Need to #multiplex your #nanopore direct RNA runs? We are happy to announce an upgraded version of #SeqTagger #version2 with demuxing models for #mRNA (compatible with polyadenylated #rRNA) and #tRNA. Code and models publicly available in GitHub! github.com/novoalab/Seq...

I’m thrilled to see my artwork on the #cover of #MolecularCell!
A study by @imilenkovic.bsky.social et al used epitranscriptomic rRNA fingerprinting to identify tissue-of-origin and tumor-specific signatures. @NovoaLab @crg.eu
#sciart #scicomm #science #cancerresearch

🧬We had a great time at the Genome Biology Program Retreat'25!
Big thanks to the organisers for such an inspiring and fun event 👏
Congrats to @miemonti.bsky.social for being part of the organising team and to @oguzhanbegik.bsky.social for giving a fantastic talk! 🎤✨

#GenomeBiology #CRG #NovoaLab

First things first, we updated SeqTagger to support 96 barcodes with the newest sequencing chemistry (RNA004). If you are interested in multiplexing your own DRS runs the new model is openly available here -> github.com/novoalab/Seq.... Feedback is very welcome! (2/12)

New paper from Eva Maria Novoa's lab, demonstrating how barcoded direct RNA reads can be demultiplexed on #nanopore

github.com/novoalab/Seq...

www.biorxiv.org/content/10.1...

5) PolyTailor is a new tool that can predict tail length and content without relying on the annotation of polyA sites on Nano3P-seq data. It is a user-friendly and fast, code is already available to use! github.com/novoalab/pol...

Also 404 on my end 🧐 a quick look at the lab GitHub github.com/novoalab/ suggests to me that they forgot to make the repo public, rather than this being a typo in the URL

Leveraging the tool NanoRMS2 (github.com/novoalab/nan...) we generated labels to train the first modification-aware basecaller for m6A, which we called m6ABasecaller (github.com/novoalab/m6A...).

The paper also drops a 96-barcode demultiplexing model for SQK-RNA004 (precision 0.993, recall 1.0), very useful for large DRS experiments.

Paper: Nucleic Acids Research, Diensthuber et al. 2026
Code: github.com/novoalab/ONT_basecalling_models

#Nanopore #RNAseq #Trinobia