This is the most detailed 3D rendering of a eukaryotic cell to date, obtained using X-ray, nuclear magnetic resonance (NMR), and cryo-electron microscopy datasets. 🔬🧪
April 29, 2026 at 4:00 PM
This is the most detailed 3D rendering of a eukaryotic cell to date, obtained using X-ray, nuclear magnetic resonance (NMR), and cryo-electron microscopy datasets. 🔬🧪
Applications and prospects of cryo-electron tomography in drug discovery and understanding disease
Review article by @camilaclemente.bsky.social in our lab
www.sciencedirect.com/science/arti...
Review article by @camilaclemente.bsky.social in our lab
www.sciencedirect.com/science/arti...
Applications and prospects of cryo-electron tomography in drug discovery and understanding disease
Cryo-electron tomography (cryo-ET) is emerging as a transformative tool for structural biology. Unlike methods based on purified molecules, cryo-ET en…
www.sciencedirect.com
May 15, 2026 at 8:48 AM
Applications and prospects of cryo-electron tomography in drug discovery and understanding disease
Review article by @camilaclemente.bsky.social in our lab
www.sciencedirect.com/science/arti...
Review article by @camilaclemente.bsky.social in our lab
www.sciencedirect.com/science/arti...
Check out our latest work, featuring beautiful, first-of-their-kind cryo-tomograms of crucial plant cell wall modifications, Casparian strips, suberin lamellae, lignified xylem walls and more! From our Electron Microscopy Facility led by @chgenoud.bsky.social
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Imaging of specialized plant cell walls by improved cryo-CLEM and cryo-electron tomography
Cryo-focused ion beam scanning electron microscopy (cryo-FIBSEM) has become essential for preparing electron-transparent lamellae from cryo-plunged and high-pressure frozen specimens. However, targeti...
www.biorxiv.org
June 5, 2025 at 12:00 AM
Check out our latest work, featuring beautiful, first-of-their-kind cryo-tomograms of crucial plant cell wall modifications, Casparian strips, suberin lamellae, lignified xylem walls and more! From our Electron Microscopy Facility led by @chgenoud.bsky.social
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Dr. Eva Nogales is a structural biologist whose pioneering work in cryo-electron microscopy has revealed the intricate architecture of cellular machinery. @nationalacademies.org
More: explorers.com/eva-nogales/
More: explorers.com/eva-nogales/
December 27, 2025 at 1:53 PM
Dr. Eva Nogales is a structural biologist whose pioneering work in cryo-electron microscopy has revealed the intricate architecture of cellular machinery. @nationalacademies.org
More: explorers.com/eva-nogales/
More: explorers.com/eva-nogales/
... check out the latest pre-print by @claudioalfieri.bsky.social & his team!
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
Cryo-tides: synthetic peptides for cryo-EM grid preparation of multi-subunit protein complexes sensitive to the air-water interface
Cryogenic electron microscopy (cryo-EM) has enabled rapid progress in structural biology throughout the last decade. However, cryo-EM sample preparation still represents a major bottleneck in high-res...
www.biorxiv.org
October 1, 2026 at 11:57 AM
... check out the latest pre-print by @claudioalfieri.bsky.social & his team!
www.biorxiv.org/content/10.6...
www.biorxiv.org/content/10.6...
In a new Science study, cryo–electron tomography captures the in-cell architecture of the mitochondrial respiratory chain, illuminating how the coordinated action of molecular machines drives life’s fundamental energy conversion.
Learn more in this week's issue: scim.ag/3FA3Ygq
Learn more in this week's issue: scim.ag/3FA3Ygq
March 20, 2025 at 6:05 PM
In a new Science study, cryo–electron tomography captures the in-cell architecture of the mitochondrial respiratory chain, illuminating how the coordinated action of molecular machines drives life’s fundamental energy conversion.
Learn more in this week's issue: scim.ag/3FA3Ygq
Learn more in this week's issue: scim.ag/3FA3Ygq
It's out! 🥳
In situ architecture of #plasmodesmata - plant cytoplasmic bridges - by #cryoET.
