Open science for plant breeding 🌱
Researchers successfully used OpenCRISPR-1, and AI-designed open-source editing system, in soybean.
Tools with broader access could mean faster innovation 👇
www.biorxiv.org/content/10.6...
Researchers successfully used OpenCRISPR-1, and AI-designed open-source editing system, in soybean.
Tools with broader access could mean faster innovation 👇
www.biorxiv.org/content/10.6...
September 25, 2026 at 11:57 AM
Open science for plant breeding 🌱
Researchers successfully used OpenCRISPR-1, and AI-designed open-source editing system, in soybean.
Tools with broader access could mean faster innovation 👇
www.biorxiv.org/content/10.6...
Researchers successfully used OpenCRISPR-1, and AI-designed open-source editing system, in soybean.
Tools with broader access could mean faster innovation 👇
www.biorxiv.org/content/10.6...
OpenCRISPR was even more extreme example of this, with three sequences from Streptococcus accounting for 98% of the novelty gistpreview.github.io?f6684bb2fefa...
Gist HTML Preview
gistpreview.github.io
January 18, 2025 at 6:44 AM
OpenCRISPR was even more extreme example of this, with three sequences from Streptococcus accounting for 98% of the novelty gistpreview.github.io?f6684bb2fefa...
Now that OpenCRISPR is in nature and rekindled the 'what's-a-novel-sequence' debate, I'm happy to share an app to check this, which I built for fun some time ago.
fuerstlab.shinyapps.io/SeqNovelty/
quick 🧵
fuerstlab.shinyapps.io/SeqNovelty/
quick 🧵
August 1, 2025 at 11:50 AM
Now that OpenCRISPR is in nature and rekindled the 'what's-a-novel-sequence' debate, I'm happy to share an app to check this, which I built for fun some time ago.
fuerstlab.shinyapps.io/SeqNovelty/
quick 🧵
fuerstlab.shinyapps.io/SeqNovelty/
quick 🧵
✨ Paper spotlight ✨
(🧵 1/7) AI-designed OpenCRISPR-1 performs robust knock-out, base editing and prime editing in rice
nph.onlinelibrary.wiley.com/doi/10.1111/...
(🧵 1/7) AI-designed OpenCRISPR-1 performs robust knock-out, base editing and prime editing in rice
nph.onlinelibrary.wiley.com/doi/10.1111/...
May 22, 2026 at 2:35 PM
✨ Paper spotlight ✨
(🧵 1/7) AI-designed OpenCRISPR-1 performs robust knock-out, base editing and prime editing in rice
nph.onlinelibrary.wiley.com/doi/10.1111/...
(🧵 1/7) AI-designed OpenCRISPR-1 performs robust knock-out, base editing and prime editing in rice
nph.onlinelibrary.wiley.com/doi/10.1111/...
AI-designed OpenCRISPR-1 offers enhanced genome editing, outperforming Cas9 with h… PMID:42192532, Genome Med 2026, @GenomeMedicine @OTSociety @NAR_Open https://doi.org/10.1186/s13073-026-01682-2 #Medsky #Pharmsky #RNA #ASHG #ESHG 🧪
High-fidelity genome and prime editing enabled by the AI-designed openCRISPR-1 | Genome Medicine | Springer Nature Link
RNA-guided nucleases such as CRISPR-Cas9 systems have revolutionized genome engineering by enabling programmable DNA modifications. Although structure-guid
doi.org
May 31, 2026 at 5:10 AM
Okay so I definitely need a CRISPR system and the only real path forward is the OpenCrispr protein. A friend donated a human optimized plasmid, so first is retooling for plants. If anyone wants access to it early, help me pay for the synthesis. DM's open. 💚
a computer generated image of a cell with the word cas9 on the bottom
ALT: a computer generated image of a cell with the word cas9 on the bottom
media.tenor.com
September 27, 2025 at 6:29 PM
Okay so I definitely need a CRISPR system and the only real path forward is the OpenCrispr protein. A friend donated a human optimized plasmid, so first is retooling for plants. If anyone wants access to it early, help me pay for the synthesis. DM's open. 💚
Stole a few mins to prep these cultures for miniprep tomorrow. Another attempt at the vibrio tfoX expression circuit, an OpenCRISPR cas9 expression plasmid, and pOpenTaq Taq Polymerase expression plasmid. Gonna offer latter two in my shop :)
June 27, 2025 at 6:40 AM
Stole a few mins to prep these cultures for miniprep tomorrow. Another attempt at the vibrio tfoX expression circuit, an OpenCRISPR cas9 expression plasmid, and pOpenTaq Taq Polymerase expression plasmid. Gonna offer latter two in my shop :)
Vietnam Academy of Science and Technology researchers successfully deployed the AI-designed OpenCRISPR-1 nuclease in dicot plants, achieving 49.55% editing in soybean hairy roots and 75% T0 editing efficiency in tobacco. 🌿🤖 #OpenCRISPR #PlantGenomics #SoybeanEditing #CRISPR #bioRxiv2026 #AgBiotech
September 1, 2026 at 8:49 AM
Vietnam Academy of Science and Technology researchers successfully deployed the AI-designed OpenCRISPR-1 nuclease in dicot plants, achieving 49.55% editing in soybean hairy roots and 75% T0 editing efficiency in tobacco. 🌿🤖 #OpenCRISPR #PlantGenomics #SoybeanEditing #CRISPR #bioRxiv2026 #AgBiotech
AI-designed OpenCRISPR-1 performs robust knockout, base editing, and prime editing in rice
A #Letter by Molla et al.
