This is what happens when you remove the wall from plant cells. They become perfectly spherical and are called protoplasts.
These protoplasts were isolated from Arabidopsis and transformed with a vector to express cytosolic GFP (in orange). The chloroplast autofluorescence is in grey.
#microscopy
These protoplasts were isolated from Arabidopsis and transformed with a vector to express cytosolic GFP (in orange). The chloroplast autofluorescence is in grey.
#microscopy
September 22, 2025 at 7:36 PM
This is what happens when you remove the wall from plant cells. They become perfectly spherical and are called protoplasts.
These protoplasts were isolated from Arabidopsis and transformed with a vector to express cytosolic GFP (in orange). The chloroplast autofluorescence is in grey.
#microscopy
These protoplasts were isolated from Arabidopsis and transformed with a vector to express cytosolic GFP (in orange). The chloroplast autofluorescence is in grey.
#microscopy
March 17, 2026 at 8:23 AM
Can CRISPR edits enable precise tuning of plant gene expression? We think: yes.
In our newest manuscript, we measured the effects of >30,000 CRISPR-like promoter mutations in sorghum protoplasts.
In our newest manuscript, we measured the effects of >30,000 CRISPR-like promoter mutations in sorghum protoplasts.
March 31, 2026 at 4:31 PM
Can CRISPR edits enable precise tuning of plant gene expression? We think: yes.
In our newest manuscript, we measured the effects of >30,000 CRISPR-like promoter mutations in sorghum protoplasts.
In our newest manuscript, we measured the effects of >30,000 CRISPR-like promoter mutations in sorghum protoplasts.
Pleased to have finally got this method to work in wheat! Find out how you can identify direct targets of transcription factors in wheat using protoplasts and the TARGET method:
dx.doi.org/10.17504/pro...
A very useful method for our group to use! borrilllab.com
dx.doi.org/10.17504/pro...
A very useful method for our group to use! borrilllab.com
May 23, 2025 at 11:04 AM
Pleased to have finally got this method to work in wheat! Find out how you can identify direct targets of transcription factors in wheat using protoplasts and the TARGET method:
dx.doi.org/10.17504/pro...
A very useful method for our group to use! borrilllab.com
dx.doi.org/10.17504/pro...
A very useful method for our group to use! borrilllab.com
Moss (Physcomitrium patens) protoplasts (wallless cells) transformed with chloroplast envelope protein tagged with mGFP (green). The chlorophyll autofluorescence is in magenta.
The protrusions of the chloroplast envelope are called stromules.
#microscopymonday, #plantcells, #plantmicroscopy, #moss
The protrusions of the chloroplast envelope are called stromules.
#microscopymonday, #plantcells, #plantmicroscopy, #moss
March 9, 2026 at 3:06 PM
Moss (Physcomitrium patens) protoplasts (wallless cells) transformed with chloroplast envelope protein tagged with mGFP (green). The chlorophyll autofluorescence is in magenta.
The protrusions of the chloroplast envelope are called stromules.
#microscopymonday, #plantcells, #plantmicroscopy, #moss
The protrusions of the chloroplast envelope are called stromules.
#microscopymonday, #plantcells, #plantmicroscopy, #moss
Moving forward to a new era: just loaded our first single cell samples from a demo on the CellenONE using Arabidopsis protoplasts. Very curious to see the results! #proteomics #singlecellproteomics #plantscience #corefacility
July 10, 2025 at 12:52 PM
Moving forward to a new era: just loaded our first single cell samples from a demo on the CellenONE using Arabidopsis protoplasts. Very curious to see the results! #proteomics #singlecellproteomics #plantscience #corefacility
Some fluorescent Arabidopsis protoplasts for you all.
March 26, 2024 at 10:08 PM
Some fluorescent Arabidopsis protoplasts for you all.
October 27, 2023 at 1:10 PM
I wondered that as well. Usually these older protocols were root(callus)-derived protoplasts. I imagine that it's done to stress them less. For leaf protoplasts I never noticed an appreciable difference between dark/light
May 8, 2026 at 2:38 PM
I wondered that as well. Usually these older protocols were root(callus)-derived protoplasts. I imagine that it's done to stress them less. For leaf protoplasts I never noticed an appreciable difference between dark/light
I am very pleased to present the lab’s first #bluetorial!
