Our new review in Current Opinion in Structural Biology is out!
We discuss how multicolour #SingleMolecule imaging reveals gene expression in action - from #Transcription and #RNA processing to #Translation, molecular coupling, and live-cell tracking.
doi.org/10.1016/j.sb...
#smFRET @embl.org
We discuss how multicolour #SingleMolecule imaging reveals gene expression in action - from #Transcription and #RNA processing to #Translation, molecular coupling, and live-cell tracking.
doi.org/10.1016/j.sb...
#smFRET @embl.org
September 29, 2026 at 9:08 AM
Our new review in Current Opinion in Structural Biology is out!
We discuss how multicolour #SingleMolecule imaging reveals gene expression in action - from #Transcription and #RNA processing to #Translation, molecular coupling, and live-cell tracking.
doi.org/10.1016/j.sb...
#smFRET @embl.org
We discuss how multicolour #SingleMolecule imaging reveals gene expression in action - from #Transcription and #RNA processing to #Translation, molecular coupling, and live-cell tracking.
doi.org/10.1016/j.sb...
#smFRET @embl.org
Check out our latest opinion on #smFRET to study #GPCR with Robert Quast @rbquast.bsky.social. We summarize recent findings, methodological advancements and talk about challenges and limitations to translate FRET into structural dynamics information
#openaccess
www.sciencedirect.com/science/arti...
#openaccess
www.sciencedirect.com/science/arti...
Recent advances on structure and dynamics of G protein–coupled receptors using single-molecule Förster resonance energy transfer
G protein–coupled receptors (GPCRs) mediate cellular responses to a wide array of extracellular stimuli, including photons, ions, small molecules, pep…
www.sciencedirect.com
August 31, 2026 at 11:06 AM
Check out our latest opinion on #smFRET to study #GPCR with Robert Quast @rbquast.bsky.social. We summarize recent findings, methodological advancements and talk about challenges and limitations to translate FRET into structural dynamics information
#openaccess
www.sciencedirect.com/science/arti...
#openaccess
www.sciencedirect.com/science/arti...
Interestingly, this is not a stable structure. SAXS and smFRET show that the existence of this pseudoknot is entirely dependent on the 2A protein - without it, the RNA stays as a stem-loop, unable to activate frameshifting.
5/8
5/8
August 24, 2026 at 11:52 AM
Interestingly, this is not a stable structure. SAXS and smFRET show that the existence of this pseudoknot is entirely dependent on the 2A protein - without it, the RNA stays as a stem-loop, unable to activate frameshifting.
5/8
5/8
#Biology isn’t static. 🧬 Discover how #smFRET, #FCS and #FCCS reveal in-solution dynamics - from conformational changes and drug mechanisms to ternary complex cooperativity and stability. See how dynamic measurements turn molecular snapshots into mechanistic insight > buff.ly/vvoGpwu
In-solution dynamics for mechanistic insights | Exciting Instruments
Discover how in-solution single-molecule dynamics add mechanistic insights across nutrient sensing, viral replication, and ternary complexes.
excitinginstruments.com
August 12, 2026 at 7:21 AM
#Biology isn’t static. 🧬 Discover how #smFRET, #FCS and #FCCS reveal in-solution dynamics - from conformational changes and drug mechanisms to ternary complex cooperativity and stability. See how dynamic measurements turn molecular snapshots into mechanistic insight > buff.ly/vvoGpwu
"PhotonFit has completely removed that friction." - Dr Janice Pata, Wadsworth Centre, NYSDOH. PhotonFit brings smFRET, FCS and FCCS into one intuitive workspace, so researchers can focus on biology instead of debugging code. Watch: www.youtube.com/watch?v=_8S7... #smFRET #FCS
August 6, 2026 at 9:12 AM
"PhotonFit has completely removed that friction." - Dr Janice Pata, Wadsworth Centre, NYSDOH. PhotonFit brings smFRET, FCS and FCCS into one intuitive workspace, so researchers can focus on biology instead of debugging code. Watch: www.youtube.com/watch?v=_8S7... #smFRET #FCS
Last month, I had the great joy of attending this year’s #BioPhySAL course in Spain. Grateful to the organizers and speakers Carlos Bustamante, @nogaleslab.bsky.social, @thevillalab.bsky.social, Taekjip Ha, Ibrahim Cissé, @zhuanglab.bsky.social, and more, who made this such a memorable experience.
