#hiPSCs
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willowcoyote.bsky.social
Extraordinary work by Jess Mella. In a technical tour de force she got over nearly 20k variants into hipscs to study the effects in an important nuclear envelope protein LMNA. She used it to dive into cell, structure, and human genetics biology. Keep an eye on her and her future is bright!
September 22, 2026 at 3:00 PM
ablanchasensio.bsky.social
With Christmas just around the corner, it’s time for the #FluorescenceFriday post🎄!

This week, we’re featuring a hiPSC reporter line engineered to express 2 fluorescent proteins—located in the nuclei and actin filaments of #hiPSCs, generated using STRAIGHT-IN Dual🧪🔬

@benjaminbjohnson.bsky.social?
December 13, 2024 at 2:19 PM
the-node.bsky.social
Featured image with Özge Özgüç: the Node–FocalPlane image competition

Find out more about @oozguc.bsky.social and the story behind "Cell-estial bloom", which won the image competition we organised with @focalplane.bsky.social:
thenode.biologists.com/featured-ima...
A ‘Cell-estial Bloom’ of human induced pluripotent stem cells (hiPSCs) flourishes on a micropatterned island. This image presents a colony of live hiPSCs, with fluorescently labeled Lamin B delineating the nuclear lamina within each cell. Acquired with a Zeiss LSM 880 Airyscan microscope, this maximum intensity projection is enhanced with depth-coded coloring to reveal the captivating three-dimensional landscape.
May 7, 2025 at 8:22 AM
martinowk.bsky.social
With expansion of several stem cell based initiatives @lieberinstitute.bsky.social - we are hiring! We are looking for a Staff Scientist with expertise in hiPSCs and iPSC-derived neuronal models to join our Stem Cell Core, directed by @tsawada1.bsky.social

recruiting.paylocity.com/recruiting/j...
August 12, 2026 at 5:26 PM
oozguc.bsky.social
🎄 𝗜𝘁’𝘀 𝗕𝗲𝗴𝗶𝗻𝗻𝗶𝗻𝗴 𝘁𝗼 𝗟𝗼𝗼𝗸 𝗮 𝗟𝗼𝘁 𝗟𝗶𝗸𝗲 𝗖𝗵𝗿𝗶𝘀𝘁𝗺𝗮𝘀... 𝗨𝗻𝗱𝗲𝗿 𝘁𝗵𝗲 𝗠𝗶𝗰𝗿𝗼𝘀𝗰𝗼𝗽𝗲! 🔬

Why buy Christmas trees when you can "grow" one using micropatterning and @alleninstitute.bsky.social cells that come with their own LaminB1 "ornaments"?

Happy #FluorescenceFriday! ✨❄️
A colony of human induced pluripotent stem cells (hiPSCs) in a christmas tree-like shape. The cells' nuclear envelope appears in magenta in the image forming the ornaments. In green, we see the actin structures within the cells, giving the colony a tree like texture.
December 13, 2024 at 9:37 AM
tajbakhshlab.bsky.social
Our Stem Cell Course #ASCB is back! After 2nd day, @institutpasteur.bsky.social @tajbakhshlab.bsky.social, @zenlabpasteur.bsky.social, Barbara Gayraud-Morel, and great interactive students. Laurence Daheron, Frank Yates, Michel Cohen-Tannoudji covered pluripotency and generating hiPSCs. Great team!
June 24, 2026 at 5:24 AM
trayon.bsky.social
🔥💣 rewirement of hiPSCs towards “anterior epiblast” restores differentiation capacity 🔥💣

Congrats to both teams!!!
August 7, 2026 at 5:03 PM
ablanchasensio.bsky.social
Here is my 2nd #FluorescenceFriday post! 🧪 #hiPSCs expressing jRCaMP1b, a genetically-encoded Ca2+ indicator 👻. This reporter allows us to record intracellular Ca2+ in hiPSC-derived #cardiomyocytes and cardioids (videos in the comments) #microscopy 🔬 #Science #Biology @benjaminbjohnson.bsky.social
Genetically-modified hiPSCs expressing the genetically-encoded calcium indicator jRCaMP1b with a nuclear export signal (NES). This reporter is used to track calcium dynamics in live cells. It combines a red fluorescent protein with calmodulin and a calcium-binding domain, changing fluorescence intensity upon calcium binding. Its red-shifted emission is ideal for imaging in complex tissues or dual-color experiments, making it valuable for studying calcium-mediated processes. A single copy of this reporter was integrated using the STRAIGHT-IN platform at the AAVS1 locus. See below! https://doi.org/10.1016/j.crmeth.2022.100300 https://doi.org/10.1101/2024.10.17.616637
December 6, 2024 at 2:59 PM
the-node.bsky.social
🎉Congratulations to the Top 3 images from the Node–FocalPlane image competition!

