#T3SS
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plosbiology.org
How do intracellular #bacteria breach vacuoles to enter the host cytosol? @leaswistak.bsky.social @matthijnvos.bsky.social @enningalab.bsky.social &co show that #Shigella uses its T3SS system to damage endomembranes via mechanoporation to initiate cytosolic access @plosbiology.org 🧪 plos.io/4jXrr9Z
Left: Group of three T3SS localizing in the same zone of the bacteria surface. T3SS n°1 and 2 contact, deform, and perforate the vacuole membrane at the same spot. T3SS n°1 needle (83 nm) is long and seems to deviate from basal body axis showing that strong constraints between T3SS and the vacuole at are play. The locally relaxed vacuole to bacteria space (~50–55 nm) potentially limits additional damage by the T3SS n°3 needle (56 nm) that only slightly deforms the membrane. Right: Model of initial vacuolar membrane breaching by T3SS-induced mechanoporation. Shigella actively enters host cells in a specialized endomembrane compartment, the vacuole, that is rapidly injured and ruptured for cytosolic access. After host cell entry, the vacuole membrane is intact and tightly juxtaposed to the surface of Shigella, permitting contact between T3SSs and the endomembrane. In the authors’ proposed model, vacuole membrane injury is supported both by the vacuole tightness and the length of individual T3SSs.
May 6, 2025 at 12:46 PM
plosbiology.org
How do intracellular #bacteria breach vacuoles to enter the host cytosol? @leaswistak.bsky.social @matthijnvos.bsky.social @enningalab.bsky.social &co show that #Shigella uses its T3SS system to damage endomembranes via mechanoporation to initiate cytosolic access @plosbiology.org 🧪 plos.io/4jXrr9Z
Left: Group of three T3SS localizing in the same zone of the bacteria surface. T3SS n°1 and 2 contact, deform, and perforate the vacuole membrane at the same spot. T3SS n°1 needle (83 nm) is long and seems to deviate from basal body axis showing that strong constraints between T3SS and the vacuole at are play. The locally relaxed vacuole to bacteria space (~50–55 nm) potentially limits additional damage by the T3SS n°3 needle (56 nm) that only slightly deforms the membrane. Right: Model of initial vacuolar membrane breaching by T3SS-induced mechanoporation. Shigella actively enters host cells in a specialized endomembrane compartment, the vacuole, that is rapidly injured and ruptured for cytosolic access. After host cell entry, the vacuole membrane is intact and tightly juxtaposed to the surface of Shigella, permitting contact between T3SSs and the endomembrane. In the authors’ proposed model, vacuole membrane injury is supported both by the vacuole tightness and the length of individual T3SSs.
May 2, 2025 at 5:00 PM
plosbiology.org
How do intracellular #bacteria breach vacuoles to enter the host cytosol? @leaswistak.bsky.social @matthijnvos.bsky.social @enningalab.bsky.social &co show that #Shigella uses its T3SS system to damage endomembranes via mechanoporation to initiate cytosolic access @plosbiology.org 🧪 plos.io/4jXrr9Z
Left: Group of three T3SS localizing in the same zone of the bacteria surface. T3SS n°1 and 2 contact, deform, and perforate the vacuole membrane at the same spot. T3SS n°1 needle (83 nm) is long and seems to deviate from basal body axis showing that strong constraints between T3SS and the vacuole at are play. The locally relaxed vacuole to bacteria space (~50–55 nm) potentially limits additional damage by the T3SS n°3 needle (56 nm) that only slightly deforms the membrane. Right: Model of initial vacuolar membrane breaching by T3SS-induced mechanoporation. Shigella actively enters host cells in a specialized endomembrane compartment, the vacuole, that is rapidly injured and ruptured for cytosolic access. After host cell entry, the vacuole membrane is intact and tightly juxtaposed to the surface of Shigella, permitting contact between T3SSs and the endomembrane. In the authors’ proposed model, vacuole membrane injury is supported both by the vacuole tightness and the length of individual T3SSs.
May 2, 2025 at 12:35 PM
anagramm11.bsky.social
Da ist noch einer:

