I am trying and failing to find a RTqPCR triplex assay for research use for SARS-CoV-2, flu and RSV - everything is a branded, automated assay. Can anyone recommend a research set of primers and probes?
October 27, 2026 at 11:30 AM
I am trying and failing to find a RTqPCR triplex assay for research use for SARS-CoV-2, flu and RSV - everything is a branded, automated assay. Can anyone recommend a research set of primers and probes?
Bound guide #RNA can stop a conventional RT before it reaches your target region, inflating apparent #CRISPR knockdown in #RTqPCR.
UltraMarathonRT's strand displacement activity means it can read straight through it — accurate KD/KO quantitation validated across Csm, PspCas13b & CasRx.
🔗⬇
UltraMarathonRT's strand displacement activity means it can read straight through it — accurate KD/KO quantitation validated across Csm, PspCas13b & CasRx.
🔗⬇
September 10, 2026 at 5:01 PM
Congratulations to Leslie Watkins, Alan Zhu & senior author Bin Wu at Johns Hopkins on identifying a pervasive #RTqPCR artifact that inflates apparent knockdown across RNA-targeting #CRISPR systems (Csm, PspCas13b, CasRx).
Rigorous work the CRISPR field needed. 🎉
Rigorous work the CRISPR field needed. 🎉
A pervasive RT–qPCR artifact inflates RNA knockdown by RNA-targeting CRISPR - @binwu1.bsky.social @hopkinsmedicine.bsky.social www.nature.com/articles/s41...
A pervasive RT–qPCR artifact inflates RNA knockdown by RNA-targeting CRISPR - Nature Biotechnology
A quantification artifact confounds CRISPR-mediated RNA knockdown.
www.nature.com
September 1, 2026 at 5:20 PM
Any folks in the #Drosophila feed have experience with gene expression via RTqPCR? I've had a good deal of trouble with amplification and curious if folks have run into similar issues
(will share details and protocols)
(will share details and protocols)
November 26, 2024 at 2:30 AM
Any folks in the #Drosophila feed have experience with gene expression via RTqPCR? I've had a good deal of trouble with amplification and curious if folks have run into similar issues
(will share details and protocols)
(will share details and protocols)
First detection of Jingmen tick virus in Corsica with a new generic RTqPCR system
www.nature.com/articles/s44...
www.nature.com/articles/s44...
First detection of Jingmen tick virus in Corsica with a new generic RTqPCR system - npj Viruses
npj Viruses - First detection of Jingmen tick virus in Corsica with a new generic RTqPCR system
www.nature.com
September 30, 2024 at 2:28 PM
First detection of Jingmen tick virus in Corsica with a new generic RTqPCR system
www.nature.com/articles/s44...
www.nature.com/articles/s44...
Day 7 in the Lab:
Excitement is still there!
I am almost finished with my Western Blots. Thank god, they all worked out really well, and I got overall really nice bands.
Next week's gonna be about more focused on RNA and RTqPCR. I'm very excited for that as well 😁
Excitement is still there!
I am almost finished with my Western Blots. Thank god, they all worked out really well, and I got overall really nice bands.
Next week's gonna be about more focused on RNA and RTqPCR. I'm very excited for that as well 😁
July 9, 2025 at 9:05 PM
Day 7 in the Lab:
Excitement is still there!
I am almost finished with my Western Blots. Thank god, they all worked out really well, and I got overall really nice bands.
Next week's gonna be about more focused on RNA and RTqPCR. I'm very excited for that as well 😁
Excitement is still there!
I am almost finished with my Western Blots. Thank god, they all worked out really well, and I got overall really nice bands.
Next week's gonna be about more focused on RNA and RTqPCR. I'm very excited for that as well 😁
October 21, 2025 at 9:23 PM
RTqPCR ama Antonelli’ye nazar değiyor
May 19, 2025 at 12:14 PM
RTqPCR ama Antonelli’ye nazar değiyor
#MPB
Study identifies optimal reference genes for qRT-PCR in Dendrobium nobile under different stresses & tissues, ensuring accurate gene expression analysis.
Details: https://maxapress.com/article/doi/10.48130/mpb-0024-0031
#Dendrobium #RTqPCR
Study identifies optimal reference genes for qRT-PCR in Dendrobium nobile under different stresses & tissues, ensuring accurate gene expression analysis.
