Spirochrome
@spirochrome.com
Our main focus is to offer innovative tools for live cell fluorescence imaging to the scientific community.
https://spirochrome.com/
https://spirochrome.com/
Reposted by Spirochrome
1/3 A few snapshots from the past intense days at the University of Helsinki for our FLIM and FCS course! We combined lectures, hands-on microscopy and analysis with plenty of questions and discussion. A fantastic group of researchers to learn with. 🔬
#FLIM #FCS #Microscopy
#FLIM #FCS #Microscopy
September 10, 2026 at 9:48 PM
1/3 A few snapshots from the past intense days at the University of Helsinki for our FLIM and FCS course! We combined lectures, hands-on microscopy and analysis with plenty of questions and discussion. A fantastic group of researchers to learn with. 🔬
#FLIM #FCS #Microscopy
#FLIM #FCS #Microscopy
Reposted by Spirochrome
Happy #FluorescenceFriday! This is the cortical actin cytoskeleton of a macrophage imaged using eSRRF and @spirochrome.com's SPY555-FastAct_X.
August 28, 2026 at 5:19 PM
Happy #FluorescenceFriday! This is the cortical actin cytoskeleton of a macrophage imaged using eSRRF and @spirochrome.com's SPY555-FastAct_X.
Spoiler: lots of 😍 SPY555-FastAct live cell movies in there.
Our June issue is here! rupress.org/jcb/issue/22...
The cover shows a maximum intensity projection from a confocal image stack showing an angiogenic sprout emerging from an engineered microvessel. From @lakynm.bsky.social et al. of the Matthew Kutys lab: rupress.org/jcb/article/...
#Cytoskeleton
The cover shows a maximum intensity projection from a confocal image stack showing an angiogenic sprout emerging from an engineered microvessel. From @lakynm.bsky.social et al. of the Matthew Kutys lab: rupress.org/jcb/article/...
#Cytoskeleton
June 1, 2026 at 4:31 PM
Spoiler: lots of 😍 SPY555-FastAct live cell movies in there.
Today is the Day, CenSpark650 is available!
Time to get those centrioles and cilia shining under the 🔬objective!
spirochrome.com/product/cens...
video: 20h imaging of primary cilium formation in a single RPE-1 cell stained with CenSpark650.
movie credits: @cepourroy.bsky.social
Time to get those centrioles and cilia shining under the 🔬objective!
spirochrome.com/product/cens...
video: 20h imaging of primary cilium formation in a single RPE-1 cell stained with CenSpark650.
movie credits: @cepourroy.bsky.social
June 1, 2026 at 2:43 PM
Today is the Day, CenSpark650 is available!
Time to get those centrioles and cilia shining under the 🔬objective!
spirochrome.com/product/cens...
video: 20h imaging of primary cilium formation in a single RPE-1 cell stained with CenSpark650.
movie credits: @cepourroy.bsky.social
Time to get those centrioles and cilia shining under the 🔬objective!
spirochrome.com/product/cens...
video: 20h imaging of primary cilium formation in a single RPE-1 cell stained with CenSpark650.
movie credits: @cepourroy.bsky.social
Reposted by Spirochrome
An amazing new tool to explore "non-models" is just about to open a new world of imagining possibilities....
📢On June 1st, we will be launching CenSpark650, the very first selective fluorescent probe for imaging centrioles and cilia in live cells.🔬 No transfection, zero genetic manipulation. Just Add & Image 1 hour later. Learn more: spirochrome.com/12-may-2026/
#Bioimaging
#Bioimaging
May 28, 2026 at 4:36 AM
An amazing new tool to explore "non-models" is just about to open a new world of imagining possibilities....
📢On June 1st, we will be launching CenSpark650, the very first selective fluorescent probe for imaging centrioles and cilia in live cells.🔬 No transfection, zero genetic manipulation. Just Add & Image 1 hour later. Learn more: spirochrome.com/12-may-2026/
#Bioimaging
#Bioimaging
May 27, 2026 at 3:23 PM
📢On June 1st, we will be launching CenSpark650, the very first selective fluorescent probe for imaging centrioles and cilia in live cells.🔬 No transfection, zero genetic manipulation. Just Add & Image 1 hour later. Learn more: spirochrome.com/12-may-2026/
#Bioimaging
#Bioimaging
Casually posting this one for no particular reason! #MayTheFourth
May 4, 2026 at 10:35 AM
Casually posting this one for no particular reason! #MayTheFourth
Reposted by Spirochrome
Overly proud that Katie Holden's image won Evident Microscopy's 6th Annual Image of the Year "Global Winner" award🏆
The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes.
evidentscientific.com/en/landing/i...
