#FluoTag
📣 HaloTag fans, meet 𝐅𝐥𝐮𝐨𝐓𝐚𝐠®-𝐗𝟒 𝐚𝐧𝐭𝐢-𝐇𝐚𝐥𝐨 – our new #sdAb -based fluorescent reagent for direct, bright & highly specific labeling of #HaloTag fusion proteins.

• 2 sdAb clones = amplified signal
• preserves HaloTag ligand binding

Learn more: bit.ly/3ULE5OK

#Nanobody #ProteinLabeling #Imaging
August 6, 2025 at 12:40 PM
sdABs licensed from @nanotag.bsky.social , where they're available unmodified and fluorophore-coupled (FluoTag®)
July 16, 2025 at 7:31 AM
Multiplexing with same-species primary antibodies, solved with Premix&Stain & Smart Secondary Nanobodies.

Actin · GFP tag · Golgi · NUP · Mitochondria.

New: STAR deepRED & STAR 550 expand the FluoTag® portfolio.

@nanotag.bsky.social

#SmartSecondaries #MulticolorImaging #abberior
June 15, 2026 at 2:03 PM
Anti-RFP sdABs licensed from @nanotag.bsky.social, where they're available unmodified and fluorophore-coupled (FluoTag®)
July 8, 2025 at 8:04 AM
✨ 𝗕𝗹𝗶𝗻𝗸, 𝗕𝗹𝗶𝗻𝗸! #FluorescenceFriday is perfect to launch new 🦙sdAb: 𝐅𝐥𝐮𝐨𝐓𝐚𝐠𝐬® 𝐜𝐨𝐧𝐣𝐮𝐠𝐚𝐭𝐞𝐝 𝐭𝐨 𝐉𝐚𝐧𝐞𝐥𝐢𝐚 𝐅𝐥𝐮𝐨𝐫® 𝟔𝟑𝟓𝐛 a new buffer-independent self-blinking dye. Making #SMLM more precise and reproducible! Now you find FluoTag®-X2 𝐚𝐧𝐭𝐢-𝐀𝐋𝐅𝐀, 𝐚𝐧𝐭𝐢-𝐆𝐅𝐏, and 𝐚𝐧𝐭𝐢-𝐦𝐄𝐨𝐬, more coming soon. nano-tag.com/shop/
December 13, 2024 at 10:05 AM
📄 A recent study in #ACSNano by @heilemannlab.bsky.social shows that 🔬STED microscopy with DNA-fluorophore labels enables intensity-based molecular counting. Happy to see our #nanobodies 🦙 FluoTag®-X4 anti-GFP and sdAb anti-ALFA contribute to this work.
Read the paper here: doi.org/10.1021/acsn...
April 10, 2026 at 8:12 AM
Astrocytes never fail to amaze 🫠✨
This beautiful GFAP⁺ network by Svilen Georgiev (Rizzoli Lab, UMG) was stained with our fluorescent #nanobody FluoTag®-X2 anti-GFAP, revealing an intricate, highly interconnected network of filament-rich processes 🧠
#sdAb #Microscopy #Neuroscience #Astrocytes #GFAP
May 6, 2026 at 10:30 AM
Meet #NanoTag at the #FOM2026 in Stockholm!
Visit booth 24 to explore our 🦙 #nanobody -based solutions tailored for quantitative super-resolution microscopy and flexible #multiplexing 🔬.

Our team is looking forward to connecting with you!

#SingleDomainAntibodies
#SmartSecondaries #FluoTag
March 30, 2026 at 1:00 PM
💬 “I’m obsessed with your anti-PSD95 nanobody!” — auditory neuroscientist Roos Anouk Voorn said at our booth during the #NWG2025 Meeting @neurowissg.bsky.social. Moments like this are why we do what we do. 🔬💙🧠 Discover FluoTag®-X2 anti-PSD95 👉 bit.ly/4iXYbjA #Nanobody #PSD95 #Neuroscience #WhyWeDoIt
April 2, 2025 at 10:28 AM
📸 3plex IF using two mouse primary Abs premixed with #SmartSecondaries for 15 min at RT & combined with FluoTag®-X4 anti-GFP STAR deepRED for OneStep staining:
🟠 Vimentin – FluoTag®-X2 anti-Mouse IgG1 STAR ORANGE
🔵 NPC – FluoTag®-X2 anti-Mouse IgG1 STAR RED
🔴 NUP96-GFP – FluoTag®-X4 anti-GFP deepRED
June 15, 2026 at 12:16 PM
🔬🌈 We're expanding the spectral range of #abberior STAR-conjugated #nanobodies! 🤩

STAR 550 and deepRED have joined the range.

Choose Smart Secondaries® and FluoTag®-X4 anti-GFP in your favorite colors and #multiplex with ease.

🦙🍩 In collaboration with @abberior.rocks team!
#Microscopy #STED
June 15, 2026 at 12:16 PM
We give some recommendations for on:off ratios based on sample and technique. Use HaloTag? Swap in a "b" dye (email me). Use ALFA-tag? Get the Nb with JF635b: nano-tag.com/product/fluo... Want to conjugate your own? Tocris has your back: www.tocris.com/product-type...
FluoTag®-X2 anti-ALFA - NanoTag Biotechnologies
FluoTag®-X2 anti-ALFA is a fluorescently labeled single-domain antibody (sdAb) that specifically detects the small, rationally designed ALFA-tag. Enables direct and highly specific fluorescent detecti...
nano-tag.com
April 17, 2026 at 11:49 AM
📸 Huge thanks to Roman Tsukanov @romantsukanov.bsky.social & Samrat Basak @samratb.bsky.social for the dSTORM 🕸️ images of a COS-7 cells stained with mouse anti-Tubulin antibody premixed with FluoTag®-X2 anti-Mouse IgG1 JF635b SmartSecondary®.
October 30, 2025 at 1:06 PM
sdABs licensed from
@nanotag.bsky.social, where they're available unmodified and fluorophore-coupled (FluoTag®)
July 18, 2025 at 7:26 AM
💙On #RareDiseaseDay, we highlight the power of scientific innovation in #RareDisease research. 🔬Our #FluoTag®-X2 anti-mEos contributed to a study on Severe Startle Disease, revealing that GlyRβ enables mutGlyRα1 targeting but fails to restore function. 📄 here:
doi.org/10.1523/jneu...
February 28, 2025 at 2:47 PM
A preprint using: FluoTag®-X2 anti-ALF (RRID:AB_3075981) from @nanotag.bsky.social was published.

SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #RRID #OpenScience
Cluster nanoarchitecture and structural diversity of PIEZO1 at rest and during activation in intact cells
www.biorxiv.org
July 13, 2025 at 12:00 PM
#SuperResolution just got upgraded! FluoTag-X4 anti-GFP + @abberior.rocks STAR 🔵🟢🟠🔴 = high 🎯 sdAb & #STED-optimized brightness!
✅ Small sdAb (~3 nm); Better penetration, less linkage error
✅ 2 sdAbs = 4 fluorophores/GFP
✅ Bright & photostable
✅ No 2nd Ab needed
🚀 Upgrade #GFP imaging: bit.ly/4bC6ZJ5
March 18, 2025 at 1:51 PM
Greetings from #Horizons2026 in our hometown Göttingen! 🦙🔬Our team is here showcasing our #nanobody -based technologies and services.

Come by our booth to explore how #NanoTag might support your research!

#SingleDomainAntibody #FluoTag #SmartSecondaries #ALFAsystem
@horizonsmolbio.bsky.social
September 7, 2026 at 2:23 PM
Neuroscientists - looking for a high-affinity gift?💝

Inside the postsynapse, proteins crowd the show
PSD95’s the star - but hard to know.

IgG antibodies knock, but can’t get through
Too bulky to reach the target they pursue.

But FluoTag®-X2 anti-PSD95 slips right in
Binds PSD95. Precise within.
February 13, 2026 at 11:32 AM
🔬🫧 New tools for live-cell monitoring of #SynapticVesicle dynamics based on a #nanobody targeting the Syt1 Luminal Domain are now available in the NanoTag Shop:

• FluoTag®-X2 anti-Syt1 Luminal Domain
• sdAb anti-Syt1 Luminal Domain Halo fusion

🔥More anti-Syt1 Luminal Domain reagents coming soon!
June 8, 2026 at 9:33 AM
🔬📄 You can precomplex the sdAb anti-Syt1 Luminal Domain ALFA-tag fusion with fluorescent NbALFA (FluoTag®-X2 anti-ALFA) or with gold-conjugated NbALFA. The latter approach was demonstrated in a recent paper by @forna.bsky.social et al. link.springer.com/article/10.1...
A versatile nanobody platform for live and super-resolution imaging of synaptic vesicle dynamics and plasticity in rodent and human neurons - Journal of Nanobiotechnology
Synaptic neurotransmission is a critical hallmark of brain activity and one of the first processes affected in neural diseases. Monitoring this process, particularly synaptic vesicle recycling, in liv...
link.springer.com
August 27, 2026 at 11:24 AM
All antibodies used were produced in rabbit – only possible thanks to the smart secondary nanobodies coupled to abberior STAR dyes! 🙌 They enable species-independent multiplexing with the premix & stain protocol. 👉 info.abberior.com/new-abberior...
new abberior STAR conjugated FluoTag monoclonal nanobodies
High-resolution nanobody reagents with STAR dyes for STED, confocal, multiplex imaging, antibody labeling, deep tissue staining, fluorescence microscopy.
info.abberior.com
September 12, 2025 at 12:22 PM
📸 Left panel: PFA-fixed rat brain stained with rHcAb anti-CNPase Rb IgG Fc-fusion, detected with Smart Secondary® – FluoTag®-X2 anti-Rb IgG.
Right panel: PFA-fixed rat primary neurons stained with rHcAb anti-SYT1 (7D5) Ms IgG1 Fc-fusion, detected with Smart Secondary® – FluoTag®-X2 anti-Ms IgG1.
April 17, 2026 at 9:04 AM