bsky.app/profile/t3ss...
bsky.app
February 19, 2026 at 10:19 PM
nizet.bsky.social
in lively festivals each spring throughout Catalonia (Spain), microbiology professors and their postdocs thrill crowds by reenacting the assembly of a type 3 secretion system (T3SS)
November 26, 2024 at 1:51 PM
abaumler.bsky.social
A Salmonella T3SS-2 mutant grows fine in spleen macrophages, contradicting tissue culture dogma (PMID: 23236281). This observation was largely ignored, but adding certain carbon sources rescues growth in cultured macrophages, hinting that T3SS-2 may be doing something entirely different in vivo.
pescoll.bsky.social Pedro Escoll @pescoll.bsky.social · May 16
New pre-print in the lab! Beautiful work by Francisco Garcia-Rodriguez in collab with @kamovalenz.bsky.social
and @joaquinbernal.bsky.social : when macrophages are provided with specific nutrients, Salmonella can bypass its requirement of the T3SS to replicate, a process that occurs within the SCV
biorxiv-microbiol.bsky.social bioRxiv Microbiology @biorxiv-microbiol.bsky.social · May 16
Provision of Preferred Nutrients to Macrophages Enables Salmonella to Replicate Intracellularly Without Relying on Type III Secretion Systems https://www.biorxiv.org/content/10.1101/2025.05.15.653970v1
August 9, 2025 at 12:18 AM
plosbiology.org
How do intracellular #bacteria breach vacuoles to enter the host cytosol? @leaswistak.bsky.social @matthijnvos.bsky.social @enningalab.bsky.social &co show that #Shigella uses its T3SS system to damage endomembranes via mechanoporation to initiate cytosolic access @plosbiology.org 🧪 plos.io/4jXrr9Z
Left: Group of three T3SS localizing in the same zone of the bacteria surface. T3SS n°1 and 2 contact, deform, and perforate the vacuole membrane at the same spot. T3SS n°1 needle (83 nm) is long and seems to deviate from basal body axis showing that strong constraints between T3SS and the vacuole at are play. The locally relaxed vacuole to bacteria space (~50–55 nm) potentially limits additional damage by the T3SS n°3 needle (56 nm) that only slightly deforms the membrane. Right: Model of initial vacuolar membrane breaching by T3SS-induced mechanoporation. Shigella actively enters host cells in a specialized endomembrane compartment, the vacuole, that is rapidly injured and ruptured for cytosolic access. After host cell entry, the vacuole membrane is intact and tightly juxtaposed to the surface of Shigella, permitting contact between T3SSs and the endomembrane. In the authors’ proposed model, vacuole membrane injury is supported both by the vacuole tightness and the length of individual T3SSs.
May 6, 2025 at 7:47 AM
nizet.bsky.social
Antivirulence strategies can spare the microbiome and minimize AMR selective pressure

New discovery of small molecule C26 that blocks 𝘚𝘢𝘭𝘮𝘰𝘯𝘦𝘭𝘭𝘢 host cell invasion by targeting HilD—a master regulator its secretion systems (T1SS, T3SS-1, T3SS-2)

Don’t destroy — DISARM

Wagner Lab Tübingen Sci Adv
April 15, 2025 at 1:09 PM
t3sss.bsky.social
Flagellar system translocates T3SS effectors critical for Salmonella infection

www.biorxiv.org/content/10.6...
Flagellar system translocates T3SS effectors critical for Salmonella infection
Type 3 secretion system (T3SS) injectisomes, the essential virulence factors of many bacterial pathogens, are thought to have evolved from the flagellar T3SS. Despite their high degree of structural s...
www.biorxiv.org
June 8, 2026 at 10:20 PM
anotherjewess.bsky.social
bsky.app/profile/t3ss...
bsky.app
February 19, 2026 at 9:17 PM
anotherjewess.bsky.social
Die Person macht Weiter :

bsky.app/profile/t3ss...
bsky.app
February 19, 2026 at 9:10 PM
diepold-lab.bsky.social
What if even the core of bacterial nanomachines wasn’t static?

We found that the T3SS core protein SctD in Yersinia dynamically exchanges subunits — and this flexibility is essential for proper assembly & function.

🔗 www.nature.com/articles/s41...

@kit.edu @t3sss.bsky.social
November 12, 2025 at 4:21 PM
joshnewson.bsky.social
Excited to share this story describing new tools for studying Salmonella virulence. We engineered six multimutant strains to be deficient for different sets of SPI-2 T3SS effectors. We think there's lots of potential for new discoveries using these strains www.biorxiv.org/content/10.1...
Salmonella multimutants enable efficient identification of SPI-2 effector protein function in gut inflammation and systemic colonization
Salmonella enterica spp. rely on translocation of effector proteins through the SPI-2 encoded type III secretion system (T3SS) to achieve pathogenesis. More than 30 effectors contribute to manipulatio...
www.biorxiv.org
December 15, 2024 at 3:48 PM
drdorsalomon.bsky.social
Preprint 🚨🦠
Check out our new collaboration with the van Kessl and Gerlic labs
On the #T3SS of #Vibrio campbellii. New effectors unique to this species!

