Happy to share our latest publication on the role of Citrullination in the hair follicle stem cell lineage! Congratulations Kim Vikhe Patil and our fantastic collaborators from the Maria Kasper, Ryan Driskell and Priyanka Sharma labs!
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
The citrullinating enzyme PADI4 governs progenitor cell proliferation and translation in developing hair follicles
Citrullination, a posttranslational modification, affects progenitor cell states and tissue formation.
www.science.org
September 13, 2025 at 7:06 AM
Happy to share our latest publication on the role of Citrullination in the hair follicle stem cell lineage! Congratulations Kim Vikhe Patil and our fantastic collaborators from the Maria Kasper, Ryan Driskell and Priyanka Sharma labs!
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
High-throughput chemical proteomics workflow for profiling protein citrullination dynamics www.nature.com/artic...
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#proteomics #prot-paper
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#proteomics #prot-paper
February 22, 2026 at 5:00 PM
High-throughput chemical proteomics workflow for profiling protein citrullination dynamics www.nature.com/artic...
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#proteomics #prot-paper
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#proteomics #prot-paper
Our proteomics workflow for profiling citrullination dynamics is out 🎉
www.nature.com/articles/s41...
Idea from a journal club discussion + Funding + Determined PhD student + many “this didn’t work…” moments =
A robust, accessible citrullinome enrichment workflow
#proteomics #citrullination #PTM
www.nature.com/articles/s41...
Idea from a journal club discussion + Funding + Determined PhD student + many “this didn’t work…” moments =
A robust, accessible citrullinome enrichment workflow
#proteomics #citrullination #PTM
High-throughput chemical proteomics workflow for profiling protein citrullination dynamics - Nature Communications
Protein citrullination is linked to autoimmunity and inflammation but is challenging to detect globally. Here, the authors report a high-throughput chemical proteomics workflow and demonstrate its uti...
www.nature.com
February 23, 2026 at 3:19 PM
Our proteomics workflow for profiling citrullination dynamics is out 🎉
www.nature.com/articles/s41...
Idea from a journal club discussion + Funding + Determined PhD student + many “this didn’t work…” moments =
A robust, accessible citrullinome enrichment workflow
#proteomics #citrullination #PTM
www.nature.com/articles/s41...
Idea from a journal club discussion + Funding + Determined PhD student + many “this didn’t work…” moments =
A robust, accessible citrullinome enrichment workflow
#proteomics #citrullination #PTM
Our first #MicroscopyMonday of 2025 comes with a bonus blog! All thanks to @mariachristophorou.bsky.social 's lab
📝by PhD student Noah - Secrets no one has told you about histones www.babraham.ac.uk/blog/histone...
📸 of extracellular histones marked by citrullination by Marie Curie fellow Johanna
📝by PhD student Noah - Secrets no one has told you about histones www.babraham.ac.uk/blog/histone...
📸 of extracellular histones marked by citrullination by Marie Curie fellow Johanna
January 13, 2025 at 3:01 PM
Our first #MicroscopyMonday of 2025 comes with a bonus blog! All thanks to @mariachristophorou.bsky.social 's lab
📝by PhD student Noah - Secrets no one has told you about histones www.babraham.ac.uk/blog/histone...
📸 of extracellular histones marked by citrullination by Marie Curie fellow Johanna
📝by PhD student Noah - Secrets no one has told you about histones www.babraham.ac.uk/blog/histone...
📸 of extracellular histones marked by citrullination by Marie Curie fellow Johanna
Kicking off the new year strong! Prosit-Cit is officially online!🍻 Check it out: www.mcponline.org/article/S153...
#DeepLearning #Citrullination #Bioinformatics
#DeepLearning #Citrullination #Bioinformatics
Deep Learning Enhances Precision of Citrullination Identification in Human and Plant Tissue Proteomes
Citrullination is a critical yet understudied post-translational modification (PTM)
implicated in various biological processes. Exploring its role in health and disease
requires a comprehensive unders...
www.mcponline.org
February 6, 2025 at 5:36 AM
Kicking off the new year strong! Prosit-Cit is officially online!🍻 Check it out: www.mcponline.org/article/S153...
