Obvs. someone is preparing the neuron-specific isoform of ole yeller enzyme.
September 28, 2026 at 6:10 PM
Obvs. someone is preparing the neuron-specific isoform of ole yeller enzyme.
😮
Whole- #transcriptome -scale isoform-resolved spatial imaging of single #cells in tissues: Cell www.cell.com/cell/fulltex...
Whole- #transcriptome -scale isoform-resolved spatial imaging of single #cells in tissues: Cell www.cell.com/cell/fulltex...
Whole-transcriptome-scale isoform-resolved spatial imaging of single cells in tissues
RT&T-AMP-MERFISH enables whole-transcriptome-scale, isoform-resolved spatial imaging
of single cells in intact tissues and reveals how gene programs and transcript isoform
usage vary across cell types...
www.cell.com
September 28, 2026 at 9:41 PM
😮
Whole- #transcriptome -scale isoform-resolved spatial imaging of single #cells in tissues: Cell www.cell.com/cell/fulltex...
Whole- #transcriptome -scale isoform-resolved spatial imaging of single #cells in tissues: Cell www.cell.com/cell/fulltex...
A multi-regional human brain atlas of chromatin accessibility and gene expression facilitates promoter-isoform resolution genetic fine-mapping
www.nature.com/articles/s41...
www.nature.com/articles/s41...
A multi-regional human brain atlas of chromatin accessibility and gene expression facilitates promoter-isoform resolution genetic fine-mapping - Nature Communications
Brain gene regulation is key for neuropsychiatric disorders. Here, the authors show that profiling gene expression and chromatin states in 25 brain regions enables enhancer-promoter mapping at isoform...
www.nature.com
November 23, 2024 at 12:09 PM
A multi-regional human brain atlas of chromatin accessibility and gene expression facilitates promoter-isoform resolution genetic fine-mapping
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Our paper using Oxford #Nanopore direct RNA sequencing to identify m6A modifications on RNA isoforms in human brain is now out in Science Advances. 🧪
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Isoform-level profiling of m6A epitranscriptomic signatures in human brain
Direct RNA-seq in brain reveals RNA isoform and region-specific m6A modifications, highlighting their role in gene regulation.
www.science.org
August 12, 2025 at 2:30 AM
Our paper using Oxford #Nanopore direct RNA sequencing to identify m6A modifications on RNA isoforms in human brain is now out in Science Advances. 🧪
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Nature research paper: Single-cell and isoform-specific translational profiling of the mouse brain
go.nature.com/4aYPQu7
go.nature.com/4aYPQu7
Single-cell and isoform-specific translational profiling of the mouse brain - Nature
Post-transcriptional regulation of mRNA translation was explored using Ribo-STAMP and single-cell RNA sequencing to reveal cell-type-specific and isoform-specific translation patterns across hippocampal neuronal and non-neuronal cell types, highlighting functional differences between CA1 and CA3.
go.nature.com
February 21, 2026 at 11:34 AM
Nature research paper: Single-cell and isoform-specific translational profiling of the mouse brain
go.nature.com/4aYPQu7
go.nature.com/4aYPQu7
BOOM! Great stuff on the truncated TrkB isoform in nociception and nociceptors from @shivanibr.bsky.social's lab #painresearch www.biorxiv.org/content/10.1...
Truncated TrkB: The predominant TrkB Isoform in Nociceptors
Truncated form of the tropomyosin receptor kinase B isoform (TrkBT1), traditionally considered a dominant-negative regulator of full-length TrkB (TrkBTK+), remains poorly understood in peripheral sens...
www.biorxiv.org
December 27, 2024 at 11:54 PM
BOOM! Great stuff on the truncated TrkB isoform in nociception and nociceptors from @shivanibr.bsky.social's lab #painresearch www.biorxiv.org/content/10.1...
RNA splicing generates a functionally specialized Rep protein isoform in geminiviruses — enabling timely control of the viral cycle. Strikingly, similar strategies might have evolved in DNA viruses infecting different kingdoms: www.biorxiv.org/content/10.6... Spearheaded by @delphinem-p.bsky.social!