1️⃣"Cell-estial bloom" by @oozguc.bsky.social
2️⃣"Dancing actinotroch" by @allancarbal.bsky.social
3️⃣"Who’s active?" by @jupeloggia.bsky.social

thenode.biologists.com/results-from...

@focalplane.bsky.social #biologists100
"Cell-estial bloom" Image from Özge Özgüç: A ‘Cell-estial bloom’ of human induced pluripotent stem cells (hiPSCs) flourishes on a micropatterned island. This image presents a colony of live hiPSCs, with fluorescently labelled Lamin B delineating the nuclear lamina within each cell. Acquired with a Zeiss LSM 880 Airyscan microscope, this maximum intensity projection is enhanced with depth-coded coloring to reveal the captivating three-dimensional landscape. "Dancing actinotroch" by Allan Carrillo-Baltodano: Actinotroch larva of a phoronid worm with phalloidin shown in yellow and acetylated tubulin in magenta. Imaged with a Zeiss LSM 800 at 10 x magnification. "Who’s active?" by Julia Peloggia de Castro: The image depicts a zebrafish embryo at 9 hours post-fertilisation on a lateral view. Cells are stained with MitoTracker, which labels active mitochondria, and cell membranes are labelled in cyan with a EGFP transgenic membrane tag. Image was taken using a 20x objective on a spinning disk confocal microscope.
March 27, 2025 at 10:01 AM
lab-turner.bsky.social
We are excited to share our latest preprint led by Ruta Meleckyte and Waz Varsally! www.biorxiv.org/content/10.1... We generated a set of human isogenic induced pluripotent stem cells (hiPSCs) that are autosomally identical but differ in sex chromosome composition: XX, XY or monosomic X
A human induced pluripotent stem cell toolbox for studying sex chromosome effects
Sex chromosomes shape male (XY) - female (XX) differences in development and disease. These differences can be modelled in vitro by comparing XY and XX human induced pluripotent stem cells (hiPSCs). H...
www.biorxiv.org
March 7, 2025 at 10:36 AM
oozguc.bsky.social
My image is shortlisted and will be displayed at #100Biologists! 🎉

This “Cell-estial Bloom” features LaminB-labeled cells from @alleninstitute.bsky.social glowing in all their fluorescent glory! ✨

All very incredible images—check them out and vote for your favorite (Hope it’s mine! 🤞)!
a colony of live hiPSCs, with fluorescently labeled Lamin B delineating the nuclear lamina within each cell. this maximum intensity projection is enhanced with depth-coded coloring to reveal the captivating three-dimensional landscape.
March 12, 2025 at 2:21 PM
akispapantonis.bsky.social
Our preprint on how variant U1 snRNAs control transcriptional and splicing homeostasis of hiPSCs is now officially out in @natcomms.nature.com and accessible via this link:
www.nature.com/articles/s41...
This has been the culmination of our work as part of the SPP1935 program funded by @dfg.de

1/n
May 13, 2026 at 8:50 AM
meleckmas.bsky.social
Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation: Stem Cell Reports www.cell.com/stem-cell-re...

Sharing on behalf of my coauthors, delighted to have played a small role in this study
Gene reactivation upon erosion of X chromosome inactivation in female hiPSCs is predictable yet variable and persists through differentiation
In this article, da Rocha and colleagues show that XCI erosion is frequent yet variable in female human iPSCs, leading to reactivation of some X-linked genes, including escapees. This reactivation is ...
www.cell.com
April 6, 2025 at 10:46 PM
stemcellreports.bsky.social
New research shows X-chromosome inactivation (XCI) erosion is frequent and variable in female hiPSCs, leading to reactivation of several X-linked genes, like escape genes.

@isscr.org @cellpress.bsky.social @gairdnerawards.bsky.social @sickkidsvs.bsky.social

ow.ly/H4kC50Vuf12
April 7, 2025 at 2:05 PM
faulknerlab.bsky.social
Read this preprint today from @jakobssonlab.bsky.social lab that profiles and CRISPRi perturbs L1 transcription in hiPSCs and cerebral organoids. It is extremely well done. The L1 CRISPRi phenotype in the organoids is subtle and variable but interesting. 1/n

www.biorxiv.org/content/10.1...