👇
📖 nph.onlinelibrary.wiley.com/doi/10.1111/...
#LatestIssue #PlantScience
A #Letter by Molla et al.
👇
📖 nph.onlinelibrary.wiley.com/doi/10.1111/...
#LatestIssue #PlantScience
August 6, 2026 at 10:17 PM
AI-designed OpenCRISPR-1 performs robust knockout, base editing, and prime editing in rice
A #Letter by Molla et al.
👇
📖 nph.onlinelibrary.wiley.com/doi/10.1111/...
#LatestIssue #PlantScience
A #Letter by Molla et al.
👇
📖 nph.onlinelibrary.wiley.com/doi/10.1111/...
#LatestIssue #PlantScience
*Ahem*: plant molecular farmer here…just about everything we express in plants is human codon-optimised, and preferably up to 60% G+C - because it just WORKS! So a human-optimised OpenCrispr should be fine in N benth!!
September 27, 2025 at 7:15 PM
*Ahem*: plant molecular farmer here…just about everything we express in plants is human codon-optimised, and preferably up to 60% G+C - because it just WORKS! So a human-optimised OpenCrispr should be fine in N benth!!
Have folks had the chance to try out the free-to-use OpenCRISPR yet?
It seems to offer a great alternative for cash-strapped startups.
www.nature.com/articles/s41...
It seems to offer a great alternative for cash-strapped startups.
www.nature.com/articles/s41...
Design of highly functional genome editors by modelling CRISPR–Cas sequences - Nature
Gene editors designed using artificial intelligence can undertake precision editing of the human genome.
www.nature.com
September 30, 2025 at 10:29 PM
Have folks had the chance to try out the free-to-use OpenCRISPR yet?
It seems to offer a great alternative for cash-strapped startups.
www.nature.com/articles/s41...
It seems to offer a great alternative for cash-strapped startups.
www.nature.com/articles/s41...
"Alignment to NCBI-nr showed top hits to proteins from several Streptococcus spp. (Streptococcus cristatus, S. pyogenes and Streptococcus sanguinis), but none exceeding a sequence identity of 86.3%. Taken together, these three natural Cas9s yielded a cumulative identity of 98.3% for OpenCRISPR-1"
July 31, 2025 at 7:35 PM
"Alignment to NCBI-nr showed top hits to proteins from several Streptococcus spp. (Streptococcus cristatus, S. pyogenes and Streptococcus sanguinis), but none exceeding a sequence identity of 86.3%. Taken together, these three natural Cas9s yielded a cumulative identity of 98.3% for OpenCRISPR-1"
😱
The most extensive dataset of #CRISPR operons curated to-date (1,246,163)
Trained large language models (LLMs) of the dataset (ProGen2-base language model)
OpenCRISPR-1, a highly performant AI-generated gene editor that exhibits compatibility with base editing
www.biorxiv.org/content/10.1...
The most extensive dataset of #CRISPR operons curated to-date (1,246,163)
Trained large language models (LLMs) of the dataset (ProGen2-base language model)
OpenCRISPR-1, a highly performant AI-generated gene editor that exhibits compatibility with base editing
www.biorxiv.org/content/10.1...
Design of highly functional genome editors by modeling the universe of CRISPR-Cas sequences
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
www.biorxiv.org
April 23, 2024 at 9:28 AM
😱
The most extensive dataset of #CRISPR operons curated to-date (1,246,163)
Trained large language models (LLMs) of the dataset (ProGen2-base language model)
OpenCRISPR-1, a highly performant AI-generated gene editor that exhibits compatibility with base editing
www.biorxiv.org/content/10.1...
