YouTube video by Kristiane

Plasma membrane integrity is vital for nearly all aspects of cell functioning1. Mechanical forces can cause plasma membrane damage2, but it is not known whether there are large molecules that regulate plasma membrane integrity under mechanical strain. Here we constructed a 384-well cellular stretch system that delivers precise, reproducible strain to cultured cells. Using the system, we screened 10,843 siRNAs targeting 2,726 multi-pass transmembrane proteins for strain-induced membrane permeability changes.

Hurricane Ida made landfall in Louisiana as a Category 4 hurricane on August 29, 2021, producing strong winds, storm surges, and intense rainfall that devastated the Gulf Coast1. As it moved inland (SI Fig. S1), the storm’s remnants and seven tornadoes that struck Philadelphia between September 1–2 triggered unprecedented discharge and a 1-in-100-year flood along the Schuylkill River, causing widespread damage2 (SI Table S2). Extreme hydrometeorological events (EHMEs) like Ida already cause average global losses of $136.7 billion annually (2003–2022)3, surging to over $320 billion in 2024 alone4. Rain-driven floods are projected to intensify as climate extremes intensify and urbanization expands, thereby constraining rivers and altering floodplain dynamics5,6. In tidally influenced rivers, sea-level rise (SLR) and stronger tropical cyclones further amplify flood severity and human vulnerability7,8. Currently, 13.3% of the U.S. population—over 40 million people—is exposed to a 1-in-100-year flood9, yet future flood risks in urban and coastal corridors remain poorly quantified10,11, relying on coarse models that obscure critical compound drivers and limit effective mitigation in densely populated metropolitan regions12. Philadelphia exemplifies this challenge: as the second-most populous city on the U.S. East Coast (1.5M), bounded by the Delaware River to the east and bisected by the Lower Schuylkill River...

Plasma membrane integrity is vital for nearly all aspects of cell functioning1. Mechanical forces can cause plasma membrane damage2, but it is not known whether there are large molecules that regulate plasma membrane integrity under mechanical strain. Here we constructed...

Damage2はHeavyocityのエピックパーカッション音源です。 名前の通り破壊力のあるパーカッションを収録した音源で、エピックパーカッションの定番音源でもあります。 映画音楽やシネマティックな雰囲気を出したいならDamage2のよう

The integrity of the plasma membrane is vital for nearly all aspects of cell functioning1. Mechanical forces can cause plasma membrane damage2, but it is unclear whether there are large molecules that regulate the integrity of the plasma membrane under mechanical strain. Here we constructed a 384-well cellular-stretch system that delivers precise, reproducible strain to cultured cells. Using the system, we screened 10,843 small interfering RNAs (siRNAs) targeting 2,726 multipass transmembrane proteins for strain-induced membrane permeability changes. The screen identified NINJ1—a protein that was recently proposed to regulate pyroptosis and other lytic cell death3,4—as the top hit. We demonstrate that NINJ1 is a critical regulator of mechanical-strain-induced plasma membrane rupture (PMR), without the need for stimulating any cell death programs. NINJ1 levels on the plasma membrane are inversely correlated with the amount of force required to rupture the membrane. In the pyroptosis con