July 24, 2026 at 7:18 PM
Last month, I had the great joy of attending this year’s #BioPhySAL course in Spain. Grateful to the organizers and speakers Carlos Bustamante, @nogaleslab.bsky.social, @thevillalab.bsky.social, Taekjip Ha, Ibrahim Cissé, @zhuanglab.bsky.social, and more, who made this such a memorable experience.
Curious about #smFRET data on #ssDNA? Our latest application note from the Antony Lab (Saint Louis University) shows how EI-FLEX data reveals that RPA wraps ssDNA rather than stretching it, resolving a long-standing question. Read more: buff.ly/N0hSA1h #DNA #AppNote
Protein-driven ssDNA conformational control | Exciting Instruments
Discover the linear relationship between FRET efficiency and ssDNA length, and how this can capture protein-mediated conformational control.
excitinginstruments.com
July 23, 2026 at 7:42 AM
Curious about #smFRET data on #ssDNA? Our latest application note from the Antony Lab (Saint Louis University) shows how EI-FLEX data reveals that RPA wraps ssDNA rather than stretching it, resolving a long-standing question. Read more: buff.ly/N0hSA1h #DNA #AppNote
Super work from @dozenoaks.bsky.social lab to help standardise #smFRET studies between in vitro and in cell models. 📏🔬🎇
A Universal Protein Ladder for Standardization of Diverse FRET Assays - Smith - Advanced Science - Wiley Online Library advanced.onlinelibrary.wiley.com/doi/10.1002/...
advanced.onlinelibrary.wiley.com
July 21, 2026 at 3:52 PM
Super work from @dozenoaks.bsky.social lab to help standardise #smFRET studies between in vitro and in cell models. 📏🔬🎇
FENNEC: photon-level deep learning for classifying bursts in diffusion-based single-molecule FRET [new]
...classifies smFRET bursts as dynamics, photobleaching, blinking from photon data, indep. of state count. Trained on sim. data.
...classifies smFRET bursts as dynamics, photobleaching, blinking from photon data, indep. of state count. Trained on sim. data.
July 17, 2026 at 2:16 AM
FENNEC: photon-level deep learning for classifying bursts in diffusion-based single-molecule FRET [new]
...classifies smFRET bursts as dynamics, photobleaching, blinking from photon data, indep. of state count. Trained on sim. data.
...classifies smFRET bursts as dynamics, photobleaching, blinking from photon data, indep. of state count. Trained on sim. data.
Benchmarking dynamic analysis methods in diffusion-based single-molecule FRET
Benchmarking dynamic analysis methods in diffusion-based single-molecule FRET
Crossley benchmarks widely used dynamic analysis methods for diffusion-based smFRET using simulated datasets, clarifying the timescales, burst number requirements, and FRET state separation each approach demands. The results offer practical guidelines for selecting analysis strategies and accurately interpreting conformational dynamics in biomolecules.
dlvr.it
July 14, 2026 at 6:49 PM
Benchmarking dynamic analysis methods in diffusion-based single-molecule FRET
📢 Today's the day! Join Tim Craggs and Prof. Taekjip Ha for this afternoon's Exciting Seminar celebrating 30 years of smFRET. Hear from the pioneer behind the first smFRET measurement and discover what's next. Register: us06web.zoom.us/meeting/regi... #biophysics #singlemoleculeFRET #virtualevent
July 13, 2026 at 8:04 AM
📢 Today's the day! Join Tim Craggs and Prof. Taekjip Ha for this afternoon's Exciting Seminar celebrating 30 years of smFRET. Hear from the pioneer behind the first smFRET measurement and discover what's next. Register: us06web.zoom.us/meeting/regi... #biophysics #singlemoleculeFRET #virtualevent
New Base by Base episode: we unpack a PNAS study showing Rpc34-WH2 shuttles among three positions in Pol III elongation complexes via smFRET and a novel thiol-capping SPAAC labeling s…
https://basebybase.com/episodes/base-by-base-413-rpc34-wh2-pol3-dynamics/
@pnas.org @pnasnexus.org @nasonline.org
https://basebybase.com/episodes/base-by-base-413-rpc34-wh2-pol3-dynamics/
@pnas.org @pnasnexus.org @nasonline.org
July 12, 2026 at 1:29 PM
New Base by Base episode: we unpack a PNAS study showing Rpc34-WH2 shuttles among three positions in Pol III elongation complexes via smFRET and a novel thiol-capping SPAAC labeling s…
https://basebybase.com/episodes/base-by-base-413-rpc34-wh2-pol3-dynamics/
@pnas.org @pnasnexus.org @nasonline.org
https://basebybase.com/episodes/base-by-base-413-rpc34-wh2-pol3-dynamics/
@pnas.org @pnasnexus.org @nasonline.org
Previous papers suggested that UL9 bends the ~70bp origin sequence to mediate strand separation. Using smFRET we indeed found some cases of origin bending but it was very rare (~3%).
