Endothelial Cell MicroProteome
Computational pipeline MAPS
EC RiboTag-seq (Cdh5-CreERT2::Rpl22 HA/HA)🐭
👉>2k novel micropeptides (~50 aa) from cultured 👤🐭 ECs
👉361 micropeptides in 👤serum,
CRISPR library for 2890 smORFs➡️
248 impacting EC growth
#ResSq 2025
www.researchsquare.com/article/rs-4...
Computational pipeline MAPS
EC RiboTag-seq (Cdh5-CreERT2::Rpl22 HA/HA)🐭
👉>2k novel micropeptides (~50 aa) from cultured 👤🐭 ECs
👉361 micropeptides in 👤serum,
CRISPR library for 2890 smORFs➡️
248 impacting EC growth
#ResSq 2025
www.researchsquare.com/article/rs-4...
December 13, 2025 at 8:14 PM
Endothelial Cell MicroProteome
Computational pipeline MAPS
EC RiboTag-seq (Cdh5-CreERT2::Rpl22 HA/HA)🐭
👉>2k novel micropeptides (~50 aa) from cultured 👤🐭 ECs
👉361 micropeptides in 👤serum,
CRISPR library for 2890 smORFs➡️
248 impacting EC growth
#ResSq 2025
www.researchsquare.com/article/rs-4...
Computational pipeline MAPS
EC RiboTag-seq (Cdh5-CreERT2::Rpl22 HA/HA)🐭
👉>2k novel micropeptides (~50 aa) from cultured 👤🐭 ECs
👉361 micropeptides in 👤serum,
CRISPR library for 2890 smORFs➡️
248 impacting EC growth
#ResSq 2025
www.researchsquare.com/article/rs-4...
Unsure what to think about this. I wonder if an alternate explanation (ribosomes and translation are needed to release EJC's from mRNAs in the cytosol....
Ribosomal RNA transcription regulates splicing through ribosomal protein RPL22: Cell Chemical Biology www.cell.com/cell-chemica...
Ribosomal RNA transcription regulates splicing through ribosomal protein RPL22: Cell Chemical Biology www.cell.com/cell-chemica...
Ribosomal RNA transcription regulates splicing through ribosomal protein RPL22
Fan et al. uncovered a mechanism linking rRNA synthesis to spliceosomal regulation.
Using large-scale cancer cell line screens with specific RNA polymerase I inhibitors,
they identified a non-canonica...
www.cell.com
June 19, 2025 at 5:06 PM
Unsure what to think about this. I wonder if an alternate explanation (ribosomes and translation are needed to release EJC's from mRNAs in the cytosol....
Ribosomal RNA transcription regulates splicing through ribosomal protein RPL22: Cell Chemical Biology www.cell.com/cell-chemica...
Ribosomal RNA transcription regulates splicing through ribosomal protein RPL22: Cell Chemical Biology www.cell.com/cell-chemica...
