www.nature.com/articles/s41...
Chromatin decompaction within the nucleus increases plasma membrane tension promoting NETosis execution, independently of transcription
Chromatin decompaction within the nucleus increases plasma membrane tension promoting NETosis execution, independently of transcription
August 20, 2026 at 6:32 AM
www.nature.com/articles/s41...
Chromatin decompaction within the nucleus increases plasma membrane tension promoting NETosis execution, independently of transcription
Chromatin decompaction within the nucleus increases plasma membrane tension promoting NETosis execution, independently of transcription
Nowadays, animals till the seafloor. They make holes, eat the organic matter buried there, and flush the sediment with water. But this system had to evolve through the Phanerozoic into its current form, with a few ups and down along the way. 🧪⚒️
Link: www.science.org/doi/10.1126/...
Link: www.science.org/doi/10.1126/...
August 4, 2025 at 7:03 AM
Nowadays, animals till the seafloor. They make holes, eat the organic matter buried there, and flush the sediment with water. But this system had to evolve through the Phanerozoic into its current form, with a few ups and down along the way. 🧪⚒️
Link: www.science.org/doi/10.1126/...
Link: www.science.org/doi/10.1126/...
Turns out the way DNA is packed inside the nucleus can tune the mechanics of the entire cell!
Super excited that our first paper is out in Nature Communications! www.nature.com/articles/s41... !
Super excited that our first paper is out in Nature Communications! www.nature.com/articles/s41... !
Chromatin decompaction within the nucleus increases plasma membrane tension promoting NETosis execution, independently of transcription - Nature Communications
Chromatin organizes nuclear DNA, but whether it actively regulates whole-cell mechanics remains unknown. Here, we used NETosis to show that chromatin decompaction within the nucleus increases plasma membrane tension and cell volume, establishing chromatin as a regulator of cell mechanics.
www.nature.com
August 20, 2026 at 5:20 PM
Turns out the way DNA is packed inside the nucleus can tune the mechanics of the entire cell!
Super excited that our first paper is out in Nature Communications! www.nature.com/articles/s41... !
Super excited that our first paper is out in Nature Communications! www.nature.com/articles/s41... !
Our new preprint is out!
We combined single-nucleosome imaging and 3D-SIM to reveal:
🔹 Euchromatin forms condensed domains, not open fibers
🔹Cohesin loss increases nucleosome mobility without decompaction
🔹Cohesin prevents neighboring domain mixing
Full story & movies👇
We combined single-nucleosome imaging and 3D-SIM to reveal:
🔹 Euchromatin forms condensed domains, not open fibers
🔹Cohesin loss increases nucleosome mobility without decompaction
🔹Cohesin prevents neighboring domain mixing
Full story & movies👇
Is euchromatin really “open”? Our new study @bioRxiv suggests otherwise. Using super-resolution imaging @shiori-iida.bsky.social @masaashimazoe.bsky.social reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
August 28, 2025 at 3:11 PM
Our new preprint is out!
We combined single-nucleosome imaging and 3D-SIM to reveal:
🔹 Euchromatin forms condensed domains, not open fibers
🔹Cohesin loss increases nucleosome mobility without decompaction
🔹Cohesin prevents neighboring domain mixing
Full story & movies👇
We combined single-nucleosome imaging and 3D-SIM to reveal:
🔹 Euchromatin forms condensed domains, not open fibers
🔹Cohesin loss increases nucleosome mobility without decompaction
🔹Cohesin prevents neighboring domain mixing
Full story & movies👇
interesting paper from the group of @arpcomplex.bsky.social on how chromatin decompaction modulates nuclear shape and actin dynamics for T-cell function doi.org/10.1091/mbc....
July 9, 2026 at 5:59 AM
interesting paper from the group of @arpcomplex.bsky.social on how chromatin decompaction modulates nuclear shape and actin dynamics for T-cell function doi.org/10.1091/mbc....
We look forward to hearing feedback from the community on our #preprint where we used #NETosis as a platform to show that chromatin decompaction inside the nucleus directly regulates cell mechanics. This work identifies long-range biophysical impacts of chromatin in mammalian cells.
Chromatin is a long-range force generator that regulates plasma membrane tension and cell integrity independently of gene expression https://www.biorxiv.org/content/10.1101/2025.10.02.680155v1
October 6, 2025 at 10:18 AM
So, is it compaction or decompaction? Here is a notable alternative hypothesis: "Our data suggest that the process of..loop extrusion per se is not required for enhancer function..Rather, it is the chromatin compaction brought about by loop extrusion that may be the important factor to consider."
