Recent structural, functional and dynamic research on the bacterial melibiose permease MelB has revealed significant insights into the symport mechanisms of Na+-coupled MFS transporters #CryoEM #XRayCrystallography #HDXMS doi.org/10.1107/S205...
October 15, 2026 at 2:10 PM
Recent structural, functional and dynamic research on the bacterial melibiose permease MelB has revealed significant insights into the symport mechanisms of Na+-coupled MFS transporters #CryoEM #XRayCrystallography #HDXMS doi.org/10.1107/S205...
A practical approach for ligand validation during structure determination is outlined and key pitfalls are illustrated by re-examining several deposited PDB entries #LigandValidation #XRayCrystallography #StructureGuidedDrugDiscovery doi.org/10.1107/S205...
October 14, 2026 at 1:01 PM
A practical approach for ligand validation during structure determination is outlined and key pitfalls are illustrated by re-examining several deposited PDB entries #LigandValidation #XRayCrystallography #StructureGuidedDrugDiscovery doi.org/10.1107/S205...
Exploration of the acetyl-pyrrole scaffold led to the identification of BAZ2A-binding compounds assuming a peculiar, almost enclosed, conformation, as determined by X-ray crystallography #BAZ2A #Bromodomains #XRayCrystallography doi.org/10.1107/S205...
September 9, 2026 at 2:12 PM
Exploration of the acetyl-pyrrole scaffold led to the identification of BAZ2A-binding compounds assuming a peculiar, almost enclosed, conformation, as determined by X-ray crystallography #BAZ2A #Bromodomains #XRayCrystallography doi.org/10.1107/S205...
The replacement of DTT with TCEP in the crystallization of human aldehyde oxidase provides a strategy to circumvent enzyme inactivation during crystallographic studies #HumanAldehydeOxidase #XRayCrystallography #TCEP doi.org/10.1107/S205...
August 26, 2026 at 2:25 PM
The replacement of DTT with TCEP in the crystallization of human aldehyde oxidase provides a strategy to circumvent enzyme inactivation during crystallographic studies #HumanAldehydeOxidase #XRayCrystallography #TCEP doi.org/10.1107/S205...
Dorothy Hodgkin, British Biochemist who revolutionized the field of X-ray crystallography! Read: buff.ly/St7N1Ow
Post by Sumbul, Illustration by Miler, Sci-Illustrate Stories. Set in motion by Radhika Patnala.#WomenInScience #WomenInSTEM #NobelPrize #sciart #crystallography #XRayCrystallography
Post by Sumbul, Illustration by Miler, Sci-Illustrate Stories. Set in motion by Radhika Patnala.#WomenInScience #WomenInSTEM #NobelPrize #sciart #crystallography #XRayCrystallography
May 12, 2025 at 6:30 AM
Dorothy Hodgkin, British Biochemist who revolutionized the field of X-ray crystallography! Read: buff.ly/St7N1Ow
Post by Sumbul, Illustration by Miler, Sci-Illustrate Stories. Set in motion by Radhika Patnala.#WomenInScience #WomenInSTEM #NobelPrize #sciart #crystallography #XRayCrystallography
Post by Sumbul, Illustration by Miler, Sci-Illustrate Stories. Set in motion by Radhika Patnala.#WomenInScience #WomenInSTEM #NobelPrize #sciart #crystallography #XRayCrystallography
🧪 The Preclinical Drug Development Facility provides an integrated workflow from gene cloning to high-resolution protein structure determination.
More: inmolcell.iimcb.gov.pl
#IIMCB #INMOLCELL #PreclinicalDrugDevelopment #ProteinScience #StructuralBiology #CryoEM #XrayCrystallography
More: inmolcell.iimcb.gov.pl
#IIMCB #INMOLCELL #PreclinicalDrugDevelopment #ProteinScience #StructuralBiology #CryoEM #XrayCrystallography
July 14, 2026 at 7:50 AM
🧪 The Preclinical Drug Development Facility provides an integrated workflow from gene cloning to high-resolution protein structure determination.
