We’re excited to announce the opening of the Geneva Expansion Microscopy Facility (GenExM)!
GenExM is a full-service U-ExM platform (U-ExM, Cryo-ExM, iU-ExM), delivering nanoscale imaging.
Led by Dr. Olivier Mercey, GenExM welcomes academic & industrial collaborations.
👉 www.unige.ch/genexm/
GenExM is a full-service U-ExM platform (U-ExM, Cryo-ExM, iU-ExM), delivering nanoscale imaging.
Led by Dr. Olivier Mercey, GenExM welcomes academic & industrial collaborations.
👉 www.unige.ch/genexm/
December 10, 2025 at 8:43 PM
We’re excited to announce the opening of the Geneva Expansion Microscopy Facility (GenExM)!
GenExM is a full-service U-ExM platform (U-ExM, Cryo-ExM, iU-ExM), delivering nanoscale imaging.
Led by Dr. Olivier Mercey, GenExM welcomes academic & industrial collaborations.
👉 www.unige.ch/genexm/
GenExM is a full-service U-ExM platform (U-ExM, Cryo-ExM, iU-ExM), delivering nanoscale imaging.
Led by Dr. Olivier Mercey, GenExM welcomes academic & industrial collaborations.
👉 www.unige.ch/genexm/
Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
August 28, 2025 at 7:03 AM
Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social
Our new paper in @plosbiology.org introduces IntAct-U-ExM enabling isoform-specific imaging of actin at nanoscale across yeast, mammalian cells & primary neurons. Fabulous work spearheaded by
@anubhavdhar.bsky.social along with an amazing team! #actin #U-ExM journals.plos.org/plosbiology/...
@anubhavdhar.bsky.social along with an amazing team! #actin #U-ExM journals.plos.org/plosbiology/...
June 12, 2026 at 7:34 PM
Our new paper in @plosbiology.org introduces IntAct-U-ExM enabling isoform-specific imaging of actin at nanoscale across yeast, mammalian cells & primary neurons. Fabulous work spearheaded by
@anubhavdhar.bsky.social along with an amazing team! #actin #U-ExM journals.plos.org/plosbiology/...
@anubhavdhar.bsky.social along with an amazing team! #actin #U-ExM journals.plos.org/plosbiology/...
Happy #FluorescenceFriday all!
Checkout these beautiful basal bodies in airway #multiciliated cells expanded via U-ExM 😍
Images captured by the super talented @maneesha07.bsky.social on a #Nikon NSPARC 🔬.
Checkout these beautiful basal bodies in airway #multiciliated cells expanded via U-ExM 😍
Images captured by the super talented @maneesha07.bsky.social on a #Nikon NSPARC 🔬.
March 13, 2026 at 4:41 PM
Happy #FluorescenceFriday all!
Checkout these beautiful basal bodies in airway #multiciliated cells expanded via U-ExM 😍
Images captured by the super talented @maneesha07.bsky.social on a #Nikon NSPARC 🔬.
Checkout these beautiful basal bodies in airway #multiciliated cells expanded via U-ExM 😍
Images captured by the super talented @maneesha07.bsky.social on a #Nikon NSPARC 🔬.
I am excited to share our most recent work collaborating with @centriolelab.bsky.social and @stearnslab.bsky.social to look at the ciliary base of mammalian multiciliated cells w/ cryo-ET, XL/MS, and U-ExM www.biorxiv.org/content/10.6...
April 8, 2026 at 6:25 AM
I am excited to share our most recent work collaborating with @centriolelab.bsky.social and @stearnslab.bsky.social to look at the ciliary base of mammalian multiciliated cells w/ cryo-ET, XL/MS, and U-ExM www.biorxiv.org/content/10.6...
Happy #FluorescenceFriday all! 🔬
First Chlamydomonas experiment/U-ExM image from my lab. Haven't worked on these beautiful little cells since grad school 😍 #memories #cilia
First Chlamydomonas experiment/U-ExM image from my lab. Haven't worked on these beautiful little cells since grad school 😍 #memories #cilia
May 1, 2026 at 2:31 PM
Happy #FluorescenceFriday all! 🔬
First Chlamydomonas experiment/U-ExM image from my lab. Haven't worked on these beautiful little cells since grad school 😍 #memories #cilia
First Chlamydomonas experiment/U-ExM image from my lab. Haven't worked on these beautiful little cells since grad school 😍 #memories #cilia
Our collaboration with @mbonhivers.bsky.social is now out in @plosbiology.org! 🎉Using U-ExM, we mapped the formation of the flagellar pocket collar in T. brucei. Huge thanks to everyone involved — one step closer to #PhDone!