Plenty of work required to rigorously test the proposed hypothesis but my initial thoughts are this perspective by Adrian Bird in @cp-molcell.bsky.social may well be the most significant conceptual advance in the 3D genome organisation field in years:
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Cohesin as an essential disruptor of chromosome organization
Cohesin is a multi-subunit molecular machine that is able to create lateral chromatin loops within a linear chromosome fiber. Despite intense study, a…
www.sciencedirect.com
March 26, 2025 at 4:01 PM
So, is it compaction or decompaction? Here is a notable alternative hypothesis: "Our data suggest that the process of..loop extrusion per se is not required for enhancer function..Rather, it is the chromatin compaction brought about by loop extrusion that may be the important factor to consider."
from our 'older' paper Baarlink et al. 2017, live imaging of a dividing mouse fibroblast stably expressing a shuttling chromobody detecting actin - showing nuclear actin filaments during postmitotic nuclear expansion and chromatin decompaction as well as simultaneous membrane dynamics
June 27, 2025 at 11:07 AM
from our 'older' paper Baarlink et al. 2017, live imaging of a dividing mouse fibroblast stably expressing a shuttling chromobody detecting actin - showing nuclear actin filaments during postmitotic nuclear expansion and chromatin decompaction as well as simultaneous membrane dynamics
Telomeres stall DNA loop extrusion by condensin
Telomeres stall DNA loop extrusion by condensin
Analikwu et al. show that dense linear arrays of the telomeric protein Rap1 on DNA block DNA loop extrusion by condensin. Rap1-induced stalling depends on array length and protein density and leads to a local chromatin decompaction during anaphase, revealing how protein arrays can regulate SMC-driven genome organization.
dlvr.it
July 2, 2025 at 7:50 PM
Telomeres stall DNA loop extrusion by condensin
Time-Resolved Single-Molecule FRET Reveals Length-Dependent Nucleosome Decompaction by Poly(ADP-ribose) https://www.biorxiv.org/content/10.64898/2026.02.25.707951v1
February 27, 2026 at 7:49 PM
Time-Resolved Single-Molecule FRET Reveals Length-Dependent Nucleosome Decompaction by Poly(ADP-ribose) https://www.biorxiv.org/content/10.64898/2026.02.25.707951v1
3/Chromatin is compacted in apoptosis, might this sequester fragments? Histone deacetylation, which drives compaction in living cells, has been observed in apoptosis, but a role in apoptotic compaction was unknown. We inhibited apoptotic deacetylation which resulted in drastic chromatin decompaction
February 25, 2026 at 9:45 AM
3/Chromatin is compacted in apoptosis, might this sequester fragments? Histone deacetylation, which drives compaction in living cells, has been observed in apoptosis, but a role in apoptotic compaction was unknown. We inhibited apoptotic deacetylation which resulted in drastic chromatin decompaction
Winter improvements: rough aerification. Followed by TF seed. #golf #golfcourse #golfcoursemaintenance #agronomy #decompaction #teamworkmakesthedreamwork
February 27, 2025 at 7:21 PM
Winter improvements: rough aerification. Followed by TF seed. #golf #golfcourse #golfcoursemaintenance #agronomy #decompaction #teamworkmakesthedreamwork
Myelin Decompaction in Mice Given Anesthetics during Magnetic Resonance Imaging https://www.biorxiv.org/content/10.1101/2025.07.29.667499v1
August 1, 2025 at 6:18 PM
Myelin Decompaction in Mice Given Anesthetics during Magnetic Resonance Imaging https://www.biorxiv.org/content/10.1101/2025.07.29.667499v1
If that happens, it could suggest a second mechanism trying to mitigate the effect of decompaction at short time scales
February 14, 2025 at 4:36 PM
If that happens, it could suggest a second mechanism trying to mitigate the effect of decompaction at short time scales
New Online! Histone H1 deamidation regulates DNA double-strand break repair
Histone H1 deamidation regulates DNA double-strand break repair
Nature Reviews Molecular Cell Biology, Published online: 13 May 2025; doi:10.1038/s41580-025-00860-1Repair of DNA double-strand breaks requires chromatin opening; however, the mechanisms involved were unclear. This work shows that sequential histone deamidation and acetylation modifications induce chromatin decompaction by reducing positive charge at the DNA–nucleosome interface.
bit.ly
May 13, 2025 at 11:56 AM
New Online! Histone H1 deamidation regulates DNA double-strand break repair
ICYMI: New Online! Histone H1 deamidation regulates DNA double-strand break repair
Histone H1 deamidation regulates DNA double-strand break repair
Nature Reviews Molecular Cell Biology, Published online: 13 May 2025; doi:10.1038/s41580-025-00860-1Repair of DNA double-strand breaks requires chromatin opening; however, the mechanisms involved were unclear. This work shows that sequential histone deamidation and acetylation modifications induce chromatin decompaction by reducing positive charge at the DNA–nucleosome interface.
bit.ly
May 16, 2025 at 11:57 AM
ICYMI: New Online! Histone H1 deamidation regulates DNA double-strand break repair
We measured single molecule ssDNA binding by SSB proteins from different systems, E. coli SSB, T4 gene 32, and LINE-1 ORF1p. All showed surprising initial compaction then decompaction. This allows them to dissociate rapidly in response to crowding, as we show in QRB Discovery doi.org/10.1017/qrd....
