Nature research paper: Cryo-EM structure of a methanogen nitrogenase–PII protein supercomplex
go.nature.com/4AWUljR
go.nature.com/4AWUljR
Cryo-EM structure of a methanogen nitrogenase–PII protein supercomplex - Nature
Cryo-electron microscopy of a nitrogenase supercomplex from a methane-producing archaeon reveals a mechanism in which PII proteins drive assembly of such structures to regulate nitrogenase activity in response to nitrogen availability.
go.nature.com
October 13, 2026 at 5:16 PM
Nature research paper: Cryo-EM structure of a methanogen nitrogenase–PII protein supercomplex
go.nature.com/4AWUljR
go.nature.com/4AWUljR
For colleagues in Paris: mini cryo-EM & IA seminar announcement!
On the 23rd of October 11am, in @pasteur.fr , we welcome the great Frosina Stojanovska (Zuse Institute, Berlin) for a talk on **CryoSiam**
From Simulations to Cells: Learning Transferable Representations for Cryo-Electron Tomography
On the 23rd of October 11am, in @pasteur.fr , we welcome the great Frosina Stojanovska (Zuse Institute, Berlin) for a talk on **CryoSiam**
From Simulations to Cells: Learning Transferable Representations for Cryo-Electron Tomography
Seminar [Cryo-ET & AI] – From Simulations to Cells: Learning Transferable Representations for Cryo-Electron Tomography, by Frosina Stojanovska - Research
Abstract : Cryo-electron tomography (cryo-ET) enables the three-dimensional visualization of macromolecular structures directly inside cells, but turning these complex and noisy volumes into biologica...
research.pasteur.fr
October 13, 2026 at 5:01 PM
For colleagues in Paris: mini cryo-EM & IA seminar announcement!
On the 23rd of October 11am, in @pasteur.fr , we welcome the great Frosina Stojanovska (Zuse Institute, Berlin) for a talk on **CryoSiam**
From Simulations to Cells: Learning Transferable Representations for Cryo-Electron Tomography
On the 23rd of October 11am, in @pasteur.fr , we welcome the great Frosina Stojanovska (Zuse Institute, Berlin) for a talk on **CryoSiam**
From Simulations to Cells: Learning Transferable Representations for Cryo-Electron Tomography
1/5 Cryo-EM gives high-resolution RNA structures, but only when high Mg²⁺ freezes out their motion.
At physiological conditions, RNAs are dynamic and 3D reconstruction fails. Can we still learn their folding equilibria?
New preprint: yes, straight from the raw particle images👇
At physiological conditions, RNAs are dynamic and 3D reconstruction fails. Can we still learn their folding equilibria?
New preprint: yes, straight from the raw particle images👇
October 14, 2026 at 4:00 PM
1/5 Cryo-EM gives high-resolution RNA structures, but only when high Mg²⁺ freezes out their motion.
At physiological conditions, RNAs are dynamic and 3D reconstruction fails. Can we still learn their folding equilibria?
New preprint: yes, straight from the raw particle images👇
At physiological conditions, RNAs are dynamic and 3D reconstruction fails. Can we still learn their folding equilibria?
New preprint: yes, straight from the raw particle images👇
A learning-to-rank framework for large-scale identification of unknown proteins from cryo-EM and cryo-ET maps https://www.biorxiv.org/content/10.64898/2026.10.12.758677v1
October 13, 2026 at 8:47 PM
A learning-to-rank framework for large-scale identification of unknown proteins from cryo-EM and cryo-ET maps https://www.biorxiv.org/content/10.64898/2026.10.12.758677v1
A learning-to-rank framework for large-scale identification of unknown proteins from cryo-EM and cryo-ET maps www.biorxiv.org/content/10.64898/2026.10.12.758677v1 #cryoem
October 14, 2026 at 10:27 AM
A learning-to-rank framework for large-scale identification of unknown proteins from cryo-EM and cryo-ET maps www.biorxiv.org/content/10.64898/2026.10.12.758677v1 #cryoem
Check the preprint of our exciting collaboration with
@pilarcossio.bsky.social and others: Quantifying RNA Folding Equilibria from Unclassfied Cryo-EM Images biorxiv.org/content/10.6... see Pilar's very nice description. Congrats Deb Maji and team!
@pilarcossio.bsky.social and others: Quantifying RNA Folding Equilibria from Unclassfied Cryo-EM Images biorxiv.org/content/10.6... see Pilar's very nice description. Congrats Deb Maji and team!
October 14, 2026 at 4:05 PM
Check the preprint of our exciting collaboration with
@pilarcossio.bsky.social and others: Quantifying RNA Folding Equilibria from Unclassfied Cryo-EM Images biorxiv.org/content/10.6... see Pilar's very nice description. Congrats Deb Maji and team!
@pilarcossio.bsky.social and others: Quantifying RNA Folding Equilibria from Unclassfied Cryo-EM Images biorxiv.org/content/10.6... see Pilar's very nice description. Congrats Deb Maji and team!