This concerns our recent publication in Development @dev-journal.bsky.social doi.org/10.1242/dev.... This work, led by postdoc (and former grad student) Kelsey Reed, is the result of many years of hard work by a wonderful team. (1/n)
This concerns our recent publication in Development @dev-journal.bsky.social doi.org/10.1242/dev.... This work, led by postdoc (and former grad student) Kelsey Reed, is the result of many years of hard work by a wonderful team. (1/n)
A protoplast-based method to visualize early cell biological events in plant cellular reprogramming and regeneration
Summary: This study presents a live-imaging platform for tracking individual plant protoplasts in culture, enabling analysis of cellular processes, signaling dynamics and developmental transitions.
doi.org
September 25, 2026 at 2:23 PM
I am very pleased to present the lab’s first #bluetorial!
This concerns our recent publication in Development @dev-journal.bsky.social doi.org/10.1242/dev.... This work, led by postdoc (and former grad student) Kelsey Reed, is the result of many years of hard work by a wonderful team. (1/n)
This concerns our recent publication in Development @dev-journal.bsky.social doi.org/10.1242/dev.... This work, led by postdoc (and former grad student) Kelsey Reed, is the result of many years of hard work by a wonderful team. (1/n)
Because we call work in protoplasts in vivo. In planta tells you specifically the work was conducted in plants
March 19, 2026 at 3:37 PM
Because we call work in protoplasts in vivo. In planta tells you specifically the work was conducted in plants
We’re back at the @dsw-isp.bsky.social annual meeting this afternoon where @emilieknight.bsky.social is presenting a promising protoplast-based method to decipher gene networks in wheat. Protoplasts are living plant, bacterial, or fungal cells that have had their rigid outer cell walls removed.
February 26, 2026 at 3:04 PM
We’re back at the @dsw-isp.bsky.social annual meeting this afternoon where @emilieknight.bsky.social is presenting a promising protoplast-based method to decipher gene networks in wheat. Protoplasts are living plant, bacterial, or fungal cells that have had their rigid outer cell walls removed.
Looking for the direct target genes of a transcription factor in #wheat? Our protocol uses protoplasts, skipping transgenic plants to save months!
Great step by step protocol @emilieknight.bsky.social! Read more below ⬇️ Thanks to all contributors!
#plantscience @johninnescentre.bsky.social
Great step by step protocol @emilieknight.bsky.social! Read more below ⬇️ Thanks to all contributors!
#plantscience @johninnescentre.bsky.social
Pleased to have finally got this method to work in wheat! Find out how you can identify direct targets of transcription factors in wheat using protoplasts and the TARGET method:
dx.doi.org/10.17504/pro...
A very useful method for our group to use! borrilllab.com
dx.doi.org/10.17504/pro...
A very useful method for our group to use! borrilllab.com
May 23, 2025 at 11:31 AM
Looking for the direct target genes of a transcription factor in #wheat? Our protocol uses protoplasts, skipping transgenic plants to save months!
Great step by step protocol @emilieknight.bsky.social! Read more below ⬇️ Thanks to all contributors!
#plantscience @johninnescentre.bsky.social
Great step by step protocol @emilieknight.bsky.social! Read more below ⬇️ Thanks to all contributors!
#plantscience @johninnescentre.bsky.social
Thank you to @izzysaurlab.bsky.social, @teamthomma.bsky.social, and their teams for hosting our next two MadFungi workshops, "Expression in Barley Protoplasts" and "Expression of Antimicrobial Proteins," at
@unicologne.bsky.social. It was a great week with all of our MadFungi PhD candidates! 🍄
@unicologne.bsky.social. It was a great week with all of our MadFungi PhD candidates! 🍄
April 30, 2026 at 11:35 AM
Thank you to @izzysaurlab.bsky.social, @teamthomma.bsky.social, and their teams for hosting our next two MadFungi workshops, "Expression in Barley Protoplasts" and "Expression of Antimicrobial Proteins," at
@unicologne.bsky.social. It was a great week with all of our MadFungi PhD candidates! 🍄
@unicologne.bsky.social. It was a great week with all of our MadFungi PhD candidates! 🍄
Some of my postdoc work has been "exposed" today in @theplantjournal.bsky.social! 😁🎉🔬 We developed ExPOSE - a method to perform expansion microscopy in plant protoplast systems. Here's a quick🧵of the details: @meter76.bsky.social @lily-oconnor.bsky.social
onlinelibrary.wiley.com/doi/10.1111/...
onlinelibrary.wiley.com/doi/10.1111/...