Details: https://maxapress.com/article/doi/10.48130/mpb-0024-0031
#Dendrobium #RTqPCR
May 29, 2026 at 3:45 AM
#MPB
Study identifies optimal reference genes for qRT-PCR in Dendrobium nobile under different stresses & tissues, ensuring accurate gene expression analysis.
Details: https://maxapress.com/article/doi/10.48130/mpb-0024-0031
#Dendrobium #RTqPCR
Study identifies optimal reference genes for qRT-PCR in Dendrobium nobile under different stresses & tissues, ensuring accurate gene expression analysis.
Details: https://maxapress.com/article/doi/10.48130/mpb-0024-0031
#Dendrobium #RTqPCR
#VegetableRes
Study identifies CaActin1 & CaGAPDH2 as optimal RT-qPCR reference genes for pepper across tissues & stress, enhancing gene expression accuracy.
Details: https://maxapress.com/article/doi/10.48130/vegres-0025-0045
#Pepper #RTqPCR
Study identifies CaActin1 & CaGAPDH2 as optimal RT-qPCR reference genes for pepper across tissues & stress, enhancing gene expression accuracy.
Details: https://maxapress.com/article/doi/10.48130/vegres-0025-0045
#Pepper #RTqPCR
March 17, 2026 at 10:29 AM
#VegetableRes
Study identifies CaActin1 & CaGAPDH2 as optimal RT-qPCR reference genes for pepper across tissues & stress, enhancing gene expression accuracy.
Details: https://maxapress.com/article/doi/10.48130/vegres-0025-0045
#Pepper #RTqPCR
Study identifies CaActin1 & CaGAPDH2 as optimal RT-qPCR reference genes for pepper across tissues & stress, enhancing gene expression accuracy.
Details: https://maxapress.com/article/doi/10.48130/vegres-0025-0045
#Pepper #RTqPCR
You may be getting biased or inaccurate results from your RT-qPCR kit and not even know it.
Our new white paper shows how UltraMarathonRT® delivers robust, reliable results regardless of primer choice and outperforms leading retroviral RTs.
🔗Link in comments.
#RTqPCR #qPCR #RNASky
Our new white paper shows how UltraMarathonRT® delivers robust, reliable results regardless of primer choice and outperforms leading retroviral RTs.
🔗Link in comments.
#RTqPCR #qPCR #RNASky
September 23, 2025 at 3:48 PM
Watch this 10-minute session from #ABRF2025 and learn from our Sr. Scientist, Dr. George Maio, as he reveals commonly hidden RNAs with UltraMarathonRT, a new, ultra-processive reverse transcriptase.
Watch today! bit.ly/44l4ZDk
#ABRF #RNAseq #RTqPCR #RNAresearch #RNAsky
Watch today! bit.ly/44l4ZDk
#ABRF #RNAseq #RTqPCR #RNAresearch #RNAsky
May 7, 2025 at 3:40 PM
Watch this 10-minute session from #ABRF2025 and learn from our Sr. Scientist, Dr. George Maio, as he reveals commonly hidden RNAs with UltraMarathonRT, a new, ultra-processive reverse transcriptase.
Watch today! bit.ly/44l4ZDk
#ABRF #RNAseq #RTqPCR #RNAresearch #RNAsky
Watch today! bit.ly/44l4ZDk
#ABRF #RNAseq #RTqPCR #RNAresearch #RNAsky
Say goodbye to 2024 AND variability in qPCR data with the uMRT RT-qPCR Kit! With consistent Ct values across RT primer types, RNA complexities, and amplicon locations, uMRT empowers reliable RNA quantification - even for challenging samples!
🔗https://bit.ly/4h5XanX
#GeneExpression #RTqPCR #RNA
🔗https://bit.ly/4h5XanX
#GeneExpression #RTqPCR #RNA
January 9, 2025 at 6:23 PM
Say goodbye to 2024 AND variability in qPCR data with the uMRT RT-qPCR Kit! With consistent Ct values across RT primer types, RNA complexities, and amplicon locations, uMRT empowers reliable RNA quantification - even for challenging samples!