RPL22は、リボソームタンパク質の一つで、28S rRNAと相互作用することが知られている。
本研究では、RPL22がリボソームの構成要素であるだけでなく、mRNAのスプライシング制御因子としてもはたらくことを示した。RPL22ハプロ不全のがん細胞では、RPL22L1やMDM4の選択スプライシングが変化して、がん化が促進される。そこへRNA pol1阻害剤を添加すると、rRNAの転写が抑制され、RPL22がリボソーム構成要素として取られない分、正常なスプライシング制御にはたらき、がん化が抑制されるというメカニズムが示唆された。
表紙絵は、mRNAの翻訳に携わるリボソームのイラスト。
本研究では、RPL22がリボソームの構成要素であるだけでなく、mRNAのスプライシング制御因子としてもはたらくことを示した。RPL22ハプロ不全のがん細胞では、RPL22L1やMDM4の選択スプライシングが変化して、がん化が促進される。そこへRNA pol1阻害剤を添加すると、rRNAの転写が抑制され、RPL22がリボソーム構成要素として取られない分、正常なスプライシング制御にはたらき、がん化が抑制されるというメカニズムが示唆された。
表紙絵は、mRNAの翻訳に携わるリボソームのイラスト。
August 27, 2025 at 2:14 AM
RPL22は、リボソームタンパク質の一つで、28S rRNAと相互作用することが知られている。
本研究では、RPL22がリボソームの構成要素であるだけでなく、mRNAのスプライシング制御因子としてもはたらくことを示した。RPL22ハプロ不全のがん細胞では、RPL22L1やMDM4の選択スプライシングが変化して、がん化が促進される。そこへRNA pol1阻害剤を添加すると、rRNAの転写が抑制され、RPL22がリボソーム構成要素として取られない分、正常なスプライシング制御にはたらき、がん化が抑制されるというメカニズムが示唆された。
表紙絵は、mRNAの翻訳に携わるリボソームのイラスト。
本研究では、RPL22がリボソームの構成要素であるだけでなく、mRNAのスプライシング制御因子としてもはたらくことを示した。RPL22ハプロ不全のがん細胞では、RPL22L1やMDM4の選択スプライシングが変化して、がん化が促進される。そこへRNA pol1阻害剤を添加すると、rRNAの転写が抑制され、RPL22がリボソーム構成要素として取られない分、正常なスプライシング制御にはたらき、がん化が抑制されるというメカニズムが示唆された。
表紙絵は、mRNAの翻訳に携わるリボソームのイラスト。
Ng, A. Y. E., Chan, S. N., Pek, J. W. (2024). Genetic compensation between ribosomal protein paralogs mediated by a cognate circular RNA. Cell Rep, 43(5):114228 www.cell.com/cell-reports...
Genetic compensation between ribosomal protein paralogs mediated by a cognate circular RNA
Ng et al. describe a mechanism of genetic crosstalk between two paralogs, RpL22 and
RpL22-like, mediated by a circRNA, circRpL22. This inter-regulation allows for the
modulation of each paralog’s expr...
www.cell.com
January 23, 2025 at 6:24 PM
Ng, A. Y. E., Chan, S. N., Pek, J. W. (2024). Genetic compensation between ribosomal protein paralogs mediated by a cognate circular RNA. Cell Rep, 43(5):114228 www.cell.com/cell-reports...
3/4 We identify novel substrates like #RPL22, #EIF3D and #PCM1, opening new doors to more functional insights into these #posttranslationalmodifications so far implicated primarily in #microtubule regulation. Their role in #cellbiology seems more than what is thought to be.
June 12, 2026 at 1:50 AM
3/4 We identify novel substrates like #RPL22, #EIF3D and #PCM1, opening new doors to more functional insights into these #posttranslationalmodifications so far implicated primarily in #microtubule regulation. Their role in #cellbiology seems more than what is thought to be.
Online now! #chembiol
Ribosomal RNA transcription regulates splicing through ribosomal protein RPL22
Fan et al. uncovered a mechanism linking rRNA synthesis to spliceosomal regulation. Using large-scale cancer cell line screens with specific RNA polymerase I inhibitors, they identified a non-canonical ribotoxic stress pathway mediated by RPL22, RPL22L1, and MDM4, with RPL22 acting as a master splicing regulator coordinated with rRNA synthesis.
dlvr.it
June 18, 2025 at 3:31 PM
Online now! #chembiol
Intronic RNA of yeast RPL22 paralogs acts as an allosteric switch https://www.biorxiv.org/content/10.64898/2026.02.10.704854v1
February 12, 2026 at 5:20 AM
Intronic RNA of yeast RPL22 paralogs acts as an allosteric switch https://www.biorxiv.org/content/10.64898/2026.02.10.704854v1
Ribosomal RNA transcription regulates splicing through ribosomal protein RPL22
www.cell.com/cell-chemica...
www.cell.com/cell-chemica...
Ribosomal RNA transcription regulates splicing through ribosomal protein RPL22
Fan et al. uncovered a mechanism linking rRNA synthesis to spliceosomal regulation.
