Edismauro Freitas-Filho, Ph.D.
@efreitasfilho.bsky.social
Postdoc at the Department of Cell and Molecular Biology, USP/Brazil. Exploring how vesicular traffic regulates immune cell function.
Pinned
RAB5c coordinates early endosome-to-phagosome trafficking to drive LC3-associated phagocytosis in macrophages — and without it, fungal killing fails even when ROS production goes up.
New paper out in #ScienceAdvances. Thread below 🧵
New paper out in #ScienceAdvances. Thread below 🧵
How does the V-ATPase transmembrane core reach the phagosome during LAP (LC3 associated phagocytosis)?
Live imaging shows V0 transfer occurs via contact between V0+ organelles and the phagosome membrane, a step controlled by RAB5c+ early endosomes.
*STELLARIS Confocal Microscope #LeicaMicrosystems
Live imaging shows V0 transfer occurs via contact between V0+ organelles and the phagosome membrane, a step controlled by RAB5c+ early endosomes.
*STELLARIS Confocal Microscope #LeicaMicrosystems
May 20, 2026 at 7:00 AM
How does the V-ATPase transmembrane core reach the phagosome during LAP (LC3 associated phagocytosis)?
Live imaging shows V0 transfer occurs via contact between V0+ organelles and the phagosome membrane, a step controlled by RAB5c+ early endosomes.
*STELLARIS Confocal Microscope #LeicaMicrosystems
Live imaging shows V0 transfer occurs via contact between V0+ organelles and the phagosome membrane, a step controlled by RAB5c+ early endosomes.
*STELLARIS Confocal Microscope #LeicaMicrosystems
A follow-up on one of the more unexpected findings from our recent @ScienceAdvances paper: RAB5c-dependent trafficking of the V-ATPase V0 sector to phagosomes. 🧵
May 19, 2026 at 12:16 PM
A follow-up on one of the more unexpected findings from our recent @ScienceAdvances paper: RAB5c-dependent trafficking of the V-ATPase V0 sector to phagosomes. 🧵
Reposted by Edismauro Freitas-Filho, Ph.D.
There’s been lots of great science published in the past two weeks on #autophagy and #mTOR topics. Anyone with an interest in this area should look at these 👇🏼👇🏼👇🏼
biomed.news/bims-auttor/...
biomed.news/bims-auttor/...
biomed.news/bims-auttor/...
biomed.news/bims-auttor/...
Blue Power Ranger Billy: Okay, Now That's Cool!
ALT: Blue Power Ranger Billy: Okay, Now That's Cool!
static.klipy.com
May 17, 2026 at 12:06 PM
There’s been lots of great science published in the past two weeks on #autophagy and #mTOR topics. Anyone with an interest in this area should look at these 👇🏼👇🏼👇🏼
biomed.news/bims-auttor/...
biomed.news/bims-auttor/...
biomed.news/bims-auttor/...
biomed.news/bims-auttor/...
RAB5c coordinates early endosome-to-phagosome trafficking to drive LC3-associated phagocytosis in macrophages — and without it, fungal killing fails even when ROS production goes up.
New paper out in #ScienceAdvances. Thread below 🧵
New paper out in #ScienceAdvances. Thread below 🧵
May 12, 2026 at 6:45 AM
RAB5c coordinates early endosome-to-phagosome trafficking to drive LC3-associated phagocytosis in macrophages — and without it, fungal killing fails even when ROS production goes up.
New paper out in #ScienceAdvances. Thread below 🧵
New paper out in #ScienceAdvances. Thread below 🧵
Reposted by Edismauro Freitas-Filho, Ph.D.
Autophagy-lysosome pathways are known targets of intracellular bacteria – Ivan Đikić @idikic.bsky.social et al find Legionella uses phosphoribosyl-ubiquitination via SidE to alter autophagic SNAREs & create a bacteria-containing vacuole unable to fuse with lysosomes
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
June 12, 2025 at 11:17 AM
Autophagy-lysosome pathways are known targets of intracellular bacteria – Ivan Đikić @idikic.bsky.social et al find Legionella uses phosphoribosyl-ubiquitination via SidE to alter autophagic SNAREs & create a bacteria-containing vacuole unable to fuse with lysosomes
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
Reposted by Edismauro Freitas-Filho, Ph.D.
Phosphoribosyl ubiquitination of SNARE proteins regulate autophagy in Legionella infection https://www.biorxiv.org/content/10.1101/2025.05.19.654886v1
May 20, 2025 at 12:30 AM
Phosphoribosyl ubiquitination of SNARE proteins regulate autophagy in Legionella infection https://www.biorxiv.org/content/10.1101/2025.05.19.654886v1
Reposted by Edismauro Freitas-Filho, Ph.D.
Reposted by Edismauro Freitas-Filho, Ph.D.
