Our latest preprint! - led by @makris1.bsky.social
we find that Podoplanin expression in lymphoid tissue fibroblasts regulated their inflammatory signalling and crosstalk with immune cell populations
doi.org/10.1101/2022...
we find that Podoplanin expression in lymphoid tissue fibroblasts regulated their inflammatory signalling and crosstalk with immune cell populations
doi.org/10.1101/2022...
November 22, 2024 at 3:33 PM
Our latest preprint! - led by @makris1.bsky.social
we find that Podoplanin expression in lymphoid tissue fibroblasts regulated their inflammatory signalling and crosstalk with immune cell populations
doi.org/10.1101/2022...
we find that Podoplanin expression in lymphoid tissue fibroblasts regulated their inflammatory signalling and crosstalk with immune cell populations
doi.org/10.1101/2022...
Our latest paper is finally out! We are showing the significance of the podoplanin/CLEC-2 signaling axis beyond controlling cellular mechanics and actomyosin contractility.
#fibroblasts #podoplanin #stroma #inflammation
@actonlab.bsky.social
www.cell.com/cell-reports...
#fibroblasts #podoplanin #stroma #inflammation
@actonlab.bsky.social
www.cell.com/cell-reports...
Lymph node fibroblast phenotypes and immune crosstalk regulated by podoplanin activity
Makris et al. show that podoplanin regulates the transcription profile of lymph node
fibroblasts. Podoplanin deletion drives inflammation, disrupts lymph node architecture,
and attenuates stromal/immu...
www.cell.com
June 29, 2025 at 3:51 PM
Our latest paper is finally out! We are showing the significance of the podoplanin/CLEC-2 signaling axis beyond controlling cellular mechanics and actomyosin contractility.
#fibroblasts #podoplanin #stroma #inflammation
@actonlab.bsky.social
www.cell.com/cell-reports...
#fibroblasts #podoplanin #stroma #inflammation
@actonlab.bsky.social
www.cell.com/cell-reports...
Pleased to update this - The paper is published in Cell Reports!
A long road for this one, the paper grew exponentially and evolved over a couple of years. Thanks to @makris1.bsky.social for sticking with it to untangle all the new data!
@cp-cellreports.bsky.social
www.cell.com/cell-reports...
A long road for this one, the paper grew exponentially and evolved over a couple of years. Thanks to @makris1.bsky.social for sticking with it to untangle all the new data!
@cp-cellreports.bsky.social
www.cell.com/cell-reports...
September 11, 2025 at 3:48 PM
Pleased to update this - The paper is published in Cell Reports!
A long road for this one, the paper grew exponentially and evolved over a couple of years. Thanks to @makris1.bsky.social for sticking with it to untangle all the new data!
@cp-cellreports.bsky.social
www.cell.com/cell-reports...
A long road for this one, the paper grew exponentially and evolved over a couple of years. Thanks to @makris1.bsky.social for sticking with it to untangle all the new data!
@cp-cellreports.bsky.social
www.cell.com/cell-reports...
NEW @preprintclub.bsky.social Journal Club of @biorxiv-immuno.bsky.social paper by @makris1.bsky.social @actonlab.bsky.social showing that loss of podoplanin in fibroblastic reticular cells results in decreased antibody levels and smaller germinal centres after immunization
Podoplanin mediates stromal–immune crosstalk in the lymph node - Nature Reviews Immunology
A preprint by Makris et al. shows that podoplanin is a key regulator of the functions of fibroblastic reticular cells in lymph nodes, by modulating interactions with immune cells.
www.nature.com
February 6, 2025 at 1:32 PM
NEW @preprintclub.bsky.social Journal Club of @biorxiv-immuno.bsky.social paper by @makris1.bsky.social @actonlab.bsky.social showing that loss of podoplanin in fibroblastic reticular cells results in decreased antibody levels and smaller germinal centres after immunization
Lymph node fibroblast phenotypes and immune crosstalk regulated by podoplanin activity
www.cell.com/cell-reports...
@actonlab.bsky.social @cp-cellreports.bsky.social
www.cell.com/cell-reports...