#ExM #oneringtorulethemall #Bilbo1 #trypanosoma #protistsonsky
#ExM #oneringtorulethemall #Bilbo1 #trypanosoma #protistsonsky
The flagellar pocket collar (FPC) is a cytoskeletal structure essential for nutrient uptake & immune evasion in #Trypanosome. @mbonhivers.bsky.social &co use U-ExM to provide novel insights into FPC biogenesis, and reveal 2 unknown cytoskeletal structures @plosbiology.org 🧪 plos.io/4n3bWi6
October 10, 2025 at 6:47 PM
Our collaboration with @mbonhivers.bsky.social is now out in @plosbiology.org! 🎉Using U-ExM, we mapped the formation of the flagellar pocket collar in T. brucei. Huge thanks to everyone involved — one step closer to #PhDone!
#ExM #oneringtorulethemall #Bilbo1 #trypanosoma #protistsonsky
#ExM #oneringtorulethemall #Bilbo1 #trypanosoma #protistsonsky
🔬🔦Our Imaging spotlight from @hiralshah.bsky.social & team highlights their U-ExM atlas of planktonic eukaryotes, including details on how you can explore this wonderful resource. Plus, check out Hiral’s top tips on getting started with expansion microscopy.
focalplane.biologists.com/2026/01/20/i...
focalplane.biologists.com/2026/01/20/i...
Imaging spotlight: U-ExM atlas of microbial eukaryotes - FocalPlane
Imaging spotlight: U-ExM atlas of microbial eukaryotes - News
focalplane.biologists.com
January 20, 2026 at 1:26 PM
🔬🔦Our Imaging spotlight from @hiralshah.bsky.social & team highlights their U-ExM atlas of planktonic eukaryotes, including details on how you can explore this wonderful resource. Plus, check out Hiral’s top tips on getting started with expansion microscopy.
focalplane.biologists.com/2026/01/20/i...
focalplane.biologists.com/2026/01/20/i...
🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
June 19, 2025 at 8:58 AM
🚨 New preprint!
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
Using U-ExM + in situ cryo-ET, we show how C2CD3 builds an in-to-out radial architecture connecting the distal centriole lumen to its appendages. Great collab with @cellarchlab.com @chgenoud.bsky.social @stearnslab.bsky.social 🙌. #TeamTomo #UExM
www.biorxiv.org/content/10.1...
This is a glimpse of the cytoskeletal structures we found doing U-ExM in more than 200 species, now in Cell🤩: shorturl.at/6oHvi
Amazing collaboration between @centriolelab.bsky.social , @dudinlab.bsky.social and @gautamdey.bsky.social labs.
I believe last one is a 🕷️
#FluorescenceFriday
#Microscopy
Amazing collaboration between @centriolelab.bsky.social , @dudinlab.bsky.social and @gautamdey.bsky.social labs.
I believe last one is a 🕷️
#FluorescenceFriday
#Microscopy
November 7, 2025 at 1:35 PM
This is a glimpse of the cytoskeletal structures we found doing U-ExM in more than 200 species, now in Cell🤩: shorturl.at/6oHvi
Amazing collaboration between @centriolelab.bsky.social , @dudinlab.bsky.social and @gautamdey.bsky.social labs.
I believe last one is a 🕷️
#FluorescenceFriday
#Microscopy
Amazing collaboration between @centriolelab.bsky.social , @dudinlab.bsky.social and @gautamdey.bsky.social labs.
I believe last one is a 🕷️
#FluorescenceFriday
#Microscopy
Preprint: IntAct-U-ExM enables robust, isoform-specific expansion microscopy of actin networks in yeast and mammalian cells. A cool study led by @anubhavdhar.bsky.social in collaboration with Sudarshan and Deepak Nair. www.biorxiv.org/content/10.1...
May 16, 2025 at 8:00 AM
Preprint: IntAct-U-ExM enables robust, isoform-specific expansion microscopy of actin networks in yeast and mammalian cells. A cool study led by @anubhavdhar.bsky.social in collaboration with Sudarshan and Deepak Nair. www.biorxiv.org/content/10.1...
🤩 Another amazing example of U-ExM actin staining with HAK-actin by @dudinlab.bsky.social et. al.
At this high resolution, we also caught something new: Using the new #HAK-Actin #UExM probe, we saw that
#Actin accumulates at the furrow base before compartments fully seal ➡️ they're still open at the bottom.
➡️ The #multicellular epithelium-like stage is shorter than we previously proposed.
#Actin accumulates at the furrow base before compartments fully seal ➡️ they're still open at the bottom.
➡️ The #multicellular epithelium-like stage is shorter than we previously proposed.
March 18, 2026 at 12:28 PM
🤩 Another amazing example of U-ExM actin staining with HAK-actin by @dudinlab.bsky.social et. al.
Interested in applying U-ExM to cells, tissues, organoids, or cryo-sections? Our EMBO workshop is open for applications!