#TEsky
LINE-1 retrotransposons regulate the exit of human pluripotency and early brain development
Long interspersed nuclear element 1 (L1) retrotransposons represent a vast source of divergent genetic information. However, mechanistic analysis of whether and how L1s contribute to human development...
www.biorxiv.org
February 13, 2025 at 2:18 AM
vallierlab.bsky.social
The latest publication from @vallierlab.bsky.social is now online. This tour de force from the amazing Laetitia Pinte (supervised by @martavilagonz.bsky.social) uncover the function of EHF in the lung (not a liver story!) epithelium using hiPSCs, genome editing, and lung organoids
July 24, 2025 at 9:07 AM
jmella.bsky.social
3/ We built a landing pad platform to introduce ~18,000 cDNA variants into a safe harbor locus of hiPSCs, then differentiated them into cardiomyocytes and epicardial progenitor cells to assay how cardiac cells handle mutated lamin proteins.
September 21, 2026 at 9:33 PM
craigagoodman1.bsky.social
Tissue-engineered neuromuscular organoids
www.nature.com/articles/s42...
Tissue-engineered neuromuscular organoids - Communications Biology
We developed tissue-engineered neuromuscular organoids by combining decellularized skeletal muscle and hiPSCs. The provided ECM scaffolds improved cell maturation, enabling the study of (dys)functiona...
www.nature.com
July 20, 2025 at 5:44 PM
carmeloferrai.bsky.social
Integrin β1 activity controls colony morphology during human pluripotent stem cell state transitions

www.cell.com/stem-cell-re...
Integrin β1 activity controls colony morphology during human pluripotent stem cell state transitions
Taskinen et al. describe unprecedented integrin-dependent regulation of PSC states. They report that integrin β1 inhibition induces a rounded naive-like colony morphology in hiPSCs and attenuates colo...
www.cell.com
July 28, 2025 at 4:45 AM
labwaggoner.bsky.social
@biorxivpreprint.bsky.social Efficient Generation of Functional TCRαβ+ Cytotoxic T Cells from hiPSCs via Small-Molecule Modulation
www.biorxiv.org/content/10.6... @bostonchildrens.bsky.social
April 5, 2026 at 11:09 PM
molinalab.bsky.social
And finally! We produced a scRNA-seq time course of hiPSCs differentiating into definitive endoderm, and we found that lineage commitment remodels the cell cycle in a coordinated way.
September 24, 2026 at 8:25 AM
ablanchasensio.bsky.social
Calcium signals in beating hiPSCs-derived cardiomyocytes.
A single copy of this reporter was integrated using the STRAIGHT-IN platform at the AAVS1 locus.
doi.org/10.1016/j.cr...
doi.org/10.1101/2024...
December 6, 2024 at 3:01 PM
akispapantonis.bsky.social
…so Kostas generated vU1-specific KOs using CRISPR-Cas9 in hiPSCs of the two most expressed variants, and Yajie analyzed their effects via total RNA-seq data, while @carmeloferrai.bsky.social provided undirected differentiation tests.

3/n
September 16, 2025 at 8:37 AM
cp-cellrepmethods.bsky.social
Self-amplifying RNA enables rapid, durable, integration-free programming of hiPSCs
Self-amplifying RNA enables rapid, durable, integration-free programming of hiPSCs
Self-amplifying RNAs (saRNAs) have the potential for rapid, durable, non-integrating delivery of transgenes in human induced pluripotent stem cells. Della Santina et al. show that transcription factors and reporters expressed from saRNA are present for multiple weeks, supporting forward programming to neurons and tracking electrophysiological properties in 3D cardiac spheroids.
dlvr.it
September 16, 2026 at 6:49 PM
jcb.org
🔬 From our May issue: Immunofluorescence of human induced pluripotent #StemCells (hiPSCs)-derived cardiac myocytes acquired with a confocal microscope (Leica SP8). Cyan, lamin A/C; gray, DAPI (DNA); FIRE, troponin.
From Kaitlyn M. Shen et al. @pennepigenetics.bsky.social: doi.org/10.1083/jcb....
May 18, 2026 at 8:31 PM