This long-term genomic surveillance identifies a widely disseminated carbapenem-resistant Klebsiella pneumoniae (CRKP) sublineage with enhanced invasiveness but no increased mortality, revealing immun...

Here, we describe a putatively hypervirulent, carbapenem-resistant Klebsiella pneumoniae (hv-CRKP) outbreak. Nanopore sequencing guided the infection prevention and control (IPC) responses during the ...

WHO recently updated the list of bacterial priority pathogens, categorising carbapenem-resistant Klebsiella pneumoniae (CRKP) among the critical group.1 In this regard, the endemic status of CRKP has ...

Carbapenem-resistant Klebsiella pneumoniae (CRKP), a WHO’s critical priority pathogen, continuously evolves to generate health-threatening high-risk (sub)lineages. Here, we conducted a 10-year surveillance of nosocomial CRKP infections, collecting and whole-genome sequencing 1513 clinical isolates, accompanied by clinical data. We applied fine-scale genome analysis for 60,724 non-local public-available K. pneumoniae genomes. We identified a predominant ST11-KL64 sublineage widely-disseminated across China and internationally-distributed. Isolates of this sublineage were more frequently recovered during seasonal influenza peaks and harbored plasmids encoding ‘hypervirulence’ factors but caused no increase in patient mortality. Instead, they exhibited enhanced invasiveness and translocation capacity. Mechanistically, this suggested highly invasive CRKP (hiCRKP) sublineage showed elevated resistance to macrophage-mediated phagocytosis, partly due to the virulence-encoding plasmid and the

Carbapenem-resistant Klebsiella pneumoniae (CRKP) poses critical threats to public health, with sequence type 11-capsular type 64 (ST11-KL64) CRKP dom…

YouTube video by Alphablocks

Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a difficult-to-treat pathogen. Phages recovered by a “stepwise” approach may constitute a cocktail able to prolong retardation of bacterial regr...

Author summary Carbapenem resistant K. pneumoniae (CRKP) have emerged as a cause of hospital and community acquired infections, posing a significant threat to public health, and have hence been listed...

WHO recently updated the list of bacterial priority pathogens, categorising carbapenem-resistant Klebsiella pneumoniae (CRKP) among the critical group.1 In this regard, the endemic status of CRKP has ...

IntroductionCarbapenem-resistant Klebsiella pneumoniae (CRKP) skin infections represent a critical therapeutic challenge due to biofilm formation and poor dr...

YouTube video by The Oregonian

Carbapenem-resistant Enterobacteriaceae (CRE) have been classified as "urgent priority pathogens" due to their high drug resistance and wide spread. Among them, carbapenem-resistant Escherichia coli (CREco) and carbapenem-resistant Klebsiella pneumoniae (CRKP) are the main types. Phages are naturall …

The emergence of Carbapenem-resistant Klebsiella pneumoniae (CRKP) represents a major public health concern, primarily driven by its ability to evade a wide range of antibiotics. Despite extensive genomic studies, proteomic insights into antibiotic resistance mechanisms remain scarce. Here, we employed a Data-Independent Acquisition (DIA)-based quantitative proteomics approach to investigate proteomic differences between 78 CRKP and 18 Carbapenem-sensitive K. pneumoniae (CSKP) clinical isolates. A total of 3380 proteins were identified, with 946 showing significant differential expression. CRKP isolates exhibited increased expression of efflux pumps, beta-lactamases, and transcriptional regulators, while proteins associated with transport were enriched in CSKP isolates. To validate our findings, a quantitative proteomics analysis in an independent cohort of 10 CRKP and 11 CSKP isolates was performed. The key biomarkers identified via machine learning in the discovery cohort, including aldehyde dehydrogenase (KPN_03361), acyltransferase (KPN_02072), uncharacterized protein (YjeJ), plasmid partition protein B (ParaB), HTH-type transcriptional activator (RhaR), and beta-lactamase (Bla), were evaluated. They collectively achieved AUC > 0.7 in the validation cohort, confirming their discriminatory capacity as diagnostic markers. These findings provide novel insights into the molecular mechanisms of antibiotic resistance and identify promising biomarkers for diagnosing carbapenem-resistant K. pneumoniae, offering potential avenues for therapeutic intervention.

AbstractBackground. Factors contributing to the success of carbapenem-resistant Klebsiella pneumoniae (CRKP) of sequence type 11 (ST11) remain poorly under

These findings showed bacteriophages and phage cocktails with high specificity against CRKP belonging to the successful clones CG258 and ST307 with promising characteristics, making them an alternative for controlling these clones in different environmental or health settings, biocontrol agents, or …

Read the latest articles of International Journal of Antimicrobial Agents at ScienceDirect.com, Elsevier’s leading platform of peer-reviewed scholarly literature

The isolation rate of Klebsiella pneumoniae (K. pneumoniae), particularly carbapenem-resistant K. pneumoniae (CRKP), is increasing in the food product…

Klebsiella pneumoniae (K. pneumoniae) is a non-motile Gram-negative bacterium in the Enterobacteriaceae family. The rise of antibiotic resistance, esp…