 1st PhD graduate of the lab (Jaesoo Jung) and help from Edward, Zeinab, Jhon, Kelli, @tayloregray.bsky.social, and Chris from my lab.
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Understanding the Glycosylation Pathways Involved in the Biosynthesis of the Sulfated Glycan Ligands for Siglecs
Carbohydrate sulfation plays a pivotal role in modulating the strength of Siglec–glycan interactions. Recently, new aspects of Siglec binding to sulfated cell surface carbohydrates have been discovere...
pubs.acs.org
January 22, 2025 at 2:32 AM
Our latest work on Siglec binding to sulfated glycans. Great help: @khookh.bsky.social, @pengwu.bsky.social ,@raflynn5.bsky.social. Fom 1st PhD graduate of the lab (Jaesoo Jung) and help from Edward, Zeinab, Jhon, Kelli, @tayloregray.bsky.social, and Chris from my lab.
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
New work from @siglecdude.bsky.social sheds light on cis and trans sulfate ligands for Siglec family members #glycotime
Our latest work on Siglec binding to sulfated glycans. Great help: @khookh.bsky.social, @pengwu.bsky.social ,@raflynn5.bsky.social. Fom 1st PhD graduate of the lab (Jaesoo Jung) and help from Edward, Zeinab, Jhon, Kelli, @tayloregray.bsky.social, and Chris from my lab.
pubs.acs.org/doi/10.1021/...
pubs.acs.org/doi/10.1021/...
Understanding the Glycosylation Pathways Involved in the Biosynthesis of the Sulfated Glycan Ligands for Siglecs
Carbohydrate sulfation plays a pivotal role in modulating the strength of Siglec–glycan interactions. Recently, new aspects of Siglec binding to sulfated cell surface carbohydrates have been discovere...
pubs.acs.org
January 22, 2025 at 5:54 AM
New work from @siglecdude.bsky.social sheds light on cis and trans sulfate ligands for Siglec family members #glycotime
Good candidate modality for bolting on a sialidase (pubmed.ncbi.nlm.nih.gov/31143782/)
November 19, 2024 at 5:43 PM
Good candidate modality for bolting on a sialidase (pubmed.ncbi.nlm.nih.gov/31143782/)
Happy to share this #glycotime collaborative work with Jim Brooks lab @stanfordmedicine.bsky.social just out @jclinical-invest.bsky.social. Siglec/sialoglycan axis mediates immune suppression in prostate cancer.
www.jci.org/articles/vie...
www.jci.org/articles/vie...
www.jci.org
December 17, 2024 at 4:31 PM
Happy to share this #glycotime collaborative work with Jim Brooks lab @stanfordmedicine.bsky.social just out @jclinical-invest.bsky.social. Siglec/sialoglycan axis mediates immune suppression in prostate cancer.
www.jci.org/articles/vie...
www.jci.org/articles/vie...
Corrected link: www.jci.org/articles/vie...
JCI -
Sialylated glycoproteins suppress immune cell killing by binding to Siglec-7 and Siglec-9 in prostate cancer
www.jci.org
December 17, 2024 at 4:43 PM
Corrected link: www.jci.org/articles/vie...
✅ An exciting work from Dr. Macauley’s lab (@siglecdude.bsky.social)!
📣📣 Highly recommend to read and share this awesome story! @zeinab-jc.bsky.social #glycotime, #glycoimmunology, #Siglec, #Siglec-Liposome-tools, @glyconet.bsky.social
📣📣 Highly recommend to read and share this awesome story! @zeinab-jc.bsky.social #glycotime, #glycoimmunology, #Siglec, #Siglec-Liposome-tools, @glyconet.bsky.social
Very excited to share some work the Lab has been working on for a while. A new platform to probe Siglec ligands. Led by an incredible PhD student (Zeinab). Comes out of an ongoing fruitful collaboration with the Derda lab. Very open to feedback! www.biorxiv.org/content/10.1...
An ultrasensitive and modular platform to detect Siglec ligands and control immune cell function
Siglecs are immunomodulatory receptors that regulate immune cell function. A fundamental challenge in studying Siglec-ligand interactions is the low affinity of Siglecs for their ligands. Inspired by ...
www.biorxiv.org
June 15, 2025 at 6:51 PM
✅ An exciting work from Dr. Macauley’s lab (@siglecdude.bsky.social)!
