Rosy Favicchio
@rosyf.bsky.social
💙 bluesky research 💙 reproducible science is excellent science. Editor @NatureProtocols / I have views and they’re mine!
Currently based in China
Currently based in China
Pinned
Rosy Favicchio
@rosyf.bsky.social
· Feb 27
A protocol for SIMworks, an integrated software suite for quality-controlled, augmented quantitative structured illumination microscopy (SIM).
@natprot.nature.com @fenaochs.bsky.social
🧪 🔬 #imaging
www.nature.com/articles/s41...
@natprot.nature.com @fenaochs.bsky.social
🧪 🔬 #imaging
www.nature.com/articles/s41...
SIMworks—an integrated software suite for quality-controlled augmented quantitative structured illumination microscopy - Nature Protocols
SIMworks is an integrated software suite for quality-controlled augmented quantitative structured illumination microscopy (SIM), enabling both new learners and experienced users to implement best prac...
www.nature.com
September 25, 2026 at 9:15 AM
A protocol for SIMworks, an integrated software suite for quality-controlled, augmented quantitative structured illumination microscopy (SIM).
@natprot.nature.com @fenaochs.bsky.social
🧪 🔬 #imaging
www.nature.com/articles/s41...
@natprot.nature.com @fenaochs.bsky.social
🧪 🔬 #imaging
www.nature.com/articles/s41...
Tissue expansion mass spectrometry imaging enables high-spatial-resolution mapping of lipids, metabolites, proteins/peptides and N-glycans, providing comprehensive molecular multiomics. @natprot.nature.com
🧪 🔬 #imaging #omics
🧪 🔬 #imaging #omics
Tissue expansion mass spectrometry imaging (TEMI) for high-spatial-resolution multiomics molecular mapping - Nature Protocols
Tissue expansion mass spectrometry imaging enables the mapping of lipids, metabolites, proteins, peptides and N-glycans at high spatial resolution.
www.nature.com
September 25, 2026 at 9:08 AM
Tissue expansion mass spectrometry imaging enables high-spatial-resolution mapping of lipids, metabolites, proteins/peptides and N-glycans, providing comprehensive molecular multiomics. @natprot.nature.com
🧪 🔬 #imaging #omics
🧪 🔬 #imaging #omics
A Protocol for Trace-n-Seq: a method that combines retrograde tracing with fluorescence-activated cell sorting and single-cell sequencing to molecularly profile individual neurons innervating healthy or diseased tissues. @natprot.nature.com
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Trace-n-Seq combines retrograde fluorescent tracing with sorting and single-cell sequencing of innervating peripheral neurons - Nature Protocols
This Protocol uses retrograde axonal tracing and fluorescence-activated cell sorting to transcriptionally profile innervating neurons at single-cell resolution.
www.nature.com
September 25, 2026 at 8:57 AM
A Protocol for Trace-n-Seq: a method that combines retrograde tracing with fluorescence-activated cell sorting and single-cell sequencing to molecularly profile individual neurons innervating healthy or diseased tissues. @natprot.nature.com
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Reposted by Rosy Favicchio
Good luck to all colleagues going into the new university year, persuading students that learning to think isn't the same thing as getting the right answer.
September 17, 2026 at 12:36 PM
Good luck to all colleagues going into the new university year, persuading students that learning to think isn't the same thing as getting the right answer.
This collection is on proteomics!
@natprot.nature.com
A collection of proteomics protocols from Nature Protocols focussing on our most recent content.
🧪 #proteomics #MassSpec
@natprot.nature.com
A collection of proteomics protocols from Nature Protocols focussing on our most recent content.
🧪 #proteomics #MassSpec
Proteomics protocols
A collection of proteomics protocols from Nature Protocols focussing on our most recent content.
www.nature.com
September 17, 2026 at 12:46 PM
This collection is on proteomics!
@natprot.nature.com
A collection of proteomics protocols from Nature Protocols focussing on our most recent content.
🧪 #proteomics #MassSpec
@natprot.nature.com
A collection of proteomics protocols from Nature Protocols focussing on our most recent content.
