#embryoid
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sdomcke.bsky.social
Expanding Perturb-seq-like screens to multicellular systems also increases the complexity of confounding effects. We encountered this when we perturbed all TFs in mosaic embryoid bodies (EBs) and develop a scalable solution by barcoding monoclonal individuals. 🧵 www.biorxiv.org/content/10.1...
Barcoded monoclonal embryoids are a potential solution to confounding bottlenecks in mosaic organoid screens
Genetic screens in organoids hold tremendous promise for accelerating discoveries at the intersection of genomics and developmental biology. Embryoid bodies (EBs) are self-organizing multicellular str...
www.biorxiv.org
May 25, 2025 at 11:00 AM
isdb.bsky.social
A whole embryoid is wild. Check out this review by Makoto Asashima et al.
cellsdev.bsky.social Cells & Development @cellsdev.bsky.social · Jan 31
A fascinating review on the role of Activin in organ induction. Isn't it wild that in Xenopus embryos, a piece of the animal cap can be induced with Activin at different concentrations and buffers to form the ❤️, kidney, the pancreas, head, tail, and even a whole embryoid 🤯:
doi.org/10.1016/j.cd...
Fig. 1. Animal cap assay and sandwich method as in vitro induction systems. In amphibians, a blastocoel cavity clearly forms inside the animal hemisphere during the blastula and early gastrula stages. The cap-like portion lining the roof of the blastocoel cavity is the animal cap. This region consists of a sheet of pluripotent cells, organized into one or several layers. In the animal cap assay, the animal cap was treated with a physiological saline solution containing inducing factors and then cultured. Depending on the type, concentration, and duration of exposure to the inducing factors, animal caps can differentiate into various cell types. In contrast, the sandwich method, involves culturing the inducer source in between two animal caps. In this technique, the sources of induction can include the dorsal lip of the blastopore (organizer), adult tissues, pelletized soluble factors, or animal caps pretreated with soluble factors. In this figure, activin is used as an example of an inducing factor. Fig. 12. Summary of the in vitro induction system using activin as an inducing factor. This in vitro induction system utilizes activin and retinoic acid as inducing factors to treat animal caps, employing techniques such as animal cap assay, dissociation/reaggregation protocol, and the sandwich method. By applying these methods, various levels of self-organization can be replicated and controlled in vitro, ranging from lower-order cell differentiation to higher-order tissue differentiation, organogenesis, and even the formation of fundamental body plans. Abbreviations: Dorsal [D], ventral [V], and retinoic acid [RA]. Fig. 11. Formation of embryoids by artificial activin concentration gradients. To create embryoids, animal caps were prepared through treatment with low (0.5–1 ng/ml), intermediate (5–10 ng/ml), or high (50–100 ng/ml) concentrations of activin. These three types of activin-treated animal caps were then sequentially arranged and cultured with untreated animal caps. After 3 days of culture, embryoids with distinct head and trunk-tail structures were formed (A). Histological sections revealed differentiation into head tissues, such as the cement gland [cg] and eyes, and trunk-tail tissues including the ear vesicle [ev], brain [br], notochord [not], muscle [mus], and gut (B). When newt embryos are used in similar combination cultures, neural plate structures forming the brain [white arrow] and axial structures forming the trunk-tail regions [black arrow] are sometimes observed (C). Fig. 7. In vitro heart formation and in vivo transplantation experiment. When treated with a high concentration of activin, the animal caps of Xenopus embryos did not differentiate into heart tissue. However, if the animal cap dissociates into individual cells before activin treatment and then reaggregates, it forms a beating heart [arrow] with 100 % efficiency (A). This heart expresses differentiation marker genes, such as Nkx2.5, GATA-4, Tbx5, MHCα, TnIc (cardiac troponin I), and ANF, none of which are expressed in an animal cap treated with activin alone, without dissociation/reaggregation (B). Electron microscopy reveals the presence of intercalated discs [id] specific to the cardiac muscle, along with visible mitochondria [m] and Z-bands [z] (C). When the reaggregated heart tissue is orthotopically transplanted into the cardiac primordium of a neurula-stage embryo, it integrates without rejection and continues to beat (D), although it does not persist through host metamorphosis. In contrast, when the reaggregated tissue is ectopically transplanted into the ventral region of the neurula, it begins to beat synchronously with the host heart and gradually reddens as it initiates blood circulation (E).
January 31, 2026 at 6:40 PM
dannimklee.bsky.social
New Preprint alert!🚨
Super excited to share my postdoc work now on BioRxiv.
How does PRC2 loss lead to developmental failures?
We combined rapid protein degradation with TLS embryoid models to uncover temporal and lineage-specific dependencies 🧬.
Preprint: doi.org/10.64898/2026.04.18.719361
April 24, 2026 at 8:58 AM
natbiotech.nature.com
The August issue is live www.nature.com/nbt/volumes/...