Diverse single-stranded nucleic acid binding proteins enable both stable protection and rapid exchange required for biological function | QRB Discovery | Cambridge Core
Diverse single-stranded nucleic acid binding proteins enable both stable protection and rapid exchange required for biological function - Volume 6
doi.org
January 22, 2025 at 11:05 PM
We measured single molecule ssDNA binding by SSB proteins from different systems, E. coli SSB, T4 gene 32, and LINE-1 ORF1p. All showed surprising initial compaction then decompaction. This allows them to dissociate rapidly in response to crowding, as we show in QRB Discovery doi.org/10.1017/qrd....
New from MBoC:
Cytoplasmic RNAs in Xenopus laevis eggs help drive rapid pronucleus assembly by promoting chromatin decompaction and nuclear growth after fertilization.
https://www.molbiolcell.org/doi/10.1091/mbc.E25-09-0440
Cytoplasmic RNAs in Xenopus laevis eggs help drive rapid pronucleus assembly by promoting chromatin decompaction and nuclear growth after fertilization.
https://www.molbiolcell.org/doi/10.1091/mbc.E25-09-0440
March 16, 2026 at 11:25 AM
New from MBoC:
Cytoplasmic RNAs in Xenopus laevis eggs help drive rapid pronucleus assembly by promoting chromatin decompaction and nuclear growth after fertilization.
https://www.molbiolcell.org/doi/10.1091/mbc.E25-09-0440
Cytoplasmic RNAs in Xenopus laevis eggs help drive rapid pronucleus assembly by promoting chromatin decompaction and nuclear growth after fertilization.
https://www.molbiolcell.org/doi/10.1091/mbc.E25-09-0440
8/These charge manipulations also affected chromatin compaction in apoptotic cells: In the presence of positive nanobodies, chromatin compacted despite blocked histone deacetylation; while the negative nanobody caused the same decompaction phenotype as HDAC inhibition
February 25, 2026 at 9:45 AM
8/These charge manipulations also affected chromatin compaction in apoptotic cells: In the presence of positive nanobodies, chromatin compacted despite blocked histone deacetylation; while the negative nanobody caused the same decompaction phenotype as HDAC inhibition
The authors included RRIDs in their None paper! RRIDs improve reproducibility in scientific research. #OpenScience #reproducibility #methodsmatter
Daxx mediated histone H3.3 deposition on HSV-1 DNA restricts genome decompaction and the progression of immediate-early transcription
doi.org
September 7, 2025 at 7:03 AM
The authors included RRIDs in their None paper! RRIDs improve reproducibility in scientific research. #OpenScience #reproducibility #methodsmatter
Li, Y. R., Ling, L. B., Chao, A., Fugmann, S. D., Yang, S. Y. (2024). Transient chromatin decompaction at the start of D. melanogaster male embryonic germline development. Life science alliance, 7(10) www.life-science-alliance.org/content/7/10...
August 26, 2025 at 8:14 PM
Li, Y. R., Ling, L. B., Chao, A., Fugmann, S. D., Yang, S. Y. (2024). Transient chromatin decompaction at the start of D. melanogaster male embryonic germline development. Life science alliance, 7(10) www.life-science-alliance.org/content/7/10...
This fits well with your data: the increased inter-probe distances could reflect deformation/elongation of a TAD rather than uniform decompaction. Hi-C data (Fig. 8a) show that short-distance contacts decrease and long-distance contacts increase after cohesin loss. Thanks for pointing this out! 2/2
September 11, 2026 at 1:28 PM
This fits well with your data: the increased inter-probe distances could reflect deformation/elongation of a TAD rather than uniform decompaction. Hi-C data (Fig. 8a) show that short-distance contacts decrease and long-distance contacts increase after cohesin loss. Thanks for pointing this out! 2/2
I run multiple agents backed by OpenClaw for my yardwork. One on the lawnmower, one on the leaf blower, one on the edger and multiple doing soil decompaction and mulching
What previously took a team of 3 underpaid workers 1 hour now takes 5 minutes, but I did run over my dog and my wife lost a toe.
What previously took a team of 3 underpaid workers 1 hour now takes 5 minutes, but I did run over my dog and my wife lost a toe.
March 10, 2026 at 5:54 PM
I run multiple agents backed by OpenClaw for my yardwork. One on the lawnmower, one on the leaf blower, one on the edger and multiple doing soil decompaction and mulching
What previously took a team of 3 underpaid workers 1 hour now takes 5 minutes, but I did run over my dog and my wife lost a toe.
What previously took a team of 3 underpaid workers 1 hour now takes 5 minutes, but I did run over my dog and my wife lost a toe.