🧪 #proteomics #MassSpec
Oh we have been busy! Here another collection:
@natprot.nature.com
The protocols cover key stages of metabolite research, including sample collection, metabolite extraction, chromatographic separation, mass spectrometric analysis and data processing.
🧪 #metabolomics #chemsky
@natprot.nature.com
The protocols cover key stages of metabolite research, including sample collection, metabolite extraction, chromatographic separation, mass spectrometric analysis and data processing.
🧪 #metabolomics #chemsky
Metabolomics and metabolite analysis protocols
This Collection from Nature Protocols covers metabolomics and metabolite analysis. The protocols cover key stages of metabolite research, including sample ...
www.nature.com
September 17, 2026 at 12:43 PM
Oh we have been busy! Here another collection:
@natprot.nature.com
The protocols cover key stages of metabolite research, including sample collection, metabolite extraction, chromatographic separation, mass spectrometric analysis and data processing.
🧪 #metabolomics #chemsky
@natprot.nature.com
The protocols cover key stages of metabolite research, including sample collection, metabolite extraction, chromatographic separation, mass spectrometric analysis and data processing.
🧪 #metabolomics #chemsky
In this collection we highlight protocols for synthesizing, processing and characterizing advanced materials, with particular emphasis on 2D materials and nanoscale materials.
@natprot.nature.com
🧪 #2Dmaterials #materials
@natprot.nature.com
🧪 #2Dmaterials #materials
Protocols for advanced materials
A collection of articles covering protocols for synthesizing, processing and characterizing advanced materials, with particular emphasis on 2D materials and ...
www.nature.com
September 17, 2026 at 11:04 AM
In this collection we highlight protocols for synthesizing, processing and characterizing advanced materials, with particular emphasis on 2D materials and nanoscale materials.
@natprot.nature.com
🧪 #2Dmaterials #materials
@natprot.nature.com
🧪 #2Dmaterials #materials
This collection of protocols covers approaches for sensing using molecular, optical or electrochemical materials adopted for applications in imaging, trace analyte detection and organ-on-chip monitoring. @natprot.nature.com
🧪 #sensors #chemsky #materials
🧪 #sensors #chemsky #materials
Protocols for sensing
Sensing technologies provide us with the ability to detect changes in local chemical, biological or physical environments we would otherwise be unaware of. ...
www.nature.com
September 17, 2026 at 11:02 AM
This collection of protocols covers approaches for sensing using molecular, optical or electrochemical materials adopted for applications in imaging, trace analyte detection and organ-on-chip monitoring. @natprot.nature.com
🧪 #sensors #chemsky #materials
🧪 #sensors #chemsky #materials
New collection! @natprot.nature.com covering approaches at the interface of electronics, robotics and biological systems. The protocols cover how to design and fabricate robots, actuators and sensors using biological, magnetic and electronic building tools.
🧪 #bioelectronics
🧪 #bioelectronics
Protocols for bioelectronics
A collection of protocols at the interface of electronic, robotic and living systems to fabricate actuators, circuits, robots, wearables and integrated ...
www.nature.com
September 17, 2026 at 11:00 AM
New collection! @natprot.nature.com covering approaches at the interface of electronics, robotics and biological systems. The protocols cover how to design and fabricate robots, actuators and sensors using biological, magnetic and electronic building tools.
🧪 #bioelectronics
🧪 #bioelectronics
Big thank you to @munichimaging.bsky.social and @helmholtzmunich.bsky.social for the hospitality. I had the opportunity to meet with ECRs and PIs to discuss about careers, research publishing and how to incorporate protocols into their strategy.