📝 Paper: www.nature.com/articles/s41...
🧵 Thread: bsky.app/profile/madi...
#teamtomo #proteomics #AlphaFold
In situ architecture of #plasmodesmata - plant cytoplasmic bridges - by #cryoET.
📝 Paper: www.nature.com/articles/s41...
🧵 Thread: bsky.app/profile/madi...
#teamtomo #proteomics #AlphaFold
May 14, 2026 at 11:07 AM
It's out! 🥳
In situ architecture of #plasmodesmata - plant cytoplasmic bridges - by #cryoET.
📝 Paper: www.nature.com/articles/s41...
🧵 Thread: bsky.app/profile/madi...
#teamtomo #proteomics #AlphaFold
In situ architecture of #plasmodesmata - plant cytoplasmic bridges - by #cryoET.
📝 Paper: www.nature.com/articles/s41...
🧵 Thread: bsky.app/profile/madi...
#teamtomo #proteomics #AlphaFold
...a lot of people have asked me about our cryo-tides and how they help with preserving particles during cryo-EM grid preparation...and here we go #cryoem: www.biorxiv.org/content/10.6...
Cryo-tides: synthetic peptides for cryo-EM grid preparation of multi-subunit protein complexes sensitive to the air-water interface
Cryogenic electron microscopy (cryo-EM) has enabled rapid progress in structural biology throughout the last decade. However, cryo-EM sample preparation still represents a major bottleneck in high-res...
www.biorxiv.org
October 1, 2026 at 11:39 AM
...a lot of people have asked me about our cryo-tides and how they help with preserving particles during cryo-EM grid preparation...and here we go #cryoem: www.biorxiv.org/content/10.6...
Happy to say that my Current Opinion in Structural Biology article came out today. I hope it helps to guide to you in using cryo-ET to make new biological discoveries!
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
A practical look at cryo-electron tomography image processing: Key considerations for new biological discoveries
Cryo-electron tomography (cryo-ET) enables 3D visualization of complex biological environments without the need for purification, thereby preserving t…
www.sciencedirect.com
July 8, 2025 at 9:44 PM
Happy to say that my Current Opinion in Structural Biology article came out today. I hope it helps to guide to you in using cryo-ET to make new biological discoveries!
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Cryo-ET is often framed as a tool for in situ protein structure. But what if the real revolution is contextualization?
I explore how #teamtomo is redefining what "local" means in organelle biology, revealing membrane states rather than just protein structures.
tinyurl.com/localmembrane
I explore how #teamtomo is redefining what "local" means in organelle biology, revealing membrane states rather than just protein structures.
tinyurl.com/localmembrane
Think globally, act locally: Redefining organellar membrane environments through cryo-electron tomography
Early enthusiasm for the “cellular revolution” in cryo-electron tomography (cryo-ET) was largely driven by the promise of resolving protein structures…
www.sciencedirect.com
June 16, 2026 at 6:08 PM
Cryo-ET is often framed as a tool for in situ protein structure. But what if the real revolution is contextualization?
I explore how #teamtomo is redefining what "local" means in organelle biology, revealing membrane states rather than just protein structures.
tinyurl.com/localmembrane
I explore how #teamtomo is redefining what "local" means in organelle biology, revealing membrane states rather than just protein structures.
tinyurl.com/localmembrane
In print today @science.org Laser phase plate improves structure determination of small proteins by cryo-EM | Science www.science.org/doi/10.1126/...
Laser phase plate improves structure determination of small proteins by cryo-EM
Phase plates can, in principle, overcome the poor image contrast in cryo–electron microscopy (cryo-EM) and the resulting limits on the structural reconstruction of small proteins. However, previous de...
www.science.org
July 9, 2026 at 6:07 PM
In print today @science.org Laser phase plate improves structure determination of small proteins by cryo-EM | Science www.science.org/doi/10.1126/...
Me at the cryo-electron microscope:
"Ah yes, the big coffee maker."
"Ah yes, the big coffee maker."
July 8, 2026 at 3:58 AM
Me at the cryo-electron microscope:
"Ah yes, the big coffee maker."