More: inmolcell.iimcb.gov.pl
#IIMCB #INMOLCELL #PreclinicalDrugDevelopment #ProteinScience #StructuralBiology #CryoEM #XrayCrystallography
More: inmolcell.iimcb.gov.pl
#IIMCB #INMOLCELL #PreclinicalDrugDevelopment #ProteinScience #StructuralBiology #CryoEM #XrayCrystallography
A comprehensive overview of how X-ray crystallography, cryo-electron microscopy, micro-electron diffraction and cryo-electron tomography have advanced drug discovery across diverse therapeutic areas is provided #XRayCrystallography #CryoEM #CryoET doi.org/10.1107/S205...
July 23, 2026 at 1:52 PM
A comprehensive overview of how X-ray crystallography, cryo-electron microscopy, micro-electron diffraction and cryo-electron tomography have advanced drug discovery across diverse therapeutic areas is provided #XRayCrystallography #CryoEM #CryoET doi.org/10.1107/S205...
A comparison between the use of electrons and X-rays for collecting diffraction data from small protein crystals and imaging data from cells and tissues #XRayCrystallography #ElectronCrystallography #ElectronImaging doi.org/10.1107/S205...
June 24, 2026 at 2:09 PM
A comparison between the use of electrons and X-rays for collecting diffraction data from small protein crystals and imaging data from cells and tissues #XRayCrystallography #ElectronCrystallography #ElectronImaging doi.org/10.1107/S205...
Two crystallographic fragment-screening campaigns against SARS-CoV-2 NSP1 identified 21 hits from 192 screened fragments, the highest hit rate reported for Nsp1 to date #XRayCrystallography #SARSCoV2 #CrystallographicFragmentScreening doi.org/10.1107/S205...
November 13, 2025 at 3:08 PM
Two crystallographic fragment-screening campaigns against SARS-CoV-2 NSP1 identified 21 hits from 192 screened fragments, the highest hit rate reported for Nsp1 to date #XRayCrystallography #SARSCoV2 #CrystallographicFragmentScreening doi.org/10.1107/S205...
A method is presented to robustly exclude erroneous reflection measurements arising from beamstop shadows in crystallographic diffraction experiments #XRayCrystallography #AUSPEX #Outliers doi.org/10.1107/S205...
October 9, 2024 at 12:38 PM
A method is presented to robustly exclude erroneous reflection measurements arising from beamstop shadows in crystallographic diffraction experiments #XRayCrystallography #AUSPEX #Outliers doi.org/10.1107/S205...
Crystallographic evidence is presented for a putative disulfide molecular switch sensitive to the binding of BIA ligands in the opium poppy latex protein PR10-10 #XRayCrystallography #LigandCoupledConformationalChanges #ProteinStructureAndFunction t.co/JacXTgOW9Q
September 11, 2024 at 3:13 PM
Crystallographic evidence is presented for a putative disulfide molecular switch sensitive to the binding of BIA ligands in the opium poppy latex protein PR10-10 #XRayCrystallography #LigandCoupledConformationalChanges #ProteinStructureAndFunction t.co/JacXTgOW9Q
A structure of carbonic anhydrase II was determined by XFEL serial femtosecond crystallography and is compared with previously reported NMR and synchrotron X-ray and neutron single-crystal structures #CarbonicAnhydraseII #XFEL #XRayCrystallography t.co/7aGMCw9g5j
March 20, 2024 at 1:55 PM
A structure of carbonic anhydrase II was determined by XFEL serial femtosecond crystallography and is compared with previously reported NMR and synchrotron X-ray and neutron single-crystal structures #CarbonicAnhydraseII #XFEL #XRayCrystallography t.co/7aGMCw9g5j
The first structure of a cold-active arginase reveals a modest reduction in structural rigidity associated with cold adaptation #ColdActiveEnzymes #Arginases #XRayCrystallography doi.org/10.1107/S205...