The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes.
evidentscientific.com/en/landing/i...
May 1, 2026 at 7:41 AM
Overly proud that Katie Holden's image won Evident Microscopy's 6th Annual Image of the Year "Global Winner" award🏆
The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes.
evidentscientific.com/en/landing/i...
The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes.
evidentscientific.com/en/landing/i...
Reposted by Spirochrome
#FluorescenceFriday material here!
Overly proud that Katie Holden's image won Evident Microscopy's 6th Annual Image of the Year "Global Winner" award🏆
The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes.
evidentscientific.com/en/landing/i...
The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes.
evidentscientific.com/en/landing/i...
May 1, 2026 at 8:08 AM
#FluorescenceFriday material here!
Overly proud that Katie Holden's image won Evident Microscopy's 6th Annual Image of the Year "Global Winner" award🏆
The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes.
evidentscientific.com/en/landing/i...
The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes.
evidentscientific.com/en/landing/i...
May 1, 2026 at 7:41 AM
Overly proud that Katie Holden's image won Evident Microscopy's 6th Annual Image of the Year "Global Winner" award🏆
The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes.
evidentscientific.com/en/landing/i...
The image shows Live iPSC-derived neurospheres. DNA (magenta) & MT (blue) were stained with our probes SiR-tubulin & SPY555-DNA probes.
evidentscientific.com/en/landing/i...
Reposted by Spirochrome
What if you got to work with some of your best friends on a science project? I can't publish how fun this was, but I can show you the data (🧵)! Last week, we posted our second neutrophil swarming paper to bioRxiv and I wanted to post my favorite videos here~
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
April 20, 2026 at 7:38 PM
What if you got to work with some of your best friends on a science project? I can't publish how fun this was, but I can show you the data (🧵)! Last week, we posted our second neutrophil swarming paper to bioRxiv and I wanted to post my favorite videos here~
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Mesmerizing light sheet imaging of neutrophils swarming at a yeast target!
The neutrophils were labeled with SPY650-DNA (yellow) and a calcium dye (blue).
This movie is part of the recent manuscript by @strickland-evelyn.bsky.social that we highly recommend reading!
www.biorxiv.org/content/10.6...
The neutrophils were labeled with SPY650-DNA (yellow) and a calcium dye (blue).
This movie is part of the recent manuscript by @strickland-evelyn.bsky.social that we highly recommend reading!
www.biorxiv.org/content/10.6...
April 21, 2026 at 2:04 PM
Mesmerizing light sheet imaging of neutrophils swarming at a yeast target!
The neutrophils were labeled with SPY650-DNA (yellow) and a calcium dye (blue).
This movie is part of the recent manuscript by @strickland-evelyn.bsky.social that we highly recommend reading!
www.biorxiv.org/content/10.6...
The neutrophils were labeled with SPY650-DNA (yellow) and a calcium dye (blue).
This movie is part of the recent manuscript by @strickland-evelyn.bsky.social that we highly recommend reading!
www.biorxiv.org/content/10.6...
What a probe! Congratulations @cepourroy.bsky.social!
We're happy to share that we re working hard on making CenSpark probes available to the centriole aficionados in the coming weeks!
We're happy to share that we re working hard on making CenSpark probes available to the centriole aficionados in the coming weeks!
CenSpark story is now published in @natchembio.nature.com. We engineered a dual-ligand fluorescent probe to selectively "light up" centrioles & cilia. Massive thank to Pierre Gönczy, @lreymond.bsky.social and @ghatzopoulos.bsky.social for the guidance. Check it out 👉: doi.org/10.1038/s415...
Development of the fluorescent probe CenSpark for labeling centrioles and cilia - Nature Chemical Biology
CenSpark is a dual-ligand fluorescent probe binding simultaneously inner and outer microtubule sites, a configuration unique to doublet and triplet microtubules, enabling selective live imaging of cen...
doi.org
April 16, 2026 at 8:25 PM
What a probe! Congratulations @cepourroy.bsky.social!
We're happy to share that we re working hard on making CenSpark probes available to the centriole aficionados in the coming weeks!
We're happy to share that we re working hard on making CenSpark probes available to the centriole aficionados in the coming weeks!
