I’m doing high-dimensional single-cell proteomics
Aka flow cytometry
Aka flow cytometry
June 13, 2025 at 4:18 PM
I’m doing high-dimensional single-cell proteomics
Aka flow cytometry
Aka flow cytometry
Did you ever wonder what insights hide in the multiplets that are usually discarded in flow cytometry? 🫧 Find out in the first publication of my PhD, now out in Nature Methods! www.nature.com/articles/s41... (1/4)
August 7, 2025 at 8:48 PM
Did you ever wonder what insights hide in the multiplets that are usually discarded in flow cytometry? 🫧 Find out in the first publication of my PhD, now out in Nature Methods! www.nature.com/articles/s41... (1/4)
As you might know, I use ImageTwin, a software tool that can find duplications within and between papers. It has a large database of images.
Here, it found something a human would probably never find: A group from China possibly stealing flow cytometry images from a group from Japan.
Here, it found something a human would probably never find: A group from China possibly stealing flow cytometry images from a group from Japan.
November 18, 2024 at 1:20 AM
As you might know, I use ImageTwin, a software tool that can find duplications within and between papers. It has a large database of images.
Here, it found something a human would probably never find: A group from China possibly stealing flow cytometry images from a group from Japan.
Here, it found something a human would probably never find: A group from China possibly stealing flow cytometry images from a group from Japan.
Huge credit to @colibricytometry.bsky.social, who has spent years working on every aspect of the spectral flow cytometry pipeline. Follow the Colibri blog to get tips in real-time, pre-pre-print!
www.colibri-cytometry.com
www.colibri-cytometry.com
Cytometry Colibri | flow cytometry
Cytometry Colibri offers scientific consulting, data analysis, panel design, method development, tutoring and all things related to flow cytometry.
Expertise in high parameter flow cytometry, spectra...
www.colibri-cytometry.com
October 28, 2025 at 8:06 AM
Huge credit to @colibricytometry.bsky.social, who has spent years working on every aspect of the spectral flow cytometry pipeline. Follow the Colibri blog to get tips in real-time, pre-pre-print!
www.colibri-cytometry.com
www.colibri-cytometry.com
Mass cytometry with signal amplification enables measurement of low-abundance proteins go.nature.com/3YjKiol
rdcu.be/em32L
rdcu.be/em32L
Signal amplification by cyclic extension enables high-sensitivity single-cell mass cytometry - Nature Biotechnology
Mass cytometry with signal amplification enables measurement of low-abundance proteins.
go.nature.com
June 6, 2025 at 4:43 AM
Mass cytometry with signal amplification enables measurement of low-abundance proteins go.nature.com/3YjKiol
rdcu.be/em32L
rdcu.be/em32L
Just saw the typo "flaw cytometry" in an article about fraudulent flow cytometry and it is perfect.
January 9, 2024 at 7:18 PM
Just saw the typo "flaw cytometry" in an article about fraudulent flow cytometry and it is perfect.
When I’m on cytometry they call me the Flow Rider
December 15, 2024 at 9:35 PM
When I’m on cytometry they call me the Flow Rider
Interact-omics is a high-throughput cytometry-based framework for cellular interaction mapping. @simonhaas.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
August 7, 2025 at 7:53 PM
Interact-omics is a high-throughput cytometry-based framework for cellular interaction mapping. @simonhaas.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Very cool talk on Tips and Tricks on Spectral Flow Cytometry by Dr. Oliver Burton (@labliston.bsky.social) in the ThemeNight of the Flow Cytometry Core Facility of the @unibonn.bsky.social!
December 1, 2025 at 4:51 PM
Very cool talk on Tips and Tricks on Spectral Flow Cytometry by Dr. Oliver Burton (@labliston.bsky.social) in the ThemeNight of the Flow Cytometry Core Facility of the @unibonn.bsky.social!
Can you spot the troubles with these flow cytometry panels?
#ImageForensics
#ImageForensics
December 31, 2024 at 12:23 AM
Can you spot the troubles with these flow cytometry panels?
