This State-of-the-Art Review synthesizes contemporary evidence on native joint septic arthritis epidemiology, microbiology, diagnosis, and management. We h

Astrocytes are associated with Alzheimer’s disease pathogenesis. We found that the transcription factor Sox9 functions to enhance astrocytic phagocytosis of Aβ plaques via MEGF10, and this clearance o...

Migraine is one of the most common neurological disorders, with a complex and incompletely understood pathogenesis. Recent studies have implicated the gut-brain axis in migraine pathophysiology. The gut microbiota and microbiota-related metabolites may influence migraine-relat...

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The Carnegie Mellon University Department of Biological Sciences is recruiting an tenure-track faculty member with an expected start date of Fall 2027

CONCLUSIONS: Animals are central to the ecology of SFTSV. Current evidence underscores the necessity of integrated, multisectoral One Health strategies for surveillance, risk communication, and control. Future research priorities include standardizing host-competence studies, harmonizing diagnostics, elucidating comparative immunopathology, and evaluating the feasibility and impact of intervention bundles, including potential veterinary vaccines.

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne zoonosis with a high case-fatality rate and expanding geographic range. Its persistence depends on complex cycles involv...

Filamentous phages play important roles in molecular biology and bacterial pathogenesis. There is therefore great interest in the genomes of the phages, including M13 and Pf, which infect Escherichia coli and Pseudomonas aeruginosa , respectively. However, efficient methods for purifying single-stranded DNA (ssDNA) from filamentous phages are scarce and rely mainly on repurposed commercial kits developed for dsDNA. These kits yield variable amounts of ssDNA, varying considerably from one phage to another, their solutions are proprietary and troubleshooting, and customizing the protocol to improve purification is not possible. Here, we developed and optimized two protocols based on ssDNA binding to silica columns in the presence of guanidine hydrochloride and potassium iodide chaotropic salts, for filamentous phages M13KO7 and Pf4. The protocols achieved 60 to 100% recovery, equal to or higher than the Monarch Spin gDNA Extraction and DNeasy Blood and Tissue kits, with similar A260/280 and A260/230 ratios, intact DNA in agarose gels, and no detectable host genomic DNA by PCR. Recovery depended critically on the chaotropic salts and on ethanol, and did not increase at lower pH, consistent with a water-exclusion model in which hydrophobic interactions and hydrogen bonding dominate over electrostatic repulsion. Phage-specific differences in recovery indicate that optimization may be needed for some phages, which the fully customizable nature of these protocols facilitates. Comparing the virion ssDNA with the double-stranded replicative form (RF) revealed genome variants in both phages, including a shorter M13KO7 form lacking the kanamycin resistance-p15A insert, and illustrates the value of analyzing both RF and ssDNA forms to study filamentous phage biology. Implementing these protocols in routine filamentous phage workflows should facilitate their characterization and engineering. ### Competing Interest Statement The authors have declared no competing interest. Stanford Maternal and Child Health Research Institute, Postdoctoral Fellowship Cystic Fibrosis Research Institute (CFRI), Elizabeth Nash Memorial Fellowship National Institutes of Health, K08 AI190313, R01 HL148184-01, R01 AI12492093, R01 DC019965, K24 AI166718, P01AI1960471 Cystic Fibrosis Foundation, https://ror.org/00ax59295