#tech #ai #postdocs #students #juniorpis | Thomas Schwarz-Romond
Scientific publishing - in times of tremendous #tech acceleration and #AI becomes increasingly complex. Giancarlo Porcella Helmholtz PioneerCampus @ Helmholtz Munich recently hosted an editorial round...
lnkd.in
September 17, 2026 at 10:21 AM
Big thank you to @munichimaging.bsky.social and @helmholtzmunich.bsky.social for the hospitality. I had the opportunity to meet with ECRs and PIs to discuss about careers, research publishing and how to incorporate protocols into their strategy.
netneurotools is a Python toolkit that has been continuously developed by lab trainees as a set of tools to navigate the complex informatics ecosystem of brain imaging. @natprot.nature.com @misicbata.bsky.social
🧪 🧠 #imaging
🧪 🧠 #imaging
netneurotools: a trainee-oriented approach to network neuroscience - Nature Protocols
This is a Perspective covering a Python toolbox for neuroscience research that enables users to fetch datasets, construct networks and null models, estimate graph metrics, fit statistical models and v...
www.nature.com
September 15, 2026 at 6:40 AM
netneurotools is a Python toolkit that has been continuously developed by lab trainees as a set of tools to navigate the complex informatics ecosystem of brain imaging. @natprot.nature.com @misicbata.bsky.social
🧪 🧠 #imaging
🧪 🧠 #imaging
A nanosheet superspreading alignment strategy for fabricating nanocomposite films that uses shear-flow forces between two immiscible phases to induce long-range, high-order alignment of 2D nanosheets to enhance the films’ mechanical properties. @natprot.nature.com
🧪 #materials #chemsky #sensors
🧪 #materials #chemsky #sensors
Shear-flow-induced assembly of 2D nanosheets for the fabrication of composite films with high tensile strength - Nature Protocols
We present a protocol for the fabrication of highly ordered composite films in which 2D nanosheets can be aligned and fixed, yielding high tensile strength and suitability for high-performance wearabl...
www.nature.com
September 1, 2026 at 7:31 AM
A nanosheet superspreading alignment strategy for fabricating nanocomposite films that uses shear-flow forces between two immiscible phases to induce long-range, high-order alignment of 2D nanosheets to enhance the films’ mechanical properties. @natprot.nature.com
🧪 #materials #chemsky #sensors
🧪 #materials #chemsky #sensors
Radioafterglow nanoprobes synthesized by co-assembling radioabsorbers, radiosensitizers and radioafterglow substrates into amphiphilic polymers using the film rehydration method. @natprot.nature.com
🧪 🔬 #imaging #chemsky
🧪 🔬 #imaging #chemsky
Synthesis of radioafterglow nanoprobes for deep-tissue imaging of hydrogen peroxide - Nature Protocols
This protocol is for the synthesis of radioafterglow nanoprobes that convert X-ray energy to near-infrared emissions with long half-life and high brightness, detectable from up to 15-cm depth.
www.nature.com
September 1, 2026 at 7:25 AM
Radioafterglow nanoprobes synthesized by co-assembling radioabsorbers, radiosensitizers and radioafterglow substrates into amphiphilic polymers using the film rehydration method. @natprot.nature.com
🧪 🔬 #imaging #chemsky
🧪 🔬 #imaging #chemsky
A Protocol for MetaboAnalyst 6.0 which provides instructions for four key tasks in liquid chromatography–tandem mass spectrometry-based exposomics data analysis. @natprot.nature.com
🧪 ⚗️ #chemsky #metabolomics
🧪 ⚗️ #chemsky #metabolomics
August 20, 2026 at 9:35 AM
A Protocol for MetaboAnalyst 6.0 which provides instructions for four key tasks in liquid chromatography–tandem mass spectrometry-based exposomics data analysis. @natprot.nature.com
🧪 ⚗️ #chemsky #metabolomics
🧪 ⚗️ #chemsky #metabolomics
A Protocol covering the pathological assessment of paired tumor–blood DNA samples, developed by the Mutographs project and suitable for international genomics initiatives @who.int International Agency for Research on Cancer (IARC) @natprot.nature.com
🧪 🔬 #pathology #medsky #genomics
🧪 🔬 #pathology #medsky #genomics
Centralized processing of frozen tumor tissues for global cancer genomics - Nature Protocols
This Protocol outlines a pathologist-led, centralized workflow for processing frozen tumor tissues to generate high-quality DNA for whole-genome sequencing. It integrates cryosectioning, digital patho...