The cover shows human pluripotent stem cell–derived embryoid bodies differentiating in vitro into hematopoietic stem cells and, after transplantation in mice, into blood cell lineages go.nature.com/3XeDg1Q
August 12, 2025 at 4:28 PM
pilarcacheiro.bsky.social
S Domcke. Barcoded monoclonal embryoid bodies allow quantification of the consequences of TF perturbations and inter-individual heterogeneity.

#eshg2026
June 14, 2026 at 12:32 PM
carmeloferrai.bsky.social
Fantastic opportunity!!! Apply and embrace the opportunity to have so much fun answering a bunch of exciting biological questions using embryoid models.
nmoris.bsky.social Naomi Moris @nmoris.bsky.social · Feb 20
🧫Looking for a PhD to start Autumn 2025? We're recruiting! 🧪

The project will focus on using #embryomodels to explore #development & could cover a range of specific questions. Please RT! 🙏

Apply via the portal, and get more details here: www.crick.ac.uk/careers-stud...
February 20, 2025 at 3:13 PM
bacell3d.bsky.social
4 days left until the registration deadline for #BaCell3D 2025!

Click here to join us at the forefront of #organoids research:
conferences.unibas.ch/frontend/ind...

#embryoid #assembloid #tumoroid #NAMs #gastruloid #stemcells #tissueengineering #reduce #replace #refine #3Rs #3Dmodels #organonchip
April 24, 2025 at 5:07 PM
jeroenpasterkamp.bsky.social
Check out this paper by Werner Dykstra in which we study the effects of GFAP mutations on brain organoid development. doi.org/10.1002/glia...
Mutations in GFAP Alter Early Lineage Commitment of Organoids
Mutations in GFAP alter lineage commitment of organoids. The severity of this commitment defect is dependent on the method of embryoid body formation. GFAP is expressed in iPSCs and during early st...
doi.org
July 30, 2025 at 2:00 PM
syncelljoshua.bsky.social
#HiSciSky
It's great to be on! My name is Josh Hislop, and I'm a graduate student at the University of Pittsburgh doing work on developmental biology, and specifically stem cell based embryo models. I really enjoy investigating the fundamental stages of blood development and organ patterning!
An immunofluorescence image of the heX-Embryoid stem cell based embryo model, showing the development of blood progenitor-like cells surrounded by a yolk sac-like tissue.
November 25, 2023 at 11:18 PM
pasquelab.bsky.social
Continuous modeling of primate embryogenesis from totipotency to early organogenesis. Cell. www.cell.com/cell/abstrac...
Continuous modeling of primate embryogenesis from totipotency to early organogenesis
A primate embryoid system is established from cynomolgus totipotent blastomere-like cells. This fertilization-independent system recapitulates development from a totipotent-like state through an inter...
www.cell.com
June 11, 2026 at 7:35 PM
biorxiv-neursci.bsky.social
3D organoids containing endothelial and neural cells generation by serial inductions of differentiation on human iPSC-derived embryoid bodies https://www.biorxiv.org/content/10.1101/2025.05.20.653559v1
May 21, 2025 at 9:23 PM
aurifexsds.bsky.social
Skoyz = Nicolas Ussel, Stéphane Ayas (also of Embryoid, Liyr), Stéphane Buffavand
"Liquid Mutation" - 1996

French electro-industrial project active through 2010

#EBMBits
youtu.be/DFJfu1zSYGs
Sofistik 8 - Liquid Mutation (1996)
YouTube video by mysteriuminiquitatis
youtu.be
August 1, 2026 at 9:04 PM
aineurolab.bsky.social
* the burrata looks a bit like an organoid. Or as we used to call them, embryoid bodies.
December 24, 2024 at 5:21 PM
pierreosteil.bsky.social
New type of oganoid: "Emojioid". #FluorescentTuesday
🧪🔬👨‍🔬
An embryoid body made of embryonic stem cells displaying a smiling face!
December 10, 2024 at 1:06 PM
kprummel.bsky.social
Looking for an ultra-high-throughput single-cell RNA+ATAC method? 🎆

Check out our SUM-seq method, now officially out! 🧬

Showcased on macrophages, T cells, and iPSC-derived embryoid bodies; more cell types and tissues to come! 🧫