July 3, 2026 at 5:18 AM
Previous papers suggested that UL9 bends the ~70bp origin sequence to mediate strand separation. Using smFRET we indeed found some cases of origin bending but it was very rare (~3%).
📢 Join us on 13 July for a virtual seminar on Conformational control: From single-molecule FRET to novel biotechnologies with our CEO Tim and Prof. Taekjip Ha.
11:00am EDT | 8:00am PDT | 4:00pm BST | 5:00pm CEST
Register: us06web.zoom.us/meeting/regi...
#smFRET #biophysics #singlemolecule
11:00am EDT | 8:00am PDT | 4:00pm BST | 5:00pm CEST
Register: us06web.zoom.us/meeting/regi...
#smFRET #biophysics #singlemolecule
July 1, 2026 at 7:18 AM
📢 Join us on 13 July for a virtual seminar on Conformational control: From single-molecule FRET to novel biotechnologies with our CEO Tim and Prof. Taekjip Ha.
11:00am EDT | 8:00am PDT | 4:00pm BST | 5:00pm CEST
Register: us06web.zoom.us/meeting/regi...
#smFRET #biophysics #singlemolecule
11:00am EDT | 8:00am PDT | 4:00pm BST | 5:00pm CEST
Register: us06web.zoom.us/meeting/regi...
#smFRET #biophysics #singlemolecule
🎉 30 years of single-molecule FRET! Since Taekjip Ha's landmark paper, smFRET has transformed molecular biology. Celebrate with us and share how smFRET has advanced your research: www.linkedin.com/pulse/30-yea... #smFRET
30 years of single-molecule FRET - a celebration 🎉
Hi there, Single-molecule FRET has been transforming how researchers investigate conformational changes, molecular interactions, and complex biological processes for the past 30 years. Today marks 30 ...
www.linkedin.com
June 25, 2026 at 6:04 AM
🎉 30 years of single-molecule FRET! Since Taekjip Ha's landmark paper, smFRET has transformed molecular biology. Celebrate with us and share how smFRET has advanced your research: www.linkedin.com/pulse/30-yea... #smFRET
Preprint alert! Using smFRET, we define the only essential ATP hydrolysis step in translation initiation. We show that eIF3 and eIF4B stimulate ATP-hydrolysis-dependent recycling of cap-stalled eIF4F, coupling eIF4F recycling to PIC loading onto an mRNA.🔬 doi.org/10.64898/202...
June 24, 2026 at 9:47 PM
Preprint alert! Using smFRET, we define the only essential ATP hydrolysis step in translation initiation. We show that eIF3 and eIF4B stimulate ATP-hydrolysis-dependent recycling of cap-stalled eIF4F, coupling eIF4F recycling to PIC loading onto an mRNA.🔬 doi.org/10.64898/202...
Beautiful example of how the EI-FLEX can be used to complement structural models of protein-RNA complexes with in-solution smFRET 🧬
🙌 Congrats to the Bonilla lab on their new preprint! Streit et al. combined cryo-EM, biochemistry, and smFRET on the EI-FLEX to uncover how flaviviral RNA dynamics guide assembly of the replication-initiation complex. A great example of why dynamics matter 💪 buff.ly/r3OHZrY
smFRET and cryo-EM reveal RNA dynamics | Exciting Instruments
See how smFRET on the EI-FLEX, combined with cryo-EM, revealed how dynamic RNA structures regulate RNA-protein interactions and drive flavivirus replication.
buff.ly
June 24, 2026 at 9:39 AM
Beautiful example of how the EI-FLEX can be used to complement structural models of protein-RNA complexes with in-solution smFRET 🧬
🙌 Congrats to the Bonilla lab on their new preprint! Streit et al. combined cryo-EM, biochemistry, and smFRET on the EI-FLEX to uncover how flaviviral RNA dynamics guide assembly of the replication-initiation complex. A great example of why dynamics matter 💪 buff.ly/r3OHZrY
smFRET and cryo-EM reveal RNA dynamics | Exciting Instruments
See how smFRET on the EI-FLEX, combined with cryo-EM, revealed how dynamic RNA structures regulate RNA-protein interactions and drive flavivirus replication.
buff.ly
June 24, 2026 at 9:27 AM
🙌 Congrats to the Bonilla lab on their new preprint! Streit et al. combined cryo-EM, biochemistry, and smFRET on the EI-FLEX to uncover how flaviviral RNA dynamics guide assembly of the replication-initiation complex. A great example of why dynamics matter 💪 buff.ly/r3OHZrY
3. The first use of alternating laser excitation (ALEX) for smFRET enabled single-molecule analysis of biomolecular structure and interactions simultaneously with smFRET – published by Kapanidis et al., in 2004 - www.pnas.org/doi/full/10....
June 23, 2026 at 9:33 AM
3. The first use of alternating laser excitation (ALEX) for smFRET enabled single-molecule analysis of biomolecular structure and interactions simultaneously with smFRET – published by Kapanidis et al., in 2004 - www.pnas.org/doi/full/10....
Join the next FRISBI webinar, in collab with FBI & ReNAFoBis, on June 25 at 13:00.
Robert Quast (CBS, Montpellier) will present his research combining genetic & multicolor smFRET microscopy to study the dynamics of GPCRs.
Register before June 24 - 15:00 ➡️ grist.numerique.gouv.fr/o/docs/forms...
Robert Quast (CBS, Montpellier) will present his research combining genetic & multicolor smFRET microscopy to study the dynamics of GPCRs.
Register before June 24 - 15:00 ➡️ grist.numerique.gouv.fr/o/docs/forms...
June 22, 2026 at 2:13 PM
Join the next FRISBI webinar, in collab with FBI & ReNAFoBis, on June 25 at 13:00.
Robert Quast (CBS, Montpellier) will present his research combining genetic & multicolor smFRET microscopy to study the dynamics of GPCRs.
Register before June 24 - 15:00 ➡️ grist.numerique.gouv.fr/o/docs/forms...
Robert Quast (CBS, Montpellier) will present his research combining genetic & multicolor smFRET microscopy to study the dynamics of GPCRs.
Register before June 24 - 15:00 ➡️ grist.numerique.gouv.fr/o/docs/forms...
5. The first use of smFRET in living cells to study conformational changes of SNARE proteins upon complex formation was performed in 2010 by Sakon et al. - www.nature.com/articles/nme...
June 22, 2026 at 2:03 PM
5. The first use of smFRET in living cells to study conformational changes of SNARE proteins upon complex formation was performed in 2010 by Sakon et al. - www.nature.com/articles/nme...
4. Also in 2004, Hohng et al. demonstrated the first use of three-colour smFRET using holiday junctions as a model system - extending smFRET's readout beyond a single 1D reaction coordinate - pmc.ncbi.nlm.nih.gov/articles/PMC...
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pmc.ncbi.nlm.nih.gov
June 22, 2026 at 2:02 PM
4. Also in 2004, Hohng et al. demonstrated the first use of three-colour smFRET using holiday junctions as a model system - extending smFRET's readout beyond a single 1D reaction coordinate - pmc.ncbi.nlm.nih.gov/articles/PMC...
2. Zhuang et al. published the first use of smFRET to track conformational changes of individual ribozymes in 2000, enabling the determination of rate constants and the characterisation of the transition state - pubmed.ncbi.nlm.nih.gov/10856219/
June 22, 2026 at 2:00 PM
2. Zhuang et al. published the first use of smFRET to track conformational changes of individual ribozymes in 2000, enabling the determination of rate constants and the characterisation of the transition state - pubmed.ncbi.nlm.nih.gov/10856219/
1. The first demonstration that smFRET can track the motion of a single molecule undergoing conformational changes was achieved by Ha et al. in 1999 - buff.ly/neFApLC
June 22, 2026 at 1:57 PM
1. The first demonstration that smFRET can track the motion of a single molecule undergoing conformational changes was achieved by Ha et al. in 1999 - buff.ly/neFApLC