Choanoflagellate transfection just got much cheaper: www.biorxiv.org/content/10.6...
Congratulations @frutag33.bsky.social & team. #choanoflagellates #transfection
Congratulations @frutag33.bsky.social & team. #choanoflagellates #transfection
March 12, 2026 at 8:23 PM
Choanoflagellate transfection just got much cheaper: www.biorxiv.org/content/10.6...
Congratulations @frutag33.bsky.social & team. #choanoflagellates #transfection
Congratulations @frutag33.bsky.social & team. #choanoflagellates #transfection
Problems with transfection? Try an electric eel...
t.co/8I6kAQVRrZ
t.co/8I6kAQVRrZ
December 8, 2023 at 6:43 AM
Problems with transfection? Try an electric eel...
t.co/8I6kAQVRrZ
t.co/8I6kAQVRrZ
Breakthrough in MSC therapy! APOs@BP boosts transfection efficiency 10.4x with enhanced cell viability in serum. #StemCellResearch PMID:40016275, Nat Commun 2025, @NatureComms https://doi.org/10.1038/s41467-025-57278-8 #Medsky #Pharmsky #RNAsky 🧪
Serum-tolerant polymeric complex for stem-cell transfection and neural differentiation | Nature Communications
Mesenchymal stem cell (MSC) therapy holds promise in biomedical applications but faces challenges in efficient transfection without compromising cell viability. Here, we show a serum-tolerant MSC transfection nanotool, APOs@BP, composed of an apolipoprotein (APO) corona and a boronated polyethyleneimine (BP) core. The APOs corona’s serum-protein resistance and cytomembrane affinity enable APOs@BP to achieve 10.4-fold higher transfection efficiency and improved cytocompatibility in serum-containing medium compared to high-molecular-weight polycationic transfectants. For MSC neural differentiation, miRNA-124 and all-trans retinoic acid derivative (atRAN) are further loaded into APOs@BP, forming a polymeric complex for sequential drug release triggered by lysosomal acid and cytosolic reactive oxygen species post-transplantation. Transcriptomic analysis confirms that this system enhances MSC neural differentiation through sequential activation of atRAN-induced differentiation potential and
doi.org
March 15, 2025 at 12:10 AM
Breakthrough in MSC therapy! APOs@BP boosts transfection efficiency 10.4x with enhanced cell viability in serum. #StemCellResearch PMID:40016275, Nat Commun 2025, @NatureComms https://doi.org/10.1038/s41467-025-57278-8 #Medsky #Pharmsky #RNAsky 🧪
Streaking plates, growing cultures, running a miniprep. It's not hard, just tedious.
Starting with Transfection-Ready DNA lets you skip the prep.
https://twp.ai/4hvlbd
#Plasmids #LabLife
Starting with Transfection-Ready DNA lets you skip the prep.
https://twp.ai/4hvlbd
#Plasmids #LabLife
October 1, 2026 at 2:00 PM
Streaking plates, growing cultures, running a miniprep. It's not hard, just tedious.
Starting with Transfection-Ready DNA lets you skip the prep.
https://twp.ai/4hvlbd
#Plasmids #LabLife
Starting with Transfection-Ready DNA lets you skip the prep.
https://twp.ai/4hvlbd
#Plasmids #LabLife
Excited to announce the launch of Transfection-Ready DNA — giving academic and industry researchers access to 27,000+ plasmids from Addgene's trusted repository as high-purity, NGS sequence-verified DNA.
Read the press release: https://twp.ai/4hvlXn
#Plasmids #Transfection
Read the press release: https://twp.ai/4hvlXn
#Plasmids #Transfection
September 15, 2026 at 1:00 PM
Excited to announce the launch of Transfection-Ready DNA — giving academic and industry researchers access to 27,000+ plasmids from Addgene's trusted repository as high-purity, NGS sequence-verified DNA.
Read the press release: https://twp.ai/4hvlXn
#Plasmids #Transfection
Read the press release: https://twp.ai/4hvlXn
#Plasmids #Transfection
Isadonna Tengganu and Ke Hu develop transfection and ExM protocols for Chromera velia, enabling direct comparisons with its parasitic apicomplexan relative, Toxoplasma gondi.
journals.biologists.com/jcs/article/...
journals.biologists.com/jcs/article/...
January 13, 2026 at 9:53 AM
Isadonna Tengganu and Ke Hu develop transfection and ExM protocols for Chromera velia, enabling direct comparisons with its parasitic apicomplexan relative, Toxoplasma gondi.
journals.biologists.com/jcs/article/...
journals.biologists.com/jcs/article/...
Tranformation
Transfection
Infection
what’s the difference?
1/4
Transfection
Infection
what’s the difference?
1/4
September 20, 2025 at 8:37 AM
Tranformation
Transfection
Infection
what’s the difference?
1/4
Transfection
Infection
what’s the difference?
