SMLM image of Endoplasmic Reticulum imaged with Abbelight SAFe MN360.
#SuperResolution #SMLM #FluorescenceFriday
#SuperResolution #SMLM #FluorescenceFriday
December 6, 2024 at 7:56 PM
SMLM image of Endoplasmic Reticulum imaged with Abbelight SAFe MN360.
#SuperResolution #SMLM #FluorescenceFriday
#SuperResolution #SMLM #FluorescenceFriday
Make your own PDMS+micromirror device for single-objective light-sheet SMLM: www.biorxiv.org/content/10.6...
April 13, 2026 at 2:00 PM
Make your own PDMS+micromirror device for single-objective light-sheet SMLM: www.biorxiv.org/content/10.6...
Some early attempts to show 3D SMLM data in #bigvolumebrowser :
4M points from @shuangfu-shawn.bsky.social re-analysis of our LLS-paint data (see recent retweet).
It can be overlaid with any volumetric data input.
@christlet.bsky.social are you still interested in Fiji SMLM renderer?
4M points from @shuangfu-shawn.bsky.social re-analysis of our LLS-paint data (see recent retweet).
It can be overlaid with any volumetric data input.
@christlet.bsky.social are you still interested in Fiji SMLM renderer?
May 12, 2025 at 10:41 PM
Some early attempts to show 3D SMLM data in #bigvolumebrowser :
4M points from @shuangfu-shawn.bsky.social re-analysis of our LLS-paint data (see recent retweet).
It can be overlaid with any volumetric data input.
@christlet.bsky.social are you still interested in Fiji SMLM renderer?
4M points from @shuangfu-shawn.bsky.social re-analysis of our LLS-paint data (see recent retweet).
It can be overlaid with any volumetric data input.
@christlet.bsky.social are you still interested in Fiji SMLM renderer?
semalem si r0h uruh arah bilang bakalan ada vl09 di bukperta…apakah akan ada sesi nyempil tipis” dua bocah itu👀🤣 mana yg nntn smlm kebanyakan esqeemo wkwkwk
February 2, 2026 at 8:20 AM
semalem si r0h uruh arah bilang bakalan ada vl09 di bukperta…apakah akan ada sesi nyempil tipis” dua bocah itu👀🤣 mana yg nntn smlm kebanyakan esqeemo wkwkwk
Comparing SMLM and volumetric (max proj) data in #bigvolumebrowser
June 13, 2025 at 3:19 PM
Comparing SMLM and volumetric (max proj) data in #bigvolumebrowser
@loicaroyer.bsky.social and pity there is no SMLM data in the showreel. This microscopy branch was splatting Gaussians before splatting became popular.:)
September 23, 2026 at 12:16 PM
@loicaroyer.bsky.social and pity there is no SMLM data in the showreel. This microscopy branch was splatting Gaussians before splatting became popular.:)
Controlled blinking of upconverting nanoparticles - no bleaching means attractive potential as an SMLM probe! www.nature.com/articles/s41...
July 27, 2026 at 12:18 PM
Controlled blinking of upconverting nanoparticles - no bleaching means attractive potential as an SMLM probe! www.nature.com/articles/s41...
🔬 Happy to share our new paper showing that fluorophore brightness open a new dimension in SMLM microscopy !
Thanks to all authors @laurent-le.bsky.social S. K. Sreenivas @emmanuelfort.bsky.social !
@ismolab.bsky.social @instlangevin.bsky.social @cnrs.fr
rdcu.be/fmIeD
doi.org/10.1038/s415...
Thanks to all authors @laurent-le.bsky.social S. K. Sreenivas @emmanuelfort.bsky.social !
@ismolab.bsky.social @instlangevin.bsky.social @cnrs.fr
rdcu.be/fmIeD
doi.org/10.1038/s415...
June 5, 2026 at 10:20 AM
🔬 Happy to share our new paper showing that fluorophore brightness open a new dimension in SMLM microscopy !
Thanks to all authors @laurent-le.bsky.social S. K. Sreenivas @emmanuelfort.bsky.social !
@ismolab.bsky.social @instlangevin.bsky.social @cnrs.fr
rdcu.be/fmIeD
doi.org/10.1038/s415...
Thanks to all authors @laurent-le.bsky.social S. K. Sreenivas @emmanuelfort.bsky.social !
@ismolab.bsky.social @instlangevin.bsky.social @cnrs.fr
rdcu.be/fmIeD
doi.org/10.1038/s415...