📣📣 Highly recommend to read and share this awesome story! @zeinab-jc.bsky.social #glycotime, #glycoimmunology, #Siglec, #Siglec-Liposome-tools, @glyconet.bsky.social
📣📣 Highly recommend to read and share this awesome story! @zeinab-jc.bsky.social #glycotime, #glycoimmunology, #Siglec, #Siglec-Liposome-tools, @glyconet.bsky.social
Quando essas moléculas de açúcar se ligam aos receptores Siglec na superfície das células imunes, elas emitem sinais inibitórios, fazendo com que o sistema imunológico "abaixe as armas" e não ataque o tumor. Essa é uma das razões pelas quais muitos tumores ainda conseguem crescer … 6/👇
January 4, 2026 at 1:58 PM
Quando essas moléculas de açúcar se ligam aos receptores Siglec na superfície das células imunes, elas emitem sinais inibitórios, fazendo com que o sistema imunológico "abaixe as armas" e não ataque o tumor. Essa é uma das razões pelas quais muitos tumores ainda conseguem crescer … 6/👇
Nociceptor neurons suppress alveolar macrophage-induced Siglec-F+ neutrophil-mediated inflammation to protect against pulmonary fibrosis: @cp-immunity.bsky.social www.cell.com/immunity/ful...
May 30, 2025 at 3:38 PM
Nociceptor neurons suppress alveolar macrophage-induced Siglec-F+ neutrophil-mediated inflammation to protect against pulmonary fibrosis: @cp-immunity.bsky.social www.cell.com/immunity/ful...
Very cool study showing how membrane lipids actively contribute to Siglec-6 binding — indicating that membrane lipid composition can modulate ligand recognition! #lipidtime
When glycan recognition is linked to immune response, you need fine tuning! 😎
Here ⬇️ we show how human Siglecs act as molecular precision tools, recognising the glycan and also the biological environment (or context) these glycans are found in. A 1/5 #glycotime🧵 ⬇️
doi.org/10.1101/2025...
Here ⬇️ we show how human Siglecs act as molecular precision tools, recognising the glycan and also the biological environment (or context) these glycans are found in. A 1/5 #glycotime🧵 ⬇️
doi.org/10.1101/2025...
June 22, 2025 at 3:20 PM
Very cool study showing how membrane lipids actively contribute to Siglec-6 binding — indicating that membrane lipid composition can modulate ligand recognition! #lipidtime
New work on the structure of Siglec-10. Great collaboration that was solidified with a visit to Spain last year with fantastic discussions with June Ereno-Orbea and Jesus Jimenez-Barbero. Nice contributions on modeling by @elisafadda.bsky.social.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
The unique molecular recognition features of Siglec-10: structural insights into sialoglycan and antibody interactions
Siglec-10 is a sialic acid-binding immunoglobulin-like lectin implicated in immune regulation, yet its molecular basis for ligand recognition and functional role in immune modulation remain poorly def...
www.biorxiv.org
June 12, 2025 at 1:26 PM
New work on the structure of Siglec-10. Great collaboration that was solidified with a visit to Spain last year with fantastic discussions with June Ereno-Orbea and Jesus Jimenez-Barbero. Nice contributions on modeling by @elisafadda.bsky.social.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
👇 #Siglec-7 NMR assignment and binding studies with #gangliosides, impact of ligand flexibility #nmr #3D #glycan #glycotime #GD3 #structuralbiology #biophysics @robertamarchetti.bsky.social @molinarotony.bsky.social
advanced.onlinelibrary.wiley.com/doi/10.1002/...
advanced.onlinelibrary.wiley.com/doi/10.1002/...
Insights into Siglec‐7 Binding to Gangliosides: NMR Protein Assignment and the Impact of Ligand Flexibility
The molecular recognition of the sugar moiety of GD3 and Gb3 (globotriaosylceramide) derivatives by Siglec-7 has been thoroughly studied offering insights for cancer diagnostics and therapies. A comb....
advanced.onlinelibrary.wiley.com
April 26, 2025 at 1:22 PM
👇 #Siglec-7 NMR assignment and binding studies with #gangliosides, impact of ligand flexibility #nmr #3D #glycan #glycotime #GD3 #structuralbiology #biophysics @robertamarchetti.bsky.social @molinarotony.bsky.social
advanced.onlinelibrary.wiley.com/doi/10.1002/...
advanced.onlinelibrary.wiley.com/doi/10.1002/...
👇👇 Insights into stability, dimerisation, and ligand binding properties of Siglec-7: Isotope labelling in HEK293 cells for protein characterisation by NMR #Siglec #NMR #glycotime, @robertamarchetti.bsky.social
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Insights into stability, dimerisation, and ligand binding properties of Siglec-7: Isotope labelling in HEK293 cells for protein characterisation by NMR spectroscopy
Siglec-7, an immune checkpoint receptor, has emerged as a promising target for cancer immunotherapy due to its involvement in the regulation of immune…
www.sciencedirect.com
April 10, 2025 at 11:32 AM
👇👇 Insights into stability, dimerisation, and ligand binding properties of Siglec-7: Isotope labelling in HEK293 cells for protein characterisation by NMR #Siglec #NMR #glycotime, @robertamarchetti.bsky.social
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
A fab, sunshiny week in 🇩🇰! Great #glycotime 🧪 at the CBM16 in Helsingør, with posters
Bea: regulation of N-glycosylation by OST (doi.org/10.1101/2025...)