🔗https://bit.ly/4h5XanX
#GeneExpression #RTqPCR #RNA
🔗https://bit.ly/4h5XanX
#GeneExpression #RTqPCR #RNA
Dynamics of Histoplasma fungal load in people living with HIV with disseminated histoplasmosis under treatment with liposomal amphotericin B
#IDSky #MedMycoSky #HIVSky
www.clinicalmicrobiologyandinfection.com/article/S119...
#IDSky #MedMycoSky #HIVSky
www.clinicalmicrobiologyandinfection.com/article/S119...
December 9, 2024 at 12:20 PM
Dynamics of Histoplasma fungal load in people living with HIV with disseminated histoplasmosis under treatment with liposomal amphotericin B
#IDSky #MedMycoSky #HIVSky
www.clinicalmicrobiologyandinfection.com/article/S119...
#IDSky #MedMycoSky #HIVSky
www.clinicalmicrobiologyandinfection.com/article/S119...
Never done a southern! I do mostly RTqPCR.
November 22, 2023 at 1:29 PM
Never done a southern! I do mostly RTqPCR.
Collect #NeuralCrest Cells for #SingleCell #RNAseq from any vertebrate.
Our new paper, present an antibody-guided #FACS method to collect NCCs. No trans-genesis or in ovo manipulation required.
Demonstrated in wall #lizard #embryos using #microscopy, #RTqPCR and #scRNAseq.
doi.org/10.1111/ede....
Our new paper, present an antibody-guided #FACS method to collect NCCs. No trans-genesis or in ovo manipulation required.
Demonstrated in wall #lizard #embryos using #microscopy, #RTqPCR and #scRNAseq.
doi.org/10.1111/ede....
February 19, 2026 at 7:23 AM
Collect #NeuralCrest Cells for #SingleCell #RNAseq from any vertebrate.
Our new paper, present an antibody-guided #FACS method to collect NCCs. No trans-genesis or in ovo manipulation required.
Demonstrated in wall #lizard #embryos using #microscopy, #RTqPCR and #scRNAseq.
doi.org/10.1111/ede....
Our new paper, present an antibody-guided #FACS method to collect NCCs. No trans-genesis or in ovo manipulation required.
Demonstrated in wall #lizard #embryos using #microscopy, #RTqPCR and #scRNAseq.
doi.org/10.1111/ede....
Development of a #SYBR Green-Based #RTqPCR Assay for the Detection and Quantification of #LoneStarVirus.
Read More: tinyurl.com/3bac3tdd
#arbovirus #tickborne #Zoonoses #Zoonosis #Zoonotics #Zoonoticdiseases @scienceopen.bsky.social
Read More: tinyurl.com/3bac3tdd
#arbovirus #tickborne #Zoonoses #Zoonosis #Zoonotics #Zoonoticdiseases @scienceopen.bsky.social
Development of a SYBR Green-Based RT-qPCR Assay for the Detection and Quantification of Lone Star Virus
Two SYBR green-based RT-qPCRs were developed and validated for the detection and quantification of Lone Star Virus. Two primer sets were evaluated for reproducibility, specificity, and sensitivity.
tinyurl.com
January 23, 2025 at 1:29 PM
Development of a #SYBR Green-Based #RTqPCR Assay for the Detection and Quantification of #LoneStarVirus.
Read More: tinyurl.com/3bac3tdd
#arbovirus #tickborne #Zoonoses #Zoonosis #Zoonotics #Zoonoticdiseases @scienceopen.bsky.social
Read More: tinyurl.com/3bac3tdd
#arbovirus #tickborne #Zoonoses #Zoonosis #Zoonotics #Zoonoticdiseases @scienceopen.bsky.social
And in a phase 2 trial of LAmB, 63/105 (64%) had +RTqPCR at diagnosis. ↗️➡️whole nucleic acid load in blood (measured by RTqPCR) during antifungal treatment associated with death at various timepoints
www.clinicalmicrobiologyandinfection.com/article/S119...
www.clinicalmicrobiologyandinfection.com/article/S119...
December 27, 2024 at 10:41 AM
And in a phase 2 trial of LAmB, 63/105 (64%) had +RTqPCR at diagnosis. ↗️➡️whole nucleic acid load in blood (measured by RTqPCR) during antifungal treatment associated with death at various timepoints
www.clinicalmicrobiologyandinfection.com/article/S119...
www.clinicalmicrobiologyandinfection.com/article/S119...