Cell-free synthetic biology aims at the targeted replication, design, and modification of life processes in open systems by breaking free of constrain…

Cell-free is a powerful platform to unravel biological complexity. Here, the authors used active learning to optimize buffer composition, boosting mRNA 20-fold, enabling cell-free transcriptomics and ...

The realization of ribosome biogenesis in vitro is recognized as a critical milestone in the creation of artificial cells. Here, the authors developed a method to detect newly synthesized ribosomes, w...

Cell-free gene expression (CFE) systems empower synthetic biologists to build biological molecules and processes outside of living intact cells. The foundational principle is that precise, complex bio...

Cell-free protein synthesis is a platform for streamlined production of macromolecules. Recently, several proteins with pharmaceutical relevance were …

Cell-free protein synthesis (CFPS) can permit workflow time savings and high throughput, while avoiding several limitations associated with in vivo protein expression systems. Learn more about how you...

Throughout history, we have looked to nature to discover and copy pharmaceutical solutions to prevent and heal diseases. Due to the advances in metabolic engineering and the production of pharmaceutical proteins in different host cells, we have moved from mimicking nature to the delicate engineering of cells and proteins. We can now produce novel drug molecules, which are fusions of small chemical drugs and proteins. Currently we are at the brink of yet another step to venture beyond nature’s border with the use of unnatural amino acids and manufacturing without the use of living cells using cell-free systems. In this review, we summarize the progress and limitations of the last decades in the development of pharmaceutical protein development, production in cells, and cell-free systems. We also discuss possible future directions of the field.

The cell-free protein synthesis (CFPS) system, as a technical core of synthetic biology, can simulate the transcription and translation process in an …

Cell-free protein synthesis (CFPS) serves as an innovation booster in the field of biocatalysis. By integrating CFPS into the design and development o…

A team led by Francesca Demichelis and Misha Beltran reports the development of the NEMO, which assesses DNA methylation at selected regions in cell-free DNA from liquid biopsies to provide a non-inva...

The ability to evaluate the effect of different solubility tags on protein expression and the impact of their removal can be done simultaneously on eProtein Discovery. Data in this application note va...