1/4
📢On June 1st, we will be launching CenSpark650, the very first selective fluorescent probe for imaging centrioles and cilia in live cells.🔬 No transfection, zero genetic manipulation. Just Add & Image 1 hour later. Learn more: spirochrome.com/12-may-2026/
#Bioimaging
#Bioimaging
May 27, 2026 at 3:23 PM
📢On June 1st, we will be launching CenSpark650, the very first selective fluorescent probe for imaging centrioles and cilia in live cells.🔬 No transfection, zero genetic manipulation. Just Add & Image 1 hour later. Learn more: spirochrome.com/12-may-2026/
#Bioimaging
#Bioimaging
Our first time transfection zsgreen when using transparent media. Did not expect this 😲
November 14, 2024 at 9:25 PM
Our first time transfection zsgreen when using transparent media. Did not expect this 😲
27,000+ plasmids, curated from Addgene's repository of 170,000+ constructs — spanning CRISPR, imaging and reporters, and gene expression — now available as ready-to-pipette Transfection-Ready DNA. And more to come!
https://twp.ai/4hvlat
#Plasmids #CRISPR
https://twp.ai/4hvlat
#Plasmids #CRISPR
September 28, 2026 at 2:00 PM
27,000+ plasmids, curated from Addgene's repository of 170,000+ constructs — spanning CRISPR, imaging and reporters, and gene expression — now available as ready-to-pipette Transfection-Ready DNA. And more to come!
https://twp.ai/4hvlat
#Plasmids #CRISPR
https://twp.ai/4hvlat
#Plasmids #CRISPR
The beauty of viral transfection in #avianretina 🐔
Here is a transfected #amacrinecell and some #photoreceptors in a cross-section of a #chick #retina.
Great image for #FluorescenceFriday
Sample from
@neurofishh.bsky.social
Green: Viral transfected retina; Red: DAPI for stratification
Here is a transfected #amacrinecell and some #photoreceptors in a cross-section of a #chick #retina.
Great image for #FluorescenceFriday
Sample from
@neurofishh.bsky.social
Green: Viral transfected retina; Red: DAPI for stratification
January 17, 2025 at 3:45 PM
The beauty of viral transfection in #avianretina 🐔
Here is a transfected #amacrinecell and some #photoreceptors in a cross-section of a #chick #retina.
Great image for #FluorescenceFriday
Sample from
@neurofishh.bsky.social
Green: Viral transfected retina; Red: DAPI for stratification
Here is a transfected #amacrinecell and some #photoreceptors in a cross-section of a #chick #retina.
Great image for #FluorescenceFriday
Sample from
@neurofishh.bsky.social
Green: Viral transfected retina; Red: DAPI for stratification
503 Service Temporarily Unavailable www.wiseguyreports.com/reports/lent...
Lentivirus Transfection Reagent Market | Size & Outlook 2035
The Lentivirus Transfection Reagent Market is expected to grow from 1,023 USD Million in 2025 to 2,500 USD Million by 2035. The Lentivirus Transfection Reagent Market CAGR (growth rate) is expected to...
www.wiseguyreports.com
September 30, 2026 at 6:53 AM
503 Service Temporarily Unavailable www.wiseguyreports.com/reports/lent...
“you can’t make this up” says account who regularly & infamously makes shit up
February 8, 2025 at 7:58 PM
“you can’t make this up” says account who regularly & infamously makes shit up
Sometimes you just don't want to deal with bacteria culture and prep - Addgene now delivers DNA ready to go! 🧬🚀
Check it out: www.addgene.org/dna-service/
Check it out: www.addgene.org/dna-service/
September 15, 2026 at 1:17 PM
Sometimes you just don't want to deal with bacteria culture and prep - Addgene now delivers DNA ready to go! 🧬🚀
Check it out: www.addgene.org/dna-service/
Check it out: www.addgene.org/dna-service/
Speaking as a scientist, I am confident that nature rejuvenates Tolkein elves due to airborne-virus-mediated transfection of AOX genes from plants.
October 7, 2024 at 10:18 PM
Speaking as a scientist, I am confident that nature rejuvenates Tolkein elves due to airborne-virus-mediated transfection of AOX genes from plants.
Not even sure I'm allowed to use transfection reagent anymore.
February 20, 2025 at 12:36 AM
Not even sure I'm allowed to use transfection reagent anymore.
An optimized macrophage-specific transfection strategy supports in vivo CRISPR editing of mRNAs to facilitate phagocytosis
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
January 15, 2025 at 10:54 PM
An optimized macrophage-specific transfection strategy supports in vivo CRISPR editing of mRNAs to facilitate phagocytosis
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
2) Transfection and testing for arbovirus inhibition of a mosquito-specific virus in Aedes aegypti and A. albopictus mosquitoes www.mdpi.com/2076-2607/9/...
