#gammaH2AX
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alicefrolov.bsky.social
New Visualization: Age-Dependent Slowing of Double-Strand Break Repair Foci Resolution

I am sharing a figure developed for pedagogical and hypothesis-framing purposes

#DNArepair #DoubleStrandBreaks #gammaH2AX #Radiobiology #AgingBiology #GenomicInstability #NHEJ
New Visualization: Age-Dependent Slowing of Double-Strand Break Repair Foci Resolution I am sharing a figure developed for pedagogical and hypothesis-framing purposes, titled "Age-Dependent Slowing of Double-Strand Break Repair Foci Resolution," with the subtitle "Simulated γH2AX foci clearance kinetics after 2 Gy ionizing radiation across donor age cohorts and repair pathways." Important caveat up front: the data plotted here are simulated. The curves do not derive from a single empirical dataset but are constructed to illustrate a well-established, literature-grounded trend regarding the attenuation of DSB repair capacity with advancing donor age. The multiline plot tracks residual γH2AX foci per nucleus as a function of post-irradiation time (0–48 h) following a 2 Gy dose. Cohorts are stratified by donor age (young, middle-aged, and aged), with parallel decompositions approximating the relative contributions of non-homologous end joining (NHEJ) and homologous recombination (HR). The simulated kinetics reproduce the canonical biphasic clearance profile: a rapid initial decline dominated by NHEJ-mediated rejoining within the first several hours, followed by a slower resolution phase reflecting HR processing of complex or clustered lesions. The salient qualitative feature is the progressive rightward shift and elevated residual foci plateau in older cohorts, consistent with reported declines in DNA-PKcs activity, chromatin remodeling efficiency, and 53BP1/BRCA1 pathway coordination during aging. The persistence of unresolved foci at 24–48 h in the aged cohort is intended to represent the accumulation of unrepaired or misrepaired breaks associated with senescence-associated genomic instability. I welcome methodological critique, particularly regarding parameterization of the HR/NHEJ balance and whether the assumed rate constants adequately capture reported interindividual variability.
August 7, 2026 at 5:00 PM
bethcimini.bsky.social
Poll particularly for biologists, esp @CellProfiler users!
Say you have an experiment where you're imaging Hoechst, anti-gammaH2AX with an Alexa 488 2ndary, and TexasRed-Phalloidin. What would you name your channels on the microscope/your analysis workflow?
November 10, 2024 at 5:47 PM
fly-broadcast.bsky.social
Global gammaH2AX phosphorylation in Drosophila is reversed by the phosphatase Mts
#Drosophila
Global gammaH2AX phosphorylation in Drosophila is reversed by the phosphatase Mts #Drosophila
PubMed link
pubmed.ncbi.nlm.nih.gov
March 7, 2025 at 11:23 AM
biorxivpreprint.bsky.social
Dihydromyricetin alleviated the damage of hypoxia-induced mouse neurons by reducing ROS levels and inhibiting the expression of PAR and gammaH2AX https://www.biorxiv.org/content/10.1101/2024.07.07.602423v1
Dihydromyricetin alleviated the damage of hypoxia-induced mouse neurons by reducing ROS levels and inhibiting the expression of PAR and gammaH2AX https://www.biorxiv.org/content/10.1101/2024.07.07.602423v1
Objective: To investigate the effect of dihydromyricetin on hypoxia-induced neurons to understand th
www.biorxiv.org
July 11, 2024 at 12:16 AM
biorxiv-neursci.bsky.social
Dihydromyricetin alleviated the damage of hypoxia-induced mouse neurons by reducing ROS levels and inhibiting the expression of PAR and gammaH2AX https://www.biorxiv.org/content/10.1101/2024.07.07.602423v1
Dihydromyricetin alleviated the damage of hypoxia-induced mouse neurons by reducing ROS levels and inhibiting the expression of PAR and gammaH2AX https://www.biorxiv.org/content/10.1101/2024.07.07.602423v1
Objective: To investigate the effect of dihydromyricetin on hypoxia-induced neurons to understand th
www.biorxiv.org
July 11, 2024 at 12:16 AM
interactivefly.bsky.social
Apostolou, Z., Krause, S., Becker, P. B. (2025). Global gammaH2AX phosphorylation in Drosophila is reversed by the phosphatase MtsMicroPubl Biol, 2025 www.micropublication.org/journals/bio...
April 30, 2026 at 5:50 PM