As light travels deeper into the brain, it scatters. Three-photon microscopy, the current state of the art, reduces the resulting background fluorescence and can, in principle, image much deeper than earlier techniques—but to do that, it uses average laser power limits that cause overheating and damage living tissue.

For 22 years a speck of fossil jaw from Aberdeenshire was the world's oldest winged insect. New laser microscopy suggests it probably isn't an insect at all, giving dragonflies, bees and crickets a much shorter history.

Brandeis University is looking for a Part-time Technical Support Specialist. The main purpose of this half-time (0.5 FTE) job is to expand what the Brandeis Light Microscopy Core Facility can do for i...

The multi-immersion oblique plane microscope is a flexible and versatile light-sheet microscope that allows imaging of samples ranging from cells in culture to whole organisms or cleared tissue sample...

Researchers at Rice University developed a new method that enables single-objective light sheet microscopy in standard, commercially available sample chambers.

Researchers at Rice University developed a new method that enables single-objective light sheet microscopy in standard, commercially available sample chambers.

Researchers at Rice University developed a new method that enables single-objective light sheet microscopy in standard, commercially available sample chambers.

As light travels deeper into the brain, it scatters. Three-photon microscopy, the current state of the art, reduces the resulting background fluorescence and can, in principle, image much deeper than earlier techniques—but to do that, it uses average laser power limits that cause overheating and damage living tissue.

Strobe-Enhanced Microscopy Stage. Contribute to wenzel-lab/strobe-enhanced-microscopy-stage development by creating an account on GitHub.

Researchers have pushed the depth limits of optical imaging of brain activity by creating an optical platform that is 250-fold more efficient than conventional systems of its kind.

Scanning electron microscopy (SEM) revealed the presence of distinct vesicular structures in the pericellular region of highly migratory breast cancer cell lines (MDA-MB-231, 4T1 and MCF7) (Fig. 1a–c). We termed these structures motile vesicles (MoVes). Notably, MoVes were entirely absent in the non-tumorigenic breast epithelial cell line MCF10A (Fig. 1d). Upon transformation with the HRAS oncogene, MCF10A cells (now MCF-10AT) displayed increased cell motility (0.809 μm min−1 versus 0.24 μm min−1 in MCF10A, P < 0.0001) (Extended Data Fig. 1a–c), along with MoVes biogenesis (Fig. 1e and Supplementary Video 1a). MoVes exhibited unique structural features distinct from migrasomes, including multiple budding events and filopodia-like extensions (Fig. 1f,g). We further observed that MoVes formation occurs via a multiphase process involving: (1) initiation (early and late), (2) emission and (3) a final budding phase (Fig. 1h–k). Transmission electron microscopy (TEM) and immunofluorescence (IF) images of MoVes further revealed the presence of multiple organelles including mitochondria, lysosomes, endoplasmic reticulum and Golgi apparatus (Fig. 1l,m and Extended Data Fig. 2). To visualize MoVes biogenesis dynamically and minimize fixation artefacts inherent to SEM, we performed live-cell imaging. Cancer cells stained with wheat germ agglutinin (WGA) demonstrated the emergence of MoVes at the retracting edge of...