🧵3/5
🧵3/5
Interactions of the Insect-Specific Palm Creek Virus with Zika and Chikungunya Viruses in Aedes Mosquitoes
Palm Creek virus (PCV) is an insect-specific flavivirus that can interfere with the replication of mosquito-borne flaviviruses in Culex mosquitoes, thereby potentially reducing disease transmission. W...
www.mdpi.com
March 3, 2025 at 12:59 PM
2) Transfection and testing for arbovirus inhibition of a mosquito-specific virus in Aedes aegypti and A. albopictus mosquitoes www.mdpi.com/2076-2607/9/...
🧵3/5
🧵3/5
Are you curious about animal origins? Want to perturb their genetics to find out more? Now it's a lot cheaper: the FUNR @frutag33.bsky.social Lab's effort to make transfection more accessible for choanos (& relatives) is up now! 🧬
A brief 🧵1/5
A brief 🧵1/5
March 15, 2026 at 10:35 PM
Are you curious about animal origins? Want to perturb their genetics to find out more? Now it's a lot cheaper: the FUNR @frutag33.bsky.social Lab's effort to make transfection more accessible for choanos (& relatives) is up now! 🧬
A brief 🧵1/5
A brief 🧵1/5
This was in the early 1990s.
I am not in neurosci or mol bio but my impression is that Axel was about the alpha-dogiest person in those fields, having developed euk. transfection methods.
He was notorious at Hopkins since he got his MD with the condition that he never practice on live patients.
I am not in neurosci or mol bio but my impression is that Axel was about the alpha-dogiest person in those fields, having developed euk. transfection methods.
He was notorious at Hopkins since he got his MD with the condition that he never practice on live patients.
February 26, 2026 at 4:48 PM
This was in the early 1990s.
I am not in neurosci or mol bio but my impression is that Axel was about the alpha-dogiest person in those fields, having developed euk. transfection methods.
He was notorious at Hopkins since he got his MD with the condition that he never practice on live patients.
I am not in neurosci or mol bio but my impression is that Axel was about the alpha-dogiest person in those fields, having developed euk. transfection methods.
He was notorious at Hopkins since he got his MD with the condition that he never practice on live patients.
We now offer Transfection-Ready DNA — NGS sequence-verified plasmid DNA from Addgene, ready to go for your next transfection. Available now: https://twp.ai/4hvlbn
#Plasmids #Transfection
#Plasmids #Transfection
September 18, 2026 at 2:00 PM
We now offer Transfection-Ready DNA — NGS sequence-verified plasmid DNA from Addgene, ready to go for your next transfection. Available now: https://twp.ai/4hvlbn
#Plasmids #Transfection
#Plasmids #Transfection
some of my cells finally recovered from transfection 🥹 so glad i don't have to repeat the whole thing
March 11, 2026 at 9:17 AM
some of my cells finally recovered from transfection 🥹 so glad i don't have to repeat the whole thing
What is Rearranged during Transfection?
By Dr. @ChristineBestv1 at our Targeted Therapies in Lung Cancer Event.
#RET #LungCancer #TargetedTherapies #RETFussion
By Dr. @ChristineBestv1 at our Targeted Therapies in Lung Cancer Event.
#RET #LungCancer #TargetedTherapies #RETFussion
March 15, 2025 at 6:51 PM
What is Rearranged during Transfection?
By Dr. @ChristineBestv1 at our Targeted Therapies in Lung Cancer Event.
#RET #LungCancer #TargetedTherapies #RETFussion
By Dr. @ChristineBestv1 at our Targeted Therapies in Lung Cancer Event.
#RET #LungCancer #TargetedTherapies #RETFussion
I'd probably make a good hermit.
People could deliver me a small portion of the month's harvest, and I could install Linux on their workstations, or teach the village children about gene transfection.
People could deliver me a small portion of the month's harvest, and I could install Linux on their workstations, or teach the village children about gene transfection.
Hitting a level of accumulated life stress that has me wishing I could server all ties and obligations.
Make a new life from scratch, or become some kind of mountain hermit or something.
Make a new life from scratch, or become some kind of mountain hermit or something.
January 14, 2026 at 1:22 PM
I'd probably make a good hermit.
People could deliver me a small portion of the month's harvest, and I could install Linux on their workstations, or teach the village children about gene transfection.
People could deliver me a small portion of the month's harvest, and I could install Linux on their workstations, or teach the village children about gene transfection.
we want to do some pilot experiments doing gene editing of a mammalian cell line using guide-plasmid transfection in cas9-expressing cells. do you have a favorite cell line that transfects easily?
pls re-ski
pls re-ski
March 20, 2026 at 7:07 PM
we want to do some pilot experiments doing gene editing of a mammalian cell line using guide-plasmid transfection in cas9-expressing cells. do you have a favorite cell line that transfects easily?
pls re-ski
pls re-ski