Organoids stained with Spirochrome probes for actin and DNA! 😍
Painted 100+ lung organoids for Easter, stained with live-cell Cell Tracker/ @spirochrome.com dyes, imaged at our @leicamicrosystems.bsky.social Viventis Deep light-sheet 🔬 at Charité (soon @Si-M - Der Simulierte Mensch) and visualized using inverted lookup tables designed by @christlet.bsky.social
April 8, 2026 at 8:12 AM
Organoids stained with Spirochrome probes for actin and DNA! 😍
Reposted by Spirochrome
See how PKMito dyes promote mitochondrial research😀
@spirochrome.com
@spirochrome.com
Our paper is now out in Science! Super excited to share our discovery that #mitochondria #pearling is the elusive mechanism driving the regular distribution and inheritance of #mtDNA nucleoids 🧬 [1/6]
April 4, 2026 at 9:16 AM
See how PKMito dyes promote mitochondrial research😀
@spirochrome.com
@spirochrome.com
Beware the SPY488-FastAct rollercoaster!
We teamed up with CSR Biotech and their incredible MI-SIM microscope to drive you through the intricate actin network of live COS7 cells stained with SPY488-FastAct.
Find out more about SPY488-FastAct: spirochrome.com/product/spy4...
We teamed up with CSR Biotech and their incredible MI-SIM microscope to drive you through the intricate actin network of live COS7 cells stained with SPY488-FastAct.
Find out more about SPY488-FastAct: spirochrome.com/product/spy4...
April 1, 2026 at 1:43 PM
Beware the SPY488-FastAct rollercoaster!
We teamed up with CSR Biotech and their incredible MI-SIM microscope to drive you through the intricate actin network of live COS7 cells stained with SPY488-FastAct.
Find out more about SPY488-FastAct: spirochrome.com/product/spy4...
We teamed up with CSR Biotech and their incredible MI-SIM microscope to drive you through the intricate actin network of live COS7 cells stained with SPY488-FastAct.
Find out more about SPY488-FastAct: spirochrome.com/product/spy4...
Reposted by Spirochrome
📢 Staining #Peroxisomes in live & fixed cells with Peroxi_SPY probes has never been so easy!
There is now a very detailed #JOVE video that explains it all step by step.
Video link: dx.doi.org/10.3791/6940...
Peroxi_SPY link: spirochrome.com/products/?_c...
🧪
There is now a very detailed #JOVE video that explains it all step by step.
Video link: dx.doi.org/10.3791/6940...
Peroxi_SPY link: spirochrome.com/products/?_c...
🧪
March 23, 2026 at 2:26 PM
📢 Staining #Peroxisomes in live & fixed cells with Peroxi_SPY probes has never been so easy!
There is now a very detailed #JOVE video that explains it all step by step.
Video link: dx.doi.org/10.3791/6940...
Peroxi_SPY link: spirochrome.com/products/?_c...
🧪
There is now a very detailed #JOVE video that explains it all step by step.
Video link: dx.doi.org/10.3791/6940...
Peroxi_SPY link: spirochrome.com/products/?_c...
🧪
📢 Staining #Peroxisomes in live & fixed cells with Peroxi_SPY probes has never been so easy!
There is now a very detailed #JOVE video that explains it all step by step.
Video link: dx.doi.org/10.3791/6940...
Peroxi_SPY link: spirochrome.com/products/?_c...
🧪
There is now a very detailed #JOVE video that explains it all step by step.
Video link: dx.doi.org/10.3791/6940...
Peroxi_SPY link: spirochrome.com/products/?_c...
🧪
March 23, 2026 at 2:26 PM
📢 Staining #Peroxisomes in live & fixed cells with Peroxi_SPY probes has never been so easy!
There is now a very detailed #JOVE video that explains it all step by step.
Video link: dx.doi.org/10.3791/6940...
Peroxi_SPY link: spirochrome.com/products/?_c...
🧪
There is now a very detailed #JOVE video that explains it all step by step.
Video link: dx.doi.org/10.3791/6940...
Peroxi_SPY link: spirochrome.com/products/?_c...
🧪
🤩 Another amazing example of U-ExM actin staining with HAK-actin by @dudinlab.bsky.social et. al.
At this high resolution, we also caught something new: Using the new #HAK-Actin #UExM probe, we saw that
#Actin accumulates at the furrow base before compartments fully seal ➡️ they're still open at the bottom.
➡️ The #multicellular epithelium-like stage is shorter than we previously proposed.
#Actin accumulates at the furrow base before compartments fully seal ➡️ they're still open at the bottom.
➡️ The #multicellular epithelium-like stage is shorter than we previously proposed.
