#DeepMutationalScanning
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varianteffect.bsky.social
Our final speaker before morning coffee is Doug Fowler from the University of Washington.

His talk details pathway-scale variant effect mapping in human cells with LABEL-seq.

#VariantEffect26
#LABELseq ##DeepMutationalScanning
#AtlasOfVariantEffectsAlliance
March 27, 2026 at 12:04 AM
varianteffect.bsky.social
Critical Assessment of Genome Interpretation (CAGI) challenges are live! 🧬💻
genomeinterpretation.org/cagi7-challe...
A total of 12 challenges have launched Including #DeepMutationalScanning Challenges
⏰ Deadlines approaching! #CAGI #GenomeInterpretation #VariantEffect
August 29, 2025 at 2:36 PM
varianteffect.bsky.social
You can now find the Day 2 presentations from the MSS 2026 over on our YouTube channel. ⏯️ bit.ly/4tzo9hQ

The final day's talks will be live soon, so watch this space!

#VariantEffect26 #Genomics #DeepMutationalScanning #FunctionalGenomics #AtlasOfVariantEffects
May 13, 2026 at 7:58 AM
ptdolan.bsky.social
www.nature.com/articles/s41...

Excited to have this collaboration from #QVEU and @rongellerlab.bsky.social lab out in the world! After publishing our own #DeepMutationalScanning studies on two related viruses, we asked what we learn from comparisons of such datasets. The answer is a lot!
Comparative analysis of deep mutational scanning datasets in enteroviruses A and B identifies functional divergence and therapeutic targets - Nature Ecology & Evolution
A comparison of proteome-wide mutational scanning datasets from enterovirus A and B species reveals evolutionary constraints shared by these viral species that localize to core functional regions, as ...
www.nature.com
February 24, 2026 at 10:44 PM
varianteffect.bsky.social
Opening the final day of MSS 2026 is Maitreya Dunham from the University of Washington. Her keynote is entitled, 'From ARS1 to ZWF1: some adventures in deep mutational scanning'.

#VariantEffect26
#DeepMutationalScanning
#AtlasOfVariantEffectsAlliance
March 26, 2026 at 10:17 PM
plosbiology.org
#DeepMutationalScanning (DMS) experiments are limited by gene size due to library complexity & costs. @christianlandry.bsky.social &co develop an efficient & cost-effective barcoded cloning strategy for plasmid-based DMS libraries that enables study of large genes @plosbiology.org 🧪 plos.io/4abhyUf
Strategy to generate a DMS plasmid library for Your Favorite Gene (YFG) using short, degenerate libraries. 1. Segmentation of YFG into sub-fragments, each fragment corresponding to a DNA region to be synthesized. The same approach can be applied to promoter and terminator regions, if desired. 2. Example of a pool of degenerate oligonucleotides (oPool) derived from one YFG fragment associated with DNA barcodes. Each oPool contains: (i) ~40 bp of homology upstream of the YFG fragment of interest, (ii) the YFG fragment sequence with a single NNK codon, (iii) BsaI cloning sites, (iv) a DNA barcode composed of codon-position specific regions and six degenerate nucleotides (N), and (v) a conserved i7 primer binding site (PBS_i7) present in all oPools and used for rapid and efficient sequencing library preparation. Current oligonucleotide synthesis technologies allow for a total of nine degenerate positions per fragment: three are used for the degenerate codon (NNK), and six for the barcode. A complete list of all oPool sequences and their detailed composition is provided in S1 Table. 3. Protocol for constructing YFG DMS plasmid library from oPools using two cloning steps that maintain the physical barcode-mutation association. The libraries of oPools are cloned into the plasmid template by Gibson cloning. Following this step, for each fragment, a necessary short-read sequencing using PBS_i5 (included in the 5′ sequencing primer) and PBS_i7 is performed to associate each barcode with its corresponding mutation and to assess both barcode diversity per mutation and mutation coverage for the whole fragment. The ultimate step consists in Golden Gate cloning of the missing 3′ gene fragment between the degenerate fragment and the barcode. An additional short-read sequencing step of the barcodes can be performed to make sure that coverage and diversity have been maintained. Figure created in BioRender.
February 11, 2026 at 7:19 PM
varianteffect.bsky.social
We're now joined by Max Stammnitz from the Centre for Genomic Regulation (CRG) in Barcelona. His presentation is about the genetic architecture of allosteric plant hormone receptors.

#VariantEffect26
#DeepMutationalScanning
#AtlasOfVariantEffectsAlliance
March 26, 2026 at 11:31 PM
varianteffect.bsky.social
Next to speak is Xinyu Wu from WEHI (the Walter and Eliza Hall Institute of Medical Research). His talk discusses the elucidation of distinct conformations in thrombopoietin receptor activation.