#DeepLearning #Citrullination #Bioinformatics
#DeepLearning #Citrullination #Bioinformatics
#AlzheimersDisease
Quantitative👤🧠Amyloidβ proteoforms (N-/C-terminal isoforms + post-translational modifications - Asp1/7 isomerization, pGlu3 pyroglutamate, Arg5 citrullination) in vessel vs parenchyma
20 AD (4-lv #CerebralAmyloidAngiopathy) vs 5 Ctrl
bioRxiv 2025
www.biorxiv.org/content/10.1...
Quantitative👤🧠Amyloidβ proteoforms (N-/C-terminal isoforms + post-translational modifications - Asp1/7 isomerization, pGlu3 pyroglutamate, Arg5 citrullination) in vessel vs parenchyma
20 AD (4-lv #CerebralAmyloidAngiopathy) vs 5 Ctrl
bioRxiv 2025
www.biorxiv.org/content/10.1...
July 15, 2025 at 11:57 AM
#AlzheimersDisease
Quantitative👤🧠Amyloidβ proteoforms (N-/C-terminal isoforms + post-translational modifications - Asp1/7 isomerization, pGlu3 pyroglutamate, Arg5 citrullination) in vessel vs parenchyma
20 AD (4-lv #CerebralAmyloidAngiopathy) vs 5 Ctrl
bioRxiv 2025
www.biorxiv.org/content/10.1...
Quantitative👤🧠Amyloidβ proteoforms (N-/C-terminal isoforms + post-translational modifications - Asp1/7 isomerization, pGlu3 pyroglutamate, Arg5 citrullination) in vessel vs parenchyma
20 AD (4-lv #CerebralAmyloidAngiopathy) vs 5 Ctrl
bioRxiv 2025
www.biorxiv.org/content/10.1...
Cellular Consequences, Citrullination Substrates, and Antigenicity of Targeting PAD4 to Cell Surfaces | ACS Central Science
doi.org/10.1021/acsc...
doi.org/10.1021/acsc...
Cellular Consequences,
Citrullination Substrates,
and Antigenicity of Targeting PAD4 to Cell Surfaces
Abstract. Protein arginine deiminase-4 (PAD4) catalyzes hydrolysis
of arginine
to citrulline in proteins that promote widespread cellular changes
that can
doi.org
July 25, 2026 at 5:31 PM
Cellular Consequences, Citrullination Substrates, and Antigenicity of Targeting PAD4 to Cell Surfaces | ACS Central Science
doi.org/10.1021/acsc...
doi.org/10.1021/acsc...
Not the most obvious project for a mass spec lab...but here we are, making antibodies.😀
Our new paper reports two motif-based mAbs that broadly detect citrullination, distinguish it from homocitrullination, and work in ELISA, WB, and IF. www.cell.com/cell-reports...
#citrullination #antibodies #PTM
Our new paper reports two motif-based mAbs that broadly detect citrullination, distinguish it from homocitrullination, and work in ELISA, WB, and IF. www.cell.com/cell-reports...
#citrullination #antibodies #PTM
Development of motif-specific monoclonal antibodies for global protein citrullination detection with minimal cross-reactivity to homocitrullination
Laposchan et al. report a motif-based strategy to generate monoclonal antibodies for
global detection of protein citrullination. The antibodies show strong sensitivity
and selectivity over homocitrull...
www.cell.com
March 30, 2026 at 1:19 PM
Not the most obvious project for a mass spec lab...but here we are, making antibodies.😀
Our new paper reports two motif-based mAbs that broadly detect citrullination, distinguish it from homocitrullination, and work in ELISA, WB, and IF. www.cell.com/cell-reports...
#citrullination #antibodies #PTM
Our new paper reports two motif-based mAbs that broadly detect citrullination, distinguish it from homocitrullination, and work in ELISA, WB, and IF. www.cell.com/cell-reports...
#citrullination #antibodies #PTM
Because science camaraderie is forever: New work on citrullination from the @genanderlab.bsky.social and my good friend Kim! This hits close to home as we had a lot of discussions with Kim about protein translation in development and OPP assays during our PhD time! www.science.org/doi/abs/10.1...