ExPOSE: a comprehensive toolkit to perform expansion microscopy in plant protoplast systems
This method provides a robust approach for performing expansion microscopy in plant protoplasts for imaging single-cell and subcellular compartments at greatly enhanced resolution.
onlinelibrary.wiley.com
March 7, 2025 at 6:39 PM
Some of my postdoc work has been "exposed" today in @theplantjournal.bsky.social! 😁🎉🔬 We developed ExPOSE - a method to perform expansion microscopy in plant protoplast systems. Here's a quick🧵of the details: @meter76.bsky.social @lily-oconnor.bsky.social
onlinelibrary.wiley.com/doi/10.1111/...
onlinelibrary.wiley.com/doi/10.1111/...
The death of protoplasts. I was told that I made a restaurant for fungi by keeping my leaf protoplasts in not fully sterile conditions. However, they still look pretty.
#PlantScience
#Microscopy
#PlantScience
#Microscopy
July 9, 2024 at 10:20 AM
The death of protoplasts. I was told that I made a restaurant for fungi by keeping my leaf protoplasts in not fully sterile conditions. However, they still look pretty.
#PlantScience
#Microscopy
#PlantScience
#Microscopy
linet is a protoplast , colloquially referred to as an elf, but a human being all the same. i'm working on designs/lore for protoplasts and hope to post smth about them soon... linet himself has gone through a few design revisions and i'm still adjusting, along with the other charas designs
January 22, 2025 at 6:18 PM
linet is a protoplast , colloquially referred to as an elf, but a human being all the same. i'm working on designs/lore for protoplasts and hope to post smth about them soon... linet himself has gone through a few design revisions and i'm still adjusting, along with the other charas designs
Time-resolved tracking of cellulose biosynthesis and assembly during cell wall regeneration in live Arabidopsis protoplasts
#microscopy #plants #ScienceSky
www.science.org/doi/10.1126/...
#microscopy #plants #ScienceSky
www.science.org/doi/10.1126/...
Time-resolved tracking of cellulose biosynthesis and assembly during cell wall regeneration in live Arabidopsis protoplasts
Real-time imaging reveals multiscale dynamics of in vivo cellulose fibril network reconstruction in live plant protoplasts.
www.science.org
March 23, 2025 at 6:52 PM
Time-resolved tracking of cellulose biosynthesis and assembly during cell wall regeneration in live Arabidopsis protoplasts
#microscopy #plants #ScienceSky
www.science.org/doi/10.1126/...
#microscopy #plants #ScienceSky
www.science.org/doi/10.1126/...
Another nice thing about ExPOSE is its versatility. We used it to observe biomolecular condensates in Arabidopsis protoplasts and transcription factors in maize protoplasts at an enhanced resolution. 🧵
March 7, 2025 at 6:39 PM
Another nice thing about ExPOSE is its versatility. We used it to observe biomolecular condensates in Arabidopsis protoplasts and transcription factors in maize protoplasts at an enhanced resolution. 🧵
Unnati Sonawala talks about her work setting the stage for large-scale nematode effector screening in tomato roots.
Her project was a pilot in our new Postdoc Innovation Awards launched in 2024.
👉 tinyurl.com/ykc6ckee
@uprogress.bsky.social
@cropscicentre.bsky.social
#PlantScience 🧪
Her project was a pilot in our new Postdoc Innovation Awards launched in 2024.
👉 tinyurl.com/ykc6ckee
@uprogress.bsky.social
@cropscicentre.bsky.social
#PlantScience 🧪
May 8, 2025 at 11:33 AM
Unnati Sonawala talks about her work setting the stage for large-scale nematode effector screening in tomato roots.
Her project was a pilot in our new Postdoc Innovation Awards launched in 2024.
👉 tinyurl.com/ykc6ckee
@uprogress.bsky.social
@cropscicentre.bsky.social
#PlantScience 🧪
Her project was a pilot in our new Postdoc Innovation Awards launched in 2024.
👉 tinyurl.com/ykc6ckee
@uprogress.bsky.social
@cropscicentre.bsky.social
#PlantScience 🧪
♻️ Targeted genome modification in protoplasts of a tea cultivar Kolkhida using RNA-guided Cas9 endonuclease by Anastasiya Egorova and co-authors
Full #openaccess
👉 doi.org/10.1093/aobp...
#PlantScience
Full #openaccess
👉 doi.org/10.1093/aobp...
