The authors of this paper reversed the amino acid sequence of a nanobody and apparently expected it to function like the forward sequence.

Yeah, no, that's not how proteins work.

#Science @Strucbio #structuralbiology

https://pubpeer.com/publications/BB01ACDF81B4854C50ADF3C6B4DBBC

The name of my specialism is Macromolecular Crystallography.
I refine models against crystallographic data that I have collected.

Therefore I am in Macro Data Refinement I shall take no further questions.

@strucbio #structuralbiology

How we peer review structural data | Nature Structural & Molecular Biology

Of interest to @strucbio #structuralbiology

https://www.nature.com/articles/s41594-025-01567-9

The huge protein database that spawned AlphaFold and biology’s AI revolution
@strucbio
#structuralbiology #AlphaFold #nobelprize

https://www.nature.com/articles/d41586-024-03423-0

#science #biosky #structbio #strucbio (for those of you acrosss the pond) #hps

@ccpem.bsky.social
#cryoEM #cryoET #teamTOMO #structure #strucbio
I'd call this version 0.0.0. it's highly functional. Coded by parallel Claude agents and tested by running #Relion tutorials. This WebUI wraps #IsoNet2 into a Relion interface, internalizes picking jobs and ships securely in browser

Structural mechanism of CB1R binding to peripheral and biased inverse agonists | Nature Communications

Lovely bit of work. #cryoem #crystallography #Science #research @strucbio

https://www.nature.com/articles/s41467-024-54206-0

Do a PhD with us!

Receptor tyrosine kinases! Brains! Structures! Psychedelics(maybe).

#phd #cryoem #crystallography #Science #research @strucbio […]

An example I use in class is the plant JA receptor. It took 20+ years of genetics from a mutant to the gene likely being the JA receptor and other mutants to show it was JA-ILE not JA. The capstone paper was structural bio validating intxn with 3D model. So language went from genetics to strucbio.

Ccp4 is back online ! @strucbio
Collaborative Computational Project No. 4 – Software for Macromolecular X-Ray Crystallography
https://www.ccp4.ac.uk/

#CCP4 seems to be down ! 🫤 Does anyone know something about it ?
@strucbio

<a href="https://bsky.app/profile/did:plc:ip6kjvs3qmhirc52tlza4qs4" class="hover:underline text-blue-600 dark:text-sky-400 no-card-link" target="_blank" rel="noopener" data-link="bsky-mention">@xtaldave @strucbio I keep thinking I need to hit you up and ask about material property references because I imagine you're forgotten more chemistry than I ever learned. But then I remember crystallography implies solids and I'm faced more with liquids and gases so I might be barking up the […]

Amazing paper on how #hiv import its capsid into the nucleus of human cells !
DOI:10.1016/j.cell.2024.12.008
@strucbio
https://www.biophys.mpg.de/2842024/hivcapsid-cracks-the-npc?c=2011019

@strucbio @xtaldave [Checks calendar] [relaxes]

No no no no.

"Predicted". Not "determined". Not "solved" It is a hypothetical model. Not a "structure".

Get it right.

#nobelprize #structuralbiology @strucbio #AlphaFold #RoseTTAFold

@xtaldave @strucbio

Everybody with heart on the left will be eliminated.

After years of procrastinating I finally got around to publishing my Python module for reading mmCIF files.
Check it out: https://pypi.org/project/mmcifbuddy/ and feel free to let me know how it goes!
#crystallography #structuralbiology #mmcif
cc: @strucbio @xtaldave