#ImageForensics
#ImageForensics
Michigan snow is so much better for your flow cytometry ice bucket, no slushiness in right bucket.
#immunosky #dermsky
#immunosky #dermsky
January 17, 2025 at 2:43 PM
Michigan snow is so much better for your flow cytometry ice bucket, no slushiness in right bucket.
#immunosky #dermsky
#immunosky #dermsky
You can also save money by cutting-costs and preventing waste. We do a low of flow cytometry, and retitrated antibodies for overnight staining, reducing costs ~100-fold while improving signal-to-noise and reproducibility
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
Do more with Less: Improving High Parameter Cytometry Through Overnight Staining
Recent advances in flow cytometry have allowed high-dimensional characterization of biological phenomena, enabling breakthroughs in a multitude of fields. Despite the appreciation of the unique prope...
currentprotocols.onlinelibrary.wiley.com
September 3, 2025 at 4:03 PM
You can also save money by cutting-costs and preventing waste. We do a low of flow cytometry, and retitrated antibodies for overnight staining, reducing costs ~100-fold while improving signal-to-noise and reproducibility
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...
I am excited to share that our imaging flow cytometry microclot analysis is now done in Europe! The Swiss lab INUS Laboratories AG has the licence!
inus.health/en/lab/
inus.health/en/lab/
February 7, 2025 at 8:27 PM
I am excited to share that our imaging flow cytometry microclot analysis is now done in Europe! The Swiss lab INUS Laboratories AG has the licence!
inus.health/en/lab/
inus.health/en/lab/
Hi #Botany2025 - In my quest to never do flow cytometry again, I am collecting sequence data (radseq, target enrichment, WGS, ect.) with confirmed ploidal level. If you have samples, reach out!
July 30, 2025 at 4:34 PM
Hi #Botany2025 - In my quest to never do flow cytometry again, I am collecting sequence data (radseq, target enrichment, WGS, ect.) with confirmed ploidal level. If you have samples, reach out!
42-Parameter (40-Color) Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Human Peripheral Blood Leukocytes
doi.org/10.1002/cyto...
doi.org/10.1002/cyto...
March 18, 2025 at 4:36 PM
42-Parameter (40-Color) Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Human Peripheral Blood Leukocytes
doi.org/10.1002/cyto...
doi.org/10.1002/cyto...
A small 🧵 encouraging people to publish protocols.
Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work.
www.biorxiv.org/content/10.1...
Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work.
www.biorxiv.org/content/10.1...
AutoSpectral improves spectral flow cytometry accuracy through optimised spectral unmixing and autofluorescence-matching at the cellular level
The advent of spectral flow cytometry has seen a rapid rise in the complexity of flow cytometry experiments, allowing the construction of assays with at least 50 fluorescent parameters. To correctly d...
www.biorxiv.org
October 31, 2025 at 9:09 AM
A small 🧵 encouraging people to publish protocols.
Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work.
www.biorxiv.org/content/10.1...
Our #AutoSpectral release has made me reflect on how much the community tends to appreciate protocol papers. People rarely present protocols as their main achievements, but they are often the most impactful work.
www.biorxiv.org/content/10.1...
Single-molecule flow cytometry https://www.biorxiv.org/content/10.1101/2025.08.26.672174v1
August 27, 2025 at 2:48 AM
Single-molecule flow cytometry https://www.biorxiv.org/content/10.1101/2025.08.26.672174v1
A single-cell mass cytometry-based atlas of the developing mouse brain 🧪🧠
www.nature.com/articles/s41...
www.nature.com/articles/s41...