#PlantScience
Targeted genome modification in protoplasts of a tea cultivar Kolkhida using RNA-guided Cas9 endonuclease
The genome modification method for tea protoplasts was established. Three candidate genes were selected in the elite local tea cultivar Kolkhida. Construct
doi.org
February 4, 2026 at 7:03 AM
♻️ Targeted genome modification in protoplasts of a tea cultivar Kolkhida using RNA-guided Cas9 endonuclease by Anastasiya Egorova and co-authors
Full #openaccess
👉 doi.org/10.1093/aobp...
#PlantScience
Full #openaccess
👉 doi.org/10.1093/aobp...
#PlantScience
🧪🌾Are you familiar with protoplasts and why they are used as simplified models to investigate complex mechanisms such as how cells stick together?🔬
If not, take a look on this short popular science video created last year by Léa Bogdziewiez & Sonhita Chakraborty for the Fascination of #PlantDay👇:
If not, take a look on this short popular science video created last year by Léa Bogdziewiez & Sonhita Chakraborty for the Fascination of #PlantDay👇:
July 28, 2025 at 7:32 AM
🧪🌾Are you familiar with protoplasts and why they are used as simplified models to investigate complex mechanisms such as how cells stick together?🔬
If not, take a look on this short popular science video created last year by Léa Bogdziewiez & Sonhita Chakraborty for the Fascination of #PlantDay👇:
If not, take a look on this short popular science video created last year by Léa Bogdziewiez & Sonhita Chakraborty for the Fascination of #PlantDay👇:
"a recently developed single cell mass spectrometry method can be used to monitor the incorporation of an isotopically labeled precursor into single plant protoplasts." #PlantScience
From: Isotopic Labeling Analysis using Single Cell Mass Spectrometry
www.biorxiv.org/content/10.1...
From: Isotopic Labeling Analysis using Single Cell Mass Spectrometry
www.biorxiv.org/content/10.1...
Isotopic Labeling Analysis using Single Cell Mass Spectrometry
Here we show that a recently developed single cell mass spectrometry method can be used to monitor the incorporation of an isotopically labeled precursor into single plant protoplasts. Specifically, w...
www.biorxiv.org
April 29, 2025 at 8:54 PM
"a recently developed single cell mass spectrometry method can be used to monitor the incorporation of an isotopically labeled precursor into single plant protoplasts." #PlantScience
From: Isotopic Labeling Analysis using Single Cell Mass Spectrometry
www.biorxiv.org/content/10.1...
From: Isotopic Labeling Analysis using Single Cell Mass Spectrometry
www.biorxiv.org/content/10.1...
📜 Multiplexed effector screening for recognition by endogenous resistance genes using positive defense reporters in wheat protoplasts
🧑🔬 Salome Wilson, Bayantes Dagvadorj, @schwessinger.bsky.social, et al.
📔 @newphyt.bsky.social
🔗 nph.onlinelibrary.wiley.com/doi/10.1111/...
#️⃣ #PlantImmunity
🧑🔬 Salome Wilson, Bayantes Dagvadorj, @schwessinger.bsky.social, et al.
📔 @newphyt.bsky.social
🔗 nph.onlinelibrary.wiley.com/doi/10.1111/...
#️⃣ #PlantImmunity
nph.onlinelibrary.wiley.com
January 31, 2024 at 10:10 AM
📜 Multiplexed effector screening for recognition by endogenous resistance genes using positive defense reporters in wheat protoplasts
🧑🔬 Salome Wilson, Bayantes Dagvadorj, @schwessinger.bsky.social, et al.
📔 @newphyt.bsky.social
🔗 nph.onlinelibrary.wiley.com/doi/10.1111/...
#️⃣ #PlantImmunity
🧑🔬 Salome Wilson, Bayantes Dagvadorj, @schwessinger.bsky.social, et al.
📔 @newphyt.bsky.social
🔗 nph.onlinelibrary.wiley.com/doi/10.1111/...
#️⃣ #PlantImmunity
Great work by bachelor student Martijn Posthumus during his 10-week internship. He built tagged versions of GFP that are transported to different cellular compartments. We will use it to study protein sub-cellular localization in plants.
#plantbiology #protoplasts #GFP
#plantbiology #protoplasts #GFP
November 10, 2023 at 1:18 PM
Great work by bachelor student Martijn Posthumus during his 10-week internship. He built tagged versions of GFP that are transported to different cellular compartments. We will use it to study protein sub-cellular localization in plants.
#plantbiology #protoplasts #GFP
#plantbiology #protoplasts #GFP