#ino80
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genecenter-lmu.bsky.social
📣 Congrats to the Stigler lab on their new publication! 👏 🥂
Their findings provide fascinating mechanistic insights into how the chromatin remodeler INO80 searches DNA and establishes chromatin organization at promoters and replication origins.
Find the details here:
doi.org/10.1038/s414...
Single-molecule imaging reveals DNA shape read-out by the INO80 chromatin remodeler - Nature Communications
How chromatin remodelers regulate nucleosome positioning remains poorly understood. Here, the authors use single-molecule fluorescence microscopy to reveal how read-out of DNA shape features regulates...
doi.org
September 30, 2026 at 7:20 AM
biorxiv-biophys.bsky.social
INO80 rapidly shuttles nucleosomes between chromatin barriers https://www.biorxiv.org/content/10.64898/2026.09.20.752975v1
September 22, 2026 at 6:46 PM
biorxivpreprint.bsky.social
INO80 rapidly shuttles nucleosomes between chromatin barriers https://www.biorxiv.org/content/10.64898/2026.09.20.752975v1
September 22, 2026 at 6:46 PM
hopfnerlab.bsky.social
🧵 We’re excited to share our new bioRxiv preprint on how INO80 reads promoter-proximal DNA sequence features to position +1 nucleosomes 🧬
Amazing work by Mariia, Annika, and others! 🤩
🔗 www.biorxiv.org/content/10.6...
#ChromatinBiology #StructuralBiology #Nucleosome #INO80
September 2, 2026 at 5:44 AM
hopfnerlab.bsky.social
2/4
Using cryo-EM and biochemical approaches, we characterise a novel INO80 binding mode (“Off-state”) on a -competent state when oriented toward the gene body, but an inactive Off-state when oriented toward the promoter.
September 2, 2026 at 5:44 AM
hopfnerlab.bsky.social
4/4
Together, our findings show how DNA sequence can influence both INO80 binding mode and remodelling directionality, providing a mechanism for sequence-driven +1 nucleosome positioning.
📄 Curious to learn more? Check out the preprint:
🔗 www.biorxiv.org/content/10.6...
www.biorxiv.org
September 2, 2026 at 5:44 AM
cryoempapers.bsky.social
ATP-dependent reorientation on a nucleosome primes INO80 for DNA translocation www.biorxiv.org/content/10.64898/2026.08.11.744281v1 #cryoem
August 15, 2026 at 7:10 AM
biorxivpreprint.bsky.social
ATP-dependent reorientation on a nucleosome primes INO80 for DNA translocation https://www.biorxiv.org/content/10.64898/2026.08.11.744281v1
August 15, 2026 at 4:46 AM
biorxiv-biophys.bsky.social
ATP-dependent reorientation on a nucleosome primes INO80 for DNA translocation https://www.biorxiv.org/content/10.64898/2026.08.11.744281v1
August 15, 2026 at 4:46 AM
biorxiv-biochem.bsky.social
Sequence-dependent binding modes of INO80 control +1 nucleosome positioning https://www.biorxiv.org/content/10.64898/2026.08.12.744421v1
August 13, 2026 at 9:46 PM
biorxivpreprint.bsky.social
Sequence-dependent binding modes of INO80 control +1 nucleosome positioning https://www.biorxiv.org/content/10.64898/2026.08.12.744421v1
August 13, 2026 at 9:46 PM
proteinimaging.bsky.social
Here you can see a cryoEM structure of the DeltaArp8 INO80 bound to S.c 0/40 nucleosome (PDB code: 9CAU)

Rendering by Francisco J. Enguita made with #ProteinImager

3dproteinimaging.com/protein-imag...

