Excited to share our new preprint on co-transcriptional splicing! U1 snRNP is recruited to RNA polymerase II through multiple interaction sites, with elongation factor SPT6 binding directly to the U1 snRNP protein.
www.biorxiv.org/content/10.1...
#cryoEM #transcription #splicing
www.biorxiv.org/content/10.1...
#cryoEM #transcription #splicing
March 24, 2025 at 3:56 PM
Excited to share our new preprint on co-transcriptional splicing! U1 snRNP is recruited to RNA polymerase II through multiple interaction sites, with elongation factor SPT6 binding directly to the U1 snRNP protein.
www.biorxiv.org/content/10.1...
#cryoEM #transcription #splicing
www.biorxiv.org/content/10.1...
#cryoEM #transcription #splicing
Very excited to share our new paper out now in
@MolecularCell. We show how U1 snRNP -a well-known splicing factor- regulates the activity of alternative promoters in human cells www.cell.com/molecular-ce...
@MolecularCell. We show how U1 snRNP -a well-known splicing factor- regulates the activity of alternative promoters in human cells www.cell.com/molecular-ce...
U1 snRNP regulates alternative promoter activity by inhibiting premature polyadenylation
Kim et al. uncover a role for U1 snRNP in regulating internal promoter activity. Beyond
its canonical role in splicing, U1 snRNP suppresses premature polyadenylation, enabling
upstream transcription t...
www.cell.com
May 16, 2025 at 3:35 PM
Very excited to share our new paper out now in
@MolecularCell. We show how U1 snRNP -a well-known splicing factor- regulates the activity of alternative promoters in human cells www.cell.com/molecular-ce...
@MolecularCell. We show how U1 snRNP -a well-known splicing factor- regulates the activity of alternative promoters in human cells www.cell.com/molecular-ce...
Excited to share our latest work! We found transcription factor SPT6 and phosphorylated Pol II CTD help recruit U1 snRNP to elongating Pol II, allowing efficient co-transcriptional splicing.
Glad to be featured in the Editors’ Highlights!
www.nature.com/articles/s41...
#splicing #cryoEM
Glad to be featured in the Editors’ Highlights!
www.nature.com/articles/s41...
#splicing #cryoEM
July 10, 2025 at 2:29 PM
Excited to share our latest work! We found transcription factor SPT6 and phosphorylated Pol II CTD help recruit U1 snRNP to elongating Pol II, allowing efficient co-transcriptional splicing.
Glad to be featured in the Editors’ Highlights!
www.nature.com/articles/s41...
#splicing #cryoEM
Glad to be featured in the Editors’ Highlights!
www.nature.com/articles/s41...
#splicing #cryoEM
A deserved celebration after the hard work 🍾🥂. @waelskamel.bsky.social and collaborators from the @castello-lab.bsky.social discovered that the U2 snRNP displays a moonlighting antiviral role and uses the U2 snRNA to interact with viral RNA 🤯🤯🤯 t.co/DJlXFxmGEv
December 17, 2024 at 7:04 PM
A deserved celebration after the hard work 🍾🥂. @waelskamel.bsky.social and collaborators from the @castello-lab.bsky.social discovered that the U2 snRNP displays a moonlighting antiviral role and uses the U2 snRNA to interact with viral RNA 🤯🤯🤯 t.co/DJlXFxmGEv
Excited to share some new work from our group - www.biorxiv.org/content/10.1...
A great collaboration with @kurianlab.bsky.social that began with how an RBP (QKI) controls cardiomyocyte function, and led to uncovering a unique mechanism of direct interaction with U6 & the tri-snRNP at weak 5'SS
A great collaboration with @kurianlab.bsky.social that began with how an RBP (QKI) controls cardiomyocyte function, and led to uncovering a unique mechanism of direct interaction with U6 & the tri-snRNP at weak 5'SS
QKI ensures splicing fidelity during cardiogenesis by engaging the U6 tri-snRNP to activate splicing at weak 5ʹ splice sites
During organogenesis, precise pre-mRNA splicing is essential to assemble tissue architecture. Many developmentally essential exons bear weak 5'splice sites (5'SS) yet are spliced with high precision, ...
www.biorxiv.org
September 8, 2025 at 4:43 PM
Excited to share some new work from our group - www.biorxiv.org/content/10.1...