Reach out for the detailed protocol! 📝 ✨
May 26, 2025 at 10:58 AM
enardhellmannwg.bsky.social
New paper from the group!
📃 doi.org/10.7554/eLif...
We investigated orthologous cell types in primate EBs and found that marker genes don't transfer well across species.
Check it out for insights into cross-species scRNA-seq and explore the data in our shiny app:
🔎 shiny.bio.lmu.de/Cross_Specie...
Identification and comparison of orthologous cell types from primate embryoid bodies shows limits of marker gene transferability
doi.org
April 7, 2025 at 12:58 PM
elife.bsky.social
Identification and comparison of orthologous cell types from primate embryoid bodies shows limits of marker gene transferability
Identification and comparison of orthologous cell types from primate embryoid bodies shows limits of marker gene transferability
Analysis of scRNA-seq data from primate embryoid bodies highlights the value of cross-species comparisons for identifying and characterizing cell types, as marker genes can evolve fast.
buff.ly
April 21, 2026 at 8:02 PM
cp-cell.bsky.social
Now online! Continuous modeling of primate embryogenesis from totipotency to early organogenesis
Continuous modeling of primate embryogenesis from totipotency to early organogenesis
A primate embryoid system is established from cynomolgus totipotent blastomere-like cells. This fertilization-independent system recapitulates development from a totipotent-like state through an intermediate 8CMLC stage to early organogenesis with neural-tube-like structures and cardiac-like beating activity.
dlvr.it
June 10, 2026 at 10:38 PM
amartinezarias.bsky.social
Most important, a need for rigor in the outcome of the culture. There's no point in saying an “embryo cultured to 15, 16, 20 days” if what one has is a mass of cells, resembling an embryoid body rather than a structured embryo.
July 6, 2025 at 8:08 AM
gokberkalagoz.bsky.social
In @eLife.bsky.social: Identification and comparison of orthologous cell types from primate embryoid bodies shows limits of marker gene transferability doi.org/10.7554/eLif...
Identification and comparison of orthologous cell types from primate embryoid bodies shows limits of marker gene transferability
doi.org
March 10, 2026 at 4:51 PM
geneticsmike7.bsky.social
13/ Here's the commonly used H1 ESC background, differentiated into microglia with an embryoid body protocol (we used EB and monolayer)
November 2, 2025 at 2:29 PM
yyingchenyy.bsky.social
New type of self-assembloid? Once again, amazed at how rapidly the field of stem-cell-based embryoid models is developing.
nobuhamazaki.bsky.social Nobu Hamazaki @nobuhamazaki.bsky.social · Sep 26
New paper from my lab and @jshendure.bsky.social lab! Led by the brilliant @zukailiu.bsky.social and @cxqiu.bsky.social. We tackled how anterior and posterior progenitor cells cooperate to self-organize into an embryonic structure (termed AP-gastruloid). (1/n) www.biorxiv.org/content/10.1...
September 27, 2025 at 9:49 AM
poslfit.bsky.social
NASPA Word of the Day: RIBOZYME n a molecule of RNA that functions as an enzyme. Hook:ribozymeS. Blanagrams:biomeTry bromizeD bromizeS embryoiD. https://scrabbleplayers.org/cgi-bin/wotd.pl
March 22, 2025 at 12:55 PM
ertrunnell.bsky.social
By @ellyhol.bsky.social
@jeroenpasterkamp.bsky.social
@lukasvalihrach.bsky.social
and colleagues

Also from:
@czechacademy.bsky.social
@unc-cbp.bsky.social
@cib-csic.bsky.social
@lundstem.bsky.social
@cityofhope.bsky.social

Read more:
onlinelibrary.wiley.com/doi/10.1002/...
Mutations in GFAP Alter Early Lineage Commitment of Organoids
Mutations in GFAP alter lineage commitment of organoids. The severity of this commitment defect is dependent on the method of embryoid body formation. GFAP is expressed in iPSCs and during early st...
onlinelibrary.wiley.com
September 11, 2025 at 12:39 PM
choijunhong.bsky.social
(Side note: Sam also used this approach to generate monoclonal embryoid bodies for perturbation screening with @sdomcke.bsky.social and @cxqiu.bsky.social.) bsky.app/profile/sdom... (6/13)
sdomcke.bsky.social Silvia Domcke @sdomcke.bsky.social · May 25
Expanding Perturb-seq-like screens to multicellular systems also increases the complexity of confounding effects. We encountered this when we perturbed all TFs in mosaic embryoid bodies (EBs) and develop a scalable solution by barcoding monoclonal individuals. 🧵 www.biorxiv.org/content/10.1...
Barcoded monoclonal embryoids are a potential solution to confounding bottlenecks in mosaic organoid screens
Genetic screens in organoids hold tremendous promise for accelerating discoveries at the intersection of genomics and developmental biology. Embryoid bodies (EBs) are self-organizing multicellular str...
www.biorxiv.org
May 27, 2025 at 2:57 PM