A single-cell mass cytometry-based atlas of the developing mouse brain - Nature Neuroscience
In recent years, valuable mRNA-based developmental atlases of the mouse brain have been made available. Here, the authors use single-cell mass cytometry to build a protein-based single-cell profiling ...
www.nature.com
January 2, 2025 at 3:28 PM
A single-cell mass cytometry-based atlas of the developing mouse brain 🧪🧠
www.nature.com/articles/s41...
www.nature.com/articles/s41...
fcs, the format used for flow cytometry machines, is a completely cursed 1980s data format, and tmk it only renders via FlowJo, which is proprietary software with awful licensing terms.
anyway i'm going to render 4OJ72_males.fcs exactly the same way as FlowJo.
zenodo.org/records/7390...
anyway i'm going to render 4OJ72_males.fcs exactly the same way as FlowJo.
zenodo.org/records/7390...
Raw flow cytometry data files (fcs-files)
These FCS files are a part of the supplementary data for the following publication: Meiotic transmission patterns of additional genomic elements in Brachionus asplanchnoidis, a rotifer with intraspeci...
zenodo.org
August 20, 2026 at 4:00 AM
fcs, the format used for flow cytometry machines, is a completely cursed 1980s data format, and tmk it only renders via FlowJo, which is proprietary software with awful licensing terms.
anyway i'm going to render 4OJ72_males.fcs exactly the same way as FlowJo.
zenodo.org/records/7390...
anyway i'm going to render 4OJ72_males.fcs exactly the same way as FlowJo.
zenodo.org/records/7390...
Cute Girls do Flow Cytometry. Finally.
August 23, 2025 at 3:20 PM
Cute Girls do Flow Cytometry. Finally.
New paper alert 💥 "Spectral biophysical cytometry with nanosensors reveals remodelling of immune cells in atherosclerosis"
Open access paper here: www.nature.com/articles/s41...
Open access paper here: www.nature.com/articles/s41...
Spectral biophysical cytometry with nanosensors reveals remodelling of immune cells in atherosclerosis - Nature Nanotechnology
Membrane-permeant, environment-sensitive nanosensors enable high-throughput, multiparametric quantification of membrane order and mitochondrial potential, revealing immune-cell-specific remodelling an...
www.nature.com
August 14, 2026 at 9:01 AM
New paper alert 💥 "Spectral biophysical cytometry with nanosensors reveals remodelling of immune cells in atherosclerosis"
Open access paper here: www.nature.com/articles/s41...
Open access paper here: www.nature.com/articles/s41...
November 15, 2025 at 3:08 AM
...but I am now banned from every HPLC, GC/MS, flow cytometry and microscopy lab in a five-county area.
October 22, 2025 at 9:26 PM
...but I am now banned from every HPLC, GC/MS, flow cytometry and microscopy lab in a five-county area.
Postdoc vacancy: Microbial dormancy in the cryosphere
@erc.europa.eu #ERC_SIESTA
📢 PLEASE RT
🧬 Single cell microbial activity measurements, flow cytometry, cell sorting, omics, ecological interpretation
☀️ Marseille, France
‼️ Apply ASAP & before 7 Nov
Link: emploi.cnrs.fr/Offres/CDD/U...
@erc.europa.eu #ERC_SIESTA
📢 PLEASE RT
🧬 Single cell microbial activity measurements, flow cytometry, cell sorting, omics, ecological interpretation
☀️ Marseille, France
‼️ Apply ASAP & before 7 Nov
Link: emploi.cnrs.fr/Offres/CDD/U...
October 21, 2025 at 3:48 PM
Postdoc vacancy: Microbial dormancy in the cryosphere
@erc.europa.eu #ERC_SIESTA
📢 PLEASE RT
🧬 Single cell microbial activity measurements, flow cytometry, cell sorting, omics, ecological interpretation
☀️ Marseille, France
‼️ Apply ASAP & before 7 Nov
Link: emploi.cnrs.fr/Offres/CDD/U...
@erc.europa.eu #ERC_SIESTA
📢 PLEASE RT
🧬 Single cell microbial activity measurements, flow cytometry, cell sorting, omics, ecological interpretation
☀️ Marseille, France
‼️ Apply ASAP & before 7 Nov
Link: emploi.cnrs.fr/Offres/CDD/U...