A great collaboration with @kurianlab.bsky.social that began with how an RBP (QKI) controls cardiomyocyte function, and led to uncovering a unique mechanism of direct interaction with U6 & the tri-snRNP at weak 5'SS
A great collaboration with @kurianlab.bsky.social that began with how an RBP (QKI) controls cardiomyocyte function, and led to uncovering a unique mechanism of direct interaction with U6 & the tri-snRNP at weak 5'SS
U1 snRNP regulates alternative promoter activity by inhibiting premature polyadenylation
U1 snRNP regulates alternative promoter activity by inhibiting premature polyadenylation
Kim et al. uncover a role for U1 snRNP in regulating internal promoter activity. Beyond its canonical role in splicing, U1 snRNP suppresses premature polyadenylation, enabling upstream transcription to activate internal promoters via nucleosome destabilization, RNA polymerase II pause release, and promoter escape. Targeted U1 snRNP reveals a mechanism to control alternative isoform expression.
dlvr.it
May 18, 2025 at 3:20 PM
U1 snRNP regulates alternative promoter activity by inhibiting premature polyadenylation
See also @wojtekgalej.bsky.social and
tinyurl.com/3sfthabb
#MinorSpliceosome #RNAsplicing #snRNP #U12Introns
tinyurl.com/3sfthabb
#MinorSpliceosome #RNAsplicing #snRNP #U12Introns
October 1, 2025 at 8:27 AM
#Efeméride La bioquímica Joan Elaine Argetsinger Steitz (1941) cumple hoy años.
Es conocida por sus descubrimientos sobre el ARN.
Es conocida por sus descubrimientos sobre el ARN.
Joan A. Steitz, bioquímica - Mujeres con ciencia
Joan Elaine Argetsinger Steitz. Bioquímica. ARN. ARN mensajero. Ribosoma. Ribonucleoproteínas. SnRNP. Células eucariotas. Intrones.
s.mtrbio.com
January 26, 2025 at 9:20 AM
#Efeméride La bioquímica Joan Elaine Argetsinger Steitz (1941) cumple hoy años.
Es conocida por sus descubrimientos sobre el ARN.
Es conocida por sus descubrimientos sobre el ARN.
This is a cool study by group @novartis.bsky.social using photoaffinity/click chemistry to map RNA binding sites of splicing modulator drugs
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Photoaffinity enabled transcriptome-wide identification of splice modulating small molecule-RNA binding events in native cells
Splice modulating small molecules have been developed to promote the U1 snRNP to engage with pre-mRNAs with strong and altered sequence preference. Transcriptomic profiling of bulk RNA from compound t...
www.biorxiv.org
November 15, 2024 at 2:43 PM
This is a cool study by group @novartis.bsky.social using photoaffinity/click chemistry to map RNA binding sites of splicing modulator drugs
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
New from Hope Merens in @stirlingchurchman.bsky.social lab!
SRSF1 regulates polyadenylation site selection independently of and through coordination with U1 snRNP
Hope E. Merens et al.
www.biorxiv.org/content/10.6...
SRSF1 regulates polyadenylation site selection independently of and through coordination with U1 snRNP
Hope E. Merens et al.
www.biorxiv.org/content/10.6...
www.biorxiv.org
April 6, 2026 at 7:48 PM
New from Hope Merens in @stirlingchurchman.bsky.social lab!
SRSF1 regulates polyadenylation site selection independently of and through coordination with U1 snRNP
Hope E. Merens et al.
www.biorxiv.org/content/10.6...
SRSF1 regulates polyadenylation site selection independently of and through coordination with U1 snRNP
Hope E. Merens et al.
www.biorxiv.org/content/10.6...