@actonlab.bsky.social @cp-cellreports.bsky.social
June 29, 2025 at 2:44 AM
Lymph node fibroblast phenotypes and immune crosstalk regulated by podoplanin activity
www.cell.com/cell-reports...
@actonlab.bsky.social @cp-cellreports.bsky.social
www.cell.com/cell-reports...
@actonlab.bsky.social @cp-cellreports.bsky.social
And Erika McCartney from Toronto will present a preprint about the effect of Podoplanin expression on the phenotypes and immunoregulatory properties of fibroblasts in lymph nodes. www.biorxiv.org/content/10.1...
Lymph Node Fibroblast Phenotypes and Immune Crosstalk Regulated by Podoplanin Activity
Lymph nodes are uniquely organised niches for immune interactions. Fibroblastic reticular cells (FRCs) facilitate immune cell communication and regulate immune function by producing growth factors, ch...
www.biorxiv.org
December 18, 2024 at 2:48 PM
And Erika McCartney from Toronto will present a preprint about the effect of Podoplanin expression on the phenotypes and immunoregulatory properties of fibroblasts in lymph nodes. www.biorxiv.org/content/10.1...
This #FluorescenceFriday we are shining a light on lymphatics in skin using Ankyron® clone DE40213, which binds Podoplanin (cyan). Image captured with our @discoverecho.bsky.social #microscope. Learn more about our recombinant antibody-replacements at proimmune.com/ankyron
December 5, 2025 at 11:11 AM
This #FluorescenceFriday we are shining a light on lymphatics in skin using Ankyron® clone DE40213, which binds Podoplanin (cyan). Image captured with our @discoverecho.bsky.social #microscope. Learn more about our recombinant antibody-replacements at proimmune.com/ankyron
PDPN marks lymphatic vessels & CAFs in the TME, but tumor-cell PDPN can also be a biomarker of a plastic lymphatic metastasis–permissive state in TNBC🔬(key fatures of the PDPN state mouse–human conserved). Hypoxia → PDPN⁺ tumour cells → CCR7⁺ lymphatic spread.
BJC: www.nature.com/articles/s41...
BJC: www.nature.com/articles/s41...
Podoplanin-defined tumour plasticity and CCR7-mediated lymphatic metastasis in triple-negative breast cancer - British Journal of Cancer
British Journal of Cancer - Podoplanin-defined tumour plasticity and CCR7-mediated lymphatic metastasis in triple-negative breast cancer
www.nature.com
April 29, 2026 at 12:45 PM
PDPN marks lymphatic vessels & CAFs in the TME, but tumor-cell PDPN can also be a biomarker of a plastic lymphatic metastasis–permissive state in TNBC🔬(key fatures of the PDPN state mouse–human conserved). Hypoxia → PDPN⁺ tumour cells → CCR7⁺ lymphatic spread.
BJC: www.nature.com/articles/s41...
BJC: www.nature.com/articles/s41...
Dr. Sophie Acton (@actonlab.bsky.social/UCL)
is a Cancer Research UK Senior Fellow with an interest in lymphoid tissue biology.
At #KSStromal26, she'll be sharing her insights on podoplanin - a molecule that controls DC motility within the lymph node.
Register now! Link in bio.
is a Cancer Research UK Senior Fellow with an interest in lymphoid tissue biology.
At #KSStromal26, she'll be sharing her insights on podoplanin - a molecule that controls DC motility within the lymph node.
Register now! Link in bio.
September 24, 2025 at 6:37 AM
Dr. Sophie Acton (@actonlab.bsky.social/UCL)
is a Cancer Research UK Senior Fellow with an interest in lymphoid tissue biology.
At #KSStromal26, she'll be sharing her insights on podoplanin - a molecule that controls DC motility within the lymph node.
Register now! Link in bio.
is a Cancer Research UK Senior Fellow with an interest in lymphoid tissue biology.
At #KSStromal26, she'll be sharing her insights on podoplanin - a molecule that controls DC motility within the lymph node.
Register now! Link in bio.