The citrullinating enzyme PADI4 governs progenitor cell proliferation and translation in developing hair follicles
Citrullination, a posttranslational modification, affects progenitor cell states and tissue formation.
www.science.org
September 13, 2025 at 6:28 AM
Because science camaraderie is forever: New work on citrullination from the @genanderlab.bsky.social and my good friend Kim! This hits close to home as we had a lot of discussions with Kim about protein translation in development and OPP assays during our PhD time! www.science.org/doi/abs/10.1...
A new histone modification, citrullination, prevents HIV latency and stimulates proviral transcription, as shown by our latest publication @ki.se @natcomms.nature.com rdcu.be/eu68q
PADI4-mediated citrullination of histone H3 stimulates HIV-1 transcription
Nature Communications - Upon infection with HIV-1 and proviral integration, the host enzyme PADI4 citrullinates histone H3 at the integration site, leading to non-repressed HIV-1 transcription....
rdcu.be
July 7, 2025 at 10:15 AM
A new histone modification, citrullination, prevents HIV latency and stimulates proviral transcription, as shown by our latest publication @ki.se @natcomms.nature.com rdcu.be/eu68q
Citrullination in tumor immunity and therapy
This Review by Michael R. Pitter & Weiping Zou discusses how citrullination regulates immune cell function within the tumor microenvironment and its potential as a target in cancer immunotherapy: www.jci.org/articles/vie...
This Review by Michael R. Pitter & Weiping Zou discusses how citrullination regulates immune cell function within the tumor microenvironment and its potential as a target in cancer immunotherapy: www.jci.org/articles/vie...
October 16, 2025 at 4:15 PM
Citrullination in tumor immunity and therapy
This Review by Michael R. Pitter & Weiping Zou discusses how citrullination regulates immune cell function within the tumor microenvironment and its potential as a target in cancer immunotherapy: www.jci.org/articles/vie...
This Review by Michael R. Pitter & Weiping Zou discusses how citrullination regulates immune cell function within the tumor microenvironment and its potential as a target in cancer immunotherapy: www.jci.org/articles/vie...
Great start of our #ChemBio workshop with a fantastic talk from @mariachristophorou.bsky.social from @babrahaminst.bsky.social on citrullination modulators.
February 14, 2025 at 1:32 PM
Great start of our #ChemBio workshop with a fantastic talk from @mariachristophorou.bsky.social from @babrahaminst.bsky.social on citrullination modulators.
Astrocytic PAD2-mediated citrullination of vimentin activates microglia, worsens Aβ accumulation, & exacerbates cognitive deficits, highlighting astrocyte-microglia x-talk as therapeutic target for Alzheimer’s @cp-immunity.bsky.social
www.cell.com/immunity/ful...
www.cell.com/immunity/ful...
www.cell.com/immunity/ful...
www.cell.com/immunity/ful...
June 9, 2026 at 3:46 PM
Astrocytic PAD2-mediated citrullination of vimentin activates microglia, worsens Aβ accumulation, & exacerbates cognitive deficits, highlighting astrocyte-microglia x-talk as therapeutic target for Alzheimer’s @cp-immunity.bsky.social
www.cell.com/immunity/ful...
www.cell.com/immunity/ful...
www.cell.com/immunity/ful...
www.cell.com/immunity/ful...
Hello Baltimore! We're looking forward to #ASMS2025 and all the new developments in mass-spec and proteomics.
If you're attending today, make sure to check out @msleelab.bsky.social's poster on citrullination in multiple sclerosis. Sophia 'Lapo' Laposchan is excited to see you there!
If you're attending today, make sure to check out @msleelab.bsky.social's poster on citrullination in multiple sclerosis. Sophia 'Lapo' Laposchan is excited to see you there!
June 2, 2025 at 8:14 AM
Hello Baltimore! We're looking forward to #ASMS2025 and all the new developments in mass-spec and proteomics.
If you're attending today, make sure to check out @msleelab.bsky.social's poster on citrullination in multiple sclerosis. Sophia 'Lapo' Laposchan is excited to see you there!
If you're attending today, make sure to check out @msleelab.bsky.social's poster on citrullination in multiple sclerosis. Sophia 'Lapo' Laposchan is excited to see you there!
Many congratulations Chien-Yun and team! Great to see this out. Prosit-Cit is an important addition to the the community’s toolkit for studying #citrullination
Kicking off the new year strong! Prosit-Cit is officially online!🍻 Check it out: www.mcponline.org/article/S153...