Many conserved exons in the heart and brain utilize weak 5 ′ splice sites, yet they are accurately spliced. But how? We show that splicing fidelity is actively enforced through a QKI-U6 checkpoint at the U1→U6 handover in essential cardiac genes during organogenesis.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
QKI ensures splicing fidelity during cardiogenesis by engaging the U6 tri-snRNP to activate splicing at weak 5ʹ splice sites
During organogenesis, precise pre-mRNA splicing is essential to assemble tissue architecture. Many developmentally essential exons bear weak 5'splice sites (5'SS) yet are spliced with high precision, ...
www.biorxiv.org
September 8, 2025 at 6:12 AM
Many conserved exons in the heart and brain utilize weak 5 ′ splice sites, yet they are accurately spliced. But how? We show that splicing fidelity is actively enforced through a QKI-U6 checkpoint at the U1→U6 handover in essential cardiac genes during organogenesis.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
More people should pay attention to snRNP recycling!♻️
July 10, 2025 at 2:06 PM
More people should pay attention to snRNP recycling!♻️
The LMB warmly welcomes Reinhard Lührmann to deliver the Max Perutz Lecture at 11am (BST) on Monday 13 July titled ‘Structural insights into the cascade of snRNP remodelling steps leading to the formation of a catalytically activated spliceosome’
Read more here: mrclmb.ac.uk/news-events/...
#LMBNews
Read more here: mrclmb.ac.uk/news-events/...
#LMBNews
July 6, 2026 at 11:38 AM
The LMB warmly welcomes Reinhard Lührmann to deliver the Max Perutz Lecture at 11am (BST) on Monday 13 July titled ‘Structural insights into the cascade of snRNP remodelling steps leading to the formation of a catalytically activated spliceosome’
Read more here: mrclmb.ac.uk/news-events/...
#LMBNews
Read more here: mrclmb.ac.uk/news-events/...
#LMBNews
New OA Article: "The Arabidopsis U1 snRNP regulates mRNA 3′-end processing" rdcu.be/dU3nb
A plant U1 snRNP complex associates with 200 proteins and conducts splicing-independent roles by safeguarding RNAs against premature cleavage and polyadenylation (telescripting). #PlantScience
A plant U1 snRNP complex associates with 200 proteins and conducts splicing-independent roles by safeguarding RNAs against premature cleavage and polyadenylation (telescripting). #PlantScience
September 25, 2024 at 10:23 AM
New OA Article: "The Arabidopsis U1 snRNP regulates mRNA 3′-end processing" rdcu.be/dU3nb
A plant U1 snRNP complex associates with 200 proteins and conducts splicing-independent roles by safeguarding RNAs against premature cleavage and polyadenylation (telescripting). #PlantScience
A plant U1 snRNP complex associates with 200 proteins and conducts splicing-independent roles by safeguarding RNAs against premature cleavage and polyadenylation (telescripting). #PlantScience
SF3B3 / SF3B5 form a metazoan specific transcription module of the U2 snRNP that coordinates Pol II elongation in a splicing independent manner
SF3B3 / SF3B5 form a metazoan specific transcription module of the U2 snRNP that coordinates Pol II elongation in a splicing independent manner
Co-transcriptional splicing is a conserved feature of eukaryotic gene expression. However, establishing the functional nature of this process has been difficult. Here using high throughput CRISPR/Cas9...
doi.org
July 22, 2026 at 2:31 PM
SF3B3 / SF3B5 form a metazoan specific transcription module of the U2 snRNP that coordinates Pol II elongation in a splicing independent manner
Joan Elaine Steitz (26. Januar 1941).
Sie ist eine US-amerikanische Biochemikerin und Professorin an der Yale University..
Steitz leistete Pionierarbeit auf dem Gebiet der RNA-Forschung. Sie entdeckte die snRNP-Komplexe.
Sie ist eine US-amerikanische Biochemikerin und Professorin an der Yale University..
Steitz leistete Pionierarbeit auf dem Gebiet der RNA-Forschung. Sie entdeckte die snRNP-Komplexe.
January 26, 2026 at 7:26 AM
Joan Elaine Steitz (26. Januar 1941).
Sie ist eine US-amerikanische Biochemikerin und Professorin an der Yale University..