🧫🔬 Deadline: 12 Jan 2026 — don’t miss it!
🧫🔬 Deadline: 12 Jan 2026 — don’t miss it!
Have you applied for 'Ultrastructure expansion microscopy: from cells to tissue' yet? #EMBOExM
🔬 Advance your microscopy skills by joining us to explore U-ExM techniques and gain hands-on expertise in applying this powerful approach to mammalian cells.
Apply now: s.embl.org/uex26-01-bl
🔬 Advance your microscopy skills by joining us to explore U-ExM techniques and gain hands-on expertise in applying this powerful approach to mammalian cells.
Apply now: s.embl.org/uex26-01-bl
December 2, 2025 at 3:13 PM
Interested in applying U-ExM to cells, tissues, organoids, or cryo-sections? Our EMBO workshop is open for applications!
🧫🔬 Deadline: 12 Jan 2026 — don’t miss it!
🧫🔬 Deadline: 12 Jan 2026 — don’t miss it!
🧬 New tool for U-ExM!
HAK-Actin is now available from Spirochrome → a reliable probe to label actin filaments in expansion microscopy.
Check it out at Spirochrome!
#UExM #Actin #Cytoskeleton #SuperResolution
HAK-Actin is now available from Spirochrome → a reliable probe to label actin filaments in expansion microscopy.
Check it out at Spirochrome!
#UExM #Actin #Cytoskeleton #SuperResolution
Discover HAK-actin™: A New Tool To Visualize The Actin Cytoskeleton by Expansion Microscopy
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
• Stronger signal via post-expansion labeling
• Broad cell lines & species compatibility
• Endogenous actin labeling
• Can be multiplexed with other targets
spirochrome.com/product/hak-...
September 10, 2025 at 9:59 AM
🧬 New tool for U-ExM!
HAK-Actin is now available from Spirochrome → a reliable probe to label actin filaments in expansion microscopy.
Check it out at Spirochrome!
#UExM #Actin #Cytoskeleton #SuperResolution
HAK-Actin is now available from Spirochrome → a reliable probe to label actin filaments in expansion microscopy.
Check it out at Spirochrome!
#UExM #Actin #Cytoskeleton #SuperResolution
Are you interested in the nuclear envelope and are still lacking a dye for it? Try the lectin concanavalin A in #U-ExM! Together with @baukjeje.bsky.social, @gautamdey.bsky.social and @dudinlab.bsky.social and many collaborators sharing species with us we explored the versatility of this dye
We wanted to share this asap for everyone to use ! This will remain a #preprint for the time being. We are happy to recieve feedback, hear whether or not ot works in your hands and continue to apply it across #eukaryotes as part of #ExÂME
You want to know more :
www.dudinlab.com/exame
You want to know more :
www.dudinlab.com/exame
Research
ExÂME is the Expansion Microscopy Atlas of Microbial Eukaryotes, a community-driven project uncovering the hidden cellular architectures of protists and microbial eukaryotes. Supported by the Moore Fo...
www.dudinlab.com
September 11, 2026 at 8:00 AM
Are you interested in the nuclear envelope and are still lacking a dye for it? Try the lectin concanavalin A in #U-ExM! Together with @baukjeje.bsky.social, @gautamdey.bsky.social and @dudinlab.bsky.social and many collaborators sharing species with us we explored the versatility of this dye
Expansion microscopy is revolutionary but application to actin cytoskeleton has been challenging. Not anymore....we combine IntAct + U-ExM to get an unprecedented 3D nanoscale view of actin isoforms in expanded cells. Read the full revised paper here: plos.io/4vansgr
June 15, 2026 at 10:23 PM
Expansion microscopy is revolutionary but application to actin cytoskeleton has been challenging. Not anymore....we combine IntAct + U-ExM to get an unprecedented 3D nanoscale view of actin isoforms in expanded cells. Read the full revised paper here: plos.io/4vansgr
Day 2 of the EMBO U-ExM Workshop at EMBL Heidelberg #EMBOExM. Gels are ready and expanding nicely! We also had a lively poster session and inspiring talks from @sabsalon.bsky.social and @gautamdey.bsky.social. A very engaged group, great to see such thoughtful discussions around expansion microscopy
April 22, 2026 at 8:57 AM
Day 2 of the EMBO U-ExM Workshop at EMBL Heidelberg #EMBOExM. Gels are ready and expanding nicely! We also had a lively poster session and inspiring talks from @sabsalon.bsky.social and @gautamdey.bsky.social. A very engaged group, great to see such thoughtful discussions around expansion microscopy
Nice work "IntAct-U-ExM enables super-resolution imaging of isoform-specific actin networks across species": dx.plos.org/10.1371/jour...
👉endogenously tagged beta and gamma actin inside the nuclear compartment- how cool is that?!