"Ah yes, the big coffee maker."
I am super happy to share this paper in its final form. We used FIB milling to "dig in to" cell lines and mouse brains infected with tick-borne flaviviruses, followed by cryo-ET to study the virus replication. It's open access, so have a look!
#virology #teamtomo
www.nature.com/articles/s41...
#virology #teamtomo
www.nature.com/articles/s41...
Cryo-electron tomography reveals coupled flavivirus replication, budding and maturation - Nature Communications
In this study, Dahmane et al use a method called cryo-electron tomography to uncover new details of how tick-borne flaviviruses transform cells into virus factories.
www.nature.com
January 23, 2026 at 4:47 PM
I am super happy to share this paper in its final form. We used FIB milling to "dig in to" cell lines and mouse brains infected with tick-borne flaviviruses, followed by cryo-ET to study the virus replication. It's open access, so have a look!
#virology #teamtomo
www.nature.com/articles/s41...
#virology #teamtomo
www.nature.com/articles/s41...
I have lots of opinions—but for your sake, I’ve limited them to segmentation in cryo-ET for this article 😉
www.sciencedirect.com/science/arti...
#teamtomo
www.sciencedirect.com/science/arti...
#teamtomo
Segmenting cryo-electron tomography data: Extracting models from cellular landscapes
Cryo-electron tomography provides an unprecedented view of cellular architecture, yet extracting meaningful biological insights remains challenging. S…
www.sciencedirect.com
July 11, 2025 at 12:46 AM
I have lots of opinions—but for your sake, I’ve limited them to segmentation in cryo-ET for this article 😉
www.sciencedirect.com/science/arti...
#teamtomo
www.sciencedirect.com/science/arti...
#teamtomo
Exciting times for our lab- recent developments in cryoET tools and workflows now allow us apply these to study microbes in the host context. #teamtomo
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Beyond resolution: Cryo-electron tomography of microbial nanomachines in native host context
Cryo-electron tomography (cryo-ET) has revolutionized visualization of bacterial nanomachines by revealing molecular structures in situ under near-nat…
www.sciencedirect.com
May 8, 2026 at 10:12 AM
Exciting times for our lab- recent developments in cryoET tools and workflows now allow us apply these to study microbes in the host context. #teamtomo
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Extremely excited for the second version of this workshop at the MPI of Biophysics l, Frankfurt, that will happen 12-20. April 2026. Absolutely amazing lineup of speakers, I am very much looking forward to learning from everyone! Apply here: meetings.embo.org/event/26-in-...
August 15, 2025 at 4:09 PM
Extremely excited for the second version of this workshop at the MPI of Biophysics l, Frankfurt, that will happen 12-20. April 2026. Absolutely amazing lineup of speakers, I am very much looking forward to learning from everyone! Apply here: meetings.embo.org/event/26-in-...
Our colleagues Vinith Kishore and Valentin Debarnot from the @ivandokmanic.bsky.social lab have come up with an amazing deep learning tool for denoising and filling the missing wedge in #cryoET data. I'm pleased to introduce Icecream🍧
Icecream: High-Fidelity Equivariant Cryo-Electron Tomography
Cryo-electron tomography (cryo-ET) visualizes 3D cellular architecture in near-native states. Recent deep-learning methods (CryoCARE, IsoNet, DeepDeWedge, CryoLithe) improve denoising and artifact cor...
www.biorxiv.org
October 23, 2025 at 11:13 AM
Our colleagues Vinith Kishore and Valentin Debarnot from the @ivandokmanic.bsky.social lab have come up with an amazing deep learning tool for denoising and filling the missing wedge in #cryoET data. I'm pleased to introduce Icecream🍧
Visualizing influenza A virus assembly by in situ cryo-electron tomography
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Visualizing influenza A virus assembly by in situ cryo-electron tomography - Nature Communications
Influenza A virus must package eight separate genomic segments, called viral ribonucleoproteins (vRNPs). Using in situ cryo-electron tomography, the authors visualize how vRNPs are clustered on cell m...