Steitz leistete Pionierarbeit auf dem Gebiet der RNA-Forschung. Sie entdeckte die snRNP-Komplexe.
Sie ist eine US-amerikanische Biochemikerin und Professorin an der Yale University..
Steitz leistete Pionierarbeit auf dem Gebiet der RNA-Forschung. Sie entdeckte die snRNP-Komplexe.
First up: Mathieu Quinodoz, variants in RNU4-2 and 4 U6 genes cause dominant retinitis pigmentosa - 1.4% of undiagnosed.
Likely a different mechanism than NDD - delayed tri-snRNP formation.
Preprint: www.medrxiv.org/content/10.1...
@carlorivolta.bsky.social @susanneroosing.bsky.social #eshg2025
Likely a different mechanism than NDD - delayed tri-snRNP formation.
Preprint: www.medrxiv.org/content/10.1...
@carlorivolta.bsky.social @susanneroosing.bsky.social #eshg2025
www.medrxiv.org
May 25, 2025 at 8:45 AM
First up: Mathieu Quinodoz, variants in RNU4-2 and 4 U6 genes cause dominant retinitis pigmentosa - 1.4% of undiagnosed.
Likely a different mechanism than NDD - delayed tri-snRNP formation.
Preprint: www.medrxiv.org/content/10.1...
@carlorivolta.bsky.social @susanneroosing.bsky.social #eshg2025
Likely a different mechanism than NDD - delayed tri-snRNP formation.
Preprint: www.medrxiv.org/content/10.1...
@carlorivolta.bsky.social @susanneroosing.bsky.social #eshg2025
Great news for @uam-ibmib.bsky.social research groups: OPUS @ncn.gov.pl grant was awarded to Artur Jarmołowski to study U1 snRNP & Sonata to Halina Petrykowska to investigate Male-specific microRNAs - big congratulations!!! #NCNtoTlen #3ProcentNaNaukę
May 29, 2025 at 7:55 AM
Great news for @uam-ibmib.bsky.social research groups: OPUS @ncn.gov.pl grant was awarded to Artur Jarmołowski to study U1 snRNP & Sonata to Halina Petrykowska to investigate Male-specific microRNAs - big congratulations!!! #NCNtoTlen #3ProcentNaNaukę
snRNP, pronounced "snerp" en.wikipedia.org/wiki/snRNP
snRNP - Wikipedia
en.wikipedia.org
June 25, 2026 at 3:09 AM
snRNP, pronounced "snerp" en.wikipedia.org/wiki/snRNP
#Efeméride La bioquímica Joan Elaine Argetsinger Steitz (1941) cumple hoy años.
Es conocida por sus descubrimientos sobre el ARN.
Es conocida por sus descubrimientos sobre el ARN.
Joan A. Steitz, bioquímica - Mujeres con ciencia
Joan Elaine Argetsinger Steitz. Bioquímica. ARN. ARN mensajero. Ribosoma. Ribonucleoproteínas. SnRNP. Células eucariotas. Intrones.
mujeresconciencia.com
January 26, 2026 at 9:20 AM
#Efeméride La bioquímica Joan Elaine Argetsinger Steitz (1941) cumple hoy años.
Es conocida por sus descubrimientos sobre el ARN.
Es conocida por sus descubrimientos sobre el ARN.
U1 snRNP-Specific U1C Acts as the Gatekeeper of the Survival of Motor Neurons (SMN) Complex in snRNP Biogenesis https://www.biorxiv.org/content/10.1101/2024.11.11.623077v1
U1 snRNP-Specific U1C Acts as the Gatekeeper of the Survival of Motor Neurons (SMN) Complex in snRNP Biogenesis https://www.biorxiv.org/content/10.1101/2024.11.11.623077v1
The stability and abundance of spliceosomal snRNPs are determined by the assembly of an Sm protein r
www.biorxiv.org
November 12, 2024 at 2:45 AM
U1 snRNP-Specific U1C Acts as the Gatekeeper of the Survival of Motor Neurons (SMN) Complex in snRNP Biogenesis https://www.biorxiv.org/content/10.1101/2024.11.11.623077v1