www.nature.com
August 20, 2026 at 9:20 AM
A Protocol covering the pathological assessment of paired tumor–blood DNA samples, developed by the Mutographs project and suitable for international genomics initiatives @who.int International Agency for Research on Cancer (IARC) @natprot.nature.com
🧪 🔬 #pathology #medsky #genomics
🧪 🔬 #pathology #medsky #genomics
One-step nanoscale expansion microscopy combines #ExM with fluctuation-based super-resolution analysis to enable the direct visualization of individual protein shapes, using conventional fluorescence microscopes. @natprot.nature.com 🧪 🔬 #imaging @alishaib.bsky.social
ONE microscopy - Nature Protocols
An approach for combining expansion microscopy with fluctuation-based super-resolution analysis enables the visualization of individual proteins via conventional fluorescence microscopy.
www.nature.com
August 11, 2026 at 2:59 PM
One-step nanoscale expansion microscopy combines #ExM with fluctuation-based super-resolution analysis to enable the direct visualization of individual protein shapes, using conventional fluorescence microscopes. @natprot.nature.com 🧪 🔬 #imaging @alishaib.bsky.social
Fabrication of optical #metasurfaces via a printable high-index composite material known as nanoparticle-embedded resin (nanoPER) supports real-world applications such as light detection and ranging, compact imaging and integrated #photonics @natprot.nature.com
🧪 #materials #nanotechnology
🧪 #materials #nanotechnology
Direct printing of metasurfaces using formulated optical materials - Nature Protocols
A printable composite material provides a practical and cost-effective route for fabricating metasurfaces on flexible or curved substrates, scalable from the UV to IR wavelengths and deployable for ph...
www.nature.com
August 11, 2026 at 2:35 PM
Fabrication of optical #metasurfaces via a printable high-index composite material known as nanoparticle-embedded resin (nanoPER) supports real-world applications such as light detection and ranging, compact imaging and integrated #photonics @natprot.nature.com
🧪 #materials #nanotechnology
🧪 #materials #nanotechnology
A #protocol for the reliable and reproducible evaluation of antioxidant nanozymes to facilitate direct comparison across materials, elucidating structure–activity relationships and their rational optimization for biomedical applications. @natprot.nature.com
🧪 ⚗️ #chemsky
🧪 ⚗️ #chemsky
Standardized assays for evaluating superoxide dismutase-like and catalase-like activities of nanozymes - Nature Protocols
Antioxidant nanozymes are therapeutic candidates for oxidative diseases. Here a standardized protocol is described to characterize superoxide dismutase-like or catalase-like nanozymes and quantify the...
www.nature.com
August 11, 2026 at 2:14 PM
A #protocol for the reliable and reproducible evaluation of antioxidant nanozymes to facilitate direct comparison across materials, elucidating structure–activity relationships and their rational optimization for biomedical applications. @natprot.nature.com
🧪 ⚗️ #chemsky
🧪 ⚗️ #chemsky
I missed this post was out, check out these profiles of young American scientists!
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Meet our inaugural class of Young American Scientists: 28 researchers who are redefining the future of science. sciam.com/youngscientists
August 7, 2026 at 9:02 AM
I missed this post was out, check out these profiles of young American scientists!
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A protocol for a general method for synthesizing nanoisland catalysts in which nanoscale oxide islands are isolated on high-surface-area substrates to confine and stabilize metal species. @natprot.nature.com
🧪 ⚗️ #catalysis #chemsky #materials
🧪 ⚗️ #catalysis #chemsky #materials
A general approach for the synthesis of nanoisland catalysts - Nature Protocols
Stabilization of metal single-atom or cluster catalysts is achieved by forming CeOx nanoislands on SiO2 through strong electrostatic adsorption method. Transition-metal precursors are added to form si...
www.nature.com
August 6, 2026 at 8:11 AM
A protocol for a general method for synthesizing nanoisland catalysts in which nanoscale oxide islands are isolated on high-surface-area substrates to confine and stabilize metal species. @natprot.nature.com
🧪 ⚗️ #catalysis #chemsky #materials
🧪 ⚗️ #catalysis #chemsky #materials
#Core2Edge, a human glioblastoma organoid and brain slice co-culture model that recapitulates tumor infiltration and transcriptional heterogeneity. The protocol also includes a light-sheet imaging approach to capture tumor network morphology in three dimensions. @natprot.nature.com
🧪 🔬 🧠 #cancer
🧪 🔬 🧠 #cancer
Core2Edge: a human glioblastoma organoid–brain slice model capturing infiltration and transcriptional heterogeneity from core to single-cell dispersion - Nature Protocols
This protocol describes Core2Edge, a human glioblastoma organoid and brain slice co-culture model that recapitulates tumor infiltration and transcriptional heterogeneity, along with a light-sheet imag...
