#SingleMolecule
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quantum-si.bsky.social
One step closer on the path to 20. Another amino acid successfully detected on Proteus™. Read the PR here: bit.ly/4zl2IEn

#QSI #Proteus #SingleMolecule #ProteinSequencing #Proteomics
October 8, 2026 at 10:46 PM
olivierduss.bsky.social
Our new review in Current Opinion in Structural Biology is out!

We discuss how multicolour #SingleMolecule imaging reveals gene expression in action - from #Transcription and #RNA processing to #Translation, molecular coupling, and live-cell tracking.

doi.org/10.1016/j.sb...

#smFRET @embl.org
September 29, 2026 at 9:08 AM
quantum-si.bsky.social
Proteus™ data is coming. Join our lunchtime seminar today at #WorldHUPO to hear the most recent updates on Proteus from our CEO, Jeff Hawkins. Quantum-Si's seminar is today in Room 330. You won't want to miss this!

#QSI #ProteinSequencing #HUPO26 #Proteomics #Multiomics #SingleMolecule #Proteus
September 29, 2026 at 12:21 AM
natnano.nature.com
New paper:

Nanopore-enabled time-resolved monitoring of catecholamine-related phenylalanine metabolism.

"An engineered MspA #nanopore tracks #catecholamine biosynthesis at the #singlemolecule level, revealing pathway dynamics and enzymatic dysfunction."

www.nature.com/articles/s41...
Nanopore-enabled time-resolved monitoring of catecholamine-related phenylalanine metabolism - Nature Nanotechnology
An engineered bionanopore enables single-molecule tracking of catecholamine biosynthesis from phenylalanine to adrenaline, revealing time-resolved pathway dynamics and showing how impaired dehalogenas...
www.nature.com
September 1, 2026 at 9:52 AM
leidenphysics.bsky.social
👉 We are hiring!
#Postdoc project aimed at fabricating #graphene edge #sensors for #singlemolecule detection and biopolymer sequencing.
🔬&🧪Your challenge is to combine unique #chemistry and #physics techniques!
Apply before 15/9 edu.nl/pm8c9
September 1, 2026 at 10:04 AM
dulinlab.bsky.social
Follow ups are in preparations to further demonstrate the power of such high-throughput correlative MT-TIRF: stay tuned! #biophysics #microscopy #statistics #transcription #singlemolecule
August 17, 2026 at 7:22 PM
biophysicalsoc.bsky.social
Stop by @madcitylabs1, Booth #501 to learn about their piezo #nanopositioners, #singlemolecule microscopes, and atomic force microscopes. New! Motorized micromirrors for RM21 microscope, MadView quad image splitter for microscopy. #bps2025 #biophysics
February 16, 2025 at 9:30 PM
grnfluoresceblg.bsky.social
"The mechanism of mRNA activation"
A preprint looking at the role of eIF4F in translation initiation at single molecule resolution.
#mRNA #translation #ribosome #singlemolecule 🧪🔬

www.biorxiv.org/content/10.1...
The mechanism of mRNA activation
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
www.biorxiv.org
November 16, 2023 at 5:54 AM
ibfg.bsky.social
👉 Don’t miss this opportunity to discover how single-molecule techniques uncover the mechanics of protein machines in DNA metabolism 🔬🧬⚙️

#SingleMolecule #DNAMetabolism #MolecularMachines #GenomeStability
April 14, 2026 at 9:10 AM
jitumayorlab.bsky.social
Postdoc alert! Into single molecule microscopy? Join us at the CMCB to develop single molecule approaches for studying membrane organisation, receptor dynamics and signalling in live cells. Apply by 2/8/26
jitumayorlab.wixsite.com/ourlab/post/...

#SingleMolecule #MembraneBiophysics #PostdocJobs
jitumayorlab.wixsite.com
July 17, 2026 at 1:32 PM
lisannes.bsky.social
First post 🥳!

Excited to share that Jacob Lewis and I were awarded an ARC Discovery Project Grant to study how mitochondrial DNA is copied using #cryoEM and #singlemolecule fluorescence microscopy🔬. Postdoc & PhD opportunities announced soon!
November 28, 2024 at 11:26 PM
biosofttau.bsky.social
#BioSoft Seminar
Jan 7 | 11:00| Kaplun #118
Dr. Ivan Terterov (WIZ)
Correlation functions for stochastic processes with biased observations
Single-molecule FRET, photon detection & recovering true dynamics from biased data. Students & researchers welcome!
#TAU #Biophysics #SingleMolecule #FRET
January 6, 2026 at 4:46 PM
alirezamashaghi.bsky.social
PhD student Laurens Heling from my laboratory will be presenting a the #LACDR Spring Symposium!

"Single Molecule Analysis Reveals Allosteric Regulation of #Androgen #Receptor DNA Binding​"

#SingleMolecule #Biophysics #Endocrinology
May 11, 2025 at 8:42 AM
alirezamashaghi.bsky.social
We recently reported the first single molecule protein folding analysis using optical tweezers inside cytosol! This allows for including the full complexity of cellular chaperone network & beyond.

#SingleMolecule #Biophysics #Protein #Chaperone #OpticalTweezers #ChemSky

phys.org/news/2023-08...
Biological origami at molecular level: Cytosolic interactome protects against protein unfolding
Human cells are protecting their proteins from unfolding and aggregating. That's what biophysicist Alireza Mashaghi and his team discovered after seven years of in-depth research into the folding mech...
phys.org
November 24, 2024 at 8:54 AM
olivierduss.bsky.social
Using #SingleMolecule imaging we find that TFs:

-boost co-transcriptional #ribosomal #RNA processing!

-their binding #dynamics fundamentally differ for mRNA (transient) versus rRNA #transcription (stable)!

@biorxivpreprint.bsky.social Anastasiia Chaban @embl.org

www.biorxiv.org/content/10.6...
March 29, 2026 at 2:38 PM
schwalbe-lab.bsky.social
Take a look at the publication of our former lab member Nina Kubatova in @pnas.org 😇
"Dimerization propensity of the β1-adrenergic receptor in lipid nanodiscs probed by DEER and single-molecule spectroscopies"
Full text: www.pnas.org/doi/10.1073/...
#DEER #protein #SingleMolecule #GPCR
Dimerization propensity of the β1-adrenergic receptor in lipid nanodiscs probed by DEER and single-molecule spectroscopies | PNAS
G protein–coupled receptors (GPCRs) comprise a large class of membrane proteins that mediate cellular responses to a wide range of external signals...
www.pnas.org
September 25, 2025 at 8:27 AM
hagenom.bsky.social
Fiery kickoff for the 30th Single Molecule Workshop!
I’m super excited to see so many great scientists and big names in the field gathered here in Berlin.
Let’s go #WS30 🚀

#WS30 #SingleMolecule #Spectroscopy #Microscopy
September 23, 2025 at 7:29 AM
madcitylabs.bsky.social
Stop by Booth 508 at #BPS2026 and chat with us about the newfeatures of the 3rd generation RM21 MicroMirror TIRF Microscope. High signal-to-noise ratio, adaptability for a variety of single molecule techniques, streamlined alignment process. #singlemolecule #biophysics
February 22, 2026 at 8:45 PM
madcitylabs.bsky.social
Heading to Methods and Applications in Fluorescence (MAF2025) in Montreal next week. Stop by and chat with us about the new 3rd generation RM21 MicroMirror TIRF Microscope. High signal to noise ratio, adaptability for single molecule techniques, streamlined alignment. #singlemolecule
August 18, 2025 at 2:31 PM
madcitylabs.bsky.social
#BPS2025 Exhibit Hall opens today! Stop by Booth 501 to check out our #nanopositioners, microscope stages & decks, AFM, and new improvements to RM21 MicroMirror TIRF microscope. Hall opens at 10:00am. #singlemolecule #biophysics @biophysicalsoc.bsky.social
February 16, 2025 at 7:16 PM
madcitylabs.bsky.social
We are excited to be part of #bps2025. Looking forward to discovering new #biophysics research. Curious about our positioning and microscopy products? Stop by Booth 501, Sunday through Tuesday, for a chat. #nanopositioning #singlemolecule #forcemicroscopy #biophysics @biophysicalsociety
February 11, 2025 at 4:00 PM
schaefferlab.bsky.social
How do plant kinesins work at the single-molecule level?

We break it all down in our new review "Plant kinesins: a bottom-up approach - from single molecules to function" published in JXB @jxbotany.bsky.social 🎉

Check it out: doi.org/10.1093/jxb/...

#Plant #Kinesin #SingleMolecule #ZMBP
Plant kinesins: a bottom-up approach – from single molecules to function
Abstract. During land colonisation, plants evolved new microtubule structures that have no functional analogues in opisthokonts, namely animals and fungi.
doi.org
July 14, 2026 at 9:28 AM
schaefferlab.bsky.social
New paper alert! 📄
We’re thrilled to share a new publication from our lab:

"Monomers and short oligomers of human RAD52 promote single-strand annealing"

Check it out: www.pnas.org/doi/10.1073/...

#DNARepair #RAD52 #MolecularBiology #SingleMolecule #PhDResearch #MassPhotometry #StructuralBiology
Monomers and short oligomers of human RAD52 promote single-strand annealing | PNAS
Genome maintenance and stability rely on the repair of DNA double-strand breaks. Breaks can be repaired via the single-strand-annealing pathway med...
www.pnas.org
April 8, 2025 at 8:11 AM
schaefferlab.bsky.social
Great time at #SMBioDresden — old friends, new friends, and exciting science!

#SingleMolecule #ScienceCommunity #AcademicLife #Dresden #MPI-CBG
March 19, 2025 at 7:46 AM
chromatinhaiku.bsky.social
Transcribing Pol2
With elongation factors
Kinetic control

#ChromatinHaiku #SingleMolecule
www.nature.com/articles/s41...
Fig. 1: Real-time observation of mammalian transcription elongation. a, Composite structural model of the human transcription EC with a set of elongation factors, as supplied in our system, decorating the Pol II surface. The model incorporates published structures for the mammalian EC (PDB 6TED), TFIIS (PDB 8A40), ELOF1 (PDB 8B3F) and a fitted AlphaFold prediction of IWS1 based on the position of Spn1 in the yeast EC (PDB 7XN7). b, Schematic of the single-molecule experimental setup. The starting location of the EC is marked by the ATTO647N-labeled RNA, while Cy3-labeled DNA probes that hybridize to the nascent RNA indicate active elongation. c, An example of transcription elongation by a single mammalian EC observed in real time. Top: kymograph showing the EC position on the DNA template, indicated by RNA (magenta) and probe (green) signals, as a function of time. Bottom: elongation trajectory extracted from the kymograph. Raw data (gray dots) were fitted to discrete linear segments (black line). Change points are marked as filled circles. The slopes for each segment are indicated, differentiating active elongation (blue) from pausing or stalling events (red). d, Eight additional examples of fitted elongation trajectories by individual mammalian ECs aligned by their starting positions. NTP, nucleoside triphosphate. Fig. 7: Summary of the distinct roles that each elongation factor serves in mammalian Pol II transcription on DNA. The relative EC activity for each factor omission condition is color coded as indicated on the right. Clockwise from the top left: ΔTFIIS increases pausing but has a minimal effect on the elongation speed; ΔP-TEFb abrogates the phosphorylation of Pol II CTD and DSIF, which in turn destabilizes the binding of PAF1C, SPT6 and RTF1, thereby severely reducing the elongation speed; ΔPAF1C exerts the most direct negative impact on EC speed and also completely abolishes RTF1 recruitment; ΔRTF1 shifts the EC from high speed to medium speed; ΔSPT6 reduces EC speed by destabilizing PAF1C binding even though SPT6 itself does not directly activate EC; ΔDSIF exerts a dual effect on EC kinetics by reducing its pausing and also lowering its speed partially via the destabilization of RTF1 binding.
November 27, 2025 at 12:57 PM