I wasn't aware of this one, but the AlphaFold team at DeepMind made something similar in 2021:
arxiv.org/abs/2106.14108
arxiv.org/abs/2106.14108
Inferring a Continuous Distribution of Atom Coordinates from Cryo-EM Images using VAEs
Cryo-electron microscopy (cryo-EM) has revolutionized experimental protein structure determination. Despite advances in high resolution reconstruction, a majority of cryo-EM experiments provide either...
arxiv.org
October 10, 2026 at 1:06 PM
I wasn't aware of this one, but the AlphaFold team at DeepMind made something similar in 2021:
arxiv.org/abs/2106.14108
arxiv.org/abs/2106.14108
📖 “Cryo-EM Foundations: Image Formation” takes a step back to look at what happens before data processing begins: the physical processes involved in generating and detecting a cryo-EM image.
October 14, 2026 at 12:00 PM
📖 “Cryo-EM Foundations: Image Formation” takes a step back to look at what happens before data processing begins: the physical processes involved in generating and detecting a cryo-EM image.
CryoSHIELDs: tunable amphipathic peptide additives for cryo-EM www.biorxiv.org/content/10.64898/2026.10.01.755993v1 #cryoem
October 9, 2026 at 10:18 AM
CryoSHIELDs: tunable amphipathic peptide additives for cryo-EM www.biorxiv.org/content/10.64898/2026.10.01.755993v1 #cryoem
Connecting image formation to data processing provides valuable context for interpreting your data, identifying potential artifacts, and understanding the principles behind downstream processing. All this and more, in the #CryoSPARC Guide 👉 guide.cryosparc.com/cryo-em-foun...
Image Formation | CryoSPARC Guide
guide.cryosparc.com
October 14, 2026 at 12:01 PM
Connecting image formation to data processing provides valuable context for interpreting your data, identifying potential artifacts, and understanding the principles behind downstream processing. All this and more, in the #CryoSPARC Guide 👉 guide.cryosparc.com/cryo-em-foun...
Using cryo-EM, we showed that RNF213 forms a zinc-dependent 1.2 MDa dimer, occluding the E2 binding site and catalytic RZ domain to keep the E3 ligase inactive.
October 12, 2026 at 9:34 AM
Using cryo-EM, we showed that RNF213 forms a zinc-dependent 1.2 MDa dimer, occluding the E2 binding site and catalytic RZ domain to keep the E3 ligase inactive.
Purification-free cryo-EM reveals endogenous assemblies from minute tardigrade samples www.biorxiv.org/content/10.64898/2026.10.08.757488v1 #cryoem
October 10, 2026 at 7:10 AM
Purification-free cryo-EM reveals endogenous assemblies from minute tardigrade samples www.biorxiv.org/content/10.64898/2026.10.08.757488v1 #cryoem
Reaction-based cryo-EM resolves the continuous conformational spectrum of CTP synthase catalysis pubmed.ncbi.nlm.nih.gov/42853686/ #cryoem
October 10, 2026 at 7:26 PM
Reaction-based cryo-EM resolves the continuous conformational spectrum of CTP synthase catalysis pubmed.ncbi.nlm.nih.gov/42853686/ #cryoem
5/5 A dream collaboration 🎉🥳, with theory and experiment hand in hand 🙌 with Deb Maji on the experiments,
@steve-bonilla.bsky.social , Alex de Marco @rocovino.bsky.social labs,
@larsdingeldein.bsky.social @minhuanli.bsky.social computational front. Check it out: www.biorxiv.org/content/10.6...
@steve-bonilla.bsky.social , Alex de Marco @rocovino.bsky.social labs,
@larsdingeldein.bsky.social @minhuanli.bsky.social computational front. Check it out: www.biorxiv.org/content/10.6...
Quantifying RNA Folding Equilibria from Unclassified Cryo-EM Particle Images
Functional RNA structures are typically far more dynamic than most proteins solved by cryo-electron microscopy (cryo-EM). Flexible junctions and weak tertiary contacts produce broad conformational ens...
www.biorxiv.org
October 14, 2026 at 4:00 PM
5/5 A dream collaboration 🎉🥳, with theory and experiment hand in hand 🙌 with Deb Maji on the experiments,
@steve-bonilla.bsky.social , Alex de Marco @rocovino.bsky.social labs,
@larsdingeldein.bsky.social @minhuanli.bsky.social computational front. Check it out: www.biorxiv.org/content/10.6...
@steve-bonilla.bsky.social , Alex de Marco @rocovino.bsky.social labs,
@larsdingeldein.bsky.social @minhuanli.bsky.social computational front. Check it out: www.biorxiv.org/content/10.6...
Do you have unmet CryoEM needs? Do you dream of beautiful rotating protein structures. UNC's CryoEM core could be the solution to your problems! www.med.unc.edu/cryo-em/ Contact Josh Strauss joshua_strauss@med.unc.edu to hear about the cutting edge resources availble here! Please share!