June 10, 2026 at 1:35 PM
The first structure of a cold-active arginase reveals a modest reduction in structural rigidity associated with cold adaptation #ColdActiveEnzymes #Arginases #XRayCrystallography doi.org/10.1107/S205...
Modifying the bacterial strain led to cleaner bacterial expression of a synthetic BRIL antibody that facilitates protein structure determination via both crystallography and cryo-EM #CryoEM #XRayCrystallography #BRIL doi.org/10.1107/S205...
April 23, 2026 at 2:50 PM
Modifying the bacterial strain led to cleaner bacterial expression of a synthetic BRIL antibody that facilitates protein structure determination via both crystallography and cryo-EM #CryoEM #XRayCrystallography #BRIL doi.org/10.1107/S205...
High-resolution structures of α-bCA2 with sulfonamide derivatives provides experimentally supported information defining their binding mode and supporting inhibitor optimization #CarbonicAnhydraseII #SulfonamideInhibitors #XRayCrystallography doi.org/10.1107/S205...
April 22, 2026 at 2:46 PM
High-resolution structures of α-bCA2 with sulfonamide derivatives provides experimentally supported information defining their binding mode and supporting inhibitor optimization #CarbonicAnhydraseII #SulfonamideInhibitors #XRayCrystallography doi.org/10.1107/S205...
Cooper and Isom: Optimized bacterial expression of a synthetic BRIL antibody ##CryoEM##XRayCrystallography##BRIL@UniofOxford@UniofOxford...##IUCrhttps://journals.iucr.org/paper?S2053230X26001548
Optimized bacterial expression of a synthetic BRIL antibody
Here, we document the generation of a T7 Express ΔcybC strain allowing contaminant-free expression of the anti-BRIL Fab BAG2. We also report the crystal structure of BAG2 in complex with native cytochrome b562, a complex arising from expression in canonical Escherichia coli strains.
journals.iucr.org
March 18, 2026 at 1:00 AM
Cooper and Isom: Optimized bacterial expression of a synthetic BRIL antibody ##CryoEM##XRayCrystallography##BRIL@UniofOxford@UniofOxford...##IUCrhttps://journals.iucr.org/paper?S2053230X26001548
The highest resolution DNA-free TaXPD structure to date uniquely resolves loop 510–514 in the long-form, DNA-free TaXPD model #XRayCrystallography #Helicases #Archaea doi.org/10.1107/S205...
February 17, 2026 at 3:16 PM
The highest resolution DNA-free TaXPD structure to date uniquely resolves loop 510–514 in the long-form, DNA-free TaXPD model #XRayCrystallography #Helicases #Archaea doi.org/10.1107/S205...
Features of sample preparation that should be considered prior to biomolecular crystallization experiments are reviewed, as described in the SAMPREP workshop during the 73rd Annual ACA Meeting #Crystallization #XRayCrystallography #StructuralBiology doi.org/10.1107/S205...
July 14, 2025 at 1:29 PM
Features of sample preparation that should be considered prior to biomolecular crystallization experiments are reviewed, as described in the SAMPREP workshop during the 73rd Annual ACA Meeting #Crystallization #XRayCrystallography #StructuralBiology doi.org/10.1107/S205...
The structure of the C1 domain of the surface-layer protein SlpM from L. brevis provides new insights into the architecture and evolutionary adaptability of SLPs in Lactobacillus species #XRayCrystallography #SurfaceLayerProtein #SlpM doi.org/10.1107/S205...
June 24, 2025 at 3:02 PM
The structure of the C1 domain of the surface-layer protein SlpM from L. brevis provides new insights into the architecture and evolutionary adaptability of SLPs in Lactobacillus species #XRayCrystallography #SurfaceLayerProtein #SlpM doi.org/10.1107/S205...