#DeepLearning #Citrullination #Bioinformatics
#DeepLearning #Citrullination #Bioinformatics
Deep Learning Enhances Precision of Citrullination Identification in Human and Plant Tissue Proteomes
Citrullination is a critical yet understudied post-translational modification (PTM)
implicated in various biological processes. Exploring its role in health and disease
requires a comprehensive unders...
www.mcponline.org
February 6, 2025 at 6:43 AM
Many congratulations Chien-Yun and team! Great to see this out. Prosit-Cit is an important addition to the the community’s toolkit for studying #citrullination
New preprint! 🎉
www.biorxiv.org/content/10.1...
If you've ever tried detecting citrullinated proteins, you know the pain—most pan-citrullination antibodies are either useless, cross-reactive, or heartbreakingly bad. 💔😭
I tested them. I suffered. I cried. So we made our own.
www.biorxiv.org/content/10.1...
If you've ever tried detecting citrullinated proteins, you know the pain—most pan-citrullination antibodies are either useless, cross-reactive, or heartbreakingly bad. 💔😭
I tested them. I suffered. I cried. So we made our own.
Development of Motif-Specific Monoclonal Antibodies for Global Protein Citrullination Detection with Minimal Cross-Reactivity to Homocitrullination
Protein citrullination, a post-translational modification (PTM) catalyzed by peptidylarginine deiminases (PADs), plays critical roles in biological processes such as immunity, gene regulation, and inf...
www.biorxiv.org
March 31, 2025 at 9:30 AM
New preprint! 🎉
www.biorxiv.org/content/10.1...
If you've ever tried detecting citrullinated proteins, you know the pain—most pan-citrullination antibodies are either useless, cross-reactive, or heartbreakingly bad. 💔😭
I tested them. I suffered. I cried. So we made our own.
www.biorxiv.org/content/10.1...
If you've ever tried detecting citrullinated proteins, you know the pain—most pan-citrullination antibodies are either useless, cross-reactive, or heartbreakingly bad. 💔😭
I tested them. I suffered. I cried. So we made our own.
PADI4 citrullinates p53, redirecting its binding to ETS sites, enhancing immune-related gene expression, and promoting tumor suppression in @cp-molcell.bsky.social
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
Targeted citrullination enables p53 binding to non-canonical sites
Indeglia et al. describe a new post-translational modification that alters p53 function.
www.cell.com
October 3, 2025 at 7:42 AM
PADI4 citrullinates p53, redirecting its binding to ETS sites, enhancing immune-related gene expression, and promoting tumor suppression in @cp-molcell.bsky.social
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
Interesting paper by the group of Michael Nielsen in Nature Chemical Biology. They studied the proteome-wide occurance of citrullination in response to neutrophil differentiation, PADI4 inhibition and increased cellular calcium identifying >14,000 citrullination sites. doi.org/10.1038/s415...
A quantitative and site-specific atlas of the citrullinome reveals widespread existence of citrullination and insights into PADI4 substrates - Nature Structural & Molecular Biology
Here the authors leverage state-of-the-art quantitative proteomics to provide a comprehensive overview of the human citrullinome. Supporting evidence from peptide microarrays hints at the potential cl...
doi.org
March 16, 2024 at 3:22 PM
Interesting paper by the group of Michael Nielsen in Nature Chemical Biology. They studied the proteome-wide occurance of citrullination in response to neutrophil differentiation, PADI4 inhibition and increased cellular calcium identifying >14,000 citrullination sites. doi.org/10.1038/s415...
What a great team this was here at #PfizerChemistry! Discovery of an allosteric PAD2/4 inhibitor that kicks out an embedded water and opens up additional binding pocket – fun synthetic project this one was #ACSMedChemLett asap #ChemSky
Peptidylarginine Deiminase (PAD) Inhibitor Optimization through Displacement of a Trapped Water Molecule
Excess protein citrullination, a post-translational modification converting arginine to citrulline, has been associated with a range of autoimmune and neurological disorders, as well as cancers. Prote...
pubs.acs.org
July 9, 2025 at 9:20 PM
What a great team this was here at #PfizerChemistry! Discovery of an allosteric PAD2/4 inhibitor that kicks out an embedded water and opens up additional binding pocket – fun synthetic project this one was #ACSMedChemLett asap #ChemSky
🔬 The next BayBioMS Advanced Mass Spectrometry Seminar is happening this Wednesday! 🧪
Doors open at 4:45 PM — free drinks and snacks will be served!