October 14, 2026 at 1:32 PM
Do you have unmet CryoEM needs? Do you dream of beautiful rotating protein structures. UNC's CryoEM core could be the solution to your problems! www.med.unc.edu/cryo-em/ Contact Josh Strauss joshua_strauss@med.unc.edu to hear about the cutting edge resources availble here! Please share!
Cryo-tides, and now cryoSHIELDs. Maybe we need to get a peptide synthesiser to the EM facility?
Our latest development is now on bioRxiv: cryoSHIELDs — amphipathic peptide additives that make cryo-EM grid prep far more reliable. Tested across 24 protein systems, they enabled the first ABCC6 structure and GPCR–G protein structures at just 0.1–0.5 mg/mL!
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
October 9, 2026 at 7:55 AM
Cryo-tides, and now cryoSHIELDs. Maybe we need to get a peptide synthesiser to the EM facility?
CryoFAST: A Fab-Aided Cryo-EM Workflow for High-Resolution Structure Determination of Sub-50-kDa Protein Targets www.biorxiv.org/content/10.64898/2026.10.07.757472v1 #cryoem
October 10, 2026 at 1:18 PM
CryoFAST: A Fab-Aided Cryo-EM Workflow for High-Resolution Structure Determination of Sub-50-kDa Protein Targets www.biorxiv.org/content/10.64898/2026.10.07.757472v1 #cryoem
Structural thermodynamics by cryo-EM resolves minute energy differences in heterogeneous protein complexes. www.biorxiv.org/content/10.64898/2026.10.07.756995v1 #cryoem
October 10, 2026 at 11:46 AM
Structural thermodynamics by cryo-EM resolves minute energy differences in heterogeneous protein complexes. www.biorxiv.org/content/10.64898/2026.10.07.756995v1 #cryoem
3/11
Our cryo-EM structure reveals RcaT encased as an inactive dimer within a four-component core retron complex that can further assemble into filaments.
Our cryo-EM structure reveals RcaT encased as an inactive dimer within a four-component core retron complex that can further assemble into filaments.
October 14, 2026 at 1:30 PM
3/11
Our cryo-EM structure reveals RcaT encased as an inactive dimer within a four-component core retron complex that can further assemble into filaments.
Our cryo-EM structure reveals RcaT encased as an inactive dimer within a four-component core retron complex that can further assemble into filaments.
RRIDs were included in this in Science Advances paper. Thanks for making your methods matter! #ReproducibleResearch #OpenResearch #BetterScience
Atomic structure and dynamics of the mechanosensitive channel MscL from Escherichia coli by cryo-EM and solid-state NMR
Read the full paper: Atomic structure and dynamics of the mechanosensitive channel MscL from Escherichia coli by cryo-EM and solid-state NMR
doi.org
October 13, 2026 at 7:00 AM
RRIDs were included in this in Science Advances paper. Thanks for making your methods matter! #ReproducibleResearch #OpenResearch #BetterScience
2/5 Our idea: reweight computational RNA ensembles directly against individual, unclassified cryo-EM images, using probabilistic modeling and deep learning. No averaging, so the heterogeneity isn’t thrown away.
October 14, 2026 at 4:00 PM
2/5 Our idea: reweight computational RNA ensembles directly against individual, unclassified cryo-EM images, using probabilistic modeling and deep learning. No averaging, so the heterogeneity isn’t thrown away.
CryoSHIELDs: tunable amphipathic peptide additives for cryo-EM https://www.biorxiv.org/content/10.64898/2026.10.01.755993v1
October 8, 2026 at 4:51 PM
CryoSHIELDs: tunable amphipathic peptide additives for cryo-EM https://www.biorxiv.org/content/10.64898/2026.10.01.755993v1
Need to ship your #crystallography or #cryoEM samples at cryogenic temperatures? The CX100 is design specifically for that task when combined with our shipping canes, Unipucks or Cryo-EM pucks. Several shipping cases are available as well - Learn More zurl.co/PuVnj
October 12, 2026 at 3:00 PM
Need to ship your #crystallography or #cryoEM samples at cryogenic temperatures? The CX100 is design specifically for that task when combined with our shipping canes, Unipucks or Cryo-EM pucks. Several shipping cases are available as well - Learn More zurl.co/PuVnj
We found that some of these mutations rearrange RNF213 to prevent dimer-mediated inhibition. Cryo-EM, biochemical assays and cellular investigation revealed that some mutants can even achieve the active conformation in the absence of ATP, resulting in increased inflammatory signaling.
October 12, 2026 at 9:34 AM
We found that some of these mutations rearrange RNF213 to prevent dimer-mediated inhibition. Cryo-EM, biochemical assays and cellular investigation revealed that some mutants can even achieve the active conformation in the absence of ATP, resulting in increased inflammatory signaling.
🔎 From contrast in cryo-EM to waves, vectors, and aliasing, these chapters explores key concepts that shape what information is captured, and how it appears in the images we work with.
October 14, 2026 at 12:01 PM
🔎 From contrast in cryo-EM to waves, vectors, and aliasing, these chapters explores key concepts that shape what information is captured, and how it appears in the images we work with.