JEM’s January issue is here! rupress.org/jem/issue/22...
The cover is a light-sheet #microscopy image of a cleared, immunostained human skin biopsy depicting podoplanin-positive, αSMA-negative lymphatic vessels (yellow-red) & αSMA-positive blood vessels (blue). (Bauer et al. doi.org/10.1084/jem....)
The cover is a light-sheet #microscopy image of a cleared, immunostained human skin biopsy depicting podoplanin-positive, αSMA-negative lymphatic vessels (yellow-red) & αSMA-positive blood vessels (blue). (Bauer et al. doi.org/10.1084/jem....)
January 5, 2026 at 4:30 PM
JEM’s January issue is here! rupress.org/jem/issue/22...
The cover is a light-sheet #microscopy image of a cleared, immunostained human skin biopsy depicting podoplanin-positive, αSMA-negative lymphatic vessels (yellow-red) & αSMA-positive blood vessels (blue). (Bauer et al. doi.org/10.1084/jem....)
The cover is a light-sheet #microscopy image of a cleared, immunostained human skin biopsy depicting podoplanin-positive, αSMA-negative lymphatic vessels (yellow-red) & αSMA-positive blood vessels (blue). (Bauer et al. doi.org/10.1084/jem....)
The perinecrotic niche of glioblastoma drives
tumor-associated macrophage polarization and
immunosuppression via podoplanin-mediated CLEC5A
activation: doi.org/10.1172/JCI1...
tumor-associated macrophage polarization and
immunosuppression via podoplanin-mediated CLEC5A
activation: doi.org/10.1172/JCI1...
July 25, 2026 at 12:00 PM
The perinecrotic niche of glioblastoma drives
tumor-associated macrophage polarization and
immunosuppression via podoplanin-mediated CLEC5A
activation: doi.org/10.1172/JCI1...
tumor-associated macrophage polarization and
immunosuppression via podoplanin-mediated CLEC5A
activation: doi.org/10.1172/JCI1...
The perinecrotic niche of glioblastoma drives
tumor-associated macrophage polarization and
immunosuppression via podoplanin-mediated CLEC5A
activation: doi.org/10.1172/JCI1...
tumor-associated macrophage polarization and
immunosuppression via podoplanin-mediated CLEC5A
activation: doi.org/10.1172/JCI1...
July 20, 2026 at 12:03 PM
The perinecrotic niche of glioblastoma drives
tumor-associated macrophage polarization and
immunosuppression via podoplanin-mediated CLEC5A
activation: doi.org/10.1172/JCI1...
tumor-associated macrophage polarization and
immunosuppression via podoplanin-mediated CLEC5A
activation: doi.org/10.1172/JCI1...
This study shows that MMP13 promotes the formation of #DeepVeinThrombosis (DVT) while suppressing #Podoplanin expression, thereby advancing our understanding of #Thrombosis biology & offering new insights for DVT intervention. #medsky
Read: link.springer.com/article/10.1...
Read: link.springer.com/article/10.1...
January 21, 2026 at 3:00 AM
This study shows that MMP13 promotes the formation of #DeepVeinThrombosis (DVT) while suppressing #Podoplanin expression, thereby advancing our understanding of #Thrombosis biology & offering new insights for DVT intervention. #medsky
Read: link.springer.com/article/10.1...
Read: link.springer.com/article/10.1...
Podoplanin-Linked Mesenchymal Shift Synergizes with CCR7 driving Lymphatic Metastasis and Tumor Progression in Breast Cancer https://www.biorxiv.org/content/10.1101/2025.03.17.643704v1
March 18, 2025 at 5:50 AM
Podoplanin-Linked Mesenchymal Shift Synergizes with CCR7 driving Lymphatic Metastasis and Tumor Progression in Breast Cancer https://www.biorxiv.org/content/10.1101/2025.03.17.643704v1
Podoplanin-positive cell-derived small extracellular vesicles contribute to cardiac amyloidosis after myocardial infarction
Podoplanin-positive cell-derived small extracellular vesicles contribute to cardiac amyloidosis after myocardial infarction
Cumbersome serum amyloid A 3 (SAA3) structures accumulate in the heart after myocardial infarction, impairing left ventricle wall contractility. Cimini et al. report that exosomal SAA3 triggers SAA3 overproduction in macrophages after binding Toll-like…
dlvr.it
March 11, 2025 at 11:24 PM
Podoplanin-positive cell-derived small extracellular vesicles contribute to cardiac amyloidosis after myocardial infarction
Understanding the Role of Podoplanin in Glioblastoma Progression
www.thailandmedical.news/news/underst...
www.thailandmedical.news/news/underst...
