Read the full paper: Epitope-driven polyfunctional divergence of SARS-CoV-2 RBD antibodies

Rift Valley fever virus (RVFV) is a mosquito-borne virus that affects livestock and humans. The 4-segmented live-attenuated human vaccine candidate hRVFV-4s has shown a strong safety profile and excellent tolerability in healthy adults during a first-in-human clinical trial, while also eliciting both neutralizing antibody and T-cell responses. Recognizing the critical role of cellular immunity in vaccine-induced protection and immune durability, this study aimed to comprehensively characterize the cytokine secretion profile, the antigen-specific breadth of RVFV-specific T-cell responses and memory T-cell formation, elicited by a single dose of hRVFV-4s, up to 6 months post-vaccination.MethodsPeripheral blood mononuclear cells collected during the first-in-human clinical trial at 0, 7, 14, and 180 days post hRVFV-4s vaccination were analyzed for RVFV-specific T-cell responses using multiparametric flow cytometry and multiplex cytokine detection assays.ResultsA strong N-specific peripheral CD4+ and CD8+ T-cell response was detected among vaccinees, accompanied by Gn- and Gc-specific T cells, albeit the latter at comparatively lower frequencies. These responses were mediated by polyfunctional CD4+ and CD8+ T cells, which were detectable as early as at 2 weeks post-vaccination. The RVFV-specific T-cells were primarily of the effector memory phenotype and demonstrated cytokine secretion profiles characteristic of a T helper 1 (Th1)-type.ConclusionsThis study demonstrates that the cell-mediated immune response induced by a single dose of the hRVFV-4s vaccine is characterized by robust, virus-specific Th1-type CD4+ and CD8+ T-cell response. Together with previously reported virus-neutralizing antibody responses, these coordinated immune responses are expected to contribute to vaccine-mediated protection.

Scientific Reports - Cellular response evaluation in immunosuppressed individuals to help guide monoclonal antibody COVID-19 prophylactic treatment administration

Tuberculosis patients may benefit from host-directed therapy (HDT), but predictive biomarkers are needed. Mtb-specific polyfunctional and CD153+ CD4+ T cel

This article demonstrates the use of adenine base editor nickase activity for precise transgene cargo integration while simultaneously carrying out multiplex gene knockout. This single-step platform achieves efficient knock-in and >90% quadplex gene knockout without detectable translocations and low levels of off-target editing, producing highly functional, customizable CAR T cells.

Multivalent DNA plasmids, each encoding a diverse set of Mtb antigens, elicited robust polyfunctional T cell responses in naïve and BCG-primed mice. Boosting with lead construct, pESX, resulted in superior immunogenicity that correlated with prolonged pulmonary bacterial control in BCG-primed mice.

The polyfunctional pyrrole 3,5-dichloro-1H-pyrrole-2,4-dicarbaldehyde (2) was reacted with malononitrile and Meldrum’s acid in a regioselective manner to yield derivatives (4, 5, 6, 8, 9, and 10). Com...

Invasive Staphylococcus aureus infections have high mortality and reinfection rates, in part due to impaired development of enduring antibody-mediated immunity. The generation of pathogen-specific ant...

The parallel disruption of multiple genes coupled with targeted transgene insertion offers a powerful strategy for more effective and precise cell engineering. However, such orthogonal editing involves the induction of multiple DNA breaks, raising safety concerns related to the risks of chromosomal translocations. Here, we present a polyfunctional CRISPR-Cas9-based strategy that enables both transgene insertion and epigenetic silencing at distinct genomic loci in a single treatment without inducing reciprocal chromosomal translocations. This is accomplished through an optimized all-in-one epigenome editor equipped with a catalytically active Cas9, whose endonuclease activity is selectively disabled at epigenetically silenced loci using truncated gRNAs. As a proof of concept, we demonstrate that this platform enables efficient multi-locus editing, including functional replacement of the endogenous TCR with a tumor-selective one, targeted insertion of a prototypic CAR with either a selec

Nature Biomedical Engineering, Published online: 20 May 2026; doi:10.1038/s41551-026-01711-wAuthor Correction: Implanted microelectrode arrays in reinnervated muscles allow separation of neural drives from transferred polyfunctional nerves

Nur77 is associated with a TCF-1+ memory-like, polyfunctional profile in virus-specific human CD8+ T cells across acute-resolved, vaccinated, and chronic viral infections, and its pharmacologic modul....

Ma et al. identify a unique polyfunctional CD4+ T cell subset (poly6+ Th) in irAEs-CNS. These cells persistently react to neuroantigens (e.g., NMDAR1) and drive neuronal injury, and their abundance correlates with severe neuroinflammation and poor outcomes. IL-17 blockade alleviates pathology, suggesting a targeted therapeutic strategy.