Using large-scale cancer cell line screens with specific RNA polymerase I inhibitors,
they identified a non-canonica...
www.cell.com
July 1, 2025 at 1:59 AM
Ribosomal RNA transcription regulates splicing through ribosomal protein RPL22
www.cell.com/cell-chemica...
www.cell.com/cell-chemica...
Ribosomal protein control of hematopoietic stem cell transformation through regulation of metabolism
Ribosomal protein control of hematopoietic stem cell transformation through regulation of metabolism
Harris et al. demonstrate that insufficiency of the ribosomal protein Rpl22 controls the transformation potential of hematopoietic stem cells (HSCs) not by altering protein synthesis but instead through regulating lipid metabolism, including both fatty acid oxidation and triglyceride synthesis.
dlvr.it
December 27, 2025 at 12:23 AM
Ribosomal protein control of hematopoietic stem cell transformation through regulation of metabolism
(continuação 2)
A descoberta revelou que a proteína ribossômica RPL22 atua como reguladora crítica no crescimento do tumor e na eficácia de tratamentos. Os testes pré-clínicos indicam que novos medicamentos podem atingir cânceres de difícil resposta e alta letalidade de forma personalizada.
A descoberta revelou que a proteína ribossômica RPL22 atua como reguladora crítica no crescimento do tumor e na eficácia de tratamentos. Os testes pré-clínicos indicam que novos medicamentos podem atingir cânceres de difícil resposta e alta letalidade de forma personalizada.
May 4, 2026 at 1:25 PM
(continuação 2)
A descoberta revelou que a proteína ribossômica RPL22 atua como reguladora crítica no crescimento do tumor e na eficácia de tratamentos. Os testes pré-clínicos indicam que novos medicamentos podem atingir cânceres de difícil resposta e alta letalidade de forma personalizada.
A descoberta revelou que a proteína ribossômica RPL22 atua como reguladora crítica no crescimento do tumor e na eficácia de tratamentos. Os testes pré-clínicos indicam que novos medicamentos podem atingir cânceres de difícil resposta e alta letalidade de forma personalizada.
Research reveals that reduced expression of ribosomal protein RPL22 is associated with worse outcomes in myelodysplastic syndrome and acute myelogenous leukemia.
🔗 Source: https://doi.org/10.1101/2023.05.31.543132
🔗 Source: https://doi.org/10.1101/2023.05.31.543132
August 1, 2026 at 3:55 AM
Research reveals that reduced expression of ribosomal protein RPL22 is associated with worse outcomes in myelodysplastic syndrome and acute myelogenous leukemia.
🔗 Source: https://doi.org/10.1101/2023.05.31.543132
🔗 Source: https://doi.org/10.1101/2023.05.31.543132
A preprint using: B6J.129(Cg)-Rpl22#InternationalMouseSt was published.
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #RRID #methodsmatter
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #RRID #methodsmatter
Mesolimbic and mesocortical pathways differentially support fentanyl-context associations
www.biorxiv.org
July 20, 2026 at 12:00 PM
A preprint using: B6J.129(Cg)-Rpl22#InternationalMouseSt was published.
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #RRID #methodsmatter
SciScore made a table with this resource, see “Automated Services” module (download as csv, xml or #jats) #RRID #methodsmatter
➡️ Die Forschungspublikation der "Johns Hopkins University School of Medicine" (JHUSOM) 🇺🇸 im Fachmagazin "Cell Chemical Biology"
www.cell.com/cell-chemica... 2/2
www.cell.com/cell-chemica... 2/2
Ribosomal RNA transcription regulates splicing through ribosomal protein RPL22
Fan et al. uncovered a mechanism linking rRNA synthesis to spliceosomal regulation.