The most extensive dataset of #CRISPR operons curated to-date (1,246,163)
Trained large language models (LLMs) of the dataset (ProGen2-base language model)
OpenCRISPR-1, a highly performant AI-generated gene editor that exhibits compatibility with base editing
www.biorxiv.org/content/10.1...
I admit I only skimmed the Profluent OpenCRISPR paper, but I wanted to see how unique the Gen AI sequence composition is. Surprisingly to me, I can recreate 98% of the sequence (all but 24 amino acids) with 3 Streptococcus sequences. gistpreview.github.io?f6684bb2fefa...
April 25, 2024 at 7:52 PM
I admit I only skimmed the Profluent OpenCRISPR paper, but I wanted to see how unique the Gen AI sequence composition is. Surprisingly to me, I can recreate 98% of the sequence (all but 24 amino acids) with 3 Streptococcus sequences. gistpreview.github.io?f6684bb2fefa...
Plant Science Research Weekly -- Letter. OpenCRISPR-1 in rice: when AI meets plant genome editing (New Phytologist) (Summary by Ching Chan @ntnuchanlab.bsky.social) buff.ly/LojGmez
#PlantaePSRW
#PlantaePSRW
Letter. OpenCRISPR-1 in rice: when AI meets plant genome editing | Plantae
Genome editing has rapidly transformed biology, and Cas9 remains the most widely used tool for making targeted changes in DNA. Recently, a new AI-designed editor called OpenCRISPR-1 (OC1) which…
buff.ly
June 12, 2026 at 10:15 AM
Plant Science Research Weekly -- Letter. OpenCRISPR-1 in rice: when AI meets plant genome editing (New Phytologist) (Summary by Ching Chan @ntnuchanlab.bsky.social) buff.ly/LojGmez
#PlantaePSRW
#PlantaePSRW
We note the 86% identity to S. cristatus Cas9 in the paper, but importantly found that OpenCRISPR-1 was <80% identity to anything in our database of patented sequences.
April 26, 2024 at 1:28 AM
We note the 86% identity to S. cristatus Cas9 in the paper, but importantly found that OpenCRISPR-1 was <80% identity to anything in our database of patented sequences.
One edge case I like is openCRISPR which IIRC had a sequence that was almost entirely mixed and matched from 2 or 3 extant sequences. But since it was not done deliberately (ProGen designed it), I still think it qualifies as de novo design
January 5, 2025 at 6:33 PM
One edge case I like is openCRISPR which IIRC had a sequence that was almost entirely mixed and matched from 2 or 3 extant sequences. But since it was not done deliberately (ProGen designed it), I still think it qualifies as de novo design
RRIDs were included in this in aBIOTECH paper. Thanks for making your methods matter! #OpenScience #accelerateopenscience #methodsmatter
AI-Designed OpenCRISPR-1 Enables Efficient Targeted Mutagenesis and Prime Editing in Rice
Read the full paper: AI-Designed OpenCRISPR-1 Enables Efficient Targeted Mutagenesis and Prime Editing in Rice
doi.org
May 29, 2026 at 7:01 AM
RRIDs were included in this in aBIOTECH paper. Thanks for making your methods matter! #OpenScience #accelerateopenscience #methodsmatter
The authors included RRIDs in their in Genome Medicine paper! Thanks for making your methods matter! #ReproducibleResearch #OpenResearch #reproducibility
High-fidelity genome and prime editing enabled by the AI-designed openCRISPR-1
Read the full paper: High-fidelity genome and prime editing enabled by the AI-designed openCRISPR-1
doi.org
August 10, 2026 at 7:00 AM
The authors included RRIDs in their in Genome Medicine paper! Thanks for making your methods matter! #ReproducibleResearch #OpenResearch #reproducibility
What if 𝗔𝗜 could design 𝗯𝗲𝘁𝘁𝗲𝗿 𝗴𝗲𝗻𝗲 𝗲𝗱𝗶𝘁𝗼𝗿𝘀 than what evolution gave us? 𝗢𝗽𝗲𝗻𝗖𝗥𝗜𝗦𝗣𝗥-𝟭 - the first AI-designed genome editing enzyme - might be exactly that.
In our 𝗹𝗮𝘁𝗲𝘀𝘁 𝗯𝗹𝗼𝗴, we explain:
✔What OpenCRISPR-1 is & how it was created
✔Why developers should care
👉Read the 𝗯𝗹𝗼𝗴: hubs.li/Q03YjfQp0
In our 𝗹𝗮𝘁𝗲𝘀𝘁 𝗯𝗹𝗼𝗴, we explain:
✔What OpenCRISPR-1 is & how it was created
✔Why developers should care
👉Read the 𝗯𝗹𝗼𝗴: hubs.li/Q03YjfQp0
December 17, 2025 at 10:50 AM
What if 𝗔𝗜 could design 𝗯𝗲𝘁𝘁𝗲𝗿 𝗴𝗲𝗻𝗲 𝗲𝗱𝗶𝘁𝗼𝗿𝘀 than what evolution gave us? 𝗢𝗽𝗲𝗻𝗖𝗥𝗜𝗦𝗣𝗥-𝟭 - the first AI-designed genome editing enzyme - might be exactly that.