www.nature.com
October 23, 2025 at 2:01 PM
Visualizing influenza A virus assembly by in situ cryo-electron tomography
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Cryo-electron tomography of appressoria of Magnaporthe introduced by @delaconcepcionjc.bsky.social #Fungal26
March 21, 2026 at 11:16 PM
Cryo-electron tomography of appressoria of Magnaporthe introduced by @delaconcepcionjc.bsky.social #Fungal26
AI for Cryo-ET 🌟 Our new paper presents a deep-learning framework that cleans up faulty tilt series in a more automated, precise and powerful way! 💪 Read more here: www.sciencedirect.com/science/arti...
Automated removal of corrupted tilts in cryo-electron tomography
Cryo-electron tomography (cryo-ET) enables the visualization of macromolecular structures in their near-native cellular environment. However, acquired…
www.sciencedirect.com
July 25, 2025 at 10:14 AM
AI for Cryo-ET 🌟 Our new paper presents a deep-learning framework that cleans up faulty tilt series in a more automated, precise and powerful way! 💪 Read more here: www.sciencedirect.com/science/arti...
Hey #TeamTomo,
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!
tomoguide.github.io
Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!
tomoguide.github.io
Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Welcome to TomoGuide
A step-by-step Cryo-ET guide
tomoguide.github.io
May 6, 2025 at 4:27 PM
Hey #TeamTomo,
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!
tomoguide.github.io
Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!
tomoguide.github.io
Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Paper alert🚨
Label your protein of interest and localize it in cryoET 👁️❄️
Genetically encoded FerriTag as a specific label for cryo-electron tomography: Structure www.cell.com/structure/fu...
Label your protein of interest and localize it in cryoET 👁️❄️
Genetically encoded FerriTag as a specific label for cryo-electron tomography: Structure www.cell.com/structure/fu...
Genetically encoded FerriTag as a specific label for cryo-electron tomography
Wang et al. present an optimized labeling strategy for cryo-electron tomography based
on genetically encoded FerriTag. This system uses rapamycin to recruit iron-loaded
ferritin to target proteins, en...
www.cell.com
September 11, 2025 at 9:36 AM
Paper alert🚨
Label your protein of interest and localize it in cryoET 👁️❄️
Genetically encoded FerriTag as a specific label for cryo-electron tomography: Structure www.cell.com/structure/fu...
Label your protein of interest and localize it in cryoET 👁️❄️
Genetically encoded FerriTag as a specific label for cryo-electron tomography: Structure www.cell.com/structure/fu...
More than 1'800 tomograms available for the community. Towards visual proteomics using our favorite organism Chlamy!
Check out the preprint: www.biorxiv.org/content/10.1...
Find it on EMPIAR (11830) and fully annotated on the CZI data portal: cryoetdataportal.czscience.com
#TeamTomo
Check out the preprint: www.biorxiv.org/content/10.1...
Find it on EMPIAR (11830) and fully annotated on the CZI data portal: cryoetdataportal.czscience.com
#TeamTomo
Towards community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii
In situ cryo-electron tomography (cryo-ET) has emerged as the method of choice to investigate structures of biomolecules in their native context. However, challenges remain in the efficient production...
www.biorxiv.org
December 29, 2024 at 11:47 AM
More than 1'800 tomograms available for the community. Towards visual proteomics using our favorite organism Chlamy!
Check out the preprint: www.biorxiv.org/content/10.1...
Find it on EMPIAR (11830) and fully annotated on the CZI data portal: cryoetdataportal.czscience.com
#TeamTomo
Check out the preprint: www.biorxiv.org/content/10.1...
Find it on EMPIAR (11830) and fully annotated on the CZI data portal: cryoetdataportal.czscience.com
#TeamTomo
Cryo-EM: the revolution continues doi.org/10.1107/S205...
Cryo-EM: the revolution continues
The increasing democratization and implementation of electron cryo-microscopy appears poised to drive a new revolution in digital structural biology.
doi.org
June 9, 2026 at 6:02 PM
Cryo-EM: the revolution continues doi.org/10.1107/S205...