Aging clocks are typically trained on pooled multi-tissue data, implicitly assuming that aging is uniform across organs. Here we challenge this assumption by constructing 49 transcriptomic clocks acro...

Dogs with the most common and aggressive form of lymphoma may soon benefit from a clearer molecular map of their disease, thanks to a new transcriptomic study from Brazilian researchers. A team led by

A population-scale single-cell transcriptomic atlas of the human dorsolateral prefrontal cortex provides a perspective of the transcriptomic landscape in neurodegenerative and neuropsychiatric disorders, and offers potential targets for therapeutic intervention.

Publication date: Available online 26 September 2026 Source: Emerging Contaminants Author(s): Dae-Han Park, Ji-Hun Jang, Hyun Bo Sim, Jong-Jin Kim, Seung-Hyun Jeong

Read the full paper: A transcriptomic and spatial map of serotonin autoreceptor expression in Drosophila

Naz et al. benchmark reproducible microglial states across adulthood, aging, and parabiosis to define a conserved activation program and both shared and region-specific transcriptomic remodeling. They further reveal regional differences in microglial sensitivity to parabiosis-mediated accelerated aging and rejuvenation, with the cerebellum the most sensitive and the striatum the least.

A population-scale single-cell transcriptomic atlas of the human dorsolateral prefrontal cortex provides a perspective of the transcriptomic landscape in neurodegenerative and neuropsychiatric disorde...

Single-cell sequencing (SCS) techniques have the potential to offer precise insights into cellular diversity by revealing unique genetic and transcriptomic p...

Elderly human donor microbiota disrupts intestinal barrier integrity and reshapes host metabolic and mucosal transcriptional profiles in human microbiota-associated pigs. Integrated multi-omics analy...

Population-associated molecular variation in breast tissue may contribute to differences in tissue biology and disease susceptibility. Still, the extent to which such variation is shaped by underlying tissue state remains unclear. We performed a pilot RNA-seq and lipidomic analysis of histologically normal breast tissue from African American (AA) and Caucasian White (CW) individuals. Unsupervised transcriptomic analysis identified two baseline tissue states, G1 and G2, representing the dominant...

Understanding genome regulation is limited by the complexity of molecular interactions in living cells. Cell-free systems provide a simplified platform for studying gene expression, but low mRNA levels have prevented RNA-seq. To address this, we develop an active learning workflow combining Bayesian optimization with automated high-throughput experimentation to systematically explore over 1.6 million buffer compositions, experimentally testing 653. We identify a “mRNA-optimized” buffer (20-fold increase in mRNA yield) and a “trade-off” buffer (13-fold increase while maintaining protein production). Using direct RNA-seq, we profile the T7 phage transcriptome in cell-free systems and compare it with a purified T7-RNAP transcription system and phage-infected bacteria. This comparative analysis reveals distinct regulatory layers: the T7-RNAP system captures promoter-strength hierarchies but lacks RNA degradation, whereas cell-free systems provide an accurate estimation of in vivo expressio