February 20, 2026 at 9:31 AM
RNA splicing generates a functionally specialized Rep protein isoform in geminiviruses — enabling timely control of the viral cycle. Strikingly, similar strategies might have evolved in DNA viruses infecting different kingdoms: www.biorxiv.org/content/10.6... Spearheaded by @delphinem-p.bsky.social!
Our new paper in @plosbiology.org introduces IntAct-U-ExM enabling isoform-specific imaging of actin at nanoscale across yeast, mammalian cells & primary neurons. Fabulous work spearheaded by
@anubhavdhar.bsky.social along with an amazing team! #actin #U-ExM journals.plos.org/plosbiology/...
@anubhavdhar.bsky.social along with an amazing team! #actin #U-ExM journals.plos.org/plosbiology/...
June 12, 2026 at 7:34 PM
Our new paper in @plosbiology.org introduces IntAct-U-ExM enabling isoform-specific imaging of actin at nanoscale across yeast, mammalian cells & primary neurons. Fabulous work spearheaded by
@anubhavdhar.bsky.social along with an amazing team! #actin #U-ExM journals.plos.org/plosbiology/...
@anubhavdhar.bsky.social along with an amazing team! #actin #U-ExM journals.plos.org/plosbiology/...
Imaging-based spatial isoform transcriptomics resolves transcript diversity at cellular and subcellular resolution https://www.biorxiv.org/content/10.64898/2026.09.22.753429v1
September 28, 2026 at 5:15 PM
Imaging-based spatial isoform transcriptomics resolves transcript diversity at cellular and subcellular resolution https://www.biorxiv.org/content/10.64898/2026.09.22.753429v1
Preprint: IntAct-U-ExM enables robust, isoform-specific expansion microscopy of actin networks in yeast and mammalian cells. A cool study led by @anubhavdhar.bsky.social in collaboration with Sudarshan and Deepak Nair. www.biorxiv.org/content/10.1...
May 16, 2025 at 8:00 AM
Preprint: IntAct-U-ExM enables robust, isoform-specific expansion microscopy of actin networks in yeast and mammalian cells. A cool study led by @anubhavdhar.bsky.social in collaboration with Sudarshan and Deepak Nair. www.biorxiv.org/content/10.1...
Excited for this to be out officially! It was a great team effort and has a lot of useful tidbits for studying isoform function. www.nature.com/articles/s41...
Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolbox - Nature Communications
The majority of human genes can produce multiple isoforms, but studying their functional relevance requires tools to target specific isoforms. Here, the authors develop a CRISPR-based exon-exon juncti...
www.nature.com
July 29, 2025 at 4:35 PM
Excited for this to be out officially! It was a great team effort and has a lot of useful tidbits for studying isoform function. www.nature.com/articles/s41...
Cross-tissue isoform switches by human transcription factors suggest a widespread regulatory mechanism https://www.biorxiv.org/content/10.1101/2024.11.28.625692v1
Cross-tissue isoform switches by human transcription factors suggest a widespread regulatory mechanism https://www.biorxiv.org/content/10.1101/2024.11.28.625692v1
Many human genes, including transcription factor (TF) genes, produce alternative coding isoforms. Al
www.biorxiv.org
November 29, 2024 at 7:34 PM
Cross-tissue isoform switches by human transcription factors suggest a widespread regulatory mechanism https://www.biorxiv.org/content/10.1101/2024.11.28.625692v1
"Here, we identify an alternative splicing isoform of human PD-1, which carries a 28-base pairs extension retained from 5′ region of intron 2 (PD-1^28), is expressed in peripheral T cells and tumor infiltrating lymphocytes."
#Immunology #Immunotherapy
www.nature.com/articles/s41...
#Immunology #Immunotherapy
www.nature.com/articles/s41...
A splicing isoform of PD-1 promotes tumor progression as a potential immune checkpoint - Nature Communications
Whether PD-1 splicing isoforms impact T cell anti-tumor capacity has not been fully illustrated. Here the authors identify a human PD-1 isoform, PD-1^28, which functions to suppress anti-cancer immuni...
www.nature.com
November 29, 2024 at 9:32 PM
"Here, we identify an alternative splicing isoform of human PD-1, which carries a 28-base pairs extension retained from 5′ region of intron 2 (PD-1^28), is expressed in peripheral T cells and tumor infiltrating lymphocytes."