www.biorxiv.org/content/10.6...
June 4, 2026 at 1:09 PM
euroxanth.eu
Starting my BlueSky experience with last week's poster about our community-curated #DokuWiki resource on #diagnostics, #diversity, #pathogenicity and genetic control of #xanthomonads: www.researchgate.net/publication/...
#T3SS | #effector | #resistance | #xanthomonas
January 19, 2025 at 4:20 PM
pescoll.bsky.social
New pre-print in the lab! Beautiful work by Francisco Garcia-Rodriguez in collab with @kamovalenz.bsky.social
and @joaquinbernal.bsky.social : when macrophages are provided with specific nutrients, Salmonella can bypass its requirement of the T3SS to replicate, a process that occurs within the SCV
biorxiv-microbiol.bsky.social bioRxiv Microbiology @biorxiv-microbiol.bsky.social · May 16
Provision of Preferred Nutrients to Macrophages Enables Salmonella to Replicate Intracellularly Without Relying on Type III Secretion Systems https://www.biorxiv.org/content/10.1101/2025.05.15.653970v1
May 16, 2025 at 8:04 AM
diepold-lab.bsky.social
T3SS activity comes at a cost—bacteria that use the system pay with reduced growth. We found how Yersinia balance costs and benefits: at higher densities, they actively suppress T3SS activity and adhesion, switching from colonization to replication and dissemination.
doi.org/10.1371/jour...
@kit.edu
Yersinia actively downregulates type III secretion and adhesion at higher cell densities
Author summary Bacteria can use the type III secretion system (T3SS), a molecular syringe-like device, to manipulate host cells by injecting effector proteins. Yersinia enterocolitica, a pathogenic ba...
doi.org
September 2, 2025 at 8:34 PM
diepold-lab.bsky.social
Secreting bacteria undergo drastic changes.
We found that in Yersinia, T3SS activation triggers rapid, large-scale reorganisation of chromosomal and plasmid DNA. This links secretion to growth inhibition-revealing a new connection between virulence and bacterial cell biology.
doi.org/10.1016/j.ce...
November 24, 2025 at 10:56 PM
juliensluneau.bsky.social
Great to see this out! 🎉 Big congratulations to @type3lab.bsky.social for leading the project and to all involved!

"Pseudomonas syringae subpopulations cooperate by coordinating flagellar and T3SS spatiotemporal dynamics to facilitate plant infection" 🌱🦠 rdcu.be/egczU

🧵⬇️ #MicroSky #PlantScience
April 4, 2025 at 9:55 AM
pescoll.bsky.social
New paper from the team in Plos Pathogens @plos.org
: providing macrophages with preferred nutrients lets Salmonella replicate even without its Type III Secretion Systems (T3SS). Work by Francisco Garcia-Rodriguez & Paula Martinez, with @kamovalenz.bsky.social and @joaquinbernal.bsky.social 🧵1/9
July 10, 2026 at 9:49 AM
diepold-lab.bsky.social
Using live-cell microscopy, we uncovered distinct bacterial subpopulations that primarily use certain combinations of these nanomachines:
• some prioritize motility & T3SS
• others shift to T6SS for antagonism
All tuned by the usual suspect: cyclic di-GMP.
That's how we think Pseudomonas aeruginosa uses the T3SS, H1-T6SS, and flagellum during infection.
August 20, 2025 at 2:46 PM
abaumler.bsky.social
A Salmonella T3SS-2 mutant grows fine in spleen macrophages, contradicting tissue culture dogma (PMID:23236281). This was largely ignored, but adding certain carbon sources rescues growth in cultured macrophages, hinting T3SS-2 may be doing something different in vivo www.biorxiv.org/content/10.1...
Provision of Preferred Nutrients to Macrophages Enables Salmonella to Replicate Intracellularly Without Relying on Type III Secretion Systems
Intracellular survival and replication within macrophages are key virulence determinants of Salmonella enterica serovar Typhimurium. This phenomenon is traditionally attributed to the activity of its ...
www.biorxiv.org
August 9, 2025 at 12:05 AM
enningalab.bsky.social
Our latest paper is out! Congratulations to @leaswistak.bsky.social et al for this amazing #cryoET work that elucidates the initial stages of #T3SS dependent vacuolar damage during #Shigella infection. We will do a deep-dive later! journals.plos.org/plosbiology/...
May 6, 2025 at 1:06 PM
gittacoaker.bsky.social
Tomato phytoalexin disrupts bacterial type III secretion system.

A widespread plant defense compound disarms bacterial type III injectisome assembly | Science www.science.org/doi/10.1126/...
A widespread plant defense compound disarms bacterial type III injectisome assembly
Numerous gram-negative bacterial pathogens employ the type III secretion system (T3SS), a multiprotein injectisome, to deliver virulence proteins into host cells and cause diseases. We uncover erucami...
www.science.org
February 28, 2025 at 5:50 PM