Gabrielle R. Budziszewski et al.: Preparing for successful protein crystallization experiments ##Crystallization##XRayCrystallography##StructuralBiology... ##IUCrhttps://journals.iucr.org/paper?S2053230X25004650
Preparing for successful protein crystallization experiments
All crystal-based structural biology methods, including X-ray crystallography, serial synchrotron and serial femtosecond crystallography, and electron diffraction, require the preparation of biomolecular crystals. This article covers strategies for careful sample preparation in crystallization experiments to increase the chance of success.
journals.iucr.org
June 3, 2025 at 12:00 AM
Gabrielle R. Budziszewski et al.: Preparing for successful protein crystallization experiments ##Crystallization##XRayCrystallography##StructuralBiology... ##IUCrhttps://journals.iucr.org/paper?S2053230X25004650
Yi Xue and Xue Kang: Crystal structure of the C1 domain of the surface-layer protein SlpM from Lactobacillus brevis: a module involved in protein self-assembly ##XRayCrystallography##SurfaceLayerProtein##SlpM... ##IUCrhttps://journals.iucr.org/paper?S2053230X25004194
Crystal structure of the C1 domain of the surface-layer protein SlpM from Lactobacillus brevis: a module involved in protein self-assembly
The 1.7 Å resolution structure of the C1 domain of the surface-layer protein SlpM from L. brevis is reported. The contact surfaces observed in the crystal lattice potentially contribute to the self-assembly process of SlpM.
journals.iucr.org
May 16, 2025 at 12:00 AM
Yi Xue and Xue Kang: Crystal structure of the C1 domain of the surface-layer protein SlpM from Lactobacillus brevis: a module involved in protein self-assembly ##XRayCrystallography##SurfaceLayerProtein##SlpM... ##IUCrhttps://journals.iucr.org/paper?S2053230X25004194
Shin Ando et al.: The first report of structural analysis of a nucleic acid using crystals grown in space. Corrigendum ##XRayCrystallography##NucleicAcids##Microgravity... ##IUCrhttps://journals.iucr.org/paper?S2053230X25002766
The first report of structural analysis of a nucleic acid using crystals grown in space. Corrigendum
The article by Ando et al. [(2025), Acta Cryst. F81, 95–100] is corrected.
journals.iucr.org
March 31, 2025 at 12:00 AM
Shin Ando et al.: The first report of structural analysis of a nucleic acid using crystals grown in space. Corrigendum ##XRayCrystallography##NucleicAcids##Microgravity... ##IUCrhttps://journals.iucr.org/paper?S2053230X25002766
Shin Ando et al.: The first report of structural analysis of a nucleic acid using crystals grown in space ##XRayCrystallography##NucleicAcids##Microgravity... ##IUCrhttps://journals.iucr.org/paper?S2053230X25000810
The first report of structural analysis of a nucleic acid using crystals grown in space
The first structural analysis of a nucleic acid using crystals obtained under microgravity crystallization in space is reported. A DNA/RNA heteroduplex was successfully crystallized in a microgravity environment, improving the size and appearance of the crystals compared with crystals obtained on Earth.
journals.iucr.org
February 13, 2025 at 1:00 AM
Shin Ando et al.: The first report of structural analysis of a nucleic acid using crystals grown in space ##XRayCrystallography##NucleicAcids##Microgravity... ##IUCrhttps://journals.iucr.org/paper?S2053230X25000810
Unlike other serpins, human serpin B9 shows significant structural deviations around helix D, with a larger surface cavity, which could serve as a promising target for small-molecule inhibitors #Serpins #XRayCrystallography #Crystallization t.co/ilB7Aa3Krg
November 11, 2024 at 2:42 PM
Unlike other serpins, human serpin B9 shows significant structural deviations around helix D, with a larger surface cavity, which could serve as a promising target for small-molecule inhibitors #Serpins #XRayCrystallography #Crystallization t.co/ilB7Aa3Krg