#Autophagy and #mTOR papers and preprints from last week. As always a great selection, enjoy! 👌
biomed.news/bims-auttor/...
biomed.news/bims-auttor/...
a man in a suit and tie is sitting at a table with his eyes closed and a pile of food ..
ALT: a man in a suit and tie is sitting at a table with his eyes closed and a pile of food ..
media.tenor.com
April 13, 2025 at 11:49 AM
#Autophagy and #mTOR papers and preprints from last week. As always a great selection, enjoy! 👌
biomed.news/bims-auttor/...
biomed.news/bims-auttor/...
Reposted by Edismauro Freitas-Filho, Ph.D.
Excited to share our latest paper in Science Advances! We finally dissected a new pathway for mitochondrial quality control upon mtDNA replication stress! Let’s start a short thread! 🧵
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Retromer promotes the lysosomal turnover of mtDNA
Lysosomal uptake of mtDNA controls mitochondrial quality.
www.science.org
April 4, 2025 at 6:42 PM
Excited to share our latest paper in Science Advances! We finally dissected a new pathway for mitochondrial quality control upon mtDNA replication stress! Let’s start a short thread! 🧵
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Edismauro Freitas-Filho, Ph.D.
#Autophagy and #mTOR papers from last week, lots of relevant stuff as always, enjoy!
biomed.news/bims-auttor/...
biomed.news/bims-auttor/...
a man in a suit and tie is asking if he read it
ALT: a man in a suit and tie is asking if he read it
media.tenor.com
March 30, 2025 at 12:35 PM
#Autophagy and #mTOR papers from last week, lots of relevant stuff as always, enjoy!
biomed.news/bims-auttor/...
biomed.news/bims-auttor/...
Reposted by Edismauro Freitas-Filho, Ph.D.
RAB5c controls the assembly of non-canonical autophagy machinery to promote phagosome maturation and microbicidal function of macrophages https://www.biorxiv.org/content/10.1101/2025.03.25.645097v1
March 29, 2025 at 5:15 AM
RAB5c controls the assembly of non-canonical autophagy machinery to promote phagosome maturation and microbicidal function of macrophages https://www.biorxiv.org/content/10.1101/2025.03.25.645097v1
Reposted by Edismauro Freitas-Filho, Ph.D.
Happy to share the latest work from the lab @crick.ac.uk. Outstanding work from Di Chen and Tony Fearns showing the link between Mtb membrane damage, calcium leakage and ATG8/LC3 lipidation in macrophages. Congratulations Di and Tony!
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Mycobacterium tuberculosis phagosome Ca2+ leakage triggers multimembrane ATG8/LC3 lipidation to restrict damage in human macrophages
Ca2+ leakage triggers LC3 lipidation on multimembrane as Mtb phagosome damage response, independent of autophagy.
www.science.org
March 27, 2025 at 7:13 AM
Happy to share the latest work from the lab @crick.ac.uk. Outstanding work from Di Chen and Tony Fearns showing the link between Mtb membrane damage, calcium leakage and ATG8/LC3 lipidation in macrophages. Congratulations Di and Tony!
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Edismauro Freitas-Filho, Ph.D.
Exciting news from the lab out today in Science - the structure of human PINK1 on a TOM-VDAC complex!
@wehi-research.bsky.social
@wehi-research.bsky.social
Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/...
@wehi-research.bsky.social @komanderlab.bsky.social
@wehi-research.bsky.social @komanderlab.bsky.social
March 13, 2025 at 7:32 PM
Exciting news from the lab out today in Science - the structure of human PINK1 on a TOM-VDAC complex!
@wehi-research.bsky.social
@wehi-research.bsky.social
🚀Our latest work reveals how RAB5c orchestrates LC3-associated phagocytosis (LAP) to boost macrophage defense against A. fumigatus! 🦠🔬
🧵 Dive into the mechanisms linking endosomal traffic to innate immunity!
#Immunology #Autophagy #Phagocytosis #Macrophage #Preprint
doi.org/10.1101/2025...
🧵 Dive into the mechanisms linking endosomal traffic to innate immunity!
#Immunology #Autophagy #Phagocytosis #Macrophage #Preprint
doi.org/10.1101/2025...
March 29, 2025 at 7:28 PM
🚀Our latest work reveals how RAB5c orchestrates LC3-associated phagocytosis (LAP) to boost macrophage defense against A. fumigatus! 🦠🔬
🧵 Dive into the mechanisms linking endosomal traffic to innate immunity!
#Immunology #Autophagy #Phagocytosis #Macrophage #Preprint
doi.org/10.1101/2025...
🧵 Dive into the mechanisms linking endosomal traffic to innate immunity!
#Immunology #Autophagy #Phagocytosis #Macrophage #Preprint
doi.org/10.1101/2025...