Understanding the Role of Podoplanin in Glioblastoma Progression - Thailand Medical News
www.thailandmedical.news
December 5, 2024 at 8:19 AM
Understanding the Role of Podoplanin in Glioblastoma Progression
www.thailandmedical.news/news/underst...
www.thailandmedical.news/news/underst...
Lymph node fibroblast phenotypes and immune crosstalk regulated by podoplanin activity
Lymph node fibroblast phenotypes and immune crosstalk regulated by podoplanin activity
Makris et al. show that podoplanin regulates the transcription profile of lymph node fibroblasts. Podoplanin deletion drives inflammation, disrupts lymph node architecture, and attenuates stromal/immune cell crosstalk. This study shows the significance of the podoplanin/CLEC-2 signaling axis beyond controlling cellular mechanics and actomyosin contractility.
dlvr.it
July 7, 2025 at 7:50 PM
Lymph node fibroblast phenotypes and immune crosstalk regulated by podoplanin activity
🔬 From our March issue: rupress.org/jem/issue/22...
Confocal laser microscopy image of ileal GALT labeled for CCL19 RNA (green) & podoplanin (red). From a study "Fibroblast diversity within human gut-associated lymphoid tissues," by @ursmoerbe.bsky.social and colleagues: rupress.org/jem/article/...
Confocal laser microscopy image of ileal GALT labeled for CCL19 RNA (green) & podoplanin (red). From a study "Fibroblast diversity within human gut-associated lymphoid tissues," by @ursmoerbe.bsky.social and colleagues: rupress.org/jem/article/...
March 19, 2026 at 2:03 PM
🔬 From our March issue: rupress.org/jem/issue/22...
Confocal laser microscopy image of ileal GALT labeled for CCL19 RNA (green) & podoplanin (red). From a study "Fibroblast diversity within human gut-associated lymphoid tissues," by @ursmoerbe.bsky.social and colleagues: rupress.org/jem/article/...
Confocal laser microscopy image of ileal GALT labeled for CCL19 RNA (green) & podoplanin (red). From a study "Fibroblast diversity within human gut-associated lymphoid tissues," by @ursmoerbe.bsky.social and colleagues: rupress.org/jem/article/...
Very happy to be a part of this beautiful study led by Drs Maria Cimini and Raj Kishore showing a role for SAA3 amyloidosis after myocardial infarction (MI) and how SAA3 loaded exosomes can drive injury
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Podoplanin-positive cell-derived small extracellular vesicles contribute to cardiac amyloidosis after myocardial infarction
Cardiac amyloidosis is a secondary phenomenon of an already pre-existing chronic condition. Whether cardiac amyloidosis represents one of the complica…
www.sciencedirect.com
March 13, 2025 at 3:58 PM
Very happy to be a part of this beautiful study led by Drs Maria Cimini and Raj Kishore showing a role for SAA3 amyloidosis after myocardial infarction (MI) and how SAA3 loaded exosomes can drive injury
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
@jem.org's January issue is here! rupress.org/jem/issue/22...
The cover is a light-sheet #microscopy image of a cleared, immunostained human skin biopsy depicting podoplanin-positive, αSMA-negative lymphatic vessels & αSMA-positive blood vessels. (Bauer et al. doi.org/10.1084/jem....)
The cover is a light-sheet #microscopy image of a cleared, immunostained human skin biopsy depicting podoplanin-positive, αSMA-negative lymphatic vessels & αSMA-positive blood vessels. (Bauer et al. doi.org/10.1084/jem....)