Big news for correlative cryogenic SMLM/ET! With my colleague Soheil, we (Mahamid and @jonasries.bsky.social labs) indicate lamella-specific illumination can allow 500-1500x higher laser intensities, opening massive new possibilities for correlative imaging #teamtomo
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
April 22, 2026 at 3:43 PM
Big news for correlative cryogenic SMLM/ET! With my colleague Soheil, we (Mahamid and @jonasries.bsky.social labs) indicate lamella-specific illumination can allow 500-1500x higher laser intensities, opening massive new possibilities for correlative imaging #teamtomo
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
We are kicking off #SMLMS2025 and are happy to welcome you all in Bonn! @smlm-symposium.bsky.social www.smlms.org
August 27, 2025 at 11:06 AM
We are kicking off #SMLMS2025 and are happy to welcome you all in Bonn! @smlm-symposium.bsky.social www.smlms.org
We introduce mirror-enhanced 4Pi-SMLM (me4Pi-SMLM), a streamlined single-objective configuration that leverages mirror-based retroreflection of the illumination beam to achieve near-isotropic localization precision of 2–3 nm, enabling unambiguous resolution of diverse ultrastructural features.
Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging - @yongdengzhang.bsky.social go.nature.com/3Ocpn3N
Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging - Nature Biotechnology
Single-molecule 4Pi microscopy is simplified and made accessible by using a single objective.
go.nature.com
March 31, 2026 at 7:29 PM
We introduce mirror-enhanced 4Pi-SMLM (me4Pi-SMLM), a streamlined single-objective configuration that leverages mirror-based retroreflection of the illumination beam to achieve near-isotropic localization precision of 2–3 nm, enabling unambiguous resolution of diverse ultrastructural features.
Fireworks = SMLM?!?🤓
August 17, 2026 at 10:26 PM
Fireworks = SMLM?!?🤓
We present a versatile microfluidics platform that makes multiplexed super-resolution imaging more accessible, reproducible, and high-throughput.
👉 BioRxiv: www.biorxiv.org/content/10.1...
#SuperResolution #DNApaint #Microfluidics #SMLM #OpenScience #multiplexing
👉 BioRxiv: www.biorxiv.org/content/10.1...
#SuperResolution #DNApaint #Microfluidics #SMLM #OpenScience #multiplexing
October 15, 2025 at 12:29 PM
We present a versatile microfluidics platform that makes multiplexed super-resolution imaging more accessible, reproducible, and high-throughput.
👉 BioRxiv: www.biorxiv.org/content/10.1...
#SuperResolution #DNApaint #Microfluidics #SMLM #OpenScience #multiplexing
👉 BioRxiv: www.biorxiv.org/content/10.1...
#SuperResolution #DNApaint #Microfluidics #SMLM #OpenScience #multiplexing
Some good SMLM preprints and papers out in the last couple of days! First is @wmarkbates.bsky.social's COMET drift correction approach 1/5
Delighted to share our preprint describing COMET, a new approach to drift correction for #SMLM, which performs exceptionally well in practice. Check out the benchmarking results compared to other methods! :) Thanks to @lreinkensmeier.bsky.social for a superb effort.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
April 1, 2026 at 9:25 AM
Some good SMLM preprints and papers out in the last couple of days! First is @wmarkbates.bsky.social's COMET drift correction approach 1/5
Delighted to share our preprint describing COMET, a new approach to drift correction for #SMLM, which performs exceptionally well in practice. Check out the benchmarking results compared to other methods! :) Thanks to @lreinkensmeier.bsky.social for a superb effort.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
March 31, 2026 at 3:50 PM
Delighted to share our preprint describing COMET, a new approach to drift correction for #SMLM, which performs exceptionally well in practice. Check out the benchmarking results compared to other methods! :) Thanks to @lreinkensmeier.bsky.social for a superb effort.
www.biorxiv.org/cgi/content/...
www.biorxiv.org/cgi/content/...
Anybody remember SMLM (Smell em) cards we were given in Germany back when?. LTC Barry McCaffery promised $50 and a case of beer to whomever got photos of those guys following our convoys.
January 25, 2026 at 10:45 PM
Anybody remember SMLM (Smell em) cards we were given in Germany back when?. LTC Barry McCaffery promised $50 and a case of beer to whomever got photos of those guys following our convoys.
MULTIFANDOM
englot, lingorm, fayeyoko, faymay, smlm, milklove/ciize, bminenear, anunkamol, atommer...
englot, lingorm, fayeyoko, faymay, smlm, milklove/ciize, bminenear, anunkamol, atommer...
August 31, 2024 at 4:23 AM
MULTIFANDOM
englot, lingorm, fayeyoko, faymay, smlm, milklove/ciize, bminenear, anunkamol, atommer...
englot, lingorm, fayeyoko, faymay, smlm, milklove/ciize, bminenear, anunkamol, atommer...
@christlet.bsky.social kicking off day 2 of #cbias2024 - SMLM for neuron structural studies.
November 26, 2024 at 9:31 AM
@christlet.bsky.social kicking off day 2 of #cbias2024 - SMLM for neuron structural studies.
🔬Happy to share our new preprint, where brightness is used to identify single molecule emission in 2D/3D, with a single camera ! Part of the PhD work of @laurent-le.bsky.social and Surabhi, great collaboration with @emmanuelfort.bsky.social from @instlangevin.bsky.social @ismolab.bsky.social #SMLM
March 4, 2025 at 8:58 AM
🔬Happy to share our new preprint, where brightness is used to identify single molecule emission in 2D/3D, with a single camera ! Part of the PhD work of @laurent-le.bsky.social and Surabhi, great collaboration with @emmanuelfort.bsky.social from @instlangevin.bsky.social @ismolab.bsky.social #SMLM
Huge thanks to J.Bewersdorf @fbzt.bsky.social A.Sentenac @vicidominilab.bsky.social for the super-resolution session #MiFobio2025 organized with @lydiadanglot.bsky.social @gdrimabio.bsky.social. Great overview of SMLM+3D/multitarget imaging, MinFlux, structured illumination towards RIM and ISM
October 12, 2025 at 9:14 AM
Huge thanks to J.Bewersdorf @fbzt.bsky.social A.Sentenac @vicidominilab.bsky.social for the super-resolution session #MiFobio2025 organized with @lydiadanglot.bsky.social @gdrimabio.bsky.social. Great overview of SMLM+3D/multitarget imaging, MinFlux, structured illumination towards RIM and ISM
I missed this beautiful work showing that myelin compaction depends on actin disassembly. It has SMLM, correlative AFM with a clever fluorescent nanoscale reporter of compaction, in vivo cell-specific actin disassembly using deActs… check it out! www.biorxiv.org/content/10.6...
August 10, 2026 at 8:47 PM
I missed this beautiful work showing that myelin compaction depends on actin disassembly. It has SMLM, correlative AFM with a clever fluorescent nanoscale reporter of compaction, in vivo cell-specific actin disassembly using deActs… check it out! www.biorxiv.org/content/10.6...
🔬Check out this clever approach for 3D SMLM by Yoav Shechtman @technion.bsky.social and @gustavssonlab.bsky.social ... stunning images! 👉🏻 doi.org/10.64898/202...
August 1, 2026 at 9:48 AM
🔬Check out this clever approach for 3D SMLM by Yoav Shechtman @technion.bsky.social and @gustavssonlab.bsky.social ... stunning images! 👉🏻 doi.org/10.64898/202...
(1/n) A simple, ultrastable, and cost-effective oxygen-scavenging system for long-term DNA-PAINT imaging
Please check out our new study on the benefits of sodium sulfite as oxygen scavenging system for DNA-PAINT.
👇🧵
#SuperResolution #DNAPAINT #SMLM #Microscopy
Please check out our new study on the benefits of sodium sulfite as oxygen scavenging system for DNA-PAINT.
👇🧵
#SuperResolution #DNAPAINT #SMLM #Microscopy
July 21, 2025 at 3:46 PM
(1/n) A simple, ultrastable, and cost-effective oxygen-scavenging system for long-term DNA-PAINT imaging
Please check out our new study on the benefits of sodium sulfite as oxygen scavenging system for DNA-PAINT.
👇🧵
#SuperResolution #DNAPAINT #SMLM #Microscopy
Please check out our new study on the benefits of sodium sulfite as oxygen scavenging system for DNA-PAINT.
👇🧵
#SuperResolution #DNAPAINT #SMLM #Microscopy
Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging - @yongdengzhang.bsky.social go.nature.com/3Ocpn3N
Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging - Nature Biotechnology
Single-molecule 4Pi microscopy is simplified and made accessible by using a single objective.
go.nature.com
March 31, 2026 at 2:12 PM
Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging - @yongdengzhang.bsky.social go.nature.com/3Ocpn3N
Resolution improvement and multicolor demixing with event-based SMLM #EveSMLM by Manon Albecq and Clément Cabriel @clementcabriel.bsky.social
#Mifobio2025
@gdrimabio.bsky.social
#Mifobio2025
@gdrimabio.bsky.social
October 15, 2025 at 12:06 PM
Resolution improvement and multicolor demixing with event-based SMLM #EveSMLM by Manon Albecq and Clément Cabriel @clementcabriel.bsky.social
#Mifobio2025
@gdrimabio.bsky.social
#Mifobio2025
@gdrimabio.bsky.social