#Immunology #Immunotherapy
www.nature.com/articles/s41...
#Immunology #Immunotherapy
www.nature.com/articles/s41...
Very happy to share our pre-print co-led with Nathan Andrews where we reveal splicing and isoform changes in a single-cell CRISPRi screen. For example, GEMIN5 knockdown produced minimal gene expression changes yet extensively reshaped the isoform landscape.
With my postdocs Nathan Andrews and @josiegleeson.bsky.social , we demonstrate the importance and analyze methods for isoform-level resolution in single cell-CRISPR perturbations. TL;DR: Long reads are the way. 3/3 www.biorxiv.org/content/10.6...
Isoform-level resolution in single-cell CRISPR screens reveals hidden functional consequences of gene perturbation
Single-cell CRISPR screens have enabled systematic investigation of gene function, but studies have largely focused on gene-level effects, overlooking transcriptional complexity and isoform usage. Met...
www.biorxiv.org
July 12, 2026 at 3:18 PM
Very happy to share our pre-print co-led with Nathan Andrews where we reveal splicing and isoform changes in a single-cell CRISPRi screen. For example, GEMIN5 knockdown produced minimal gene expression changes yet extensively reshaped the isoform landscape.
Chuffed to see our paper with @geneyeo.bsky.social finally published in @nature.com.
www.nature.com/articles/s41...
Very grateful to Fede, Susie, and Dave from my lab, and Sammi, Eric, and Pratibha from Gene's lab. What did we find? ⬇️ ⬇️ ⬇️
www.nature.com/articles/s41...
Very grateful to Fede, Susie, and Dave from my lab, and Sammi, Eric, and Pratibha from Gene's lab. What did we find? ⬇️ ⬇️ ⬇️
Single-cell and isoform-specific translational profiling of the mouse brain - Nature
Post-transcriptional regulation of mRNA translation was explored using Ribo-STAMP and single-cell RNA sequencing to reveal cell-type-specific and isoform-specific translation patterns across hipp...
www.nature.com
February 24, 2026 at 6:03 PM
Chuffed to see our paper with @geneyeo.bsky.social finally published in @nature.com.
www.nature.com/articles/s41...
Very grateful to Fede, Susie, and Dave from my lab, and Sammi, Eric, and Pratibha from Gene's lab. What did we find? ⬇️ ⬇️ ⬇️
www.nature.com/articles/s41...
Very grateful to Fede, Susie, and Dave from my lab, and Sammi, Eric, and Pratibha from Gene's lab. What did we find? ⬇️ ⬇️ ⬇️
Splicing impairment reprograms the transcription start site of STING, switching its expression from an antiviral isoform to a nonantiviral variant that partially suppresses STING activity @pnas.org
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
September 27, 2026 at 6:49 PM
Splicing impairment reprograms the transcription start site of STING, switching its expression from an antiviral isoform to a nonantiviral variant that partially suppresses STING activity @pnas.org
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
1/ 🚀 AEBP2 isn’t what we thought.
You were told that AEBP2 promotes PRC2 activity on chromatin.
We found the opposite: the most prevalent AEBP2 isoform inhibits PRC2 activity.
👉 surl.li/cgwqcq
A thread 🧵
You were told that AEBP2 promotes PRC2 activity on chromatin.
We found the opposite: the most prevalent AEBP2 isoform inhibits PRC2 activity.
👉 surl.li/cgwqcq
A thread 🧵
October 31, 2025 at 10:53 AM
1/ 🚀 AEBP2 isn’t what we thought.
You were told that AEBP2 promotes PRC2 activity on chromatin.
We found the opposite: the most prevalent AEBP2 isoform inhibits PRC2 activity.
👉 surl.li/cgwqcq
A thread 🧵
You were told that AEBP2 promotes PRC2 activity on chromatin.
We found the opposite: the most prevalent AEBP2 isoform inhibits PRC2 activity.