#SciArt #molecularart #nucleosome #saccharomyces #ino80 #cryoem
February 28, 2026 at 11:01 AM
hopfnerlab.bsky.social
We are excited to share our latest cryo-EM study of the human INO80 remodeler, now featured as a Breakthrough article in NAR @narjournal.bsky.social Check it out here and a big shoutout to @priyanka-pdm.bsky.social and Manmohan et al. for their contributions! 🧬
March 6, 2026 at 10:25 AM
hopfnerlab.bsky.social
Check out our paper on nucleosome positioning by our favorite remodeler INO80 in a collaboration with the Bartholomew Lab, finally out in MolCell 🧬

kwnsfk27.r.eu-west-1.awstrack.me/L0/https:%2F...
November 27, 2025 at 11:47 AM
hopfnerlab.bsky.social
Check out our pre-print on how the human INO80 complex acts on nucleosomes and hexasomes: PostDocs Priyanka & Manmohan et al. solved structures of INO80 bound to several substrates and completed missing questions about similarities of modes of actions across species🧬 www.biorxiv.org/content/10.1...
Recognition and remodelling of nucleosomes and hexasomes by the human INO80 complex
The ATP-dependent INO80 chromatin remodeller slides and repositions nucleosomes to shape and maintain chromatin around gene regulatory elements and replication origins. Recent work uncovered capabilit...
www.biorxiv.org
August 27, 2025 at 12:37 PM
genecenter-lmu.bsky.social
📣Publication alert!
Collaboration between Bartholomew lab and @hopfnerlab.bsky.social shows that INO80 positions nucleosomes at specific genomic sites by sensing intranucleosomal DNA shape and stalling at rigid DNA via its Arp5 subunit.
authors.elsevier.com/c/1m9kd3vVUP...
@mdanderson.bsky.social
authors.elsevier.com
November 27, 2025 at 11:23 AM
theplantcell.bsky.social
FVE and HDA9 form a complex to promote thermomorphogenesis by reducing H2A.Z deposition through histone deacetylation (Xiaoyi Li , Hui Li , Qian Liu , Ruitian Song , Huairen Zhang , Jie Pan , Zicong Li , Danhua Jiang) doi.org/10.1093/plce... #PlantScience @aspbofficial
FVE and HDA9 form a complex to promote thermomorphogenesis by reducing H2A.Z deposition through histone deacetylation
FVE-HDA9-mediated histone deacetylation limits H2A.Z deposition and, with INO80-dependent eviction, promotes H2A.Z depletion at PIF4-targeted auxin-related
doi.org
June 4, 2026 at 4:48 PM
chrstne.bsky.social
it will go well - i know it! and who doesn't like ino80/swr1?!
February 6, 2025 at 10:05 PM
tkozawa.bsky.social
Great work by Wang et al. (2025) on how #PlantTOR dynamically regulates the translation of a chromatin-associated complex for growth (CACG) in #Arabidopsis, which represses the expression of stress-responsive genes to coordinate plant growth and stress tolerance 🌱⚖️🏜️.
🔗 www.nature.com/articles/s41...
Fig. 6 | Working model for the role of the nutrient–TOR–CACG axis in balancing plant growth and stress tolerance. In the presence of adequate nutrients, TOR is active and promotes the translation of CACG mRNAs. The pyrimidine-rich motifs present in the 5′ UTRs of CACG mRNAs facilitate target selection by TOR. The CACG complex directly binds to numerous stress-responsive genes marked by histone acetylation, thereby repressing their transcription, which contributes to plant growth and development. Conversely, under nutrient-deficient conditions, TOR becomes inactive, leading to decreased translation of CACG mRNAs. This results in the alleviation of the repression of stress-response genes, causing reduced growth but increased stress tolerance. The nutrient–TOR–CACG axis is crucial for balancing plant growth with stress tolerance, promoting survival and reproductive success in variable environmental conditions. conditions. Image partially created in BioRender. He, X. Fig. 1 | Identification of a multi-subunit protein complex involved in nutrient-responsive growth. a) Protein–protein interaction networks were generated from mass spectrometry data using Cytoscape. The edges show the interactions identified by AP–MS. CACG subunits are shown in pink, while COMPASS subunits and the INO80 complex (INO80-C) are shown in blue. b) Interactions among CACG subunits as determined by Y2H and pull-down assays. Interactions revealed by Y2H assays are shown in yellow, those shown by pull-down assays in red and those confirmed by both methods in orange. Protein names in bold denote those analysed through pull-down assays. c) Detection of a high-molecular-weight complex by gel filtration. Proteins extracted from Flag-tagged GTE1, DP1, EMB1967 and CP2 transgenic plants were separated in a Superose 6 column (10/300 GL; GE Healthcare Life Sciences) and were detected by immunoblotting. d) Immunoblot analysis of protein levels in the indicated Flag-tagged transgenic plants under different nutrient conditions. Twelve-day-old plants grown in solid MS medium were transferred to liquid MS, H2O or reduced concentrations of MS for 4 days. The actin protein level is shown as a loading control. e) Fresh weight of wild-type plants irrigated with water or liquid MS medium (n = 24). The values are means ± s.d. f) Immunoblot analysis of protein levels in the indicated Flag-tagged transgenic plants irrigated with water or liquid MS medium. The actin protein level is shown as a loading control. g) Morphological phenotypes of 20-day-old wild-type and mutant plants (top) and 12-day-old wild-type and mutant plants (bottom). Scale bars, 1 cm. The experiments in c, d and f were repeated independently twice and showed similar results. Modified Fig. 4 | The CACG complex suppresses the expression of stress-responsive genes to coordinate plant growth and stress tolerance. g) Morphological phenotypes of wild-type plants and CACG mutants under control, drought stress and rehydration conditions. Twelve-day-old plants grown in solid MS medium were transferred to soil and subsequently subjected to drought treatment until the Col-0 plants began to show mortality. The phenotypes are shown before and three days after rehydration with MS. Plants watered with MS serve as the control. h) The survival rate of wild-type plants and CACG mutants under drought stress conditions (n = 24). Each bar shows the mean ± s.d. from three independent biological replicates. P values were determined using two-tailed Student’s t-tests. i) Immunoblot analysis of the protein levels of CACG components under the indicated conditions. TOR activity was monitored by the phosphorylation state of S6K. The actin protein level is shown as a loading control. The experiments were independently repeated twice and showed similar results. j) Statistical analysis of pS6K protein levels. Actin was used as a loading control. Each black dot represents an independent biological replicate.
September 26, 2025 at 8:26 PM
rbps-rna.bsky.social
Excited to share the publication of a co-first author paper in Epigenetics & Chromatin!

