This randomized trial assesses the effect of intratracheal budesonide mixed with surfactant on survival free of bronchopulmonary dysplasia in extremely preterm infants.

Tsai, Zhang, Scholten et al. develop a surfactant- assisted one-pot sample preparation coupled with mass spectrometry method (SOP-MS) for label-free global single-cell proteomics. This method allows r...

track by Eric Heisman and the Formation

AbstractHydrogen/Deuterium eXchange (HDX) methods for studying protein dynamics would benefit from millisecond-scale incubations to probe intrinsically disordered proteins, highly dynamic regions, and conformation changes. Here, we investigate droplet microfluidics for rapid mixing to trigger D2O labeling, uniform incubations, and rapid droplet merging for acid quenching in advance of mass spectrometry. A surfactant-free merging approach combining expansion elements for synchronized droplet collision proved robust. The high diffusive flux of D2O and protons enables microsecond mixing to trigger and arrest D2O labeling, respectively, affording the possibility of single millisecond incubations. Droplet HDX processors were used to measure the fast uptake characteristics of a model peptide. Forward exchange measurements demonstrate D2O labeling to be the rate-limiting step, in essence defining 10 milliseconds as the minimum practical incubation time for proteins at room temperature, pD 7.4. With the ability to access millisecond time scales, the fast dynamics of calmodulin, a model of calcium-triggered allostery with rapid conformational switching, was investigated. At 10 milliseconds, we could observe significant deuterium uptake within the well-defined EF-hand Ca2+ binding motifs. These findings demonstrate that millisecond HDX enabled by droplet microfluidics allows areas of heightened plasticity to be detected within a stably folded protein.

Rapid, sensitive, and efficient detection of pathogenic bacteria is crucial for public health, particularly in food safety, environmental monitoring, and clinical diagnostics. In this paper, we report a simple sol–gel-based synthesis of poly(o-phenylenediamine) (PoPD) and surfactant-free gold nanoparticles (

Surfactant-Free, Ube-Enriched Perianal Optimized Cleanser.

In the headlines: A one thousand dollar Newborn Support Grant takes effect and Government to provide free surfactant therapy for premature babies For details on these stories and more, visit www.govt...

This randomized trial assesses the effect of intratracheal budesonide mixed with surfactant on survival free of bronchopulmonary dysplasia in extremely preterm infants.

Size control over nanomaterials obtained by simple, environmentally friendly and cost-efficient methods is a key achievement to make of the most the u…