January 5, 2026 at 4:45 PM
@jem.org's January issue is here! rupress.org/jem/issue/22...
The cover is a light-sheet #microscopy image of a cleared, immunostained human skin biopsy depicting podoplanin-positive, αSMA-negative lymphatic vessels & αSMA-positive blood vessels. (Bauer et al. doi.org/10.1084/jem....)
The cover is a light-sheet #microscopy image of a cleared, immunostained human skin biopsy depicting podoplanin-positive, αSMA-negative lymphatic vessels & αSMA-positive blood vessels. (Bauer et al. doi.org/10.1084/jem....)
Original preprint here:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Lymph Node Fibroblast Phenotypes and Immune Crosstalk Regulated by Podoplanin Activity
Lymph nodes are uniquely organised niches for immune interactions. Fibroblastic reticular cells (FRCs) facilitate immune cell communication and regulate immune function by producing growth factors, ch...
www.biorxiv.org
February 6, 2025 at 1:32 PM
Original preprint here:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Mesenteric adipose tissue imaging (2)
Adipo-Clear removes lipids while preserving tissue structure, allow high-resolution fluorescent imaging of adipose tissue. Subsequent immunolabeling uses anti-human podoplanin antibody and anti-sheep secondary antibody conjugated with Alexa 647.
Adipo-Clear removes lipids while preserving tissue structure, allow high-resolution fluorescent imaging of adipose tissue. Subsequent immunolabeling uses anti-human podoplanin antibody and anti-sheep secondary antibody conjugated with Alexa 647.
April 28, 2026 at 11:44 AM
Mesenteric adipose tissue imaging (2)
Adipo-Clear removes lipids while preserving tissue structure, allow high-resolution fluorescent imaging of adipose tissue. Subsequent immunolabeling uses anti-human podoplanin antibody and anti-sheep secondary antibody conjugated with Alexa 647.
Adipo-Clear removes lipids while preserving tissue structure, allow high-resolution fluorescent imaging of adipose tissue. Subsequent immunolabeling uses anti-human podoplanin antibody and anti-sheep secondary antibody conjugated with Alexa 647.
New preprint! Katerina Morgaenko showed that #ExVivo lymph node tissue supports cancer cell invasion. Ex vivo = mix/match cancer cells and tissue to study chemotactic mechanisms without lymphatic entry barriers. #CancerImmunology #ImmunoEngineering
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
July 23, 2024 at 2:08 AM
New preprint! Katerina Morgaenko showed that #ExVivo lymph node tissue supports cancer cell invasion. Ex vivo = mix/match cancer cells and tissue to study chemotactic mechanisms without lymphatic entry barriers. #CancerImmunology #ImmunoEngineering
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
🐭 Just published online in JPS❣️
"Podoplanin hetero-insufficiency mice with inflammation in the jejunum demonstrates a good animal model of congenital protein-losing enteropathy"
-- Toshio Ohhashi et al.
🔗 [https://doi.org/10.1016/j.jphyss.2025.100031]
#MouseModel #JPhysiolSci
"Podoplanin hetero-insufficiency mice with inflammation in the jejunum demonstrates a good animal model of congenital protein-losing enteropathy"
-- Toshio Ohhashi et al.
🔗 [https://doi.org/10.1016/j.jphyss.2025.100031]
#MouseModel #JPhysiolSci
July 23, 2025 at 12:31 AM
🐭 Just published online in JPS❣️
"Podoplanin hetero-insufficiency mice with inflammation in the jejunum demonstrates a good animal model of congenital protein-losing enteropathy"
-- Toshio Ohhashi et al.
🔗 [https://doi.org/10.1016/j.jphyss.2025.100031]
#MouseModel #JPhysiolSci
"Podoplanin hetero-insufficiency mice with inflammation in the jejunum demonstrates a good animal model of congenital protein-losing enteropathy"
-- Toshio Ohhashi et al.
🔗 [https://doi.org/10.1016/j.jphyss.2025.100031]
#MouseModel #JPhysiolSci