March 18, 2026 at 12:28 PM
🤩 Another amazing example of U-ExM actin staining with HAK-actin by @dudinlab.bsky.social et. al.
Reposted by Spirochrome
An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™.
These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green).
Movie from preprint www.biorxiv.org/content/10.6...
🧪
These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green).
Movie from preprint www.biorxiv.org/content/10.6...
🧪
March 16, 2026 at 11:43 AM
An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™.
These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green).
Movie from preprint www.biorxiv.org/content/10.6...
🧪
These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green).
Movie from preprint www.biorxiv.org/content/10.6...
🧪
An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™.
These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green).
Movie from preprint www.biorxiv.org/content/10.6...
🧪
These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green).
Movie from preprint www.biorxiv.org/content/10.6...
🧪
March 16, 2026 at 11:43 AM
An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™.
These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green).
Movie from preprint www.biorxiv.org/content/10.6...
🧪
These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green).
Movie from preprint www.biorxiv.org/content/10.6...
🧪
Reposted by Spirochrome
🧵 1/5 A new BioRxiv preprint reveals that membrane tension acts as a mechanical gate for lung cell fate during development! 🫁
Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT)
Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology
Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT)
Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology
March 10, 2026 at 2:14 PM
🧵 1/5 A new BioRxiv preprint reveals that membrane tension acts as a mechanical gate for lung cell fate during development! 🫁
Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT)
Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology
Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT)
Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology
🧵 1/5 A new BioRxiv preprint reveals that membrane tension acts as a mechanical gate for lung cell fate during development! 🫁
Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT)
Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology
Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT)
Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology
March 10, 2026 at 2:14 PM
🧵 1/5 A new BioRxiv preprint reveals that membrane tension acts as a mechanical gate for lung cell fate during development! 🫁
Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT)
Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology
Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT)
Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis. 👇 #Mechanobiology
Reposted by Spirochrome
🟢FastAct™ goes green! 🟢🔬
We launch SPY488-FastAct™ a green channel live cell actin probe
SPY488-FastAct allows imaging F-actin in live and fixed cells
Compatible with SPY555 and SPY650 for easy 3 color imaging.
spirochrome.com/product/spy4...
available at our distributors in a couple of days. 🧪
We launch SPY488-FastAct™ a green channel live cell actin probe
SPY488-FastAct allows imaging F-actin in live and fixed cells
Compatible with SPY555 and SPY650 for easy 3 color imaging.
spirochrome.com/product/spy4...
available at our distributors in a couple of days. 🧪
March 3, 2026 at 12:18 PM
🟢FastAct™ goes green! 🟢🔬
We launch SPY488-FastAct™ a green channel live cell actin probe
SPY488-FastAct allows imaging F-actin in live and fixed cells
Compatible with SPY555 and SPY650 for easy 3 color imaging.
spirochrome.com/product/spy4...
available at our distributors in a couple of days. 🧪
We launch SPY488-FastAct™ a green channel live cell actin probe
SPY488-FastAct allows imaging F-actin in live and fixed cells
Compatible with SPY555 and SPY650 for easy 3 color imaging.
spirochrome.com/product/spy4...
available at our distributors in a couple of days. 🧪
🟢FastAct™ goes green! 🟢🔬
We launch SPY488-FastAct™ a green channel live cell actin probe
SPY488-FastAct allows imaging F-actin in live and fixed cells
Compatible with SPY555 and SPY650 for easy 3 color imaging.
spirochrome.com/product/spy4...
available at our distributors in a couple of days. 🧪
We launch SPY488-FastAct™ a green channel live cell actin probe
SPY488-FastAct allows imaging F-actin in live and fixed cells
Compatible with SPY555 and SPY650 for easy 3 color imaging.
spirochrome.com/product/spy4...
available at our distributors in a couple of days. 🧪
March 3, 2026 at 12:18 PM
🟢FastAct™ goes green! 🟢🔬
We launch SPY488-FastAct™ a green channel live cell actin probe
SPY488-FastAct allows imaging F-actin in live and fixed cells
Compatible with SPY555 and SPY650 for easy 3 color imaging.
spirochrome.com/product/spy4...
available at our distributors in a couple of days. 🧪
We launch SPY488-FastAct™ a green channel live cell actin probe
SPY488-FastAct allows imaging F-actin in live and fixed cells
Compatible with SPY555 and SPY650 for easy 3 color imaging.
spirochrome.com/product/spy4...
available at our distributors in a couple of days. 🧪