www.nature.com
July 29, 2026 at 2:55 PM
#Core2Edge, a human glioblastoma organoid and brain slice co-culture model that recapitulates tumor infiltration and transcriptional heterogeneity. The protocol also includes a light-sheet imaging approach to capture tumor network morphology in three dimensions. @natprot.nature.com
🧪 🔬 🧠 #cancer
🧪 🔬 🧠 #cancer
This is a protocol extension for a programmable tetrahedral DNA nanostructure (TDN) platform that enables precise spatial control of capture ligands through site-specific editability. @natprot.nature.com
🧪 ⚗️ #chemsky #nanotechnology
🧪 ⚗️ #chemsky #nanotechnology
A programmable DNA tetrahedron platform for selective and efficient capture of cells and proteins - Nature Protocols
This Protocol Extension describes a programmable tetrahedral DNA nanostructure (TDN) platform for selective cell and protein capture, using aptamer-functionalized TDN for cell capture and peptide-func...
www.nature.com
July 28, 2026 at 1:05 PM
This is a protocol extension for a programmable tetrahedral DNA nanostructure (TDN) platform that enables precise spatial control of capture ligands through site-specific editability. @natprot.nature.com
🧪 ⚗️ #chemsky #nanotechnology
🧪 ⚗️ #chemsky #nanotechnology
A protocol for a focus-feedback microscopy algorithm that enables the accurate tracking of structures within a single optical plane over time, thereby overcoming the limitations of traditional z-stack imaging and reducing imaging intervals and light exposure. @natprot.nature.com
🧪 🔬 #imaging
🧪 🔬 #imaging
Three-dimensional tracking of dynamic structures in living cells using single-plane imaging with focus feedback - Nature Protocols
A microscopy approach for the use of focus-feedback for single-plane tracking of subcellular structures during prolonged live-cell fluorescent imaging sessions.
www.nature.com
July 28, 2026 at 12:44 PM
A protocol for a focus-feedback microscopy algorithm that enables the accurate tracking of structures within a single optical plane over time, thereby overcoming the limitations of traditional z-stack imaging and reducing imaging intervals and light exposure. @natprot.nature.com
🧪 🔬 #imaging
🧪 🔬 #imaging
Polymer deconstruction is part of plastic waste management. These transformations generate multiphase mixtures of species in the gas, liquid and solid phases which require accurate, quantitative measurements of their distributions. @natprot.nature.com
🧪 #chemsky #catalyis
🧪 #chemsky #catalyis
Comprehensive quantitative analysis of polyolefin hydrogenolysis toward plastic waste management - Nature Protocols
Polymer deconstruction, via catalytic hydrogenolysis, is an important approach for plastic recycling and upcycling. This workflow describes sequential quantitative analysis using multiple techniques t...
www.nature.com
July 27, 2026 at 11:24 AM
Polymer deconstruction is part of plastic waste management. These transformations generate multiphase mixtures of species in the gas, liquid and solid phases which require accurate, quantitative measurements of their distributions. @natprot.nature.com
🧪 #chemsky #catalyis
🧪 #chemsky #catalyis
A collection of protocols covering a wide range of immunological-based assays and methods adapted for immunological research published @natprot.nature.com
🧪 #medsky #immunosky
Take a look:
🧪 #medsky #immunosky
Take a look:
Protocols for immunological research
A collection of protocols covering a range of techniques for isolating, profiling, identifying and characterizing immune cells and biomarkers
www.nature.com
July 25, 2026 at 7:01 AM
A collection of protocols covering a wide range of immunological-based assays and methods adapted for immunological research published @natprot.nature.com
🧪 #medsky #immunosky
Take a look:
🧪 #medsky #immunosky
Take a look: