- any input appreciated as they continue to work on new landing sites and applications! More to come...
#ShareYourTools #MakeMoreTransgenics #AlwaysBeCloning
(6/6)
#ShareYourTools #MakeMoreTransgenics #AlwaysBeCloning
(6/6)
February 13, 2026 at 8:42 PM
- any input appreciated as they continue to work on new landing sites and applications! More to come...
#ShareYourTools #MakeMoreTransgenics #AlwaysBeCloning
(6/6)
#ShareYourTools #MakeMoreTransgenics #AlwaysBeCloning
(6/6)
We heart #zebrafish. 🐟🧬🫀❤️
(ventral view, tmem88a:EGFP, 7xTCF-Xla.Sia:NLS-mCherry, BDM-treated for live imaging; pic credit Swapnil Hingole @cudevbio.bsky.social)
#devbio #CHD #imaging #MakeMoreTransgenics
(ventral view, tmem88a:EGFP, 7xTCF-Xla.Sia:NLS-mCherry, BDM-treated for live imaging; pic credit Swapnil Hingole @cudevbio.bsky.social)
#devbio #CHD #imaging #MakeMoreTransgenics
July 21, 2026 at 5:47 PM
We heart #zebrafish. 🐟🧬🫀❤️
(ventral view, tmem88a:EGFP, 7xTCF-Xla.Sia:NLS-mCherry, BDM-treated for live imaging; pic credit Swapnil Hingole @cudevbio.bsky.social)
#devbio #CHD #imaging #MakeMoreTransgenics
(ventral view, tmem88a:EGFP, 7xTCF-Xla.Sia:NLS-mCherry, BDM-treated for live imaging; pic credit Swapnil Hingole @cudevbio.bsky.social)
#devbio #CHD #imaging #MakeMoreTransgenics
Looking so much forward to this, grateful you have us on board!
Onwards, always.
#Zebrafish #Lungfish #MakeMoreTransgenics
Onwards, always.
#Zebrafish #Lungfish #MakeMoreTransgenics
June 25, 2026 at 10:18 PM
Looking so much forward to this, grateful you have us on board!
Onwards, always.
#Zebrafish #Lungfish #MakeMoreTransgenics
Onwards, always.
#Zebrafish #Lungfish #MakeMoreTransgenics
When your #zebrafish lab hasn't used Tol2 in ~3 years...and needs a team effort to dig up the transposase mRNA template ❄️🧊. 🐽
#piGLET #MakeMoreTransgenics
#piGLET #MakeMoreTransgenics
June 16, 2026 at 6:42 PM
When your #zebrafish lab hasn't used Tol2 in ~3 years...and needs a team effort to dig up the transposase mRNA template ❄️🧊. 🐽
#piGLET #MakeMoreTransgenics
#piGLET #MakeMoreTransgenics
#FluorescenceFriday with a transgenic zebrafish labeling descendant lineages of the lateral plate mesoderm.
(Ventral view, tmem88a:EGFP, pic cred Marycruz Flores Flores @cudevbio.bsky.social). ❤️🫀🐟🧬
#devbio #cardiacdevbio #zebrafish #MakeMoreTransgenics
(Ventral view, tmem88a:EGFP, pic cred Marycruz Flores Flores @cudevbio.bsky.social). ❤️🫀🐟🧬
#devbio #cardiacdevbio #zebrafish #MakeMoreTransgenics
January 30, 2026 at 9:58 PM
#FluorescenceFriday with a transgenic zebrafish labeling descendant lineages of the lateral plate mesoderm.
(Ventral view, tmem88a:EGFP, pic cred Marycruz Flores Flores @cudevbio.bsky.social). ❤️🫀🐟🧬
#devbio #cardiacdevbio #zebrafish #MakeMoreTransgenics
(Ventral view, tmem88a:EGFP, pic cred Marycruz Flores Flores @cudevbio.bsky.social). ❤️🫀🐟🧬
#devbio #cardiacdevbio #zebrafish #MakeMoreTransgenics
November 23, 2025 at 11:18 PM
Looking forward to when we can ask such questions for #zebrafish!
#MakeMoreTransgenics #ShareYourTools
#MakeMoreTransgenics #ShareYourTools
Quick Drosophila question: I’m planning to integrate a construct on the second chromosome but would like to avoid the attP40 landing site. Any suggestion for a good alternative attP site on chromosome II? It’s for a skeletal-muscle–related project.
Thanks! 🙂
Rui
Thanks! 🙂
Rui
September 30, 2025 at 7:12 PM
Looking forward to when we can ask such questions for #zebrafish!
#MakeMoreTransgenics #ShareYourTools
#MakeMoreTransgenics #ShareYourTools
Resources that never grow old, like:
#AlwaysBeCloning #MakeMoreTransgenics #molbio #RestrictionEnzymesStillMatter 🧪🧫🧬
#AlwaysBeCloning #MakeMoreTransgenics #molbio #RestrictionEnzymesStillMatter 🧪🧫🧬
August 20, 2025 at 7:29 PM
Resources that never grow old, like:
#AlwaysBeCloning #MakeMoreTransgenics #molbio #RestrictionEnzymesStillMatter 🧪🧫🧬
#AlwaysBeCloning #MakeMoreTransgenics #molbio #RestrictionEnzymesStillMatter 🧪🧫🧬
Grateful to receive an R24 NOA from NIH/ORIP to advance phiC31-based transgenesis tools in #zebrafish. 🐟
Collab with @aburger2009.bsky.social & Breuss labs @cuanschutz.bsky.social. 🧬
...and: big THX to everyone who already helped w/ sharing #pIGLET reagents! 🐽
#ShareYourTools #MakeMoreTransgenics
Collab with @aburger2009.bsky.social & Breuss labs @cuanschutz.bsky.social. 🧬
...and: big THX to everyone who already helped w/ sharing #pIGLET reagents! 🐽
#ShareYourTools #MakeMoreTransgenics
piglet from winnie the pooh says thank you
ALT: piglet from winnie the pooh says thank you
media.tenor.com
July 16, 2025 at 7:22 PM
Grateful to receive an R24 NOA from NIH/ORIP to advance phiC31-based transgenesis tools in #zebrafish. 🐟
Collab with @aburger2009.bsky.social & Breuss labs @cuanschutz.bsky.social. 🧬
...and: big THX to everyone who already helped w/ sharing #pIGLET reagents! 🐽
#ShareYourTools #MakeMoreTransgenics
Collab with @aburger2009.bsky.social & Breuss labs @cuanschutz.bsky.social. 🧬
...and: big THX to everyone who already helped w/ sharing #pIGLET reagents! 🐽
#ShareYourTools #MakeMoreTransgenics
It's SPIM-ing time again! @mary-flores.bsky.social leading the charge to image new reporters with Amanda Garfield & Emma Harrison. @cudevbio.bsky.social #zebrafish #MakeMoreTransgenics #Imaging #devbio
July 1, 2025 at 9:20 PM
It's SPIM-ing time again! @mary-flores.bsky.social leading the charge to image new reporters with Amanda Garfield & Emma Harrison. @cudevbio.bsky.social #zebrafish #MakeMoreTransgenics #Imaging #devbio
Someone, somewhere, needs a pic of a pIGLET-based transgenic #zebrafish today.
(pic credit Seth Jacobson, p14a.ubi:gm130-tdTomato) 🐽🍅
#MakeMoreTransgenics #AlwaysBeCloning #devbio
(pic credit Seth Jacobson, p14a.ubi:gm130-tdTomato) 🐽🍅
#MakeMoreTransgenics #AlwaysBeCloning #devbio
April 24, 2025 at 1:59 PM
Someone, somewhere, needs a pic of a pIGLET-based transgenic #zebrafish today.
(pic credit Seth Jacobson, p14a.ubi:gm130-tdTomato) 🐽🍅
#MakeMoreTransgenics #AlwaysBeCloning #devbio
(pic credit Seth Jacobson, p14a.ubi:gm130-tdTomato) 🐽🍅
#MakeMoreTransgenics #AlwaysBeCloning #devbio
/ rant on:
Abbreviating cis-regulatory elements as *CRE* is a terrible abbreviation in a field that uses *Cre* recombinase as major lineage trace tool. 🐭🐟🧬
/ rant off
#devbio #MakeMoreTransgenics #Crelox #enhancers
Abbreviating cis-regulatory elements as *CRE* is a terrible abbreviation in a field that uses *Cre* recombinase as major lineage trace tool. 🐭🐟🧬
/ rant off
#devbio #MakeMoreTransgenics #Crelox #enhancers
March 17, 2025 at 11:08 PM
/ rant on:
Abbreviating cis-regulatory elements as *CRE* is a terrible abbreviation in a field that uses *Cre* recombinase as major lineage trace tool. 🐭🐟🧬
/ rant off
#devbio #MakeMoreTransgenics #Crelox #enhancers
Abbreviating cis-regulatory elements as *CRE* is a terrible abbreviation in a field that uses *Cre* recombinase as major lineage trace tool. 🐭🐟🧬
/ rant off
#devbio #MakeMoreTransgenics #Crelox #enhancers
Achievement unlocked:
this simple box flew from CU Anschutz🌎 > University of Otago🌏, after months of paperwork & coordination.
...and now, the #zebrafish pIGLET landing site lines are in New Zealand! Looking forward to the new transgenics from the NZ community.
#ShareYourTools #MakeMoreTransgenics
this simple box flew from CU Anschutz🌎 > University of Otago🌏, after months of paperwork & coordination.
...and now, the #zebrafish pIGLET landing site lines are in New Zealand! Looking forward to the new transgenics from the NZ community.
#ShareYourTools #MakeMoreTransgenics
March 4, 2025 at 8:01 PM
Achievement unlocked:
this simple box flew from CU Anschutz🌎 > University of Otago🌏, after months of paperwork & coordination.
...and now, the #zebrafish pIGLET landing site lines are in New Zealand! Looking forward to the new transgenics from the NZ community.
#ShareYourTools #MakeMoreTransgenics
this simple box flew from CU Anschutz🌎 > University of Otago🌏, after months of paperwork & coordination.
...and now, the #zebrafish pIGLET landing site lines are in New Zealand! Looking forward to the new transgenics from the NZ community.
#ShareYourTools #MakeMoreTransgenics
90+ labs got pIGLET lines & reagents now. 🌎🌍🌏
First labs report targeted germline integrations & beautiful new transgenics - we are always keen to hear your updates!
Ping us about trouble with genotyping, cloning, etc. - email or Slack @zebrafishrock.bsky.social. 🐽
#MakeMoreTransgenics #zebrafish
First labs report targeted germline integrations & beautiful new transgenics - we are always keen to hear your updates!
Ping us about trouble with genotyping, cloning, etc. - email or Slack @zebrafishrock.bsky.social. 🐽
#MakeMoreTransgenics #zebrafish
Some small wins: this week's pIGLET shipments arrived at their new homes!
Here's the one to @mydennis.bsky.social's lab - fingers crossed for your new transgenics! 🐽🧬🐟
(tube/blot credit: Marycruz Flores Flores & Amanda Garfield)
#zebrafish #MakeMoreTransgenics #ShareYourTools
Here's the one to @mydennis.bsky.social's lab - fingers crossed for your new transgenics! 🐽🧬🐟
(tube/blot credit: Marycruz Flores Flores & Amanda Garfield)
#zebrafish #MakeMoreTransgenics #ShareYourTools
February 7, 2025 at 4:37 PM
90+ labs got pIGLET lines & reagents now. 🌎🌍🌏
First labs report targeted germline integrations & beautiful new transgenics - we are always keen to hear your updates!
Ping us about trouble with genotyping, cloning, etc. - email or Slack @zebrafishrock.bsky.social. 🐽
#MakeMoreTransgenics #zebrafish
First labs report targeted germline integrations & beautiful new transgenics - we are always keen to hear your updates!
Ping us about trouble with genotyping, cloning, etc. - email or Slack @zebrafishrock.bsky.social. 🐽
#MakeMoreTransgenics #zebrafish
Some small wins: this week's pIGLET shipments arrived at their new homes!
Here's the one to @mydennis.bsky.social's lab - fingers crossed for your new transgenics! 🐽🧬🐟
(tube/blot credit: Marycruz Flores Flores & Amanda Garfield)
#zebrafish #MakeMoreTransgenics #ShareYourTools
Here's the one to @mydennis.bsky.social's lab - fingers crossed for your new transgenics! 🐽🧬🐟
(tube/blot credit: Marycruz Flores Flores & Amanda Garfield)
#zebrafish #MakeMoreTransgenics #ShareYourTools
February 7, 2025 at 12:03 AM
Some small wins: this week's pIGLET shipments arrived at their new homes!
Here's the one to @mydennis.bsky.social's lab - fingers crossed for your new transgenics! 🐽🧬🐟
(tube/blot credit: Marycruz Flores Flores & Amanda Garfield)
#zebrafish #MakeMoreTransgenics #ShareYourTools
Here's the one to @mydennis.bsky.social's lab - fingers crossed for your new transgenics! 🐽🧬🐟
(tube/blot credit: Marycruz Flores Flores & Amanda Garfield)
#zebrafish #MakeMoreTransgenics #ShareYourTools
First zoom with new collaborator and all involved trainees =
- inspired buzzing in the lab
- puts faces to names from prior emails
- motivating common goal
...but importantly
- smiles all around!
#PILife #CHD #devbio #collaborate #GoFish #MakeMoreTransgenics 🐟🧬🧫🫀
- inspired buzzing in the lab
- puts faces to names from prior emails
- motivating common goal
...but importantly
- smiles all around!
#PILife #CHD #devbio #collaborate #GoFish #MakeMoreTransgenics 🐟🧬🧫🫀
January 14, 2025 at 5:00 PM
First zoom with new collaborator and all involved trainees =
- inspired buzzing in the lab
- puts faces to names from prior emails
- motivating common goal
...but importantly
- smiles all around!
#PILife #CHD #devbio #collaborate #GoFish #MakeMoreTransgenics 🐟🧬🧫🫀
- inspired buzzing in the lab
- puts faces to names from prior emails
- motivating common goal
...but importantly
- smiles all around!
#PILife #CHD #devbio #collaborate #GoFish #MakeMoreTransgenics 🐟🧬🧫🫀
1st pIGLET lines of 2025 coming @cudevbio.bsky.social - today:
pIGLET14-based hsp70l-driven loxP transgene with tdTomato-fused reporter + exorh:EGFP marker; tested with drl:creERT2, heat-shocked/imaged at 4 dpf.
Congrats to Amanda Garfield on her first transgenic! 🐽
#zebrafish #MakeMoreTransgenics
pIGLET14-based hsp70l-driven loxP transgene with tdTomato-fused reporter + exorh:EGFP marker; tested with drl:creERT2, heat-shocked/imaged at 4 dpf.
Congrats to Amanda Garfield on her first transgenic! 🐽
#zebrafish #MakeMoreTransgenics
January 7, 2025 at 6:40 PM
1st pIGLET lines of 2025 coming @cudevbio.bsky.social - today:
pIGLET14-based hsp70l-driven loxP transgene with tdTomato-fused reporter + exorh:EGFP marker; tested with drl:creERT2, heat-shocked/imaged at 4 dpf.
Congrats to Amanda Garfield on her first transgenic! 🐽
#zebrafish #MakeMoreTransgenics
pIGLET14-based hsp70l-driven loxP transgene with tdTomato-fused reporter + exorh:EGFP marker; tested with drl:creERT2, heat-shocked/imaged at 4 dpf.
Congrats to Amanda Garfield on her first transgenic! 🐽
#zebrafish #MakeMoreTransgenics
Lots to learn and to dig into with these new drivers - congrats all!
#collaborate #MakeMoreTransgenics
#collaborate #MakeMoreTransgenics
December 6, 2024 at 4:32 PM
Lots to learn and to dig into with these new drivers - congrats all!
#collaborate #MakeMoreTransgenics
#collaborate #MakeMoreTransgenics
Lateral plate to brighten the day ❤️⚾.
(drl:EGFP; pic cred Harrison Wells)
#zebrafish #devbio #MakeMoreTransgenics
(drl:EGFP; pic cred Harrison Wells)
#zebrafish #devbio #MakeMoreTransgenics
November 21, 2024 at 8:23 PM
Lateral plate to brighten the day ❤️⚾.
(drl:EGFP; pic cred Harrison Wells)
#zebrafish #devbio #MakeMoreTransgenics
(drl:EGFP; pic cred Harrison Wells)
#zebrafish #devbio #MakeMoreTransgenics
Ours to compare (pic from 2021 but unchanged except the fall decoration):
every transgenic or mutant ever from our lab was done with needles from this beauty. First at UZH, now @cuanschutz.bsky.social.
#zebrafish #MakeMoreTransgenics
every transgenic or mutant ever from our lab was done with needles from this beauty. First at UZH, now @cuanschutz.bsky.social.
#zebrafish #MakeMoreTransgenics
November 20, 2024 at 4:07 AM
Ours to compare (pic from 2021 but unchanged except the fall decoration):
every transgenic or mutant ever from our lab was done with needles from this beauty. First at UZH, now @cuanschutz.bsky.social.
#zebrafish #MakeMoreTransgenics
every transgenic or mutant ever from our lab was done with needles from this beauty. First at UZH, now @cuanschutz.bsky.social.
#zebrafish #MakeMoreTransgenics
#FluorescenceFriday, or when the enhancer you're testing is not doing what you expect (or understand!).
-3.4meis2b:mApple (magenta) with mpeg1.1:GFP; pic cred Hannah Moran.
Happy to share the line if anyone's interested, otherwise...🪦
#zebrafish #devbio #AlwaysBeCloning #MakeMoreTransgenics
-3.4meis2b:mApple (magenta) with mpeg1.1:GFP; pic cred Hannah Moran.
Happy to share the line if anyone's interested, otherwise...🪦
#zebrafish #devbio #AlwaysBeCloning #MakeMoreTransgenics
November 15, 2024 at 10:45 PM
#FluorescenceFriday, or when the enhancer you're testing is not doing what you expect (or understand!).
-3.4meis2b:mApple (magenta) with mpeg1.1:GFP; pic cred Hannah Moran.
Happy to share the line if anyone's interested, otherwise...🪦
#zebrafish #devbio #AlwaysBeCloning #MakeMoreTransgenics
-3.4meis2b:mApple (magenta) with mpeg1.1:GFP; pic cred Hannah Moran.
Happy to share the line if anyone's interested, otherwise...🪦
#zebrafish #devbio #AlwaysBeCloning #MakeMoreTransgenics
#NPAW2023 - big congrats to our lab's Dr. Robert Lalonde on receiving the inaugural CU Anschutz Dean's Postdoctoral Research Award!
Onwards, upwards, always.
#zebrafish #devbio #evodevo #MakeMoreTransgenics
Onwards, upwards, always.
#zebrafish #devbio #evodevo #MakeMoreTransgenics
September 21, 2023 at 2:58 AM
#NPAW2023 - big congrats to our lab's Dr. Robert Lalonde on receiving the inaugural CU Anschutz Dean's Postdoctoral Research Award!
Onwards, upwards, always.
#zebrafish #devbio #evodevo #MakeMoreTransgenics
Onwards, upwards, always.
#zebrafish #devbio #evodevo #MakeMoreTransgenics