👉endogenously tagged beta and gamma actin inside the nuclear compartment- how cool is that?!
June 14, 2026 at 4:07 PM
Nice work "IntAct-U-ExM enables super-resolution imaging of isoform-specific actin networks across species": dx.plos.org/10.1371/jour...
👉endogenously tagged beta and gamma actin inside the nuclear compartment- how cool is that?!
👉endogenously tagged beta and gamma actin inside the nuclear compartment- how cool is that?!
If you’re looking to stain DNA in ExM and in particular U-ExM, Spirochrome offers a broad range of dyes that work really well 👍
Are you staining DNA in your ExM samples?
We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.
You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.
spirochrome.com/products/?_c...
We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.
You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.
spirochrome.com/products/?_c...
January 8, 2026 at 5:38 PM
If you’re looking to stain DNA in ExM and in particular U-ExM, Spirochrome offers a broad range of dyes that work really well 👍
Key transcripts encoded centrin-like proteins, a frequent component of sensory/contractile structures in protists. We visualized tentacle ultrastructure by U-ExM with @dudinlab.bsky.social lab, discovering stunning tip and collar structures 🤯that add structural complexity to new tentacle formation.
November 18, 2025 at 4:15 PM
Key transcripts encoded centrin-like proteins, a frequent component of sensory/contractile structures in protists. We visualized tentacle ultrastructure by U-ExM with @dudinlab.bsky.social lab, discovering stunning tip and collar structures 🤯that add structural complexity to new tentacle formation.
Our paper on U-ExM for mosquito tissues (MoTissU-ExM) in BMC methods!
Great collaboration with @sabsalon.bsky.social and @benliffner.bsky.social
bmcmethods.biomedcentral.com/articles/10....
Great collaboration with @sabsalon.bsky.social and @benliffner.bsky.social
bmcmethods.biomedcentral.com/articles/10....
Mosquito Tissue Ultrastructure-Expansion Microscopy (MoTissU-ExM) enables ultrastructural and anatomical analysis of malaria parasites and their mosquito - BMC Methods
Background Study of malaria parasite cell biology is challenged by their small size, which can make visualisation of individual organelles difficult or impossible using conventional light microscopy. ...
bmcmethods.biomedcentral.com
January 19, 2025 at 11:16 PM
Our paper on U-ExM for mosquito tissues (MoTissU-ExM) in BMC methods!
Great collaboration with @sabsalon.bsky.social and @benliffner.bsky.social
bmcmethods.biomedcentral.com/articles/10....
Great collaboration with @sabsalon.bsky.social and @benliffner.bsky.social
bmcmethods.biomedcentral.com/articles/10....
This is U-ExM (surface rendering)
September 26, 2025 at 4:15 PM
This is U-ExM (surface rendering)
An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™.
These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green).
Movie from preprint www.biorxiv.org/content/10.6...
🧪
These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green).
Movie from preprint www.biorxiv.org/content/10.6...
🧪
March 16, 2026 at 11:43 AM
An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™.
These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green).
Movie from preprint www.biorxiv.org/content/10.6...
🧪
These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green).
Movie from preprint www.biorxiv.org/content/10.6...
🧪
Our #JCSciliaSI is complete
Explore our ToC: journals.biologists.com/jcs/issue/13...
Our cover image shows tracheal epithelial cells from an air-liquid interface culture imaged using U-ExM from Oliver Mercey and Marine Brunet @centriolelab.bsky.social.
journals.biologists.com/jcs/article/...
Explore our ToC: journals.biologists.com/jcs/issue/13...
Our cover image shows tracheal epithelial cells from an air-liquid interface culture imaged using U-ExM from Oliver Mercey and Marine Brunet @centriolelab.bsky.social.
journals.biologists.com/jcs/article/...
November 4, 2025 at 1:36 PM
Our #JCSciliaSI is complete
Explore our ToC: journals.biologists.com/jcs/issue/13...
Our cover image shows tracheal epithelial cells from an air-liquid interface culture imaged using U-ExM from Oliver Mercey and Marine Brunet @centriolelab.bsky.social.
journals.biologists.com/jcs/article/...
Explore our ToC: journals.biologists.com/jcs/issue/13...
Our cover image shows tracheal epithelial cells from an air-liquid interface culture imaged using U-ExM from Oliver Mercey and Marine Brunet @centriolelab.bsky.social.
journals.biologists.com/jcs/article/...
HAK-actin™: a new probe to label #actin in your expansion microscopy samples with a robust yet simple protocol.
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE
September 19, 2025 at 9:45 AM
HAK-actin™: a new probe to label #actin in your expansion microscopy samples with a robust yet simple protocol.
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE
Find out how HAK-actin™ can help you achieve a bright actin staining in your ExM workflow: spirochrome.com/product/hak-...
Image credits: Olivier Mercey, UNIGE