This time, we will highlight cutting-edge work in:
#Phosphoproteomics
(Targeted) #peptidomics
#PTM analysis (#citrullination)
Doors open at 4:45 PM — free drinks and snacks will be served!
This time, we will highlight cutting-edge work in:
#Phosphoproteomics
(Targeted) #peptidomics
#PTM analysis (#citrullination)
July 1, 2025 at 4:38 AM
🔬 The next BayBioMS Advanced Mass Spectrometry Seminar is happening this Wednesday! 🧪
Doors open at 4:45 PM — free drinks and snacks will be served!
This time, we will highlight cutting-edge work in:
#Phosphoproteomics
(Targeted) #peptidomics
#PTM analysis (#citrullination)
Doors open at 4:45 PM — free drinks and snacks will be served!
This time, we will highlight cutting-edge work in:
#Phosphoproteomics
(Targeted) #peptidomics
#PTM analysis (#citrullination)
Research Highlight ✨| Targeting anti-PAD4 autoantibodies in RA
Read the original article here: bit.ly/3EVOLpU
@taejoonw.bsky.social
Read the original article here: bit.ly/3EVOLpU
@taejoonw.bsky.social
May 6, 2025 at 2:19 PM
Research Highlight ✨| Targeting anti-PAD4 autoantibodies in RA
Read the original article here: bit.ly/3EVOLpU
@taejoonw.bsky.social
Read the original article here: bit.ly/3EVOLpU
@taejoonw.bsky.social
Don't miss this review in our latest issue from @kanvah.bsky.social et al. at IIT Gandhinagar highlighting the evolution of chemical biology approaches for detecting and profiling protein citrullination #orgchem #chembio
Check it out on our website👇
doi.org/10.1039/d6ob...
Check it out on our website👇
doi.org/10.1039/d6ob...
September 28, 2026 at 9:01 AM
Don't miss this review in our latest issue from @kanvah.bsky.social et al. at IIT Gandhinagar highlighting the evolution of chemical biology approaches for detecting and profiling protein citrullination #orgchem #chembio
Check it out on our website👇
doi.org/10.1039/d6ob...
Check it out on our website👇
doi.org/10.1039/d6ob...
Happy to share our newest preprint as we ring in the new year!
Interested in the cell surface, challenging PTMs, and/or cancer biomarkers? This one has a bit of it all.
"Cellular consequences, citrullination substrates, and antigenicity resulting from wild-type and targeted PAD4 on cell surfaces"
Interested in the cell surface, challenging PTMs, and/or cancer biomarkers? This one has a bit of it all.
"Cellular consequences, citrullination substrates, and antigenicity resulting from wild-type and targeted PAD4 on cell surfaces"
Cellular consequences, citrullination substrates, and antigenicity resulting from wild-type and targeted PAD4 on cell surfaces
Protein arginine deiminase-4 (PAD4) catalyzes hydrolysis of arginine to citrulline in proteins that promotes widespread changes in cellular phenotypes through transcriptional regulation that can induc...
www.biorxiv.org
January 6, 2026 at 10:46 PM
Happy to share our newest preprint as we ring in the new year!
Interested in the cell surface, challenging PTMs, and/or cancer biomarkers? This one has a bit of it all.
"Cellular consequences, citrullination substrates, and antigenicity resulting from wild-type and targeted PAD4 on cell surfaces"
Interested in the cell surface, challenging PTMs, and/or cancer biomarkers? This one has a bit of it all.
"Cellular consequences, citrullination substrates, and antigenicity resulting from wild-type and targeted PAD4 on cell surfaces"
Differential histone tail citrullination by PAD Enzymes observed via NMR spectroscopy https://www.biorxiv.org/content/10.64898/2026.05.01.722238v1
May 6, 2026 at 3:47 AM
Differential histone tail citrullination by PAD Enzymes observed via NMR spectroscopy https://www.biorxiv.org/content/10.64898/2026.05.01.722238v1