Silvia: Siglec-6 high-recision recognition of glycolipids (doi.org/10.1038/s420...)
Ojas: de novo binders design (doi.org/10.64898/202...)
🧵⬇️
Bea: regulation of N-glycosylation by OST (doi.org/10.1101/2025...)
Silvia: Siglec-6 high-recision recognition of glycolipids (doi.org/10.1038/s420...)
Ojas: de novo binders design (doi.org/10.64898/202...)
🧵⬇️
May 7, 2026 at 4:43 PM
A fab, sunshiny week in 🇩🇰! Great #glycotime 🧪 at the CBM16 in Helsingør, with posters
Bea: regulation of N-glycosylation by OST (doi.org/10.1101/2025...)
Silvia: Siglec-6 high-recision recognition of glycolipids (doi.org/10.1038/s420...)
Ojas: de novo binders design (doi.org/10.64898/202...)
🧵⬇️
Bea: regulation of N-glycosylation by OST (doi.org/10.1101/2025...)
Silvia: Siglec-6 high-recision recognition of glycolipids (doi.org/10.1038/s420...)
Ojas: de novo binders design (doi.org/10.64898/202...)
🧵⬇️
Siglec = Sialic acid-binding immunoglobulin-type lectin
When glycan recognition is linked to immune response, you need fine tuning! 😎
Here ⬇️ we show how human Siglecs act as molecular precision tools, recognising the glycan and also the biological environment (or context) these glycans are found in. A 1/5 #glycotime🧵 ⬇️
doi.org/10.1101/2025...
Here ⬇️ we show how human Siglecs act as molecular precision tools, recognising the glycan and also the biological environment (or context) these glycans are found in. A 1/5 #glycotime🧵 ⬇️
doi.org/10.1101/2025...
June 22, 2025 at 12:35 PM
Siglec = Sialic acid-binding immunoglobulin-type lectin
Our review on the recent acceleration of research into carbohydrate Sulfation as as an enhancer of Siglec ligands: www.sciencedirect.com/science/arti...
Redirecting
kwnsfk27.r.eu-west-1.awstrack.me
May 5, 2025 at 8:54 PM
Our review on the recent acceleration of research into carbohydrate Sulfation as as an enhancer of Siglec ligands: www.sciencedirect.com/science/arti...
Very excited to share some work the Lab has been working on for a while. A new platform to probe Siglec ligands. Led by an incredible PhD student (Zeinab). Comes out of an ongoing fruitful collaboration with the Derda lab. Very open to feedback! www.biorxiv.org/content/10.1...
An ultrasensitive and modular platform to detect Siglec ligands and control immune cell function
Siglecs are immunomodulatory receptors that regulate immune cell function. A fundamental challenge in studying Siglec-ligand interactions is the low affinity of Siglecs for their ligands. Inspired by ...
www.biorxiv.org
June 14, 2025 at 9:05 PM
Very excited to share some work the Lab has been working on for a while. A new platform to probe Siglec ligands. Led by an incredible PhD student (Zeinab). Comes out of an ongoing fruitful collaboration with the Derda lab. Very open to feedback! www.biorxiv.org/content/10.1...
Lectins are known to be low affinity binders with relatively broad glycan target preference. In this work live in @commsbio.nature.com we show how Siglecs change this paradigm, acting as molecular precision tools required to fine tune immune response 🤯 #glycotime 🧵⬇️
doi.org/10.1038/s420...
doi.org/10.1038/s420...
Glycolipid recognition and binding by Siglec-6 hinges on interactions with the cell membrane - Communications Biology
Siglecs are immunoregulatory lectins with very similar architectures. The authors show how the V-set domain of Siglec-6 allows it to select for precise sialylation patterns in specific biological envi...
doi.org
March 2, 2026 at 5:27 PM
Lectins are known to be low affinity binders with relatively broad glycan target preference. In this work live in @commsbio.nature.com we show how Siglecs change this paradigm, acting as molecular precision tools required to fine tune immune response 🤯 #glycotime 🧵⬇️
doi.org/10.1038/s420...
doi.org/10.1038/s420...