#VariantEffect26
#DeepMutationalScanning
#AtlasOfVariantEffectsAlliance
March 26, 2026 at 5:41 AM
varianteffect.bsky.social
Tomorrow, our Variant Effect Seminar speakers will be covering various aspects of the modeling of variant effects. They hope you can join them!

Details here: www.varianteffect.org/seminar-seri...

#Genomics #VariantInterpretation #DeepMutationalScanning #AtlasOfVariantEffects
May 5, 2026 at 7:56 AM
varianteffect.bsky.social
Don't miss Abbye McEwen's deep dive into interpretation of variants of unknown significance (VUS) - happening today (12.00 pm ET / 9.00 am PT / 5.00 pm BST).

Join here: bit.ly/3Seq0LL

#DeepMutationalScanning #VariantScientistNetwork #AtlasOfVariantEffects
June 17, 2026 at 8:54 AM
varianteffect.bsky.social
Now, we welcome Rosa De Santis of the Telethon Institute of Genetics and Medicine (TIGEM) to the podium. Her talk covers bulk to single-cell: benchmarking analytical tools for deep mutational scanning experiments.

#VariantEffect26
#DeepMutationalScanning
#AtlasOfVariantEffectsAlliance
March 26, 2026 at 4:26 AM
varianteffect.bsky.social
This Wednesday, are you ready to go beyond the VUS and learn more about using multiplexed assays of variant effects (MAVEs) to inform clinical variant classification?

It's free to sign up: bit.ly/3Seq0LL

#DeepMutationalScanning #VariantScientistNetwork #VUS #MAVE #AtlasOfVariantEffects
June 15, 2026 at 10:44 AM
ptdolan.bsky.social
Jack Dorman, @williambakhache.bsky.social and I wrote a review of #DeepMutationalScanning ( #DMS ) applications in #virology, exploring the ways it’s been used to understand the rules of #virus #evolution and #immunity. We also explore new applications of the technology. Check it out!
Scanning the horizon: deep mutational scanning approaches in virology | Journal of Virology
The development of deep mutational scanning (DMS) approaches has led to a fundamental shift in our ability to connect viral genotype and phenotype (1) and accelerated our understanding of viral evolut...
journals.asm.org
July 6, 2026 at 9:32 AM
plosbiology.org
#DeepMutationalScanning (DMS) experiments are limited by gene size due to library complexity & costs. @christianlandry.bsky.social &co develop an efficient & cost-effective barcoded cloning strategy for plasmid-based DMS libraries that enables study of large genes @plosbiology.org 🧪 plos.io/4abhyUf
Strategy to generate a DMS plasmid library for Your Favorite Gene (YFG) using short, degenerate libraries. 1. Segmentation of YFG into sub-fragments, each fragment corresponding to a DNA region to be synthesized. The same approach can be applied to promoter and terminator regions, if desired. 2. Example of a pool of degenerate oligonucleotides (oPool) derived from one YFG fragment associated with DNA barcodes. Each oPool contains: (i) ~40 bp of homology upstream of the YFG fragment of interest, (ii) the YFG fragment sequence with a single NNK codon, (iii) BsaI cloning sites, (iv) a DNA barcode composed of codon-position specific regions and six degenerate nucleotides (N), and (v) a conserved i7 primer binding site (PBS_i7) present in all oPools and used for rapid and efficient sequencing library preparation. Current oligonucleotide synthesis technologies allow for a total of nine degenerate positions per fragment: three are used for the degenerate codon (NNK), and six for the barcode. A complete list of all oPool sequences and their detailed composition is provided in S1 Table. 3. Protocol for constructing YFG DMS plasmid library from oPools using two cloning steps that maintain the physical barcode-mutation association. The libraries of oPools are cloned into the plasmid template by Gibson cloning. Following this step, for each fragment, a necessary short-read sequencing using PBS_i5 (included in the 5′ sequencing primer) and PBS_i7 is performed to associate each barcode with its corresponding mutation and to assess both barcode diversity per mutation and mutation coverage for the whole fragment. The ultimate step consists in Golden Gate cloning of the missing 3′ gene fragment between the degenerate fragment and the barcode. An additional short-read sequencing step of the barcodes can be performed to make sure that coverage and diversity have been maintained. Figure created in BioRender.
February 12, 2026 at 1:55 PM
plosbiology.org
#DeepMutationalScanning (DMS) experiments are limited by gene size due to library complexity & costs. @christianlandry.bsky.social &co develop an efficient & cost-effective barcoded cloning strategy for plasmid-based DMS libraries that enables study of large genes @plosbiology.org 🧪 plos.io/4abhyUf
Strategy to generate a DMS plasmid library for Your Favorite Gene (YFG) using short, degenerate libraries. 1. Segmentation of YFG into sub-fragments, each fragment corresponding to a DNA region to be synthesized. The same approach can be applied to promoter and terminator regions, if desired. 2. Example of a pool of degenerate oligonucleotides (oPool) derived from one YFG fragment associated with DNA barcodes. Each oPool contains: (i) ~40 bp of homology upstream of the YFG fragment of interest, (ii) the YFG fragment sequence with a single NNK codon, (iii) BsaI cloning sites, (iv) a DNA barcode composed of codon-position specific regions and six degenerate nucleotides (N), and (v) a conserved i7 primer binding site (PBS_i7) present in all oPools and used for rapid and efficient sequencing library preparation. Current oligonucleotide synthesis technologies allow for a total of nine degenerate positions per fragment: three are used for the degenerate codon (NNK), and six for the barcode. A complete list of all oPool sequences and their detailed composition is provided in S1 Table. 3. Protocol for constructing YFG DMS plasmid library from oPools using two cloning steps that maintain the physical barcode-mutation association. The libraries of oPools are cloned into the plasmid template by Gibson cloning. Following this step, for each fragment, a necessary short-read sequencing using PBS_i5 (included in the 5′ sequencing primer) and PBS_i7 is performed to associate each barcode with its corresponding mutation and to assess both barcode diversity per mutation and mutation coverage for the whole fragment. The ultimate step consists in Golden Gate cloning of the missing 3′ gene fragment between the degenerate fragment and the barcode. An additional short-read sequencing step of the barcodes can be performed to make sure that coverage and diversity have been maintained. Figure created in BioRender.
February 12, 2026 at 9:00 AM
onehealthgenomics.bsky.social
New Publication:
Mechanistic modeling of recessive disease through allelic integration of variant effects

