A staff report asked the Southern California Association of Governments Transportation Committee to recommend Regional Council approval of 18 project nominations seeking $746,371,755 in Trade Corridor Enhancement Program funding; total project costs listed exceed $3.04 billion. The nominations include 10 projects led or partnered by Caltrans.

Sf21 cells in Sf-900 II serum-free medium (Gibco, 11497-013) were co-infected at a multiplicity of infection of 1 for both His-tagged METTL1 and Flag-tagged WDR4 baculovirus preparations. The infected cultures were incubated for 72 h at 110 rpm and 27 °C. Cells were collected by centrifugation at 3,400g for 15 min at 4 °C and the pellets were stored at −80 °C. The pellets were lysed in 25 mM Tris pH 8.0 (Invitrogen, 15568025), 300 mM NaCl (Merck, S6546), 1 mM tris(2-carboxyethyl)phosphine (TCEP) (Thermo Fisher Scientific, 20490), 5% glycerol (Thermo Fisher Scientific, 327255000), 0.25% CHAPS (Thermo Fisher Scientific, 28300), 1 mM phenylmethylsulfonyl fluoride (PMSF) (Thermo Fisher Scientific, 36978) supplemented with protease inhibitor tablets (Roche, 4693124001) and 10 U ml−1 benzonase (Merck, E8263). Homogenization was achieved by two cycles at 20 kilopounds per square inch using an IKA high pressure homogenizer. Lysates were briefly centrifuged in between cycles (2,000 rpm for 15 min) to remove foam and finally cleared by ultracentrifugation (45 min at 150,000g, 4 °C). The protein complex was purified sequentially by immobilized metal affinity chromatography (IMAC), desalting, ion-exchange chromatography (IEX) and size-exclusion chromatography (SEC). IMAC purification was performed on the 5 ml HisTrap FF Crude column (Cytiva) at...

Sf21 cells in Sf-900 II serum-free medium (Gibco, 11497-013) were co-infected at a multiplicity of infection of 1 for both His-tagged METTL1 and Flag-tagged WDR4 baculovirus preparations. The infected cultures were incubated for 72 h at 110 rpm and 27 °C. Cells were collected by centrifugation at 3,400g for 15 min at 4 °C and the pellets were stored at −80 °C. The pellets were lysed in 25 mM Tris pH 8.0 (Invitrogen, 15568025), 300 mM NaCl (Merck, S6546), 1 mM tris(2-carboxyethyl)phosphine (TCEP) (Thermo Fisher Scientific, 20490), 5% glycerol (Thermo Fisher Scientific, 327255000), 0.25% CHAPS (Thermo Fisher Scientific, 28300), 1 mM phenylmethylsulfonyl fluoride (PMSF) (Thermo Fisher Scientific, 36978) supplemented with protease inhibitor tablets (Roche, 4693124001) and 10 U ml−1 benzonase (Merck, E8263). Homogenization was achieved by two cycles at 20 kilopounds per square inch using an IKA high pressure homogenizer. Lysates were briefly centrifuged in between cycles (2,000 rpm for 15 min) to remove foam and finally cleared by ultracentrifugation (45 min at 150,000g, 4 °C). The protein complex was purified sequentially by immobilized metal affinity chromatography (IMAC), desalting, ion-exchange chromatography (IEX) and size-exclusion chromatography (SEC). IMAC purification was performed on the 5 ml HisTrap FF Crude column (Cytiva) at...

The Tris(2-chloroethyl) Phosphate (TCEP) market was valued at USD 1.2 billion in 2025 and is poised to grow from USD 1.

Cell Death Discovery - UVB-induced keratinocyte disulfidptosis drives skin photodamage and photoaging

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Chlorinated organophosphate esters (Cl-OPEs)—specifically tris(2-chloroethyl) phosphate (TCEP), tris(2-chloroisopropyl) phosphate (TCIPP), and tris(1,3-dichloroisopropyl) phosphate (TDCIPP—are added t...

Organophosphate esters (OPEs) are used in furniture, electronics, textiles and building materials. Because they are physically mixed in rather than chemically bound, they leach out continuously into air, dust and water. Once in aquatic environments, they are taken up by algae, ingested by small animals and passed up the food web. Some OPEs are known endocrine disruptors, neurotoxicants and carcinogens. Most research has focused on either marine or freshwater systems separately, leaving a critical gap: no one knew whether a chemical that dilutes in the ocean might magnify in a river – or the reverse. Given these uncertainties, a systematic cross‑ecosystem comparison became essential. A team from Changchun Normal University and the Chinese Academy of Sciences has conducted the first side‑by‑side comparison of OPE bioaccumulation and trophic transfer in contrasting aquatic food webs. The study, published (DOI: 10.1007/s11783-026-2275-9) in the journal ENGINEERING Environment (Volume 20, Issue 11, 2026), analysed 498 organisms representing 34 species collected from Liaodong Bay (marine) and the Songhua River (freshwater) in northeastern China. The researchers found striking differences. In the marine food web, tri‑n‑propyl phosphate (TPP) dominated, with median concentrations of 147 ng/g lipid weight. In the freshwater system, tris(2‑chloroethyl) phosphate (TCEP) was the most abundant,...

Dermal uptake from indoor dust—especially within residential and vehicular environments—constitutes a potentially important exposure route. This study aimed to estimate daily intakes (EDIs) via dermal...

Analytical chemistry · Hu Y, Zhou Y, Yin H.

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