Glaubts SARS-Cov-2 kann nur durch ACE in die Zellen? Weit gefehlt
ACE unabhängige Faktoren:
CD147, AXL, CD169/Siglec-1, CD209L, CD209, CLEC4G, ASGR1, LDLRAD3, TMEM30A, TMEM106B, Transferrinrezeptor 1, GPR78, Integrin α5β1, KREMEN1, LFA-1 & CD4
Somit könnt ihr Blocken vergessen
ACE unabhängige Faktoren:
CD147, AXL, CD169/Siglec-1, CD209L, CD209, CLEC4G, ASGR1, LDLRAD3, TMEM30A, TMEM106B, Transferrinrezeptor 1, GPR78, Integrin α5β1, KREMEN1, LFA-1 & CD4
Somit könnt ihr Blocken vergessen
December 24, 2025 at 7:57 PM
Glaubts SARS-Cov-2 kann nur durch ACE in die Zellen? Weit gefehlt
ACE unabhängige Faktoren:
CD147, AXL, CD169/Siglec-1, CD209L, CD209, CLEC4G, ASGR1, LDLRAD3, TMEM30A, TMEM106B, Transferrinrezeptor 1, GPR78, Integrin α5β1, KREMEN1, LFA-1 & CD4
Somit könnt ihr Blocken vergessen
ACE unabhängige Faktoren:
CD147, AXL, CD169/Siglec-1, CD209L, CD209, CLEC4G, ASGR1, LDLRAD3, TMEM30A, TMEM106B, Transferrinrezeptor 1, GPR78, Integrin α5β1, KREMEN1, LFA-1 & CD4
Somit könnt ihr Blocken vergessen
Sharing our recent pre-print where we drill down further into the transcriptional regulation of Siglec ligands. Here we show that tissue specific regulatory networks dictate changes in sialoglycan patterns. Work led by Rebecca Garnham and postdoc Adam Duxfield.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
The Androgen Receptor and MYC synergise to modulate the synthesis of Siglec-7 ligands in prostate cancer
Glyco-immune checkpoints have recently been shown to be critical mediators of immunotherapy resistance across multiple cancer types. In clinical trials, immunotherapeutic treatments for prostate cance...
www.biorxiv.org
October 20, 2025 at 11:50 AM
Sharing our recent pre-print where we drill down further into the transcriptional regulation of Siglec ligands. Here we show that tissue specific regulatory networks dictate changes in sialoglycan patterns. Work led by Rebecca Garnham and postdoc Adam Duxfield.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Sharing our latest work on B cell Siglecs in regulating antibody production. Unlike our previous work (PMID: 35294874), where we found that CD22 (Siglec-2) positively impacts the germinal center (GC), our new work uncovers a regulatory role for Siglec-G in the GC: www.biorxiv.org/content/10.6...
May 11, 2026 at 1:45 PM
Sharing our latest work on B cell Siglecs in regulating antibody production. Unlike our previous work (PMID: 35294874), where we found that CD22 (Siglec-2) positively impacts the germinal center (GC), our new work uncovers a regulatory role for Siglec-G in the GC: www.biorxiv.org/content/10.6...
Amazing study. “SIGLEC-3 (CD33) serves as an immune checkpoint receptor for HBV infection” www.jci.org/articles/vie...
October 5, 2023 at 1:09 AM
Amazing study. “SIGLEC-3 (CD33) serves as an immune checkpoint receptor for HBV infection” www.jci.org/articles/vie...
The receptor binding domain of SARS-CoV-2 Omicron subvariants targets Siglec-9 to decrease its immunogenicity by preventing macrophage phagocytosis
A mutation in Spike, present in SARS-CoV-2 Omicron. reduces its immunogenicity.
www.nature.com/artic...
1/3
A mutation in Spike, present in SARS-CoV-2 Omicron. reduces its immunogenicity.
www.nature.com/artic...
1/3
The receptor binding domain of SARS-CoV-2 Omicron subvariants targets Siglec-9 to decrease its immunogenicity by preventing macrophage phagocytosis
Nature Immunology - Zhang and colleagues found that Omicron RBD binding to Siglec-9 impaired phagocytosis and antigen presentation in macrophages, an effect abrogated by an F375S mutation in the...
www.nature.com
March 8, 2024 at 2:25 PM
The receptor binding domain of SARS-CoV-2 Omicron subvariants targets Siglec-9 to decrease its immunogenicity by preventing macrophage phagocytosis
A mutation in Spike, present in SARS-CoV-2 Omicron. reduces its immunogenicity.
www.nature.com/artic...
1/3
A mutation in Spike, present in SARS-CoV-2 Omicron. reduces its immunogenicity.
www.nature.com/artic...
1/3