👉 surl.li/cgwqcq
A thread 🧵
RNA isoform-resolved multiplexed sequencing with bioorthogonal barcoding https://www.biorxiv.org/content/10.64898/2026.09.22.753383v1
September 28, 2026 at 3:33 PM
RNA isoform-resolved multiplexed sequencing with bioorthogonal barcoding https://www.biorxiv.org/content/10.64898/2026.09.22.753383v1
Thrilled to share our work on transcription initiation and termination being spatially coordinated out today in Science! www.science.org/doi/10.1126/...
mRNA initiation and termination are spatially coordinated
Transcriptional initiation and termination decisions drive messenger RNA (mRNA) isoform diversity but the relationship between them remains poorly understood. By systematically profiling joint usage o...
www.science.org
October 10, 2025 at 6:14 PM
Thrilled to share our work on transcription initiation and termination being spatially coordinated out today in Science! www.science.org/doi/10.1126/...
The "kitchen sink" of omics to solve the basis for an undiagnosed disease: long read genome , transcriptome, methytome, epigenome, all synchronized (a first)
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Synchronized long-read genome, methylome, epigenome and transcriptome profiling resolve a Mendelian condition - Nature Genetics
Simultaneous profiling of the genome, methylome, epigenome and transcriptome using single-molecule chromatin fiber sequencing and multiplexed arrays isoform sequencing identifies the genetic and molec...
www.nature.com
January 29, 2025 at 2:45 PM
The "kitchen sink" of omics to solve the basis for an undiagnosed disease: long read genome , transcriptome, methytome, epigenome, all synchronized (a first)
www.nature.com/articles/s41...
www.nature.com/articles/s41...
RNAkinet identifies nascent RNA by predicting 5EU metabolically labeled #RNA from raw #nanopore electrical signal. Should allow association of RNA metabolism dynamics with isoform usage, poly(A) tails and native RNA mods. academic.oup.com/nargab/artic...
November 24, 2024 at 2:01 AM
RNAkinet identifies nascent RNA by predicting 5EU metabolically labeled #RNA from raw #nanopore electrical signal. Should allow association of RNA metabolism dynamics with isoform usage, poly(A) tails and native RNA mods. academic.oup.com/nargab/artic...
"Beyond Gene Expression: Single-Cell Transcriptomics at Isoform Resolution"
by Lan Lin & colleagues
authors.elsevier.com/sd/article/S...
by Lan Lin & colleagues
authors.elsevier.com/sd/article/S...
July 9, 2026 at 1:30 PM
"Beyond Gene Expression: Single-Cell Transcriptomics at Isoform Resolution"
by Lan Lin & colleagues
authors.elsevier.com/sd/article/S...
by Lan Lin & colleagues
authors.elsevier.com/sd/article/S...
Our paper in Journal of Pain challenges old labels: TrkB isnt just an A-LTMR marker. Its truncated isoform is expressed and functional in trigeminal nociceptors, opening new avenues for studying the receptor in pathological pain #painresearch www.sciencedirect.com/science/arti...
Truncated TrkB: The predominant TrkB isoform in nociceptors
Truncated TrkB (TrkBT1), traditionally considered a dominant-negative regulator of full-length TrkB (TrkBTK+), remains poorly understood in peripheral…
www.sciencedirect.com
May 17, 2025 at 10:49 PM
Our paper in Journal of Pain challenges old labels: TrkB isnt just an A-LTMR marker. Its truncated isoform is expressed and functional in trigeminal nociceptors, opening new avenues for studying the receptor in pathological pain #painresearch www.sciencedirect.com/science/arti...
The first publication while at UCLA! We developed and utilized generative AI protein design methods for targeting the disordered and highly charged C-terminus of RAS to generate RAS isoform specific binders/inhibitors: www.sciencedirect.com/science/arti...
De novo design of Ras isoform selective binders
The four major isoforms encoded by RAS proto-oncogenes are differentially associated with cancer, but there are few isoform-specific binding reagents …
www.sciencedirect.com
March 11, 2026 at 8:20 PM
The first publication while at UCLA! We developed and utilized generative AI protein design methods for targeting the disordered and highly charged C-terminus of RAS to generate RAS isoform specific binders/inhibitors: www.sciencedirect.com/science/arti...