Using large-scale cancer cell line screens with specific RNA polymerase I inhibitors,
they identified a non-canonica...
www.cell.com
July 6, 2025 at 12:40 AM
➡️ Die Forschungspublikation der "Johns Hopkins University School of Medicine" (JHUSOM) 🇺🇸 im Fachmagazin "Cell Chemical Biology"
www.cell.com/cell-chemica... 2/2
www.cell.com/cell-chemica... 2/2
The transition from monocyte to tissue-resident macrophage requires DHPS
->Nature | More from Lil Dr Glen EcoChat at BigEarthData.ai
->Nature | More from Lil Dr Glen EcoChat at BigEarthData.ai
The transition from monocyte to tissue-resident macrophage requires DHPS
WT C57BL/6 and CD45.1 SJL mice, mice expressing Cre recombinase (LysM–Cre) under the control of the lysozyme (LysM) promoter, CX3CR1–ERT2 inducible Cre, Rosa26-eYFP (R26YFP) mice and RiboTag mice61 harbouring a modified allele of Rpl22 (Rpl22-HA) induced by the action of...
www.nature.com
January 22, 2026 at 2:57 AM
Intronic RNA of yeast RPL22 paralogs acts as an allosteric switch https://www.biorxiv.org/content/10.64898/2026.02.10.704854v1
February 12, 2026 at 5:20 AM
Intronic RNA of yeast RPL22 paralogs acts as an allosteric switch https://www.biorxiv.org/content/10.64898/2026.02.10.704854v1
Ribosomal RNA transcription governs splicing through ribosomal protein RPL22 https://www.biorxiv.org/content/10.1101/2024.08.15.608201v1
Ribosomal RNA transcription governs splicing through ribosomal protein RPL22 https://www.biorxiv.org/content/10.1101/2024.08.15.608201v1
Ribosome biosynthesis is a cancer vulnerability executed by targeting RNA polymerase I (Pol I) trans
www.biorxiv.org
August 17, 2024 at 5:17 AM
Ribosomal RNA transcription governs splicing through ribosomal protein RPL22 https://www.biorxiv.org/content/10.1101/2024.08.15.608201v1
RPL22 is a tumor suppressor in MSI-high cancers and a key splicing regulator of MDM4 https://www.biorxiv.org/content/10.1101/2023.12.10.570873v1
RPL22 is a tumor suppressor in MSI-high cancers and a key splicing regulator of MDM4 https://www.biorxiv.org/content/10.1101/2023.12.10.570873v1
Microsatellite instability high (MSI-H) tumors are malignant tumors that, despite harboring a high m
www.biorxiv.org
December 11, 2023 at 8:07 PM
RPL22 is a tumor suppressor in MSI-high cancers and a key splicing regulator of MDM4 https://www.biorxiv.org/content/10.1101/2023.12.10.570873v1
Ribosomal RNA transcription governs splicing through ribosomal protein RPL22 https://www.biorxiv.org/content/10.1101/2024.08.15.608201v1
Ribosomal RNA transcription governs splicing through ribosomal protein RPL22 https://www.biorxiv.org/content/10.1101/2024.08.15.608201v1
Ribosome biosynthesis is a cancer vulnerability executed by targeting RNA polymerase I (Pol I) trans
www.biorxiv.org
August 17, 2024 at 5:17 AM
Ribosomal RNA transcription governs splicing through ribosomal protein RPL22 https://www.biorxiv.org/content/10.1101/2024.08.15.608201v1
RPL22 is a tumor suppressor in MSI-high cancers and a key splicing regulator of MDM4 https://www.biorxiv.org/content/10.1101/2023.12.10.570873v1
RPL22 is a tumor suppressor in MSI-high cancers and a key splicing regulator of MDM4 https://www.biorxiv.org/content/10.1101/2023.12.10.570873v1
Microsatellite instability high (MSI-H) tumors are malignant tumors that, despite harboring a high m
www.biorxiv.org
December 11, 2023 at 8:07 PM
RPL22 is a tumor suppressor in MSI-high cancers and a key splicing regulator of MDM4 https://www.biorxiv.org/content/10.1101/2023.12.10.570873v1