@uoe-igc.bsky.social

#functionalgenomics #varianteffects #deepmutationalscanning #recessivedisease #enzymebiochemistry

www.sciencedirect.com/science/arti...
Mechanistic modeling of recessive disease through allelic integration of variant effects
Interpreting variants in recessive diseases is difficult because clinical severity depends on the combined function of both alleles. Deep mutational s…
www.sciencedirect.com
June 26, 2026 at 2:10 PM
genomematt.bsky.social
Currently this is involving lots of #FunctionalGenomics with #MAVE assays and #DMS (#DeepMutationalScanning) to dissect protein complex interactions with inhibitors.
I mainly do #ComputationalBiology and supervise a team that does the wet work.
September 22, 2023 at 3:45 AM
wilbanks.bsky.social
I am obsessed over data providing real biological signal rather than technical artifacts (because machines can be terrible at distinguishing the two in data analysis!). Liked this recent data release from @alignbio.bsky.social as an example www.linkedin.com/posts/corey-...
#proteinml #openscience #deepmutationalscanning #aixbio #groqseq | Corey Hudson
Reproducibility in high-throughput biology is hard, and we wanted to take it seriously. A new preprint from our team at the Align Foundation, in collaboration with NIST-LMSF and the DAMP Lab: we ran G...
www.linkedin.com
April 20, 2026 at 2:28 PM
varianteffect.bsky.social
Save the date for April's Variant Effects Seminar, where we will be joined by two more speakers with talks covering cancer and cardiology topics.

#Genomics #CancerBiology #VariantInterpretation #DeepMutationalScanning #FunctionalGenomics #ResearchTalk #StructuralBiology #AtlasOfVariantEffect
Image describes the two talks upcoming in the April Variant Effect Seminar. Olivia Zhang's talk: KBTBD4 Cancer Hotspot Mutations Drive Neomorphic Degradation of HDAC1/2 Corepressor Complexes. Yuta Yamamoto's talk: Scaled multidimensional assays of variant effect for hypertrophic cardiomyopathy. Sign up via the QR code or at varianteffect.org/seminar-series.
March 16, 2026 at 12:28 PM
newsmedical.bsky.social
Groundbreaking method dmSLAY improves antimicrobial peptide effectiveness and safety 🔬🧬✨ www.news-medical.net/news/2024080... #Antimicrobial #BioEngineering #MachineLearning #DeepMutationalScanning #SyntheticBiology #Peptides #Microbiology #BioTech @natureportfolio.bsky.social
Groundbreaking method dmSLAY improves antimicrobial peptide effectiveness and safety
Researchers developed a high-throughput method, dmSLAY, to assess thousands of AMP variants, combining it with machine learning to enhance membrane selectivity and reduce toxicity.
www.news-medical.net
August 2, 2024 at 3:08 AM