This collaborative side project with Dr. Jeff Fillingham’s lab at Toronto Metropolitan University explores the role of bromodomain proteins in regulating H2A.Z chromatin occupancy!

Read here:
rdcu.be/ez3eq
Bromodomain proteins IBD1 and IBD2 link histone acetylation to SWR1- and INO80-mediated H2A.Z regulation in Tetrahymena | Epigenetics & Chromatin
rdcu.be
August 9, 2025 at 9:59 PM
rridrobot.bsky.social
RRIDs were included in this in Biology of Reproduction paper. We value the author's support of reproducibility. #methodsmatter #STMpublishing #OpenScience
The Mouse INO80 Chromatin-Remodeling Complex Is an Essential Meiotic Factor for Spermatogenesis
Read the full paper: The Mouse INO80 Chromatin-Remodeling Complex Is an Essential Meiotic Factor for Spermatogenesis
doi.org
April 15, 2026 at 7:02 AM
rridrobot.bsky.social
The authors included RRIDs in their Glia paper! RRIDs improve reproducibility in scientific research. #methodsmatter #reproducibility #ReproducibleResearch
The INO80 Chromatin Remodeling Complex Regulates Histone H2A.Z Mobility and the G1‐S Transition in Oligodendrocyte Precursors
doi.org
May 7, 2025 at 11:02 PM
embojournal.org
A novel INO80 chromatin remodeler function in genome maintenance independent of its role in double-strand break repair:
Helle Ulrich and coworkers show it facilitates bypass of DNA lesions during replication by promoting repair of damage-induced daughter-strand gaps
www.embopress.org/doi/full/10....
October 15, 2025 at 1:29 PM
newphyt.bsky.social
Transcription elongation regulated by H2B deubiquitination and H2A.Z eviction enables fungal virulence

Li et al.

nph.onlinelibrary.wiley.com/doi/10.1111/...
Proposed model for the regulation of RNA polymerase II pause release mediated by FgUbp8 and the INO80-C in Fusarium graminearum.
November 25, 2025 at 1:02 PM
longtail-news.bsky.social
Feed: "Life Technology™ - Life Technology™ Science News"
By: Life Technology™ on Monday, November 24, 2025
Study Reveals DNA Flexibility Impact on INO80 Positioning
University of Texas MD Anderson Cancer Center study reveals DNA rigidity in nucleosome controls INO80 positioning, emphasizing DNA structure's role in transcription.
www.lifetechnology.com
November 25, 2025 at 2:33 AM