In our 𝗹𝗮𝘁𝗲𝘀𝘁 𝗯𝗹𝗼𝗴, we explain:
✔What OpenCRISPR-1 is & how it was created
✔Why developers should care
👉Read the 𝗯𝗹𝗼𝗴: hubs.li/Q03YjfQp0
In our 𝗹𝗮𝘁𝗲𝘀𝘁 𝗯𝗹𝗼𝗴, we explain:
✔What OpenCRISPR-1 is & how it was created
✔Why developers should care
👉Read the 𝗯𝗹𝗼𝗴: hubs.li/Q03YjfQp0
Completely agree. My theory is that mean-pooling performance is overstated bc these benchmark sets comprise of sequences w same length; signal from important residues gets equally diluted by mean-pooling. My guess is mean-pooling performs worse in variable-length cases such as the OpenCRISPR library
November 26, 2024 at 12:33 PM
Completely agree. My theory is that mean-pooling performance is overstated bc these benchmark sets comprise of sequences w same length; signal from important residues gets equally diluted by mean-pooling. My guess is mean-pooling performs worse in variable-length cases such as the OpenCRISPR library
OpenCRISPR-1, Profluent's AI-designed gene editor, is now published in Nature!
@thisismadani.bsky.social emphasizes the need to move away from random discovery and into bespoke design using AI. 1/
Read more at GEN:
www.genengnews.com/topics/artif...
@thisismadani.bsky.social emphasizes the need to move away from random discovery and into bespoke design using AI. 1/
Read more at GEN:
www.genengnews.com/topics/artif...
Profluent’s AI-Designed Gene Editor Provides Glimpse Into Generalizable Platform
OpenCRISPR-1, an AI-generated custom gene editor capable of precision editing of the human genome. is just the beginning of Profluent’s AI applications.
www.genengnews.com
July 30, 2025 at 3:51 PM
OpenCRISPR-1, Profluent's AI-designed gene editor, is now published in Nature!
@thisismadani.bsky.social emphasizes the need to move away from random discovery and into bespoke design using AI. 1/
Read more at GEN:
www.genengnews.com/topics/artif...
@thisismadani.bsky.social emphasizes the need to move away from random discovery and into bespoke design using AI. 1/
Read more at GEN:
www.genengnews.com/topics/artif...
This study validates rice-tailored AI-designed open-source OpenCRISPR-1 matching SpCas9 in editing efficiency, generating blight-resistant lines, compatible with OpsgRNA and high-fidelity OpenPE6c as an open precision crop breeding platform. doi.org/10.1016/j.ab...
July 5, 2026 at 10:47 AM
This study validates rice-tailored AI-designed open-source OpenCRISPR-1 matching SpCas9 in editing efficiency, generating blight-resistant lines, compatible with OpsgRNA and high-fidelity OpenPE6c as an open precision crop breeding platform. doi.org/10.1016/j.ab...
Hey, happy to provide more info here! We did find other generated sequences with activity further from naturals (including S. cristatus), but for OpenCRISPR-1 we wanted a sequence that’d be viable as a drop-in for SpCas9 (high activity, NGG PAM), which ended up bringing us closer to a few naturals.
April 26, 2024 at 1:27 AM
Hey, happy to provide more info here! We did find other generated sequences with activity further from naturals (including S. cristatus), but for OpenCRISPR-1 we wanted a sequence that’d be viable as a drop-in for SpCas9 (high activity, NGG PAM), which ended up bringing us closer to a few naturals.
Beijing Institute of Technology researchers synthesized CRISPR engineering into a DBTL framework, showing how structural tuning, DMS, and generative AI (OpenCRISPR-1) are overcoming PAM and size bottlenecks. 🧬🤖 #CRISPR #GeneEditing #ACSSynBio2026 #GenerativeAI #SyntheticBiology #AgBiotech
August 19, 2026 at 8:03 AM
Beijing Institute of Technology researchers synthesized CRISPR engineering into a DBTL framework, showing how structural tuning, DMS, and generative AI (OpenCRISPR-1) are overcoming PAM and size bottlenecks. 🧬🤖 #CRISPR #GeneEditing #ACSSynBio2026 #GenerativeAI #SyntheticBiology #AgBiotech